• Korean Journal of Microbiology
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• v.51 no.3
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• pp.263-270
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• 2015

A bacterial strain producing extracellular mannanases was isolated from soil of chestnut tree farm located in Gongju city of Korea by enrichment culture using Avicel as a carbon source. 16S rDNA sequence of the isolate YB-43 was highly homologous to those of genus Cellulosimicrobium strains with sequence similarities of above 99.6%. Mannanase productivity was significantly increased when the Cellulosimicrobium sp. YB-43 was grown in the presence of locust bean gum (LBG) or konjac. The mannanases were partially purified to be mannanase A (ManA) and mannanase C (ManC) by DEAE-Sepharose column and Q-Sepharose column chromatography from the culture filtrate of Cellulosimicrobium sp. YB-43 grown in LB medium supplemented with 0.7% LBG for 24 h. The partially purified ManA showed the highest activity at $55^{\circ}C$ and pH 6.5, while ManC activity was optimal at $65^{\circ}C$ and pH 7.5. ManA was stable up to $40^{\circ}C$ for 1 h, but ManC activity decreased significantly even after 1 h at $20^{\circ}C$. ManA and ManC showed difference from each other according to their substrate specificities and predominant products resulting from the mannanase hydrolysis for mannooligosaccharides. As a result, Cellulosimicrobium sp. YB-43 was found to produce two different kinds of mannanases.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.4
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• pp.195-203
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• 2006

An antagonistic strain, Burkholderia MP-1, showed antimicrobial activity against various filamentous plant pathogenic fungi, yeasts and food borne bacteria (Gram-positive and Gram-negative). The nucleotide sequence of the 16S rRNA gene (1491 pb) of strain MP-1 exhibited close similarity (99-100%) with other Burkholderia 16S rRNA genes. Isolation of the antibiotic substances from culture broth was fractionated by ethyl acetate (EtOAc) solvent and EtOAc-soluble acidic fraction. The antibiotic substances were purified through a silica gel, Sephadex LH-20, ODS column chromatography, and high performance liquid chromatography, respectively. Four active substances were identified as phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid and 4-hydroxyphenylacetate methyl ester by gas chromatographic-mass spectrum analysis. The minimum inhibition of concentration (MIC) of each active compound inhibited the growth of the microorganisms tested at 250 to $2500{\mu}g\;ml^{-1}$. The antimicrobial activity of crude acidic fraction at 1 mg of dry weight per 6 mm paper disc was more effective than authentic standard mixture (four active substances were mixed with the same ratio as acidic fraction) over a wide range of bacterial test.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.10
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• pp.4927-4935
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• 2012

Turmeric is a medicinal herb with various pharmacological activities. Curcumin, demethoxycurcumin(DMC) and bisdemethoxycurcumin(BDMC) were extracted from turmeric by dipping and ultrasonic wave method. And extraction efficiency was identified using solid phase extraction(SPE). The extracted sample were simultaneously separated and analyzed from three kinds of commercial $C_{18}$ reversed-phase high performance liquid chromatography using water and acetonitrile as mobile phase with isocratic elution mode. Flow rate 1.0mL/min, injection volume $10{\mu}L$ and column oven temperature $40^{\circ}C$ at 425nm wavelength has been conducted. From the experimental results, the optimum mobile phase composition of water/acetonitrile 50/50vol% using RS tech column. It is evident that the amount of curcuminoids extracted(extraction time 4h) by 100% MeOH was higher than any aqueous MeOH composition. Finally, in 100% water extraction, the amount(mAU${\times}$mim) of curcuminoids extracted by SPE was 14.3 and 24.5 times respectively higher than ultrasonic wave and dipping method. The shown results can be applied as sources for pharmaceuticals and functional material.

• The Journal of Engineering Geology
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• v.23 no.2
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• pp.95-104
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• 2013

This study proposes an efficient management plan for improving the water quality of agricultural reservoirs. Hongdong reservoir is located in an area that includes farmland, forest, residential housing, and livestock farms. The levels of pollutants in Hongdong reservoir were investigated with the aim of improving the water quality in the reservoir. The potential concentrations of total nitrogen (T-N) and total phosphorus (T-P) in Hongdong reservoir were 1.06-3.67 and 0.52-1.12 $g/m^2-d$, respectively. An analysis of leaching characteristics was performed under anaerobic conditions for 72 hours at $20^{\circ}C$. The concentrations of T-P and $PO_4$-P were measured by general leaching for a set period. In the case of T-P, the leaching was measured by irregular leaching due to denitrification of nitrite nitrogen ($NO_2$-N) and nitrate nitrogen ($NO_3$-N). A very small amount of $NH_3$-N on nitrogen ($N_2$) was measured due to the low pH in the column, as a consequence of the anaerobic conditions.

• Journal of Applied Biological Chemistry
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• v.58 no.1
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• pp.13-19
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• 2015

The roots of Achyranthes japonica Nakai were extracted with 100% aqueous and concentrated subfraction was separated with ultra-performance liquid chromatography-based activity profiling. Three compounds were isolated from the subfraction 5 through the repeated prep- high performance liquid chromatography column chromatography. According to the results of physico-chemical and spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as ecdysterone (1), 25S-inokosterone (2), and 25R-inokosterone (3). Three phytoecdysones were showed weak inhibitory activity for thymus and activation-regulated chemokine expression levels in tumor necrosis factor (TNF)-${\alpha}$ plus IFN-${\gamma}$ induced HaCaT cells, respectively. However, those compounds 1-3 were exhibited the most potent inhibition (80-95% at $200{\mu}g/mL$) against TNF-${\alpha}$ expression levels in A23187 plus phorbol-myrisrate acetate-induced RBL-2H3 cells. As result, 100% aqueous extract of A. japonica has an excellent anti-atopy activity. It could be used to a large range of functional anti-atopy cosmetics.

• Korean Journal of Food Science and Technology
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• v.47 no.1
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• pp.1-5
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• 2015

This study aimed to simultaneously determine various carotenoids from different colored paprika using an ultra performance liquid chromatograph (UPLC) equipped with a HSS T3 column. Analysis was performed at 450 nm using gradient conditions with acetonitrile/methanol/methylene chloride (65/25/10) and distilled water. We improved the peak resolution and performed carotenoid analysis within 30 min. We qualitatively analyzed 11 carotenoids (neoxanthin, capsorubin, violaxanthin, capsanthin, zeaxanthin, lutein, ${\alpha}$-cryptoxanthin, ${\beta}$-cryptoxanthin, lycopene, ${\alpha}$-carotene, and ${\beta}$-carotene). For the validation of UPLC methods, we validated the precision and accuracy of capsanthin. Capsanthin showed good linearity ($R^2$=0.9998) in the concentration range of $1-200{\mu}g/mL$ with 2.4 and $7.2{\mu}g/mL$ of limit of detection (LOD) and limit of quantification (LOQ), respectively. The relative standard deviation (RSD) for intra- and inter-day precision was less than 3.83%. Recovery was in the range of 91.86-99.87%. We quantitatively analyzed carotenoid contents from 8 different colored paprika (red, orange, yellow, and green). The most abundant carotenoids were capsanthin in red paprika, and zeaxanthin in orange, yellow, and green paprika.

• Journal of the Korean Geosynthetics Society
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• v.14 no.4
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• pp.139-146
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• 2015

This paper describes a effect of nano-bubble on removal of complex heavy metals, in order to remove pollutants of soil with copper (Cu) and lead (Pb) based on column test. It was conducted column test considering inflow and fine soil contents condition, and then it was analyzed removal effect of nano-bubble on Cu and Cu/Pb. The test result showed that the removal effect of the nano-bubble was higher than that of distilled water regardless of fine soil contents in case of Cu pollutant, and the removal effect of nano-bubble on heavy metals was found to have a significant impact on Pb than Cu in case of Cu/Pb pollutants. It was also analyzed that engineering properties (like a flow path and permeability) of soil due to void ratio has influenced a significant impact the pollutants removal of nano-bubble. The test results determined that nano-bubble can be applied as a flushing agent for priority target pollutant in complex heavy metals.

• Journal of the Korean Applied Science and Technology
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• v.8 no.1
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• pp.27-34
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• 1991

동백종실유(冬栢種實油)에서 컬럼크로마토그래피로 지질(脂質) 성분(成分)을 상호(相互) 분리(分離)하였다. 여기서 얻은 트리글리세리드의 일부(一部)를 취하여 알칼리로 가수분해(加水分解)하거나 또는 pancreatic lipase로 가수분해(加水分解) 하여, 트리글리세리드의 구성(構成) 지방산(脂肪酸) 또는 1, 3- 위치(位置)와 2-위치(位置)에 급합(給合) 한 지방산(脂肪酸) 조성(組成)을 조사(調査)하였다. 나머지 트리글리세리드는 16% $AgNO_3$ TLC로 이중결합선(二重結合敾)별로 나누었으며, 이렇게 나누어진 분획(分劃)을 다시 HPLC로 PN별로 재분획(再分劃) 하였다. 여기서 얻어진 획분(劃分)들 중 PN이 같은 것은 모두 모아서 알칼리 가수분해(加水分解)로 PN의 총지방산(總脂肪酸) 조성(組成)을, pancreatic lipase로 1,3 위치(位置)에 결합선(結合敾)한 지방산(脂肪酸)의 조성(組成)을 조사(調査) 하였다. 여기서 얻어진 결과(結果)로 부터 구성(構成) 트리그리셀리드 분자종(分子種)을 산출(算出)하였더니 다음과 같은 결과(算出)를 얻었다. 1) 종실유(種實油)는 투명(透明)한 액체(液體)로 그 함량(含量)이 73.5%였으며, 그 중 트리글리세리드가 94.8%, 극성(極性) 지질(極性)이 2.0%, 탄화(炭化) 수소(水素)가 1.8%였다. 2) $AgNO_3$-TLC로 트리글리세리드를 분획(分劃)하였더니 5개의 획분(劃分)을 얻었으며, 대부분(大部分)의 트리글리세리드가 이중(二重) 결합선(結合敾) 3${\sim}$5개인 band 2와 band 3에 80% 이상 존재(存在)하였다. 3) $AgNO_3-TLC$에서 얻은 각 획분(劃分)은 모두가 HPLC상에서 PN 46, 48 및 50으로 나누어졌으며 전체(全體) 분획(分劃)에서 PN 48이 78.13%로 제일 많았으며, 다음으로 PN 50이 12.04%였으며, PN 46이 9.83%였다. 4) 전체(全體) 트리글리세리드 분자종(分子種) 중에서 0.1mol% 이상을 차지하고 있는 분자종(分子種)이 43종(種)이었으며, OOO와 POO가 각각 39.8mol%와 25.8mol%로 제일 많았으며, 그 다음으로 OPO가 5.5 mol%, OOL가 4.8 mol%, POS가 3.9mol%, SOO가 3.5mol%. POL이 3.0mol%였다. 또 동백종실유(冬栢種實油)에 존재(存在)하는 트리글리세리드의 분자종(分子種)의 조성(組成)은 1,3-random, 2-random 분포설(分布說)에 따라 구성(分布說)되어 있었다.

• The Korean Journal of Pesticide Science
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• v.8 no.1
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• pp.46-53
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• 2004

Adsorption and leaching characteristics of hexaconazole were investigated to estimate the mobility potential of the compound in the soil environment. As well fitted to Freundlich adsorption isotherm, adsorptivity of hexaconazole, ranged 10.56-18.01 of Kf values, seemed high enough to be immobile in soil. This chemical leached more faster from fresh soil with rice plants. Most of $^{14}C$ (86-99% of originally applied $^{14}C$) was distributed within 5 cm soil depth from surface. Considering Koc values of 1,400-1,552 and Groundwater Ubiquity Score (GUS) indices of 1.25-1.35 as well as results from leaching experiment with soil column, hexaconazole falls into the category of improbable leacher, suggesting little mobility in soil.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.755-762
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• 2005

Biochemical amounts of RNA molecules can be synthesized in vitro, which is functionally equivalent or similar to those transcripts normally existing at extremely low levels in vivo. In this study we described a method for efficient preparation of pure T7 RNA polymerase from Escherichia coli strain BL21/pAR1219. The procedure, which used ammonium sulfate fractionation and preparative column chromatography on sephadex SP, was shown to be simple, rapid, and cost effective in comparison with other methods reported previously, Using the purified T7 RNA polymerase we were able to synthesize very long RNA transcript of 1.54 kb length, which is not feasible by conventional chemical synthesis. RNA molecule that was also synthesized by the purified T7 RNA polymerase, such as hammerhead ribozyme, retained its biochemical activity by cleaving the target RNA successfully in vitro. Thus, the procedure shown in this study can be useful to synthesize any length of RNA molecules in vitro in a simple and cost effective way for a variety of purposes.