• Journal of Plant Biology
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• v.5 no.3
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• pp.5-10
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• 1962

Studies on the anatomical observation of Bamboos distributed in East-South Asia for systematic botany were scarecely reported except for the morphological studies on Bamboos, which was investigated by Dr. Hiroshi Usui, present author and others. Recently it has been certain that it could be hardly classify some species of bamboo family without this work. For this reason the author reported the anatomical studies on Korean bamboos in 1959, and furthermore the author intended to identify the two species of Pleioblastum Simoni Nakai in Korean bamboos by studying the characteristics of internal structure and qualitative assay of free amino acid containg in Pleioblastus Simoni Nakai(1), (2). In view of fact that there is some differences between two species, it is likely to be identifiable completely different species one another. Furthermore, a lot of characteristics surveyed by the author were as follow: It was reassumed that two species cultivated under same conditions for three years were different alternately in the species of Pleioblastus Simoni Nakai. At the same time, these studies could clarify some evolutional processes of bamboo family. On the other hand, it was found that two species had not only the obvious difference in aerenchyma, the size of thickwalled parenchyma and bulliform cell, but also the internode of Pleioblastus Simoni Nakai(1) is longer than the other, the speed of growing is more rapid, the leaf of former is narrow and that of latter is wide. The free amino acids containg in each species were found as 18 kinds, especially Pleioblastus Simoni Nakai (2) had not Histidine in spite of containg in Pleioblastus Simoni Nakia(1). From the characteristics and the experiments described above, it seemed to the author that Pleioblasus Simoni Nakai (1) which was growing at the region of Ulsan in Korea.

• Journal of Aquaculture
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• v.18 no.2
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• pp.115-121
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• 2005

This study was performed to investigate the formation and mass production of resting eggs by freshwater rotifer, Brachionus calyciflorus as influenced by different aeration supplements and exchange intervals of culture water in 15-L culture vessels and 500 L culture tanks. The maximum densities and mixis rates of the rotifers were not different between experimental group exposed to air or oxygen supplements. However, the fertilization rate and formation of resting eggs of the rotifers in the air-supplemented group were significantly higher than those in the oxygen supplemented group. In the experiment concerning exchange interval of culture water, the maximum density of the rotifers and formation of resting eggs in the batch culture were significantly higher than those in the semi-continuous culture with exchange of water every day. The formation of resting eggs per Chlorella dry weight was highest in the semi-continuous culture with exchange of water every day. The resting eggs of rotifers were produced at a density of $51.8{\sim}57.9{\times}10^6$ eggs in 500-L culture tanks. In this study, the batch culture with air is an effective method for mass production of resting eggs by the freshwater rotifer, B. calyciflorus, and the efficiency of mass production of resting eggs by this rotifer was similar to that of the seawater rotifers, B. plicatilis and B. rotundiformis.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.354-361
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• 2009

This study was carried out to develop an effective biocarrier-mediated bioaugmentation technology which will be useful for remediation of the crude oil-contaminated marine sediments. Enrichment of several microbial communities was made from several oil-polluted seashore sites and the two distinctively functional consortia have been successfully selected. These two consortia were grown together and used to manufacture the microbial agents for bioaugmentation of marine sediments polluted with crude oil. The most dominant species in the mixed culture was identified as Alcanivorax borkumensis based on pure culture and DGGE analysis. Bioaugmentation of oil-polluted marine sediments with the microbial agent MA-2 formulated using the mixed culture and biocarriers (activated carbon and minerals) was more effective, especially in combination with an oxygen producing (releasing) compound (ORC). Ninty percent of TPH was removed in the presence of ORC in 35 days while 74% in the absence of ORC. This indicated that the indigenous consortial degraders could be immobilized on the active carbon as a biocarrier to manufacture microbial agents and then effectively bioaugmented for remediation of the oil-polluted sediments.

• The Korean Journal of Mycology
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• v.22 no.3
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• pp.266-275
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• 1994

Brewer's yeast extract can be used as a good substrate for the culture of Lentinula edodes Mycelia(LEM). We found that it was better to filter the extract through three kinds of sieves and then to heat for hydrolysis and concentration at $90^{\circ}C$ prior to use. Also the maximum condition for the growth of LEM was investigated. We found that addition of inorganic salts such as calcium enhanced the growth of LEM. On the other hand, addition of carbon and nitrogen sources to the medium did not affect, and even inhibited under certain conditions, the growth of LEM. The maximum temperature for the growth of LEM was around $25^{\circ}C$. Also, it grows better when agitated by shaking at 100 rpm for airration. The appropriate concentration of the extract to use was 10%. Under these conditions, LEM could reach to the confluency after cultivation of 12 days. Our extract formula seems better than other available media for LEM growth, producing higher crude protein content and better taste.

• Proceedings of the Korea Database Society Conference
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• 1998.09a
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• pp.293-312
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• 1998

$\square$ 정보화 혁명의 대국민 인식수단과 초고속정보통신망의 주요 응용 서비스로서 부각 $\square$ 전통적인 정보이용방법에 대한 이용자들의 인식의 변화로 도서관 활성화 방안으로써 정보통신기술의 도입 $\square$ 선진 각국의 전자도서관 구축의 국가적 추진 $\square$ 국내 초고속정보통신망의 구축과 보급 확대 $\square$ 모든 국민이 지역, 시간의 제약없이 도서관에 접근하여 필요한 자료를 획득 $\square$ 정보획득시간 단축으로 국내연구자들의 연구력 증진 $\square$ 정보화의 지역격차 해소 $\square$ 국내 도서관의 전자도서관 사업 촉진 $\square$ 주요 전자도서관의 통합연동체제를 마련 $\square$ 주요 도서관별 대상분야 조정으로 중복투자 방지 $\square$ 초고속정보통신망의 주요한 응용서비스로 가시화 - 초고속정보통신망의 선도적 대국민 가시화 - 이에 따른 민간부문의 참여 및 투자 촉진 $\square$ 국가, 산업, 국민생활 정보화의 주요한 기반 구축 $\square$ 지역적 정보불균형 해소 - 일부지역에 편중되어 있는 정보를 인터넷을 이용하여 시.공간 제약없이 제공함으로써 정보의 불균형 해소 $\square$ 전자도서관의 기본모델 제시 -전자도서관의 주요기능인 타기관간 자료연동 및 검색시스템을 구현함으로써 향후 구축되는 전자도서관의 기본모델 제시 $\square$ 전자도서관간 자료공유체제 구축 -시범사업 참여도서관간 분산 관리하고 있는 정보의 공유를 위한 표준체제 구축(중략) 것으로 나타났다.까지 증가율을 보여 주었다. 것으로 나타났다.대표하는 압밀계수의 추정이 가능할 것으로 사료된다. $O_3$/라는 결정학적 관계를 가지며 에피탁샬 성장했음을 알 수 있었다.있었다.다(p<0.05)..8800-0.6800로 각각 표시되었다.~$\pm$10 V의 측정범위에서 memory window가 계속 증가하는 것을 보여주었다./$^{\circ}C$의 고주파 유전특성을 얻었다. 얻었다.끼쳤다.보였다.다. 싸이클링 성능을 보였다.다.보였다.다.고 입력 반사손실을 그림 I, 2, 3에 각각 나타내었다. 대책을 요구하고 있다.하는 경향을 보였다. 생존율은 48시간째부터 폐사하기 시작하여 144시간째에는 전량폐사하였다. 삼투압 조절 능력을 위한 여러가지 파라메타에서 15 $\textperthousand$구는 이상이 없는 것으로 추측되나, 0 $\textperthousand$구에서는 코티졸, Na$^{+}$, K$^{+}$, Cl￣, 총단백질 및 AST에서 시간경과에 따른 삼투압 조절 능력에 문제가 있는 것으로 보여진다.c}C$에서 5시간 가열조리 후 잔존율은 각각 84.7% 및 73.3%였고, 질소가스 통기하에서는 잔존율이 88.9% 및 81.8%로 더욱 안정하였다.8% 및 12.44%, 201일 이상의 경우 13.17% 및 11.30%로 201일 이상의 유기의 경우에만 대조구와 삭제 구간에 유의적인(p<0.05) 차이를 나타내었다.는 담수(淡水)에서 10%o의 해수(海水)로 이주된지 14일(日) 이후에 신장(腎臟)에서 수축된 것으로 나타났다. 30%o의 해수(海水)에 적응(適應)된 틸라피아의 평균 신사구체(腎絲球體)의 면적은 담수(淡水)에 적응된 개체의 면적보다 유의성

• Horticultural Science & Technology
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• v.33 no.1
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• pp.24-30
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• 2015

This study was conducted to select proper light intensity, pot media, and fertilization level for potted Hepatica asiatica Nakai native to Korea. The plants were grown under various light intensities (shading rate, 52, 82, 90, 97%) imposed by shading net. Plants grown with 52% shading showed a low survival rate (65%). Survival rate increased as shading increased, with over 80% survival in shading above 90%. Growth indexes such as fresh weight and leaf number did not show any significant difference between shading treatments. Plants grown in a soil mixture of decomposed granite:fertilizer-amended media:Kanumatsuchi (60:10:30, v/v/v) or river sand:fertilizer-amended media:bark (50:20:30) showed over 85% survival. However, plants grown in a soil mixture of river sand:fertilizer-amended media:Kanumatsuchi (50:30:20) or upland:river sand (40:60) showed very low survival, below 60%. Leaf number and plant height were the highest in a soil mixture of decomposed granite:fertilizer-amended media:Kanumatsuchi (60:10:30) as well. To select a proper fertilization level for H. asiatica, hyponex solution diluted 1,000- or 2,000-fold were applied weekly or biweekly. The survival rate was lowest at weekly application with 1,000-fold diluted solution, and no significant difference was observed between other treatments. In conclusion, H. asiatica exhibits preferences for very low light intensity and soil with air permeability, and is adaptable to a broad range of fertilization levels.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.3
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• pp.402-408
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• 2016

Master packaging system consists of an inner individual package and secondary outer package. During the stages of chilled transport and distribution, the combination of primary individual package and secondary package was used to maintain a modified atmosphere for shiitake mushrooms. During the retail stage at higher temperature ($25^{\circ}C$), the primary individual package was exposed to display conditions after dismantling of the secondary packaging. The master packaging system was constructed to contain eight individual $30-{\mu}m$ thick polypropylene film bags of 500 g shiitake mushrooms inside a $40-{\mu}m$ low-density polyethylene bag. Carbon dioxide absorbent material [$Ca(OH)_2$] and/or moisture absorbent (superabsorbent polymer) were designed in their required amounts based on respiration characteristics and then applied to the outer secondary packaging in sachet form. Gas concentration of the packaging, temperature, and humidity were monitored throughout transport and storage. The quality of shiitake mushrooms was measured at the retail stage to determine the packaging effect. During the distribution stage of 108 h, $O_2$ and $CO_2$ concentrations in the master packaging system were maintained at 9~11% and 1~4% in the inner packaging, respectively, which are good for quality preservation. Compared to the control, the master packaging with $CO_2$ and/or moisture absorbents improved mushroom preservation and particularly reduced decay.

• Journal of agriculture & life science
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• v.46 no.1
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• pp.163-176
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• 2012

TTo increase productivity of a strong extracellular alkaline protease (BCAP), stable strains of Bacillus clausii I-52 carrying another copy of BCAP gene in the chromosome were developed. Integrative vector, pHPS9-fuBCAP carrying BCAP promoter, ribosome binding site, signal sequence and active protease gene was constructed and transferred into B. clausii I-52, and integration of the constructed plasmid into chromosome was identified by PCR. An investigation was carried out on BCAP production by B. clausii I-52 and transformant C5 showing the highest relative activity of alkaline protease using submerged fermentation. Maximum enzyme activity was produced when cells were grown under the submerged fermentation conditions at $37^{\circ}C$ for 48 h with an aeration rate of 1 vvm and agitation rate of 650 rpm in a optimized medium (soybean meal 2%, wheat flour 1%, sodium citrate 0.5%, $K_2HPO_4$ 0.4%, $Na_2HPO_4$ 0.1%, NaCl 0.4%, $MgSO_47H_2O$ 0.01%, $FeSO_47H_2O$ 0.05%, liquid maltose 2.5%, $Na_2CO_3$ 0.6%). A protease yield of approximately 134,670U/ml was achieved using an optimized media, which show an increase of approximately 1.6-fold compared to that of non-transformant (83,960 U/ml). When the stability of transformant C5 was examined, the integrated plasmid pHPS9-fuBCAP was detected in the transformant after cultivation for 8 days, suggesting that it maintained stably in the chromosomal DNA of transformant C5.

• The Journal of Natural Sciences
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• v.11 no.1
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• pp.131-135
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• 1999

The objective of this research was to improve moisture retention capacity of pine bark. To achieve this, barks were ground with Wiley mill of hammer mill and were blended with recycled rockwool. Then, changes of soil physical properties were determined. The percentage of particles larger than 5.6 mm was 86.5% in raw materials. The percentage of particles larger than 1 mm decreased and those of particles smaller than 1 mm increased by grinding with Wiley mill or hammer mill. Grinding with Wiley mill showed better effect than those of hammer mill in decreasing particle size distribution. Grinding resulted in decreased total porosity (TP) and air space (AS) and increased container capacity (CC) and residual water content (RW), indication improved moisture retention capacity. The material ground with Wiley mill, than blended with 50% recycled rockwool had 81.1%, 67.7%, 13.5% and 235 ml in TP, CC, AS and RW, respectively. These results indicated that moisture retention capacity was improved by blending with recycled rockwood, but aeration of root media was much better than those of peat+vermiculite(1:1, v/v), which is commonly used in commercial production.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.136-144
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• 2009

Germination tests on 3 species that have potential for use as sprout vegetables, such as Coreopsis tinctoria, Saussurea pulchella, and Matricaria recutica, were conducted for 20 days under different temperatures of $15{\sim}30^{\circ}C$, and conditions of light and dark. C. tinctoria showed germination of 83% under $15^{\circ}C$ and dark condition after 4 days, S. pulchella 51.7% under $25^{\circ}C$ and light condition after 20 days, and M. recutica 90.3% under $25^{\circ}C$ and dark condition after 4 days. To investigate optimum plantlet size before greening treatment, seeds germinated were allowed to grow under darkness. The optimum growth of C. tinctoria was obtained under $30^{\circ}C$ after 5 days, S. pulchella under $25^{\circ}C$ after 6 days, and M. recutica under $20^{\circ}C$ after 6 days. Greening treatment resulted in diminished longitudinal growth, but C. tinctoria and S. pulchella showed more vigorous latitudinal growth. Days required before marketing as sprout vegetables were different according to species - Three days of greening was good for C. tinctoria, 2 for S. pulchella, no greening for M. recutica. Generally, tightly sealed containers for minimum water loss were recommended for storage of sprout vegetables after harvest. However, storage methods for each species were different depending on various factors like temperature of storage, presence of ventilation holes of storage packages and forms of marketing. More detailed research for above 3 species is proposed.