Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Induction of systemic resistance against Phytophthora blight by Enterobacter asburiae ObRS-5 with enhancing defense-related genes expression

역병에 대한 Enterobacter asburiae ObRS-5 처리의 유도저항성 발현

  • Kim, Dayeon (Bioremediation Team, National Institute of Agricultural Sciences) ;
  • Jeon, Yong Hee (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Ahn, Jea-Hyung (Bioremediation Team, National Institute of Agricultural Sciences) ;
  • Ahn, Si Hyeon (Bioremediation Team, National Institute of Agricultural Sciences) ;
  • Yoon, Young Gun (Bioremediation Team, National Institute of Agricultural Sciences) ;
  • Park, In Cheol (Bioremediation Team, National Institute of Agricultural Sciences) ;
  • Park, Jin Woo (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
  • 김다연 (국립농업과학원 환경개선미생물연구단) ;
  • 전용희 (국립농업과학원 농업생물부 농업미생물과) ;
  • 안재형 (국립농업과학원 환경개선미생물연구단) ;
  • 안시현 (국립농업과학원 환경개선미생물연구단) ;
  • 윤영건 (국립농업과학원 환경개선미생물연구단) ;
  • 박인철 (국립농업과학원 환경개선미생물연구단) ;
  • 박진우 (국립농업과학원 농업생물부 농업미생물과)
  • Received : 2020.12.05
  • Accepted : 2020.12.24
  • Published : 2020.12.31
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Abstract

Phytophthora capsici is the organism that causes Phytophthora blight which infects red pepper plants prolifically, ultimately leading to crop loss. A previous study revealed that Enterobacter asburiae ObRS-5 suppresses Phytophthora blight in both red pepper and Ligularia fischeri plants. In order to determine whether the induced systemic resistance (ISR) was triggered by pre-infection with the ObRS-5 strain, we conducted quantitative PCR using primers for PR1, PR4, and PR10, which correlate with systemic resistance in red-pepper plants. In our results, red pepper plants treated with the ObRS-5 strain demonstrated increased expression of all three systemic resistance genes when compared to controls in the glasshouse seedling assay. In addition, treatment of red peppers with the ObRS-5 strain led to reduced Phytophthora blight symptoms caused by P. capsici, whereas all control seedlings were severely affected. Perhaps most importantly, E. asburiae ObRS-5 was shown to induce the ISR response in red peppers without inhibiting growth. These results support that the defense mechanisms are triggered by ObRS-5 strain prior to infection by P. capsici and ObRS-5 strain-mediated ISR action are linked events for protection to Phytophthora blight.

본 연구에서는 기 선발한 Enterobacter asburiae ObRS-5 균주를 1×108 cfu mL-1 농도로 고추에 관주 처리했을 때 Phytophthora capsici에 의한 고추역병을 74.6% 방제하는 효과가 있었다. E. asburiae ObRS-5 균주에 의한 고추역병 방제 메커니즘을 확인하기 위해 고추의 PR1, PR4 및 PR10 유전자를 특이적으로 증폭하는 프라이머를 이용하여 quantitative PCR을 수행하였다. 그 결과 E. asburiae ObRS-5 균주를 처리한 고추에서 대조구와 비교하여 상기 세 가지 유전자의 발현이 모두 높은 수준을 유지하였다. 또한 E. asburiae ObRS-5 균주는 고추의 생육을 억제하지 않으면서 ISR 반응을 유도하는 것으로 나타났다. 이러한 결과를 통하여 P. capsici이 침입할 때 E. asburiae ObRS-5 균주가 매개하는 ISR 메커니즘을 통해 Phytophthora 역병의 제어가 가능한 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 연구사업(과제번호: PJ01259502) 지원에 의해 수행되었습니다.

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