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http://dx.doi.org/10.5762/KAIS.2012.13.10.4927

Solid Phase Extraction(SPE) of Curcuminoids from Turmeric by Optimization Analytical Condition  

Lee, Kwang Jin (KIOM, KM-Based Herbal Drug Research Group)
Ma, Jin Yeul (KIOM, KM-Based Herbal Drug Research Group)
Kim, Young Jun (Department of Cosmetic Science, Chungwoon University)
Kim, Young Sik (Department of Chemical Engineering, Kangwon National University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.13, no.10, 2012 , pp. 4927-4935 More about this Journal
Abstract
Turmeric is a medicinal herb with various pharmacological activities. Curcumin, demethoxycurcumin(DMC) and bisdemethoxycurcumin(BDMC) were extracted from turmeric by dipping and ultrasonic wave method. And extraction efficiency was identified using solid phase extraction(SPE). The extracted sample were simultaneously separated and analyzed from three kinds of commercial $C_{18}$ reversed-phase high performance liquid chromatography using water and acetonitrile as mobile phase with isocratic elution mode. Flow rate 1.0mL/min, injection volume $10{\mu}L$ and column oven temperature $40^{\circ}C$ at 425nm wavelength has been conducted. From the experimental results, the optimum mobile phase composition of water/acetonitrile 50/50vol% using RS tech column. It is evident that the amount of curcuminoids extracted(extraction time 4h) by 100% MeOH was higher than any aqueous MeOH composition. Finally, in 100% water extraction, the amount(mAU${\times}$mim) of curcuminoids extracted by SPE was 14.3 and 24.5 times respectively higher than ultrasonic wave and dipping method. The shown results can be applied as sources for pharmaceuticals and functional material.
Keywords
Turmeric; RP-HPLC; Separation; Purification; SPE;
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Times Cited By KSCI : 5  (Citation Analysis)
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