• Korean Journal of Ecology and Environment
• /
• v.45 no.3
• /
• pp.314-321
• /
• 2012

To quantitatively assess the effects of turbidity on egg development and larval fish survival, a laboratory fish rearing experiment was applied to different life stages (newly hatched larval stage, juvenile stage and pre-adult stage) of the Zacco platypus, one of the most universal and tolerant species in Korea. According to the stress index of turbidity in water with exposure time, three different treatments, including a reference condition (1~7 NTU) as well as intermediate (20~150 NTU) and high turbidity conditions (400~1,000 NTU) were applied, and egg hatching and larval fish mortality rates were observed. The mortality rates of newly hatched larval fish were significantly different among treatments (ANOVA, $F_{2,3}$=17.79, p<0.05). Average rates of survival to hatching were 20.9% (${\pm}0.1%$) for reference condition, 11% (${\pm}6.9%$) for intermediate level and 3.2% (${\pm}3.7%$) for high level conditions, respectively. A sudden change of mortality at the high level was observed within 5 days of the experiment. About 84% of juvenile fish survived until 20 experimental periods under conditions of reference turbidity, while survival under conditions of intermediate turbidity was over 80% of larval fish until day 13 of the experiment, but dropped to less than 10% after day 14 and 15 of two experiments. Fish mortality appeared from day 6 of the high turbidity experiment, and 50% mortality was achieved at day 9 to 10 of experiment. Full mortality occurred at day 14 of the experiment (RM-ANOVA, $F_{2,38}$, p<0.005). In the pre-adult stage experiment, no mortality was observed during the experiment at reference level treatment (20 days), while only slight mortality rates were observed for both intermediate and high levels until day 5 of the experiment, however, no further fish died in either experiment. It was significantly different compared to reference condition (RM-ANOVA, $F_{2,20}$=8.28, p<0.01), but no difference was observed between intermediate and high level conditions. Consequently, this tolerant species has been determined to be well adapted to high levels of turbidity in its adult stage, but more vulnerable throughout earlier life stages.

• Journal of Applied Biological Chemistry
• /
• v.60 no.2
• /
• pp.155-159
• /
• 2017

This study was to evaluate the effect of the moisturizing cream containing five kinds of plants extracts for human facial improvements such as hydrating ability, transepidermal water loss and erythema reaction. The antioxidant activities of yellowish natural colorants, extracted from five kinds of plants (Ceramium kondoi, Phellodendron amurense, Carthamus tinctorius, Gardenia jasminoides for. grandiflora and Aphis chinensis) were tested. Twenty-one volunteers were subjected to analyze the effects, and each result was determined by treating the complex cream containing plants extracts on their skin in three times. The moisture content on skin was significantly increased more than 50% by treating the cream containing five kinds of plants extracts. The transepidermal water loss was reduced and the level of the skin erythema reaction was improved after treatment of the complex cream. Survey analysis showed that the volunteers had highly satisfaction on the tested creams. The results suggest that the complex cream containing five plants extracts had an improved effect on facial skin, and therefore can be used as moisturizing ingredients in the related industry.

• Korean Journal of Ichthyology
• /
• v.11 no.2
• /
• pp.155-162
• /
• 1999

To study the distribution of larva and juvenile of the sand eel, Ammodytes personatus in the Kangwon coastal, samples were collected by the larva net (RN 80 net) and small drag net at 60 stations from January 1996 to December. Appearance season of larva and juvenile of sand eel was appeared untill the January to June in the Kangwon-do coast, and the densities of them were high in June. Appearance phase, length frequency distribution of larva and juvenile of sand eel were distributed that the average standard length 10.0~30.0mm in the temperature range of $5.0{\sim}6.0^{\circ}C$ at the January to March, and April to June occurred the average standard length 40.0~80.0 mm in the temperature range of $7.2{\sim}12.0^{\circ}C$. It was distributed in southern part of Kangwon-do coast until the February to March 1996. And association of the sand eel migrated to the northern and middle part of Kangwon-do coast at April when surface water temperature was higher than $7.2^{\circ}C$. It was considered that the migration of the abundance larva and juvenile of sand eel in this area is mainly affected by the water temperature.

• Korean journal of applied entomology
• /
• v.34 no.4
• /
• pp.300-307
• /
• 1995

This study was carried out to investigate the life cycle of Cephonodes hylas (Linne), that is damaging to Gardenia jasminoides Ellis in the seedbed at Namhae during 1984 and 1985. Results are summarized as follows. The eggs were laid in mass on the leaf surface of the host plants. The oviposition period lasts for 26 days after late-May. The average number of eggs and average hatching rate were 20.36 and 97.61% , respectively. It takes 14-16 days until hatching. The egg shape was oval which its size was 1.01 mm in diameter and 1.48 mm in length. The body length of each larval instar from the 1st to the 5th were $3.52\pm$1.01, $6.46\pm$0.45, $13.19\pm$4.27, $24.08\pm$2.22 and $43.71\pm$5.62 mm, respectively. The average weight of each larval instar were $13.34\pm$2.41, $29.83\pm$6.88, $76.64\pm$31.31, $292.09\pm$107.70 and 2,$144.84\pm$760.70 mm, respectively. The larval period was observed from July to October, and total duration of larval stage was 116 days in which duration of each larval instar from the 1st to the 5th were 12, 36, 32, 16 and 20 days, respectively. During larval stage, a larvae feed on 17~18 seedlings. The 5th instar larvae built its place in soil for overwinter and became pupa. The body length of pupa was 33.6 mm and average weight was 2,032 mg Adult was emerged from overwintened pupa from mid through late-May in the followings year. The sex ratio of female was 42.82% and the color ratio of brown was 89.93%.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.9
• /
• pp.1131-1138
• /
• 2009

In this study, the breads with medicinal herbs (MH) composites showing cognitive function were prepared and their characteristics were examined. Fifteen kinds of medicinal herbs were extracted with hot water and divided into 3 groups (MH-1, MH-2, MH-3) based on their contents. All groups showed cognitive function in terms of promoting muscarinic receptor, NMDA receptor and acetylcholinesterase inhibition. In the preparation of breads containing MH extracts of various contents (0, 10, 20, 30, and 50%), there were no significant difference among loaf weight of all groups, but loaf volume of all groups were significantly (p<0.05) decreased with increasing content of MH extracts compared to the control group. The "a" and "b" values of bread crumb increased with the content of MH extracts while "L" value decreased, but these values of bread crust were similar to the control group. The most improvements in hardness, fracturability, cohesiveness, gumminess and chewiness of bread were noticed by the addition of 10$\sim$20% MH extracts, while adhesiveness and springiness of bread were the most by the 30% addition ones. Through the sensory evaluation, it was revealed that appearance, mouth feeling, taste, overall preference and crumb texture of bread were not significantly different (p<0.05) between control and those with the 10% addition ones. Therefore the 10% addition ones, which got similar scores to control, will make the most desirable product.

• Proceedings of the KIEE Conference
• /
• 2015.07a
• /
• pp.1098-1099
• /
• 2015

${\bullet}$ 시험대상: 영월태양광 4,990kW(998kW ${\times}$ 5호) ${\bullet}$ 13.07.31 연계기술검토의뢰 ${\bullet}$ 13.08.28 한전검토결과: 인근변전소 영월S/S(40MW) 초과로 10.8km 지점의 평창 D/L 평창간 347호 주진S/S에 다음의 보완대책과 연계 권고 ${\cdot}$ 문제점: 적정전압이탈 및 순시전압변동률 유지불가 ${\cdot}$ 연계기준만족을 위해 한전에서 제시한 보완대책 : 계통용량증설(설비보강), 연계용량 감소 및 전용선로 연계 ${\bullet}$ 14.01.09 분산형설치자의 용역사(에이스기술단)에서 송배전용전기설비 이용규정과 연계기준 만족을 위한 보완대책 제안 ${\cdot}$ 태양광발전시스템 인버터 자동전압(역률/무효전력) 조정기능 및 감시설비 구비 ${\cdot}$ 최적 공통연결점 선정: 분산형전원으로 부터 전기적으로 가장 가까운 영월D/L 영월S/S측에 개폐장치설치 하고 주진S/S 측으로 연계 ${\bullet}$ 14.01.29~06.19 한전(전력연구원)의 유효성 검토 ${\cdot}$ 보완대책 기술적 유효(실증시험 시행 후 시행공문 발송 예정) ${\bullet}$ 14.08.08 송배전용전기설비 이용규정 개정에 따른 협의 ${\cdot}$ 변압기 대당 20MW에서 25MW ${\cdot}$ 변전소 전체 60MW에서 75MW까지 허용 ${\cdot}$ 영월 S/S 측 문곡/영월 D/L 연계 공사 협의 ${\bullet}$ 14.10.04 사용전검사, 한전계통연계운전 시작 ${\bullet}$ 인버터 고정역률(99.9~100%) 운전 시험 ${\cdot}$ 14.11.21 22.9kV 계전기(SEL-751) 5대, 15분 간격 자료저장 ${\cdot}$ SEL-751/USB to RS232C/노트북 15분간격 측정(첨부) ${\bullet}$ 14.03.07 인버터 역률 95.0% 조정 15분간격 측정(첨부) ${\bullet}$ 변압기탭 선정과 인버터전압조정협조시험 계획 ${\cdot}$ 변압기탭을 현장에서 상하 1탭씩 변경 ${\cdot}$ 인버터역률을 인버터공급사에서 원격조정 ${\cdot}$ 22.9kV 보호계전기 SEL-751, 15분 간격 측정 ${\cdot}$ 인버터 370V 계전기 K-PAM DG3000/현장감시설비 연계 ${\cdot}$ 15분 간격 측정 저장 ${\cdot}$ 현장감시설비/인터넷 연결 원격측정(첨부예정)

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.3 no.2
• /
• pp.137-147
• /
• 1970

From November 1968 to March 1970, a series of drift bottle experiments were carried out in waters adjacent to and in Chinju Bay with the following results. Of the bottles released, $50\~69$ per cent were recovered. 1. The circulation of Chinju Bay is usually caused by the tidal current except during the winter season when the northwest monsoon prevails to cause a wind-drift current. 2. Sea water in the southern part of Chinju Bay flows northward at ebb tide. The ebb current east of the central submarine bank in Chinju Bay flows northeastward toward Samchonpo Channel through the eastern depression of the bank contributing to form a cyclonic eddy. The ebb current west of the bank, however, flows northward toward Noryang Channel through the western depression of the bank. 3. The ebb current nea. the southernmost part of Chinju Bay flows eastward toward Chijok Channel. 4. At flood tide, the main stream of the tidal current in Noryang Channel flows eastward. Turning smoothly to the right, the southern branch of the flood current flows southward through the depression and along the isobaths at the western margin of the central submarine bank, while the northern branch, turning to the left, flows into the Chin-gyo Bay of Hadong. 5. flood current in the eastern area of Kwang-yang Inlet runs northeastward toward Noryang Channel. A small eddy develops near Kwanumpo of Namhae Island. 6. The results suggest that such a drift bottle experiment can be recommended for the attestation of currents, although it is not suitable for a quantitative study of coastal currents.

• Herbal Formula Science
• /
• v.20 no.2
• /
• pp.177-186
• /
• 2012

In this study, we selected some herbal formulation about a series of Chijasi-tang in Dongeuibogam by using web-based open program;Prescription Lineage Graph (http://164.125.206.43/PrescriptionLineageGraph.aspx). And we compared and analyzed the changes of efficacy, major target symptoms of each herbal formulation according to compositional variation of each herbal formulation. Chijasi-tang, first appeared in Sanghanlun, consists of Capejasmine and Fermented soybean, and it is mainly used to treat insomnia due to vexation, heartburn and yellow greasy tongue fur. Capejasmine can clear away irritable feverish sensation in chest by flowing downward the heat, and Fermented soybean can disperse stagnated heat throughout the chest by expelling stagnated heat from the exterior In the case of the heat stagnation caused by relapsing of disease due to overstain, Chisijisil-tang can be used. And if symptom appear more on the upper or exterior than a case of Chisijisil-tang, Seosisi-tang could be more suitable, if half exterior and half interior, Omae-tang could be for it. In addition, if symptom caused by relapsing due to improper diet, Chisijihwang-tang could be proper formulation. In the case of the heat stagnation body inside and jaundice, if it is caused by alcohol, Chijadaehwang-tang could be used for the purpose of urgent purgation, Galchul-tang would be suitable for helping the function of the spleen and the stomach and for treatment of damp-heat of the spleen and the stomach. And if it is caused by pandemic infection, Jangdal-hwan would be good formulation for it. Samhwangseokgo-tang and Yangdokchija-tang could be appropriate formulation for the raging of noxious heat and pathogenic fire caused by febrile disease with toxic yang. Daehwangeum-ja is for severe constipation due to heat-dryness with stagnated fever, Haebaek-tang is appropriate for severe diarrhea due to heat type change of Soeum. According to the result of our investigation, although there are various target causes and symptoms of each herbal formulations, whatever pathogenetic cause is, the stagnated heat in interior side is the basis of symptoms. Therefore, the purpose of including Chijasi-tang in composition of each herbal formulation is treatment of the stagnated heat. For such reason, on the fundamental or ancillary basis of Chijasi-tang plus some herbs for each therapeutic purpose.

• Journal of Acupuncture Research
• /
• v.25 no.6
• /
• pp.95-107
• /
• 2008

Objectives : The main purpose of this research is to observe the effects of herbal acupuncture(HA; herbal acupuncture) with Chijadaehwangtang applied to the Gansu($BL_{18}$) on D-galactosamine-induced liver injury in rats. Methods : According to HA concentration, the experimental rats were divided 5 groups(control group, saline group, CP-1, CP-2, CP-3 group). In the control group, we first injected D-galactosamine and then didn't treated. In the saline group, we first injected D-galactosamine and then injected saline. In the CP-1, CP-2, CP-3 group, we first injected D-galactosamine and then injected HA with Chijadaehwangtang applied to the Gansu($BL_{18}$), each 25.3mg/kg, 12.7mg/kg, 5.1mg/kg. HA with Chijadaehwangtang was treated at $20{\mu}{\ell}$ every second day, total 10 times in 20 days. We observed the changes of $\gamma$-GTP, GOT, GPT, total bilirubin, LDH, ALP, total cholesterol, triglyceride, SOD, Catalase and hepatic tissues. Results : 1. In the changes of $\gamma$-GTP, GOT contents, as compared with control group, CP-2 group was significantly decreased. 2. In the changes of GPT content, CP-1, CP-2, CP-3 groups as compared with control group were significantly decreased. 3. In the changes of LDH content, CP-3 group as compared with control group was significantly decreased. 4. In the changes of ALP content, all groups as compared with control group were significantly decreased. 5. In the changes of SOD, Catalase contents, CP-2 group as compared with control group was significantly increased. 6. In the morphological and histopathological changes of hepatic tissues, CP-2, CP-3 groups as compared with control group were improved. Conclusions : we observed HA with Chijadaehwangtang applied to the Gansu($BL_{18}$) can be effective treatment on hematological recovery and regenerative process in morphological liver change. Further studies about their underlying mechanism and concentrations may be needed to use HA with Chijadaehwangtang for liver injury clinically.

• The Journal of Internal Korean Medicine
• /
• v.31 no.2
• /
• pp.189-200
• /
• 2010

Objectives : Regulatory T cells can reduce inflammation and allergic reactions through their inhibitory functions. Gardeniae Fructus(GF) is a Heat-clearing herb used in traditional Korean medicine, and a wide range of studies on its antiinflammatory effects are being carried out. The authors investigated the effect that Gardeniae Fructus has on regulatory T cells. Methods : The authors screened 14 herbs for their effects on regulatory T cells. 100mg of each herb were separately dissolved in 1ml of sterile saline and the supernatant was harvested after 10 minutes of centrifuge at 15,000 rpm. The supernatant was filtered through a 0.2 ${\mu}m$ syringe filter, and the resulting stock was refrigerated at $4^{\circ}C$. The stock was diluted before testing and used at a final concentration of $0.01{\mu}g/ml$. CD4+CD25+ T cells from healthy BALB/c spleens were used as natural regulatory T cells (nTreg), and CD4+CD25- T cells were used as reactive T cells. CD4+CD25+ and CD4+CD25- T cells were activated with anti-CD3e ($10{\mu}g/m{\ell}$)/anti-CD28 ($1{\mu}g/m{\ell}$) and cultured. IL-10 from supernatant of the culture medium was measured by IL-10 cytokine ELISA. The percentages, cell numbers, phenotype and function of CD4+CD25+ Treg cells were determined by flow cytometry. Results : Gardeniae Fructus was shown to be the most potent herb among the 14 herbs tested for suppressing CD4+CD25- reactive T cell proliferation by stimulating CD4+CD25+ natural regulatory T cells. Gardeniae Fructus induces IL-10 secretion increase by stimulating CD4+CD25+ natural regulatory T cells, and indirectly suppresses CD4+CD25- reactive T cell proliferation through increasing CD25 (IL-2 receptor $\alpha$) expression and thus promoting bonding with IL-2. Gardeniae Fructus did not directly affect CD4+CD25- reactive T cell proliferation. Conclusions : Gardeniae Fructus suppressed reactive T cell proliferation through inducing increases in IL-10 secretion and CD25 (IL-2 receptor $\alpha$) expression.