• Journal of Life Science
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• v.27 no.12
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• pp.1410-1414
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• 2017

Escherichia coli tryptophan synthase (TS) contains ${\alpha}_2{\beta}_2$, which catalyzes the final two steps in Trp biosynthesis. A molecular tunnel exists between the two active sites of ${\alpha}$ and ${\beta}$ subunits in TS. Via intersubunit communication, TS increases catalytic efficiency, including substrate channeling. The ${\beta}$ subunit of TS is composed of two domains, one of which, the COMM (communication) domain, plays an important role in intersubunit communication. The ${\alpha}$ subunit has a TIM barrel structure. This protein has functional regions at the C terminal of ${\beta}$ pleated sheets and in its loop regions. Three regions of the ${\alpha}$ subunit (${\alpha}L6$ [${\alpha}-loop$ L6], ${\alpha}L2$, and ${\alpha}L3$) are implicated in intersubunit communication. In the present study, conformational changes in ${\alpha}L6$ were monitored by measuring the sensitivity of mutant proteins in these regions to trypsin. The addition of a ${\alpha}$ subunit-specific ligand, D,L-${\alpha}$-glycerophosphate (GP), partially restored the sensitivity of mutant proteins to trypsin. In contrast, the addition of the ${\beta}$ subunit-specific ligand L-serine (Ser) resulted in varied sensitivity to trypsin, with an increase in PT53 (substitution of Pro with Thr at residue 53) and DG56, decrease in NS104 and wild type, and no change in GD51 and PH53. This finding may be related to several reaction intermediates formed under this condition. The addition of both GP and Ser led to a highly stable state of the complex. The present results are consistent with the current model. The method used herein may be useful for screening residues involved in intersubunit communication.

• Journal of the Korean Arthroscopy Society
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• v.16 no.2
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• pp.160-166
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• 2012

Purpose: The aim of this study was to determine the patterns of the stress distribution within the reconstructed anterior cruciate ligament (ACL) double bundles in response to an anterior tibial load and rotatory load at $45^{\circ}$ flexed knee model by use of a 3-dimensional finite element analysis (FEM). Materials and Methods: The $0^{\circ}$ and $45^{\circ}$ flexed 3-D knee model were reconstructed based on the high resolution computed tomography (CT) images from the right knee of a healthy male subject. To simulate double bundle ACL reconstruction, in $0^{\circ}$ analytic model, four 7 mm diameter tunnels were created at the center of each anteromedial (AM) and posterolateral (PL) footprints on the femur and tibia. The grafts were inserted into the corresponding bone tunnels and then reconstructed knee model was flexed to $45^{\circ}$. As a next step, the 5 mm anterior tibial load and internal rotational load of $10^{\circ}$ were applied on the final Computer aided design (CAD) model. And then stress patterns of each bundle were assessed using a finite element analysis. Results: In response to the 5 mm of anterior tibial load, the AM bundle showed increased stresses around the tibial and femoral attachment sites; especially in the anterior aspect of the bundle. In the PL bundle, the highest stress concentration was also noticed on the anterior aspect of the bundle. Under $10^{\circ}$ internal rotational load, the stress concentration was predominant around the anterior aspect of the tibial attachment site within the AM bundle. The PL bundle also showed highest stress concentration on the anterior aspect of the bundle. Conclusion: Although the stress patterns were not identical among the AM and PL bundle, there were common trends in the stress distribution. The stress concentration was predominant on the anterior aspect of both bundles in response to the anterior tibial load and rotatory load.

• Tunnel and Underground Space
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• v.25 no.5
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• pp.462-469
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• 2015

This study carried out a topographic survey at a small-scale open-pit limestone mine in Korea (the Daesung MDI Seoggyo office) using a popular rotary-wing unmanned aerial vehicle (UAV, Drone, DJI Phantom2 Vision+). 89 sheets of aerial photos could be obtained as a result of performing an automatic flight for 30 minutes under conditions of 100m altitude and 3m/s speed. A total of 34 million cloud points with X, Y, Z-coordinates was extracted from the aerial photos after data processing for correction and matching, then an orthomosaic image and digital surface model with 5m grid spacing could be generated. A comparison of the X, Y, Z-coordinates of 5 ground control points measured by differential global positioning system and those determined by UAV photogrammetry revealed that the root mean squared errors of X, Y, Z-coordinates were around 10cm. Therefore, it is expected that the popular rotary-wing UAV photogrammetry can be effectively utilized in small-scale open-pit mines as a technology that is able to replace or supplement existing topographic surveying equipments.

• Tunnel and Underground Space
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• v.26 no.5
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• pp.396-408
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• 2016

Rock fracture mechanics has been widely applied to various fields of rock and civil engineering. But most researches covered mode I behavior, though mode II behavior is dominant in rock engineering. Until now, there is only one ISRM suggested method for mode II toughness of rock. A new SCC (Short Core in Compression) mode II toughness test method was developed considering 1) application of confining pressure, 2) easiness of notch creation, 3) utilization of existing equipment, 4) simple test procedure. The stress intensity factors were determined by 3D finite element method considering line and distributed loading conditions. The tests with granite specimens were carried out using MTS 815 rock test system with a loading rate of 0.002 mm/s. The mean value of mode II fracture toughness of granite showed $2.33MPa{\sqrt{m}}$. Mode I toughness of the same granite was $1.12MPa{\sqrt{m}}$, determined by Brazilian disk test and $K_{IIC}/K_{IC}=2.08$. The smooth fracture surface with rock powder formation also supported mode II behavior of SCC method. The SCC method can be used for the determination of mode II fracture toughness of rocks based on the current results.

• Tunnel and Underground Space
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• v.24 no.2
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• pp.176-186
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• 2014

Roughness, aperture and filling material of rock joint are widely considered to affect the hydraulic characteristics of joint. Among these factors, in this study, the joint roughness was examined with artificial joint profiles generated by Monte Carlo simulating on the original profiles suggested by Barton and Choubey(1977). Original profiles and revised profiles were combined to establish flow channel models, in which the hydraulic characteristics were analyzed numerically on the basis of minimum aperture changes and flow channel shapes. Maximum flow rate was identified at the growing point of flow area after passing through minimum aperture generated by the two profiles, and it was resulted that maximum flow rate is inversely proportional to minimum aperture. Maximum flow rate per unit area showed different values because flow channel shapes and minimum aperture locations are different in each model. In flow channel, mechanical aperture showed approximately 1.07 ~ 3.00 times larger than hydraulic aperture. In this study, mechanical and hydraulic aperture were concluded to be closely related to $A_i$ value, and their relations can be denoted by $e_m=0.519A^{0.7169_i}$ and $e_h=0.6182A^{0.239}_i$, respectively.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.12
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• pp.73-80
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• 2012

In this article, we propose a P2P-based mobility management protocol for global seamless handover in heterogeneous wireless networks. Unlike previous mobility management protocols such as IETF MIPv4/6 and its variants, the proposed protocol can support global seamless handover without changing the existing network infrastructure. The idea of the proposed protocol is that the location management function for mobility management is separately supported from packet forwarding function, and bidirectional IP tunnels for packet transmission are dynamically constructed between two end-to-end mobile hosts. In addition, early handover techniques have been developed to avoid large handover delays and packet losses using the IEEE 802.21 Media Independent Handover functions. The architecture and signaling procedure of the proposed protocol have been designed in detail, and the mathematical analysis and simulation have been done for performance evaluation. The performance results show that the proposed protocol outperforms the existing MIPv6 and HMIPv6 in terms of handover latency and packet loss.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.48 no.12
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• pp.17-27
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• 2011

Most of the previous mobility management protocols such as IETF MIPv4/6 and its variants standardized by the IETF do not support global seamless handover because they require partially changes of the existing network infrastructure. In this article, we propose a simple mobility management protocol (SMMP) which can support global seamless handover between homogeneous or heterogeneous wireless networks. To do this, the SMMP employs separate location management function as DMMS to support global user and service mobility and the bidirectional tunnels are dynamically constructed to support seamless IP mobility by using the IEEE MIH extension server, which is extended the IEEE 802.21 MIH standards. The detailed architecture and functions of the SMMP have been designed. Finally, the mathematical analysis and the simulation have been done. The performance results show the proposed SMMP outperforms the existing MIPv6 and HMIPv6 in terms of handover latency, packet loss, pear signal noise ratio (PSNR).

• Tunnel and Underground Space
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• v.27 no.1
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• pp.26-38
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• 2017

In this study, bedding rock permeability was measured using Berea sandstones with three different beddings. The fracture permeability was also measured using tight sandstone with two different fracture regimes considering in-situ stress conditions. The Berea sandstone with vertical, horizontal and non-bedding was used to analyze evolution of permeability upon in-situ stress conditions. In order to describe applied effective stress around rock in underground, the triaxial pressure cell & hydrostatic pressure cell was designed and permeability experiments were performed with controlled axial and confining pressures. The measurement of permeability was conducted by increasing and decreasing effective stress. The permeability of non-bedding rock sample is the most sensitive to applied stress conditions and fracture permeability of tight sandstone increases with fracture treatment with proppant.

• Tunnel and Underground Space
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• v.30 no.5
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• pp.473-483
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• 2020

This paper examines the vertical stress change concentrated on mine pillar which occurs due to the stress disturbance from opening excavation at room and pillar mine by FLAC3D, a finite difference method (FDM) software. The mesh size combination is decided with a careful consideration of relative error and run-time, then its performance is verified. A series of numerical analyses is conducted and the vertical stress at central pillar was observed for the test cases of 1×1 to 11×11 mine pillars, 40 m to 320 m depth with 40 m difference. The results show that the vertical stress of pillar approaches to the similar value with the value estimated by tributary area theory(TAT) when the development area (N_P) is increased or the height of overburden (H_OB) is decreased, while it is overestimated in the opposite case. Furthermore, it also represents that the vertical stress factor (VSF) converges to a specific value when the depth is increased whille keeping the development area identical.

• Tunnel and Underground Space
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• v.24 no.1
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• pp.67-80
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• 2014

Long-term stability and delayed failure of granite were evaluated through the laboratory test based on Wilkins method and Brazilian disc test (BDT) which yields tensile strength, mode I fracture toughness and subcritical crack growth parameters. Then, the long-term strength of granite was estimated by using analytical models and long-term stability of compressed air-energy storage (CAES) pilot cavern pressurized up to 5 ~ 6 MPa was evaluated using numerical code, FRACOD with the determined subcritical crack growth parameters. The results of test and analyses showed that the subcritical crack growth index, n was determined as 29.39 and the inner pressure of 5 ~ 6 MPa had an insignificant effect on the long-term stability of pilot cavern. It was also found that the measurement and analysis of acoustic emission events can describe the accumulation of damage due to subcritical crack growth quantitatively. That is, AE monitoring can provide the current status of rock under loading if we make an identical installation condition in the field with that of the laboratory test.