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Anti-oxidative and Immunomodulating Activities of Solvent Extracts from Broccoli (Brassica oleracea) Sprouts (브로콜리 새싹 용매 추출물의 항산화 및 면역조절 활성)

  • Koh, Jong-Ho;Kim, Hoon;Hwang, Jong-Hyun;Yu, Kwang-Won
    • The Korean Journal of Food And Nutrition
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    • v.32 no.1
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    • pp.1-10
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    • 2019
  • In order to examine the functionality of broccoli sprout (Brassica oleracea, BS), solvent extracts were prepared and their anti-oxidative and immunomodulating activities were compared with those of broccoli (B). EtOH extracts (E) were potently higher than hot-water extracts (HW) in the antioxidant contents and radical scavenging activity. In particular, the total polyphenolic contents in addition to ABTS and DPPH radical scavenging activity were significantly higher in EtOH extract of broccoli sprout (BS-E; 9.15 mg GAE/g, 4.52 mg AEAC/g, and 1.14 mg AEAC/g) compared with that of broccoli (B-E; 7.83 mg GAE/g, 3.63 mg AEAC/g, and 0.97 mg/AEAC/g). Whereas, total flavonoid content was significantly higher in B-E (1.60 mg QE/g) than BS-E (1.43 mg QE/g). Anti-inflammatory activity was investigated using LPS-induced cell line model at a concentration of $10{\sim}100{\mu}g/mL$, in which all solvent extracts of both broccoli sprouts and broccoli were not toxic to RAW 264.7 cell lines. In anti-inflammatory activity of broccoli sprouts, EtOH extracts also showed significantly more potent activity than hot-water extracts in all sample concentrations tested. In addition, BS-E ($100{\mu}g/mL$) inhibited nitric oxide (NO) and IL-6 production to 60.9% and 68.9% compared with the LPS inflammation group (without extracts), whereas B-E inhibited 49.6% and 54.9%. On the other hand, in immunostimulating activity by splenocytes and macrophages, hot-water extract showed significantly higher activity than EtOH extract. Especially, BS-HW stimulated the splenocyte proliferation (1.2-fold against saline group) and IFN-${\gamma}$ production (264.39 pg/mL) at $100{\mu}g/mL$, and the production of IL-6 (1.33-fold), IL-12 (1.09-fold) and TNF-${\alpha}$ (1.49-fold) from macrophages was also significantly enhanced over broccoli. In conclusion, broccoli sprouts showed more potent anti-oxidative, anti-inflammatory and immunostimulating activity than broccoli, suggesting the possibility of using broccoli sprouts as functional food materials.

Evaluation of Mechanical Test Characteristics according to Welding Position in FCAW Heterojunction (FCAW 이종접합에서 용접자세에 따른 기계적 시험 특성 평가)

  • Cho, Byung-Jun;Lee, Soung-Jun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.8
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    • pp.649-656
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    • 2019
  • Flux cored arc welding (FCAW), which is used widely in many fields, such as shipyards, bridge structures, construction machinery, and plant industry, is an alternative to shielded metal arc welding (SMAW). FCAW is used largely in the welding of carbon and alloy steel because it can be welded in all poses and obtain excellent quality in the field under a range of working conditions. In this study, the mechanical properties of welded parts were analyzed after different welding of SS400 and SM490A using FCAW. The following conclusions were drawn. The tensile test results satisfied the KS standard tensile strength in the range of 400~510 N/mm2 in all welding positions. The bending test confirmed that most of the specimens did not show surface breakage or other defects during bending and exhibited sufficient toughness, even after plastic deformation. The hardness test results were lower than the standard value of 350 Hv of KS B 0893. Similar to the hardness test, were greater than the KS reference value. The macro test revealed no internal flaws, non-metallic inclusions, bubbles or impurities on the entire cross section of the weld, and there were no concerns regarding lamination.

Comparison of Virus Elimination Methods for Disease-free Seedlings of the Apple Dwarfing Rootstock (사과 왜성대목 무독묘 생산을 위한 바이러스 제거 방법 비교)

  • Kwon, Young Hee;Lee, Joung Kwan;Kim, Hee Kyu;Kim, Kyung Ok;Park, Jae Seong;Huh, Yoon Sun;Yoon, Yeo Joong
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.54-54
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    • 2019
  • Apple (Malus domestica) is one of the most economically important fruits in Korea. But virus infection has decreased sustainable production of apple and caused the serious problems such as yield loss and poor fruit quality. Virus or viroid infection including Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV) and Apple scar skin viroid (ASSVd) has been also reported in Korea. In many cases, apple is infected with virus and viroid with no specific symptoms, the damage caused by the virus are unaware significantly. In our research, we tried to eliminate viruses in the rootstock for the disease-free seedlings of the apple dwarfing rootstock M.9 and M.26. The method of virus elimination was meristem culture, heat($37^{\circ}C$, 6weeks) treatment and chemistry($Ribavirin^{(R)}$) treatment. The analytical methods commonly used for the detection of virus is Enzyme-linked Immuno-Sorbent Assay(ELlSA) and Reverse Transcription-polymerase Chain Reaction(RT-PCR). RT-PCR method was more 30% sensitive than ELISA method. Efficiency of method eliminate virus appeared meristem method > heat treatment > chemistry treatment. The higher acquisition rate of disease-free seedlings is 30~40% on meristem treatment. In meristem treatment, the apple dwarfing rootstock M.9 gained infection ratio of ACLSV, ASPV and ASGV were 45%, 60% and 50% respectively. In the apple dwarfing rootstock M.26, infection ratio of ACLSV, ASPV and ASGV were 40%, 55%, 55%, respectively. Based on our results, it was found that most effective method of disease-free seedlings apple dwarfing rootstocks was by meristem treatment than heat method and chemistry treatment.

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Effect of Persimmon Peel as an Additional Feeding (사료에 첨가된 감껍질의 효과)

  • Shin, Sangsu;Yi, Junkoo;Kong, Changsu;Kwon, Woosung;Ryoo, Zaeyoung;Kim, Eungyung;Kim, Hyeonjin;Ha, Jaejung;Hong, Doyoung;Yoon, Duhak;Kim, Sunghyun;Kim, Myoung Ok
    • Korean Journal of Poultry Science
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    • v.46 no.2
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    • pp.87-94
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    • 2019
  • Huge amount of persimmon peels, which are byproducts usually arising after making dried persimmons from fresh persimmons, are generated in fall, every year. As the dry matter of persimmon peel contains carbohydrates over 80%, it could be a good candidate for feed. In this study, we evaluated the persimmon peel as a feed resource for a hen laying eggs. Persimmon peel has lower ratio of crude protein and fat composition while higher ratio of crude fiber composition compared to a basal laying hen feed. Feeding the persimmon peel by adding to the feed at the level of 2% or 5% did not affect the egg-laying rate from beginning to the peak of laying. In addition, feeding the persimmon peel did not affect the egg quality either, as the pH, Haugh unit, yolk color and shell thickness of the egg were not different from each other. The composition of amino acids and fatty acids were also almost same for each other, except for the linolenic acid which was slightly higher in the persimmon peel feeding groups. In conclusion, addition of persimmon peel to the feed up to 5% might be helpful to increase the income and reduce the waste produced from the agricultural byproduct.

Occurrence of Gray Mold Caused by Botrytis cinerea on Okra in Korea (Botrytis cinerea에 의한 오크라 잿빛곰팡이병)

  • Choi, JangNam;Choi, InYoung;Lee, KuiJae;Lee, JungNo;Cho, SeongWan;Shin, HyeonDong;Galea, Victor
    • Research in Plant Disease
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    • v.24 no.4
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    • pp.302-307
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    • 2018
  • From 2014 to 2016, approximately 5% of okra fruit were observed displaying gray mold symptoms at the research field of Jeollabuk-do Agricultural Research and Extension Services, Korea. The symptoms observed were water-soaked, brown or gray spots, and abundant mycelial with conidia appearing on the infected fruit. Initial infection commenced from the base of fruit and gradually moved to the pod, where it finally resulted in collapse. Colonies on potato dextrose agar were gray to grayish brown, felted and cottony expanding 65-80 mm after one week. The fungus formed several black sclerotia ranging $1.0-3.5{\times}0.5-3.0mm$ on the Petri dish after two weeks. The conidia were one-celled, ellipsoidal or ovoid, colorless or pale brown, and $6.2-15.4{\times}5.0-10.4{\mu}m$. Conidiophores arose solitary or in groups, straight or flexuous, septate, with an inflated basal cell brown to light brown, and measured $85-450{\times}10.0-40.0{\mu}m$. On the basis of the morphological characteristics and phylogenetic analyses of internal transcribed spacer rDNA, the fungus was identified as Botrytis cinerea Pers. Pathogenicity of a representative isolate was proved by artificial inoculation, fulfilling Koch's postulates. To our knowledge, this is the first report on the occurrence of B. cinerea on okra in Korea.

A Business Model for Offshore Integrated Drilling Commissioning in Korea and Related Economic Analysis (우리나라 해양시추설비 통합시운전 사업 모델의 개발 및 경제성 분석에 관한 연구)

  • Lee, Chang-Woo;Cheon, Young-Wook;Shin, Sang-Hoon;Shin, Yong-John
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.1
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    • pp.102-110
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    • 2019
  • The shipbuilding and offshore plant industry of Korea is important and leads Korea's economic growth, designated as the 1st to 4th export items in Korea in terms of export contribution over the period from 2011 to 2015. This study proposes ways to improve the national competitiveness of Korean shipyards in the global offshore drilling market by reviewing a business model for providing an integrated offshore drilling commissioning service in Korea. This commissioning service model, which was attempted in 2014, was reviewed, and a new proposed business model for overcoming the limitations of the previous model and activating further business was evaluated. As a result of an economic evaluation, it was found that a 150-meter water depth model is economically more effective. As the number of integrated commissions increased from 2 to 5 times per year, NPV, IRR and B/C ratios increased and the fee per use decreased. Therefore, for offshore drilling facilities constructed and delivered in Korea, it will be necessary to encourage integrated offshore commissioning.

Analysis of Correlation between Marine Traffic Congestion and Waterway Risk based on IWRAP Mk2 (해상교통혼잡도와 IWRAP Mk2 기반의 항로 위험도 연관성 분석에 관한 연구)

  • Lee, Euijong;Lee, Yun-sok
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.25 no.5
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    • pp.527-534
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    • 2019
  • Several types of mathematical analysis methods are used for port waterway risk assessment based on marine traffic volume. In Korea, a marine traffic congestion model that standardizes the size of the vessels passing through the port waterway is applied to evaluate the risk of the waterway. For example, when marine traffic congestion is high, risk situations such as collisions are likely to occur. However, a scientific review is required to determine if there is a correlation between high density of maritime traffic and a high risk of waterway incidents. In this study, IWRAP Mk2(IALA official recommendation evaluation model) and a marine traffic congestion model were used to analyze the correlation between port waterway risk and marine traffic congestion in the same area. As a result, the linear function of R2 was calculated as 0.943 and it was determined to be significant. The Pearson correlation coefficient was calculated as 0.971, indicating a strong positive correlation. It was confirmed that the port waterway risk and the marine traffic congestion have a strong correlation due to the influence of the common input variables of each model. It is expected that these results will be used in the development of advanced models for the prediction of port waterway risk assessment.

Design and Implementation of Fruit harvest time Predicting System based on Machine Learning (머신러닝 적용 과일 수확시기 예측시스템 설계 및 구현)

  • Oh, Jung Won;Kim, Hangkon;Kim, Il-Tae
    • Smart Media Journal
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    • v.8 no.1
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    • pp.74-81
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    • 2019
  • Recently, machine learning technology has had a significant impact on society, particularly in the medical, manufacturing, marketing, finance, broadcasting, and agricultural aspects of human lives. In this paper, we study how to apply machine learning techniques to foods, which have the greatest influence on the human survival. In the field of Smart Farm, which integrates the Internet of Things (IoT) technology into agriculture, we focus on optimizing the crop growth environment by monitoring the growth environment in real time. KT Smart Farm Solution 2.0 has adopted machine learning to optimize temperature and humidity in the greenhouse. Most existing smart farm businesses mainly focus on controlling the growth environment and improving productivity. On the other hand, in this study, we are studying how to apply machine learning with respect to harvest time so that we will be able to harvest fruits of the highest quality and ship them at an excellent cost. In order to apply machine learning techniques to the field of smart farms, it is important to acquire abundant voluminous data. Therefore, to apply accurate machine learning technology, it is necessary to continuously collect large data. Therefore, the color, value, internal temperature, and moisture of greenhouse-grown fruits are collected and secured in real time using color, weight, and temperature/humidity sensors. The proposed FPSML provides an architecture that can be used repeatedly for a similar fruit crop. It allows for a more accurate harvest time as massive data is accumulated continuously.

Characteristics of Filtration Treatment Using Diatomite Filter Aids for Sewage Water Reuse (하수처리수 재이용을 위한 가압식 규조토 여과의 처리성능평가)

  • Lim, Byung Ran;Kim, Hee Seo;Go, Yeon Sil;Kim, Hyun Kab;Kim, Jong Hak;Lee, Tae Jin
    • Journal of Korean Society on Water Environment
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    • v.35 no.2
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    • pp.145-151
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    • 2019
  • The purpose of this study was to investigate treatment characteristics of diatomite filtration, that would allow water recovery from biologically-treated effluent for reuse. Diatomite, Celpure 100, and acid clay were used as filter-aids, with a support filter manufactured from polyethylene (PE), and polypropylene (PP). This pre-coating process using diatomite filter-aids, is used in the filtration range of pressure filters, and has consistently provided high-quality separation. The results showed that variations in average removal efficiency of SS, and T-P from biologically treated effluent by the diatomite-coated PE filter, were approximately 82.2 ~ 88.9 % and 4.8 ~ 21.1 %, respectively. T-P treatment efficiency of the PP filter pre-coated with diatomite and $Celpure^{(R)}100$ at $57.64g/m^2$, was approximately $24{\pm}10%$ and $40{\pm}15%$ on average, respectively. Particle size distribution of secondary effluent varied from 0.05 to $200{\mu}m$, and $d_{50}$ value was $20.76{\mu}m$. The size distribution of particles in the diatomite filtrate ranged from 1.26 to $101.1{\mu}m$ when pre-coated with diatomite filter-aid, at a content of $57.64g/m^2$. Diatomite filter aids, i.e., the particles that form the pre-coating layer, capture very fine particles as well as macromolecules, owing to their complex structure with numerous fine microscopic pores, and surface properties. The filtration process using diatomite and $Celpure^{(R)}100$ as filter aids, has been successfully applied, to recover water from sewage for reuse. The disadvantage of the process, is that the particle size of the filter-aid is spent, because of pressurization.

A Qualitative Study on English Speaking Tasks Experienced by Beginner Level EFL Learners (초급 수준의 영어학습자들이 경험한 그림을 활용한 영어 말하기 과업에 관한 연구)

  • Kim, Byung-Sun;Yoon, Tecnam
    • The Journal of the Korea Contents Association
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    • v.21 no.10
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    • pp.603-612
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    • 2021
  • The purpose of this study is to allow beginner level English learners to experience the English speaking task using pictures, and to analyze the meanings of the experience using a phenomenological research method. As research participants, 10 freshmen majoring in Power Generation Facilities at Korean Polytechnic University in Gangwon-do were selected. Face-to-face interviews and SNS were used for data collection, and Colaizzi's research method was adopted for data analysis. As a result of the analysis, 9 themes, 4 theme clusters, and 2 categories were derived. The results are as follows. First, the participants were able to find hope that they could speak English at their own level through the English speaking task using pictures. Second, they stated that the effect of the visual medium of painting increased concentration and curiosity and lowered anxiety. Third, it was recognized that self-confidence, a speaker like a native speaker, and quickness of speaking improved due to familiarity with speaking English. Fourth, the biggest difficulty in the English speaking task was vocabulary. So, they felt the limitation in explaining the picture, and they were having a lot of trouble in translating Korean words into English words. Finally, through the results of this study, the effect of the medium of picture was confirmed, and necessary future studies were suggested.