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http://dx.doi.org/10.11626/KJEB.2020.38.4.724

Induction of systemic resistance against Phytophthora blight by Enterobacter asburiae ObRS-5 with enhancing defense-related genes expression  

Kim, Dayeon (Bioremediation Team, National Institute of Agricultural Sciences)
Jeon, Yong Hee (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
Ahn, Jea-Hyung (Bioremediation Team, National Institute of Agricultural Sciences)
Ahn, Si Hyeon (Bioremediation Team, National Institute of Agricultural Sciences)
Yoon, Young Gun (Bioremediation Team, National Institute of Agricultural Sciences)
Park, In Cheol (Bioremediation Team, National Institute of Agricultural Sciences)
Park, Jin Woo (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
Publication Information
Korean Journal of Environmental Biology / v.38, no.4, 2020 , pp. 724-732 More about this Journal
Abstract
Phytophthora capsici is the organism that causes Phytophthora blight which infects red pepper plants prolifically, ultimately leading to crop loss. A previous study revealed that Enterobacter asburiae ObRS-5 suppresses Phytophthora blight in both red pepper and Ligularia fischeri plants. In order to determine whether the induced systemic resistance (ISR) was triggered by pre-infection with the ObRS-5 strain, we conducted quantitative PCR using primers for PR1, PR4, and PR10, which correlate with systemic resistance in red-pepper plants. In our results, red pepper plants treated with the ObRS-5 strain demonstrated increased expression of all three systemic resistance genes when compared to controls in the glasshouse seedling assay. In addition, treatment of red peppers with the ObRS-5 strain led to reduced Phytophthora blight symptoms caused by P. capsici, whereas all control seedlings were severely affected. Perhaps most importantly, E. asburiae ObRS-5 was shown to induce the ISR response in red peppers without inhibiting growth. These results support that the defense mechanisms are triggered by ObRS-5 strain prior to infection by P. capsici and ObRS-5 strain-mediated ISR action are linked events for protection to Phytophthora blight.
Keywords
biological control; Enterobacter asburiae ObRS-5; induced systemic resistance; red pepper plants; Phytophthora capsici;
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1 van Loon LC and EA van Strien.1999. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol. Mol. Plant Pathol. 55:85-97.   DOI
2 Macas-Rodrguez L, A Guzmn-Gmez, P Garca-Jurez and HA Contreras-Cornejo. 2018. Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system. FEMS Microbiol. Ecol. 94:fiy137.
3 Mahaffee WF and PA Backman. 1993. Effects of seed factors on spermosphere and rhizosphere colonization of cotton by Bacillus subtilis GB03. Phytopathology 83:1120-1125.   DOI
4 Kim DY, SY Lee, SH Ahn, JH Han and JW Park. 2020. Biological control of gom-chwi (Ligularia fischeri) Phytophthora root rot with Enterobacter asburiae ObRS-5 to suppress zoosporangia formation and zoospores germination. Plant Pathol. J. 36:244-254.   DOI
5 Livak KJ and TD Schmittgen. 2001. Analysis of relative gene expression data using realtime quantitative PCR and the 2∆∆C(T) Method. Methods 25:402-408.   DOI
6 Lopez-Gutierrez JC, S Henry, S Hallet, F Martin-Laurent, G Catroux and L Philippot. 2004. Quantification of a novel group of nitrate-reducing bacteria in the environment by real-time PCR. J. Microbiol. Methods 57:399-407.   DOI
7 Maleki M, L Mokhtarnejad and S Mostafaee. 2011. Screening of rhizobacteria for biological control of cucumber root and crown rot caused by Phytophthora drechsleri. Plant Pathol. J. 27:78-84.   DOI
8 Mansoori B and Z Banihashemi. 1982. Evaluating cucurbit seedling resistance to Phytophthora drechsleri. Plant Dis. 66:373.   DOI
9 Park SJ, GH Kim, AH Kim, H Lee, HW Gwon, J Kim, KH Lee and HT Kim. 2012. Controlling effect of agricultural organic materials on Phytophthora blight and anthracnose in red pepper. Res. Plant Dis. 18:1-9.   DOI
10 Parra G and JB Ristaino. 2001. Resistance to mefenoxam and metalaxyl among field isolates of Phytophthora capsici causing Phytophthora blight of bell pepper. Plant Dis. 85:1069-1075.   DOI
11 Yang R, X Fan, X Cai and F Hua. 2015. The inhibitory mechanisms by mixtures of two endophytic bacterial strains isolated from Ginkgo biloba against pepper Phytophthora blight. Biol. Control 85:59-67.   DOI
12 Elliott M, SF Shamoun, G Sumampong, D James, S Masri and A Varga. 2009. Evaluation of several commercial biocontrol products on European and North American populations of Phytophthora ramorum. Biocontrol Sci. Technol. 19:1007-1021.   DOI
13 Hardham AR. 2005. Pathogen profile Phytophthora cinnamomi. Mol. Plant Pathol. 19:260-285.   DOI
14 Park K, S Dutta, YS Park, MK Sang and SS Moon. 2016. Induction of systemic resistance and tolerance against biotic and abiotic stress in Chinese cabbage by cyclic peptides producing Bacillus vallismortis strain BS07M. pp. 200-205. In: Recent Trends in PGPR Research for Sustainable Crop Productivity. Scientific Publishers. New Delhi, India.
15 Soh JW, KS Han, SC Lee and JH Park. 2012. Inheritance of Resistance to Phytophthora capsici by Inoculums in Korean hot pepper. Res. Plant Dis.18:317-323.   DOI
16 Utkhede RS. 1986. Biology and control of apple crown rot caused by Phytophthora cactorum: A review. Phytoprotection 67:1-13.
17 van Loon LC and A van Kammen. 1970. Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. 'Samsum' and 'Samsun NN' II. Changes in protein constitution after infection with tobacco mosaic virus. Virol. 40:199-211.   DOI
18 Woo SM, HK Jung and SD Kim. 2006. Cloning and characterization of a cellulose gene from a plant growth promoting rhizobacterium, Bacillus subtilis AH18 against Phytophthora blight disease in red-peppr. Korean J. Microbiol. Biotechnol. 34:311-317.
19 Szczech M and M Shoda. 2006. The effect of mode of application of Bacillus subtilis RB14C on its efficacy as a biocontrol agent against Rhizoctonia solani. J. Phytopathol. 154:370-377.   DOI
20 Shirzad A, VF Mamaghani and M Pazhouhandandeh. 2012. Antagonistic potential of fluorescent Pseudomonads and control of crown and root rot of cucumber caused by Phythophtora drechsleri. Plant Pathol. J. 28:1-9.   DOI
21 Joo GJ. 2005. Production of an anti-fungal substance for biological control of Phytophthora capsici causing Phytophthora blight in red-peppers by Streptomyces halstedii. Biotechnol. Let. 27:201-205.   DOI
22 Chernin L, Z Ismailov, S Haran and I Chet. 1995. Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens. Appl. Environ. Microbiol. 61:1720-1726.   DOI
23 Haggag W and S Timmusk. 2008. Colonization of peanut roots by biofilm-forming Paenibacillus polymyxa initiates biocontrol against crown rot disease. J. Appl. Microbiol. 104:961-969.   DOI
24 Heil M and RM Bostock. 2002. Induced systemic resistance(ISR) against pathogens in the context of induced plant defences. Ann. Bot. 89:503-512.   DOI
25 Hong CE, SY Kwon and JM Park. 2016. Biocontrol activity of Paenibacillus polymyxa AC-1 against Pseudomonas syringae and its interaction with Arabidopsis thaliana. Microbiol. Res. 185:13-21.   DOI
26 Jetiyanon K and P Plianbangchang. 2013. Lipopolysaccharide of Enterobacter asburiae strain RS83: A bacterial determinant for induction of early defensive enzymes in Lactuca sativa against soft rot disease. Biol. Control 67:301-307.   DOI
27 Kim BS. 2014. Phytophthora blight of pepper and genetic control of the disease. Cur. Res. Agric. Life Sci. 32:111-117.   DOI
28 Kim HY and BH Min. 2003. Effects of arbuscular mycorrhizal fungus, Glomus manihot, on plant growth and nutrient uptake of pepper seedlings. Korean J. Environ. Biol. 21:292-296.
29 Bunemann EK, G Bongiomo, Z Bai, RE Creamer, GD Deyn, R de Goede, L Fleskens, B Geissen, TW Kuyper, P Mader, M Pulleman, W Sukkel, JW van Groenigen and L Brussaard. 2018. Soil quality - a critical review. Soil Biol. Biochem. 120:105-125.   DOI
30 Kim JS, WI Kim, HJ Jee, JG Gwang, CK Kim and CK Shim. 2010. Evaluation of resistance in hot pepper germplasm to Phytophthora blight on biological assay. Hortic. Sci. Technol. 28:802-809.
31 Cooley MB, WG Miller and RE Mandrell. 2003. Colonization of Arabidopsis thaliana with Salmonella enterica and enterohemorrhagic Escherichia coli O157:H7 and competition by Enterobacter asburiae. Appl. Environ. Microbiol. 69:4915-4926.   DOI
32 Bae SJ, TK Mohanta, JY Chung, M Ryu, G Park, S Shim, SB Hong, H Seo, DW Bae, I Bae, JJ Kim and H Bae. 2016. Trichoderma metabolites as biological control agents against Phytophthora pathogens. Biol. Control 92:128-138.   DOI
33 Baum C, W El-Tohamy and N Gruda. 2015. Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi: a review. Sci. Hortic. 187:131-141.   DOI
34 Berner I, SK Rapp, G Jung and G Winkelmann. 1988. Characterization of ferrioxamine E as the principal siderophore of Erwinia herbicola (Enterobacter agglomerans). Biol. Met. 1:51-56.   DOI
35 Jee HJ, WD Cho and CH Kim. 2000. Phytophthora diseases in Korea. Rural Development Administration, National Institute of Agricultural Science and Technology, Plant Pathology Division. Suwon, Korea. pp. 87-114.
36 Kang DS, KJ Min, AM Kwak, SY Lee and HW Kang. 2017. Defense response and suppression of Phytophthora blight disease of pepper by water extract from spent mushroom substrate of Lentinula edodes. Plant Pathol. J. 33:264-275.   DOI
37 Zhang YL, DW Li, ZH Gong, JE Wang, YX Yin and JJ Ji. 2013. Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae). Genet. Mol. Res. 12:3605-3621.   DOI
38 Porter SS, R Bantay, CA Friel, A Garoutte, K Gdanetz, K Ibarreta, BM Moore, P Shetty, E Siler and ML Friesen. 2019. Beneficial microbes ameliorate abiotic and biotic sources of stress on plants. Funct. Ecol. 34:2075-2086.
39 Kim ST and SC Yun. 2011. Biocontrol activity of Myxococcus sp. KYC 1126 against Phytophthora blight on hot pepper. Res. Plant Dis. 17:121-128.   DOI
40 Lee SJ, YJ Park, HT Kim and BS Kim. 2010. The race differentiation of Phytophthora capsici in Korea. Res. Plant Dis. 16:153-157.   DOI
41 Lim JH, HY Jung and SD Kim. 2009. Development of the microbial consortium for the environmental friendly agriculture by the antagonistic rhizobacteria. J. Appl. Biol. Chem. 52:116-120.   DOI
42 Kloepper JW, CM Ryu and S Zhang. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology 94:1259-1266.   DOI
43 Lee SH, YE Cho, SH Park, K Balaraju, JW Park, SW Lee and K Park. 2013. An antibiotic fusaricidin: a cyclic depsipeptide from Paenibacillus polymyxa E681 induces systemic resistance against Phytophthora blight of red-pepper. Phytoparasitica 41:49-58.   DOI
44 Kang HJ, KH Jeong, K Ahn, CU Han, SH Kim and Y Kim. 2011. Damage analysis and establishment of control threshold for Phytophthora blight of hot pepper(Capsicum annuum). Res. Plant Dis. 17:1-12.   DOI
45 Park JW, S Jahaggirdar, YE Cho, KS Park, SH Lee and KS Park. 2010. Evaluation of Bacillus subtilis native strains for plant growth promotion and induced systemic resistance in tomato and red-pepper. Korean J. Pestic. Sci. 14:407-414.