• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.1
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• pp.276-280
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• 2011

This study investigated the activation conditions of pancreatic enzymes from porcine pancreas. Duodenum induced the activation of pancreatic protease and lipase in pancreas. When 10% duodenum was added to pancreatic juice and the mixture was incubated at $30^{\circ}C$ for 90 min or at $25^{\circ}C$ for 4 hrs, the activities of pancreatic protese and lipase reached the peak. When the pancreatin was prepared by sequential process of enzymatic activation at $25^{\circ}C$ for 4 hrs, centrifugation, acetone precipitation and freeze-drying, the specific activities of pancreatic protease, lipase and amylase were 136, 116 and 400 U/mg-protein, respectively. The protease, lipase and amylase activities of the prepared pancreatin were 5.4, 58.0 and 16.0 times higher than those of USP standard, respectively.

• The Korean Journal of Zoology
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• v.34 no.3
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• pp.420-425
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• 1991

본 연구는 인삼 사포닌(Ponax ginseng C.A. Meyer)이 수영운동을 부하시킨 웅성 횐쥐(Sprague-Dawley 계, 360 $\pm$ 40 9)의 대퇴근 크레아틴 키나제(Creative Kinase, CK: E.C. 2.7.3.2) 동위효소 활성에 미치는 영향을 연구하였다. 운동군은 3시간 동안 수영운동시켰으며 ,인삼군은 인삼 사포닌을 체중 Kg 당 120 mg 복강투여하였다. 인삼 사포닌을 투여한 운동군은 수영운동 1시간 전에 인삼 사포닌을 투여한 후 3시간 동안 수영시켰다. 인삼군의 MB-CK의 활성은 대조군보다 유의하게 증가하였다(P<0.01). 또한 인삼 비투여 운동군과 인삼 투여 운동군에서의 MM-CK활성은 대조군보다 각각 현저하게 증가하였고(P < 0.01와 P < 0.05), 특히 인삼 투여 운동군에서 BB-CK활성이 대조군보다 유의하게 증가하였다(P < 0.01). 인삼 사포닌은 일반적으로 CK-동위효소의 활성을 증가시키고, 운동은 MM-CK의 활성을 현저하게 증가시켰으며, 인삼 투여 운동군에서 BB-CK의 활성이 인삼 비투여 운동군보다 현저하게 증가하였다. 따라서 인삼 사포닌과 운동이 CK동위효소 활성에 상승의 효과를 나타내는 것으로 생각된다.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.1
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• pp.51-57
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• 2014

Purpose: The present study was conducted to establish the experimental condition for the proper evaluation of protein removal efficacy when developing protein removal agents. Its protein removal efficacy was further analyzed and compared with the result from protein removal efficacy against protein deposition on contact lens to suggest the evaluation method for efficacy of protein removal agents. Methods: Protein digestibility assay presented in the Korean pharmacopoeia was selected to establish the evaluation method for efficacy of papain, pancreatin, subtilisin A and protease itself as a ingredient and protein removal tablets or solution containing those enzymes and find a suitable test conditions. Furthermore, the cleaning efficacy of commercially available protein removal tablets and solution on balafilcon A lens deposited with protein artificially was measured and the correlation between two evaluation methods was further analyzed. Results: When pancreatin itself and the product containing pancreatin was evaluated by protein digestibility assay, both reached 28 IU/mg, the standard value of protein digestibility suggested by the Korean pharmacopoeia. In case of protease and subtilisin A tested with trichloroacetic acid B solution, both of them met the enzyme activity level proposed by the manufacturers when they were evaluated by protein digestibility assay however, papain and subtilisin A tested with trichloroacetic acid A solution were not reached the enzyme activity level. Among protein removal agents, three products except a product containing pancreatin did not meet the enzyme activity value specified by the manufacturer when they were evaluated by protein digestibility assay. However, actual protein removal efficacy of three products except a papain-containing product on the lens was greater than 90% protein removal. In the case of papain-containing protein removal product, its effect was not measured by protein digestibility assay however, its actual protein removal efficacy on the lens reached 73.72%. Conclusions: From the results, it was confirmed that the efficacy of protein removal agents for contact lens should be evaluated by different method according to the type of proteolytic enzyme contained. That is, the protein removal agents containing pancreatin, protease and subtilisin A can be evaluated by protein digestibility assay and protein removal efficiency evaluation and the products containing papain can be effectively evaluated by only the evaluation method for protein removal efficiency employing the lens.

• Journal of the Korean Electrochemical Society
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• v.15 no.4
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• pp.249-255
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• 2012

The planar-type amperometric creatinine biosensor employing an additional enzyme layer containing creatine kinase and adenosine triphosphate was developed to eliminate severe interference from creatine. In the additional enzyme layer, an interfering substance, creatine is converted to noninterfering product, phosphocreatine. Furthermore, the carbon electrode electroplated with Pt black(Pt-B) was employed to fabricate creatinine biosensors with improved sensor performance(e.g., sensitivity, reliability, and reproducibility). The creatinine levels in an unknown sample were determined within less than 5% errors using creatinine microsensors equipped in a flow-cell cartridge.

• KSBB Journal
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• v.18 no.4
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• pp.301-305
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• 2003

The study of pancreatin extraction was investigated by supercritical fluid process. Using supercritical carbon dioxide extraction with entrainer the purification of pancreatin was possible to remove lipids from Hog pancreas. To observe the optimum conditions different experimental variables were changed as pressure, temperature, flow rate of solvent and 0.25 mm of sample size were evaluated for effective removal of lipids. Ethanol and n-hexane were used as an entrainer with 5 mL/min. Increasing pressure at constant temperature the efficiency of the lipid removal in Hog pancreas was improved and the protein was concentrated without denaturalization, compared that of the control Hog pancreas. The most efficient conditions of lipid elimination were 17 MPa of pressure and 35$^{\circ}C$ of temperature and 0.25 mm of sample size.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.516-519
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• 2000

Generally pancreas consist of lipid, water and protein, digestion enzyme complex (pro-tease, lipase, amylase). The sample used in this work was frozen dry and treated by a semi-batch flow type. In order to develop a supercritical fluid extraction process to rem-ove lipid from the pancreas, experiments were conducted at various operating conditions(pressure range $1500{\sim}2800psi$, temperature range $25{\sim}40^{cdot}C$, particle size$(0.25{\sim}1.0mm$, flow rate $20{\sim}80m{\ell}/min)$. Also cholesterol in the pancreas was removed. The highest extraction efficiency was 2500psi, $35^{\cdot}C$, 0.25mm of pancreas size. The enzyme activity of the pancreas produced from this work showed high value compared with imported pancreas.

• The Journal of the Korea Contents Association
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• v.6 no.4
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• pp.136-145
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• 2006

The main purpose of the present study was to find the effect of short term creatine loading on repeated bouts of kicking, plasma creatine phosphokinase and anaerobic power in male taekwondo player from high school. Eighteen male were randomly assigned to two groups. They were tested before and after 6 days of placebo($4{\times}5$ glucose d-1, N=9) or Creatine monohydrate loading(consume 0.3g kg-1, N=9). Only creatine loading group were significantly increased of repeated bouts of kicking, plasma creatine phosphokinase and anaerobic power These results suggest that short creatine loading was effective diet protocol in taekwondo player from male high school.

• Korean Journal of Poultry Science
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• v.30 no.1
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• pp.55-59
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• 2003

A necrotizing skeletal myopathy was diagnosed in three flocks of 30,000 thirty-eight-week-old layer breeder chickens. The mortality attributed to the myopathy was 17.7%, 12.3% and 21.1% in flock A, B and C, respectively. Clinically, chickens were showed depression, anorexia, posterior paresis, inability to rise, incoordination, reluctance to move, and leg trembling and weakness. The most striking findings at necropsy was pale streaking in the muscles of the thighs and legs. Microscopic lesions included myofiber degeneration and necrosis with massive cellular proliferation interpreted as sarcolemmal nuclei proliferation. Plasma creatine kinase, asparte aminotransferase and akaline phosphatase were markedly elevated. In conclusion, author suggested that submitted chickens were affected by ionophores poisoning.

• Pediatric Infection and Vaccine
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• v.31 no.1
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• pp.136-139
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• 2024

Rhabdomyolysis is a syndrome that causes various complications due to the release of substances from muscle cells, often associated with preceding infectious diseases. We report the case of a 7-year-old Korean boy with recent severe acute respiratory syndrome coronavirus 2 infection, presenting with fever, chills, and generalized body aches, diagnosed as rhabdomyolysis. Additionally, we conducted a systematic review with the aim of delineating the disease spectrum, treatment, and outcomes. We identified seven reports that met the inclusion criteria. Among the cases, 5 had fever, with creatine kinase levels ranging from 3,717 and 274,664 IU/L. Two individuals received treatment in intensive care unit, 2 underwent renal replacement therapy, and 1 case has deceased. For children with coronavirus disease 2019 infection and muscle pain, a thorough examination of urine color and an assessment of muscle enzymes through blood tests can help diagnose and treat rhabdomyolysis, a condition that might otherwise be overlooked.

• Journal of Veterinary Clinics
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• v.32 no.1
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• pp.36-40
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• 2015

Equine colic is a major concern in equine industry due to high morbidity and mortality rates. The purpose of this study was to establish mortality rates during medically and surgically treated colic and to identify potential prognostic indicators for mortality of equine colic in Korea. The overall mortality rate was 18/119 (15%). The 93 cases (78%) were treated medically and 26 cases (22%) surgically out of the 119 records reviewed. The mortality rate in small intestinal lesion was 7/9 (78%) and strangulating lesion was 10/11 (91%). The significance between individual factors and prognosis of equine colic was not valid but high frequency rate was found in ${\leq}3$ years age (59%) and male (50%). In dead group showed higher, rectal temperature and heart rate than those of survived group. The mortality rate was significantly high in the pale mucous membrane color and severe pain and decreased intestinal motility. In laboratory factors, dead group showed lower level of platelet than survived group, and had higher values of RBC, hemoglobin, PCV with significance (P < 0.05). Group with increased enzyme activity of CK, LDH, glucose showed poor prognosis.