• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.109-113
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• 1998

In this study we tried to transform an antimicrobial peptide gene (Shiva) under the promoter of tomato phenylalanine ammonia-lyase (tPAL5) into tobacco and potato plants. Antimicrobial peptide gene was isolated originally from giant silk moth (Hyalophora cecropia) and modified ie nucleotide sequence to increase antimicrobial activity. Transgenic tobacco plants were regenerated and their seeds were tested on the media containing kanamycin (500 mg/L). The results of PCR amplification and genomic Southern blot hybridization confirmed the integration of construct (tPAL5 promoter-Shiva-NOS-GUS-NOS) into chromosome. We observed that one of the transgenic tobacco plants showed chromosome rearrangement when integrated. In case of potato transformation, the efficiency of regeneration was maximized at the medium containing Zeatin 2mg/L, NAA 0.01mg/L, GA$_3$ 0.1mg/L. We also observed the high expression of GUS (${\beta}$-glucuronidase) enzyme which was located next to the terminator sequence of nopaline synthase gene (NOS) in the vascular tissue of stem, leaves of transgenic potatoes. This result suggested that a short sequence of Shiva gene (120 bp) and NOS terminator sequence might be served as a leader sequence of transcript when translated.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.28 no.2
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• pp.37-44
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• 2010

Our country traditionally employed a defensive system against the aggression by foreign powers by using a town castle and mountain castle. The moat was one of such a defensive system; however, there is few research work on a moat in comparison with its long history. This study was carried out to see the process of the changes of the Wolseong Fortress at Gyuongju, focused on the moat of the scale and nature of the construction methods to analyze such a Wolseong Fortress at Gyuongju as a result of consideration through bibliographical study, on-site investigation, and interviews, etc. This research discovered some facts as follows: the moats of Wolseong fortress at Gyeongju are roughly divided into three types; the first one is a natural moat flowing curved by the south side of Wolseong using the natural stream[Namcheon]as it is; the second one is a pond-type moat made by digging up plane non-rectangular pond along stereobate of castle wall with lakefront built with stream pebbles, and the last one is masonry moat at the east side of Wolseong with chisel-trimmed granites orderly piled in a plane triangular form. Among these, the pond-type moat was identified at the east-north-west side of Wolseong and the pond slopes from east to west as a separate one constructed with the terraces.

• Journal of Korean Society of Forest Science
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• v.76 no.4
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• pp.357-360
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• 1987

1. The hair cells on adaxial surface turned out to be penetration way of Gleosporium Thea sinensis in tea plant. 2. The most parts of Gleosporium Thea sinensis generated in tea plant were young leaves with first-fifth leaves from tip of shoot. 3. The proper temperature for spore germination on leaf is $25-27^{\circ}C$, soaked by water for 12 hours. 4. For prevention of Gleosporium Thea sinensis the drug-spay in tea plant would be the most effective when sprayed at this temperature range, and the control of Gleosporium Thea sinensis would be possible through selection of tea plant with few hairs. 5. The treatment of bordeaux mixture to prevent growth of conidiospore was 48.3% more effective than in control plot which were not sprayed. 6. The effect of sprayed bordeaux mixture decreased to about 28.5% after one week of spray.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.221-228
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• 2021

The fall armyworm (FAW), Spodoptera frugiperda (Smith), is a notorious invasive migratory pest native to the tropics that has recently invaded South Korea with subsequent damage to cornfields. This study was conducted to evaluate the damage patterns on corn plants caused by the migratory and subsequent generations of FAW. The early migrant generation-related infestation rates reached an average of 13.2%, ranging from a minimum of 4.3% ('Allog-i') to a maximum of 33.0% ('Chodang'), depending on the corn cultivar. The proportion of FAW larvae-infested corn plants, in which the FAW survived until the pupal stage was 19.3%. The subsequent FAW generation caused considerable damage to the ears, resulting in 60% of ears with damaged kernels. This damage was markedly different from the nearly negligible damage caused by the migratory generation. The FAW larval dispersion was the most dynamic during the second instar stage and occurred along the same cornrow in line. In addition, we discuss the development of corn pant damage patterns caused by FAW. In summary, the results of the present study would provide useful basic information for the damage analysis of this pest for future studies.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.604-613
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• 2018

In recent years, numerous train derailment accidents caused by deterioration and high speed technology of railways have increased. Guardrails or barriers of railway bridges are installed to restrain and prevent the derailment of the train body level. On the other hand, it can result in a high casualties and secondary damage. Therefore, a Derailment Containment Provision (DCP) within the track at the wheel/bogie level was developed. DCP is designed for rapid installation because it reduces the impact load on the barrier and inertia force on the steep curve to minimize turnover, fall, and trespass on the other side track of the bridge. In this paper, DCP was analyzed using LS-Dyna with a parameter study as the impact loading location and interface contact condition. The contact conditions were analyzed using the Tiebreak contact simulating breakage of material properties and Perfect bond contact assuming fully attached. As a result, the Tiebreak contact behaved similarly with the actual behavior. In addition, the maximum displacement and flexural failure was generated on the interface and DCP center, respectively. The impact analysis was carried out in advance to confirm the DCP design due to the difficulties of performing the actual impact test, and it could change the DCP anchor design as the analysis results.

• Fire Science and Engineering
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• v.32 no.6
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• pp.40-45
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• 2018

The omnidirectional surveillance camera uses the object detection algorithm to level the object by unit so that broadband surveillance can be performed using a fisheye lens and then, it was a field experiment with a system composed of an omnidirectional surveillance camera and a tracking (PTZ) camera. The omnidirectional surveillance camera accurately detects the moving object, displays the squarely, and tracks it in close cooperation with the tracking camera. In the field test of flame detection and temperature of the sensing camera, when the flame is detected during the auto scan, the detection camera stops and the temperature is displayed by moving the corresponding spot part to the central part of the screen. It is also possible to measure the distance of the flame from the distance of 1.5 km, which exceeds the standard of calorific value of 1 km 2,340 kcal. In the performance test of detecting the flame along the distance, it is possible to be 1.5 km in width exceeding $56cm{\times}90cm$ at a distance of 1km, and so it is also adaptable to forest fire. The system is expected to be very useful for safety such as prevention of intrinsic or surrounding fire and intrusion monitoring if it is installed in a petroleum gas storage facility or a storing place for oil in the future.

• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.109-118
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• 2019

This study was carried out to investigate the vegetation of wetland and terrestrial lands in Jangdo wetland conserved area in Korea and to analyze the characteristics of the vegetation changes in the recent years. From the plant community, there were evergreen broad-leaved forests of the Machilus thunbergii, Castanopsis cuspidata, and Machilus thunbergii-Castanopsis cuspidata communities. Moreover, there were deciduous broad-leaved forests of the Salix koreensis, Mallotus japonicus, Mallotus japonicus-Pueraria thunbergiana and Celtis sinensis communities. Additionally, there were shrub forests of the Rosa multiflora-Rubus hirsutus, grassland of Molinia japonica-Miscanthus sacchariflorus and Miscanthus sacchariflorus-Imperata cylindrica communities, and plantation forest of the Pseudosasa japonica community. The area of the wetland vegetation (15%) was much narrower than that of the terrestrial land vegetation (85%). Comparing these results with those of the past 10 years, the wetland plant communities decreased by one-third and the proportion of neutral or dry plant communities increased. In order to mitigate landization succession of the wetland and maintain native wetland vegetation in this area, the expansion of the Salix koreensis community must be controlled to a suitable scale. In addition, it is urgently required to remove the invasive non-wetland plants, such as Pseudosasa japonica and Pueraria thunbergiana.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.724-732
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• 2020

Phytophthora capsici is the organism that causes Phytophthora blight which infects red pepper plants prolifically, ultimately leading to crop loss. A previous study revealed that Enterobacter asburiae ObRS-5 suppresses Phytophthora blight in both red pepper and Ligularia fischeri plants. In order to determine whether the induced systemic resistance (ISR) was triggered by pre-infection with the ObRS-5 strain, we conducted quantitative PCR using primers for PR1, PR4, and PR10, which correlate with systemic resistance in red-pepper plants. In our results, red pepper plants treated with the ObRS-5 strain demonstrated increased expression of all three systemic resistance genes when compared to controls in the glasshouse seedling assay. In addition, treatment of red peppers with the ObRS-5 strain led to reduced Phytophthora blight symptoms caused by P. capsici, whereas all control seedlings were severely affected. Perhaps most importantly, E. asburiae ObRS-5 was shown to induce the ISR response in red peppers without inhibiting growth. These results support that the defense mechanisms are triggered by ObRS-5 strain prior to infection by P. capsici and ObRS-5 strain-mediated ISR action are linked events for protection to Phytophthora blight.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.21 no.2
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• pp.15-26
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• 2021

Recently, as the introduction of cloud, mobile, and IoT has become active, there is a growing need for technology development that can supplement the limitations of traditional security solutions based on fixed perimeters such as firewalls and Network Access Control (NAC). In response to this, SDP (Software Defined Perimeter) has recently emerged as a new base technology. Unlike existing security technologies, SDP can sets security boundaries (install Gateway S/W) regardless of the location of the protected resources (servers, IoT gateways, etc.) and neutralize most of the network-based hacking attacks that are becoming increasingly sofiscated. In particular, SDP is regarded as a security technology suitable for the cloud and IoT fields. In this study, a new access control system was proposed by combining SDP and hash tree-based large-scale data high-speed signature technology. Through the process authentication function using large-scale data high-speed signature technology, it prevents the threat of unknown malware intruding into the endpoint in advance, and implements a kernel-level security technology that makes it impossible for user-level attacks during the backup and recovery of major data. As a result, endpoint security, which is a weak part of SDP, has been strengthened. The proposed system was developed as a prototype, and the performance test was completed through a test of an authorized testing agency (TTA V&V Test). The SDP-based access control solution is a technology with high potential that can be used in smart car security.

• Korean Journal of Ecology and Environment
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• v.55 no.3
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• pp.221-230
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• 2022

The spread of invasive alien plants is one of the most important causes of habitat and biodiversity loss. This research was conducted to secure basic data for the control of the invasive alien plant Solidago altissima. The following research objects were examined: the control; one-time mowing in June (1M6); two-time mowing in June and July (2M6M7); one-time mowing in Jun plus one-time Uprooting in July (2M6U7). The length growth in October, when the growth of S. altissima was completed, was in the order of control (199±19.96 cm), 1M6 (152±13.41 cm), 2M6U7 (54±3.17 cm), and 2M6M7 (45±19.96 cm). The 2M6M7 and 2M6U7 treatment was the most effective at inhibiting S. altissima of density, of 50.8% and 26.2% compared to the control, respectively. In contrast, one-time mowing (1M6) resulted in an increase in density compared to the control by 119.7%. In addition, this treatment also reduced flowering rate (%) of S. altissima. The most effective treatment among those tested was 2M6U7.