• Korean Journal of Microbiology
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• v.34 no.3
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• pp.144-148
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• 1998

Soil microorganisms including bacteria, fungi, and actinomycetes were seasonally isolated at depths (0.5~2, $10{\pm}1$, $50{\pm}1cm$) of field. The frequency of microbial isolates was employed for the determination of microbial population (CFU/g dry soil) and distribution ratio (%) in soil. Both bacteria (24-fold) and actinomycetes (7-fold) exhibited the biggest change at the depth of $50{\pm}1cm$, whereas fungi showed the maximum (13-fold) at $10{\pm}1cm$. On the whole, the bacterial population was high in spring soil, fungi in winter, and actinomycetes in autumn. Soil microorganisms also exhibited the seasonal variation on their distribution ratio (%). The maximum distribution ratio (85.7%) of bacteria was observed at the depth of $50{\pm}1cm$ in spring, whereas bacteria showed the minimum (35.2%) at the depth of $10{\pm}1cm$ in spring. The maximum distribution ratio (23.0%) of fungi was found at the depth of $50{\pm}1cm$ in spring, whereas its minimum (0.5%) at the depth of $10{\pm}1cm$ in spring. Actinomycetes exhibited the maximum distribution ratio (45.2%) at the depth of $10{\pm}1cm$ in spring, whereas its minimum (12.2%) was showed at the depth of $50{\pm}1cm$ in spring.

• Microbiology and Biotechnology Letters
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• v.41 no.2
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• pp.215-220
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• 2013

Biofilters for the removal of methane using tobermolite, perlite and polyurethane as packing materials have been undergoing recent development. The effects of these packing materials on methane oxidation activity were evaluated in this study. Mixed methanotrophs (consortia A, B, C and D) from wetland and landfill soils were used as the inoculum sources. The influences of packing materials, consisting of tobermolite, perlite, and polyurethane, on the methane oxidation rate and methanotrophic bio-mass, were estimated. When perlite was added into the methanotrophic cultures, the methane oxidation rate was more than twice that of the control (without packing materials), and the methanotrophic biomass increased more than 10 fold. The ratio of methanotrophic bacteria to total bacteria under with tobermolite packing material was higher than the control and the other packing materials, indicating that tobermolite can serve as a specific packing material where dominance of methanotrophs is desired. Therefore, perlite and tobermolite provide habitats which increase the activity of methanotrophic bacteria, and these packing materials are promising for use in methane oxidation processes.

• 보존과학연구
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• s.37
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• pp.5-15
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• 2016

We isolated microorganisms from soil arounding the stump and surface of stump in Bubjusa temple in Boeun, Heungguksa temple in Yeosu and Leeyongwoo house in Boseong. As a result, a distribution percentage of bacteria was high and there was a region-specific difference in bacteria colony. There was greater microbial diversity and population in the Leeyongwoo house in Boseong.

• The Korean Journal of Mycology
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• v.13 no.4
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• pp.195-201
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• 1985

Numbers of total bacteria and fungal propagules were highly populated in the soil of diseased plot in Gumsan, whereas numbers of actinomycetes were high in the soil of healthy plot in Goesan. In microbial fluctuation of different soil conditions four weeks after amended with various plant materials, bacteria were decreased in the soil amended with various plant materials as compared with non-amended soil except for Goesan. On the contrary, numbers of fungal propagules were increased amended with onion and potato stalk in two soils at different conditions, and also the propagules were enhanced in the soil amended with various plant materials in general. Higher numbers of bacteria and fungal propagules were observed in the diseased replanted plot in general, but germination rate of fungal spores relatively reversed against the microbial population. Alternaria panax was not affected much in their germination by natural soil condition, while Fusarium spp. were affected in their germination by natural soil. Natural soil showed a fungistatic effect against four fungal spores.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.6
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• pp.386-391
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• 2006

This study was conducted to evaluate the indole acetic acid (IAA) formation in soils as a biological indicator for the health of rice paddy soils with control, nitrogen sole, chemical fertilizer (NPK), and chemical fertilizer plus compost (CNPK) plots. There was a positive relationship between colorimetric method and high performance liquid chromatography for IAA in soils determined, and the values were similar between two methods, as $0.83{\sim}1.23{\mu}g\;IAA\;g^{-1}h^{-1}$ in colorimetric method, $0.80{\sim}1.29{\mu}g\;IAA\;g^{-1}h^{-1}$ in HPLC method. Numbers of dehydrogenase-producing bacteria and the IAA production in soils were high in NPK and CNPK plots comparing with control and nitrogen sole plots. Also there was high correlation between numbers of dehydrogenase-producing bacteria and IAA production in soils.

• Korean Journal of Heritage: History & Science
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• v.47 no.4
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• pp.92-105
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• 2014

Microbial characteristics of bacterial population were investigated in human remains and soil inside the bones in excavated grave no.4 and no.5 at Keundokgol site, Osu-ri, Buyeo. Phylogenetic characteristics of bacterial populations were analyzed by direct extracting of ancient DNA. In this study, based on the 16S rDNA sequences, in case of grave no.4, 319s from human remain were classified into 11 phyla, and 462s from soil were classified into 16 phyla. In case of grave no.5, 271s from human remain were classified into 10 phyla, and 497s from soil were classified into 11 phyla. Especially, Actinobacteria phylogenetic group are dominant group of bacterial populations in grave no.4 and no.5. Also, most of these were analyzed uncultured group. Thus, the discovery of a diversely microbial community and uncultured group was thought to be due to the specificity of the sample. Conclusively the general excavated human bones were contaminated with soil bacteria species their near around. This results contribute to preservation and management of ancient human bone from archaeological sites.

• Korean Journal of Microbiology
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• v.39 no.4
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• pp.260-266
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• 2003

In this study bacterial community was analyzed to evaluate the impacts of long-term use of agricultural chemicals on a soil ecosystem as well as to obtain fundamental data on the relationship. Sequences of 16S rRNA clones from a non-agricultural site and a tangerine orchard soil which has a history of long-term use of agricultural chemicals over 30 years were analyzed. This revealed that bacterial community containing 5 divisions and 18 genera was distributed in a tangerine orchard soil, while bacterial community containing 9 divisions and 44 genera was distributed. In a tangerine orchard soil site, the most abundant bacteria in subdivision level were placed into Proteobacteria γ group which occupied 56% of total clones. The other bacterial clones from the ocrhcard soil exposed to agricultural chemicals over 30 years were Acidobacteria group (25%), Fimicutes group (5%), Planctomycetes group (2%), Proteobacteria α (1%), δ group (1%), and Cyanobacteria group (1%). Whereas, the clones were from the non-agricultural site were distributed among the division or subdivision Acidobacteria group (14%), Planctomycetes group (13%), Proteobacteria α (10%), β (9%), δ (9%), Fimicutes group (8%), Verrucomicrobia group (8%), Actinobacteria group (6%), Proteobacteria γ group (3%), Bacteroidetes group (3%), Gemmatimonadetes group (3%), and Cyanobacteria group (1%). This finding suggests the possibility that long-term application of agricultural chemicals or fertilizers on a tangerine orchard might result in drastic reduction or alteration in the composition of the bacterial community in the contaminated soil site.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.236-241
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• 2011

Soil microbial diversity was responsible for a strong effect on the chemical properties of orchard soils. This study evaluated a relationship between soil chemical properties and soil microbial diversities at 25 sites in orchard soils in Gyeongnam Province. The average nutrients in the orchard soils were 2.6 times for available phosphorous, 2.3 times for exchangeable potassium and 1.3 times for exchangeable calcium higher compared to recommend concentrations in the orchard soils. Contents of available phosphorous and organic matter in the inclined piedmont soils were higher than those in the other topographical soils (p<0.05). Populations of fungi and fluorescence Pseudomonas sp. in the silt loam soils were significantly higher than those in the sandy loam soils (p<0.05). In principal component analysis of chemical properties and microbial populations in the upland soils, our findings suggested that population of bacteria should be considered as potential factor responsible for the clear orchard soils differentiation. The soil organic matter was significantly negative correlation with population of bacteria whereas was positive correlation with population of fungi in orchard soils.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.222-223
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• 2001

Arylsulfatase (EC 3.1.6.1)는 arylsulfate의 황산 ester 결합을 비가역적으로 가수분해하는 효소이다. 이러한 효소는 토양에서 처음 발견된 이래 동식물 및 미생물에서 검출되어졌으며, 주로 토양 중 황화합물의 recycle에 관여하여 토양 환경에 영향을 미치는 요인으로 보고되었다 (Spei. and Ross, 1978). Arylsulfatase에 대한 연구는 주로 토양으로부터 분리된 세균에 국한된 실정이다. (중략)

• Korean Journal Plant Pathology
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• v.1 no.1
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• pp.17-21
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• 1985

Microbial antagonism between virulent and avirulent isolates of Pseudomonas solanacearum was studied in relation to the control of bacterial wilt of tobacco. In nutrient broth media or in soil, the avirulent isolate of P. solanacearum grew faster than did the virulent one. Inhibitory effect of avirulent isolate against growth of virulent one was negligible in mixed culture of the two isolates. The disease severity of bacterial wilt was significantly reduced when the roots of cultivar BY 4 susceptible to bacterial wilt was dipped in suspension of an avirulent isolate for 6 hours prior to transplanting to the soil infested with virulent bacteria. When the seedlings of tobacco were poured with the suspension of an avirulent isolate onto the soil in pre-planting pots 24 hours before ransplanting, there was a significant reduction in disease severity in the field. However, the reduction was noticed until early July, but after middle of July, no difference between the avirulent isolate-treated and non-treated plants was found in severity of the bacterial wilt.