• Journal of Life Science
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• v.22 no.8
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• pp.1046-1051
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• 2012

In order to investigate the changes in bioactive materials induced in goldfish brains by $^{18}F$ irradiation, the variations in the neurotransmitter levels in the whole brain were studied. The distance between the goldfish and 580 mCi of $^{18}F$ was about 4 cm, and the exposure lasted for 4 hrs. The absorption level calculated based on the distance, exposure time, and half-life of $^{18}F$ was approximately 2 Gy. After sacrifice by $^{18}F$ irradiation or untreated conditions, ten brains were dissected or immediately frozen, respectively. The tissues were extracted in acetic acid. After lyophilization, the samples were dissolved in distilled water and were further purified on a reverse-phase HPLC column. There were no differences in the intensities of the bioactive materials between $^{18}F$-exposed goldfish and control goldfish, while the only peak corresponded to 13 min, which indicated a significant increase in the irradiated brains. Our analysis has found that this compound is tryptophan. This result suggests that $^{18}F$ leads to changes in a classical neurotransmitter, tryptophan, in both the brains of control goldfish and goldfish contaminated by irradiation.

• Journal of Life Science
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• v.30 no.7
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• pp.592-597
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• 2020

Soybean [Glycine max (L.) Merr.] seeds contain an average of 40% protein on a dry weight basis, but they also contain antinutritional elements such as lectin, Kunitz trypsin inhibitor (KTI), and 7S α'- subunit protein. The objective of this research was to develop a new soybean genotype with triple recessive alleles for these elements. Three parents (Gaechuck#2, PI506876, and Le-16) were used to develop the genetic population, and the presence of lectin and KTI protein was detected using Western blot while 7S α' subunit protein was detected using SDS-PAGE. One F₃ plant strain with proper agronomical traits such as type, height, seed quality, and 100-seed weight was selected. The genotype of the developed strain is titilelecgy1cgy1, that is KTI, lectin, and 7S α' subunit protein free. The new strain has a purple flower, determinate growth habit, and light yellow pods at maturity. The seed has a buffer hilum and is yellow in color. The new strain's height was 58 cm compared to the Daewonkong cultivar at 46 cm, and its 100-seed weight was 27.1 g, smaller than the Daewonkong at 29.0 g. This is the first new soybean strain with the titilelecgy1cgy1 genotype, and it can be used to improve yellow soybean cultivars of high quality and function.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.9 no.4
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• pp.515-525
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• 1998

In this paper, for both transmission and reception with single antenna system of satellite communication, dual-frequency antennas which operate simultaneously at 12.5 GHz and at 14.25 GHz are designed, constructed and measured. Also by using dual feeding structure, the problems of single-fed dual-frequency antenna such as the separation of transmitting and receiving signals and single polarization, are solved. Microstrip patch as a radiation element of dual-fed dual-frequency antenna has width and length which are the resonance lengths of the corresponding frequencies for transmission and reception, respectively. The effects of the feed line on the other frequency feeding are minimized with the optimal matching scheme for the feed lines. For solving the space problems of dual-fed two-dimensional array antennas, microstrip-line and coaxial probe feedings are used for each frequency and a two-dimensional $2\times2$ array antenna was designed and measured their characteristics. The experimental results show that errors of resonance frequencies are less than 1.44%, the return losses are less than -21 dB and the isolations between two feeding ports are less than -21 dB. The characteristics of radiation patterns of dual frequency microstrip antenna are measured and evaluated. The directivities, sidelobe levels and cross polarizations are also measured and compared with the simulations. The results show some errors due to the misalignment of coaxial probe feeding.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.302-307
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• 2014

Many rhizobacteria can promote plant growth through various direct or indirect mechanisms, and their production of phytohormones such as indole-3-acetic acid (IAA) may have pronounced effects on growth and development of plants. Rhizobacterial strain isolated from rhizosphere of foxtail (Setaria viridis), Acinetobacter sp. SW5 produced 118.1 mg/L of IAA and 4.5 mg/L of gibberellin ($GA_3$) in brain heart broth medium at 2 and 1 day of incubation, respectively. In a pot test the lengths of stem and root and fresh weight of the germinated tomato seedlings treated with Acinetobacter sp. SW5 significantly increased by 26.3, 33.3, and 105.3%, respectively compared to those of the uninoculated control in 12 weeks of cultivation. When the root exudate secreted from tomato seedlings was analyzed by HPLC, 3.75 ng mg tomato $root^{-1}$ of tryptophan which is an IAA precursor was detected. Acinetobacter sp. SW5 could produce $4.06{\mu}M$ of IAA from root exudate from 8 tomato seedlings. Together with the capability of growth of Acinetobacter sp. SW5 in the tomato root exudates, this IAA secreted by bacteria might contribute to enhance the growth of tomato plants.

• Journal of Life Science
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• v.23 no.2
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• pp.182-189
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• 2013

Halophytes are unique land plants that are capable of thriving in a high-salt environment. They are attracting public attention due to their ability to synthesize bioactive substances such as UV protectants or antioxidizing agents. To achieve unaffected growth under high salinity, halophytes may take advantage of the activities of cell growth factors such as expansins. Expansins are well-known cell wall proteins that are responsible for cell enlargement. They loosen cell walls, thereby contributing to actual plant growth. This study aimed to identify positive roles of expansins in the growth of halophytes. Three expansin cDNA clones were isolated from seedlings of Suaeda japonica. Comparing the deduced amino acid sequences of the expansin genes of S. japonica with those of other plant species suggested that the cDNA clones isolated from S. japonica belong to the EXPA (${\alpha}$-expansin) gene family. A phylogenetic tree based on the deduced amino acid sequences revealed that the expansins of S. japonica share a close evolutionary relationship with those of strawberry (Fragaria ananassa) and jujube (Ziziphus jujuba), both of which are woody dicots. SjEXPAs did not show any remarkable change in the gene expression level in different NaCl concentrations, providing a clue to the unaffected seedling growth of S. japonica in a high-salt environment. In conclusion, the present study presents the first report of expansin genes from halophytes and suggests a putative role for these genes in plant growth under high salinity.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.29-33
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• 2010

Soybean (Glycine max (L.) Merr.) seed is the main source of protein and oil for human and animal. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are exist in the raw mature soybean. Lipoxygenase, Kunitz trypsin inhibitor (KTI) protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin are main antinutritional factors in soybean seed. Breeding of a new soybean strain with lacking these components is needed. The objective of this research was to select new soybean line with lipoxygenase-free, KTI-free, and ${\alpha}^{\prime}-subunit$ free (lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype). Total 434 $F_2$seeds were obtained from the cross of cultivar, "Gaechuck#2" and PI506876. Presence and absence of lipoxygenase, KTI protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin was tested by SDS electrophoresis using a partial seed of each $F_2$seed. Only one $F_2$seed with lacking these three components was selected and was planted to $F_2$plant. Absence of lipoxygenase, KTI, and ${\alpha}^{\prime}-subunit$ protein was confirmed on the $F_3$seeds harvested. Selected line with lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype might be useful for soybean breeding.

• Journal of the Korea Society of Computer and Information
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• v.18 no.11
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• pp.115-124
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• 2013

In this paper, we suggest a secure data dissemination by Lightweight Individual Encryption Multicast scheme over wireless sensor networks using the individual encryption method with Forward Error Correction instead of the group key encryption method. In wireless sensor networks, a sink node disseminates multicast data to the number of sensor nodes to update the up to date software such as network re-programming and here the group key encryption method is the general approach to provide a secure transmission. This group key encryption approach involves re-key management to provide a strong secure content distribution, however it is complicated to provide group key management services in wireless sensor networks due to limited resources of computing, storage, and communication. Although it is possible to control an individual node, the cost problem about individual encryption comes up and the individual encryption method is difficult to apply in multicast data transmission on wireless sensor networks. Therefore we only use 0.16% of individually encrypted packets to securely transmit data with the unicast to every node and the rest 99.84% non-encrypted encoded packets is transmitted with the multicast for network performance.

• Nuclear Engineering and Technology
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• v.18 no.2
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• pp.97-106
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• 1986

System thermal-hydraulic parameters and simulated, using the best-estimate system code(RELAPS/MODl/NSC), based upon the sequence of events for the KNU1 (Korea Nuclear Unit 1) loss of offsite power transient at 77.5% power which occurred on June 9,1981. The results are compared with the actual plant transient data and show good agreements. After the flow coastdown following the trips of both reactor coolant pumps, the establishment of natural circulation by the temperature difference between the hot and the cold legs is confirmed. The calculated reactor coolant flowrate closely approximates the plant data indicating the validity of relevant thermal-hydraulic models in the RELAP5/MOD1/NSC. Results also show that the sufficient heat removal capability is secured by the appropriate supply of the auxiliary feedwater without the operation of S/G PORVs. In addition, a scenario accident at full power, based upon the same sequence of events described above, is also analysed and the results confirmed that the safety of KNU1 is secured by the appropriate operation of the S/G PORVs coupled with the supply of auxiliary feedwater which ensures sufficient heat removal capability. The characteristics of the non-safety related components such as the turbine stop valve closing time, S/G PORV settings etc. are recognized to be important in the transient analyses on a bestestimate basis.

• Journal of Life Science
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• v.29 no.7
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• pp.774-778
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• 2019

Soybean [Glycine max (L.) Merr.] is one of the major food sources of protein, oil, carbohydrates, isoflavones, and other nutrients for both humans and animals. However, soybean seeds contain antinutritional factors, such as lectin protein, Kunitz Trypsin Inhibitor (KTI) protein, and stachyose. The objective of this research was to stack recessive alleles for development a triple recessive genotype, titilelers2rs2, with low KTI protein, lectin protein, and stachyose contents. Three parents (Gaechuck#2, PI200508, and 14G20) were used to develop the breeding population. The presence or absence of the lectin and KTI proteins was detected by western blotting. The stachyose content in mature seeds was determined by HPLC. Agronomic traits, such as plant type, plant height, maturity date, lodging, seed quality, and 100-seed weight, were evaluated for the four $F_3$ plant strains. One $F_4$ plant strain with the desired agronomical traits was selected. One new strain with the triple recessive titilelers2rs2 genotype was developed. The plant height of the new strain was 51 cm and the 100-seed weight was 31.0 g. The new strain had a yellow seed coat and yellow hilum. The stachyose content of the new strain was 3.8 g/kg. One strain developed in this research will be used to produce improved yellow soybean cultivars that are free of lectin and KTI proteins and low in stachyose content.

• Knowledge Management Research
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• v.24 no.1
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• pp.99-126
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• 2023

As cultural background contributes members of society to recognize and behave in a specific direction, customers with different cultural backgrounds show various reactions even when they are provided with the same service. Previous studies have used the Hofstede cultural dimension to understand how hotel visitors' satisfaction varies with the provided service as per their cultural background. However existing research only considered the cultural background of the guests, and there are not many studies focused on the types of travel. Therefore, in this study, the travel types of hotel visitors are classified into business travel visitors and leisure tourism visitors, and analyzed the effect of Hofstede's cultural dimension on hotel selection attributes according to the styles of travel. In this study, we collected information on six cultural dimensions of Hofstede, and from TripAdvisor, a representative tourism platform, 204,261 optional attribute ratings for hotels in New York to investigate the satisfaction of hotel selection attributes. In conclusion, it is expected that this study will be able to identify which service attributes the customers of various cultures who visit hotels put emphasis in advance, and therefore provide suitable service accordingly.