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http://dx.doi.org/10.5762/KAIS.2011.12.1.276

A Study on the Activation Conditions of Pancreatic Enzymes  

Kim, Dong-Chung (Department of Human Nutrition and Food Science, Chungwoon University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.12, no.1, 2011 , pp. 276-280 More about this Journal
Abstract
This study investigated the activation conditions of pancreatic enzymes from porcine pancreas. Duodenum induced the activation of pancreatic protease and lipase in pancreas. When 10% duodenum was added to pancreatic juice and the mixture was incubated at $30^{\circ}C$ for 90 min or at $25^{\circ}C$ for 4 hrs, the activities of pancreatic protese and lipase reached the peak. When the pancreatin was prepared by sequential process of enzymatic activation at $25^{\circ}C$ for 4 hrs, centrifugation, acetone precipitation and freeze-drying, the specific activities of pancreatic protease, lipase and amylase were 136, 116 and 400 U/mg-protein, respectively. The protease, lipase and amylase activities of the prepared pancreatin were 5.4, 58.0 and 16.0 times higher than those of USP standard, respectively.
Keywords
Pancreatin; Pancreatic enzymes; Activation; Duodenum;
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Times Cited By KSCI : 1  (Citation Analysis)
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