• The Journal of Korea Assosiation for Disability and Oral Health
• /
• v.14 no.2
• /
• pp.92-96
• /
• 2018

Drug-induced gingival overgrowth is an abnormal increase of gingival tissues caused as a side effect of systemic medication. This report presents a severe case of valproate-induced gingival overgrowth combined with ulcerative and hemorrhagic lesions in a patient with Lennox-Gastaut syndrome. Considering the patient's limited cooperative ability, gingivectomy and excisional biopsy under general anesthesia were performed. The lesions were successfully treated without recurrence. When gingival enlargement does not subside with nonsurgical treatments, surgical procedure and excisional biopsy are to be performed. Postoperative management of oral hygiene is critical to prevent recurrence.

• The Journal of Korean Academy of Prosthodontics
• /
• v.55 no.4
• /
• pp.361-371
• /
• 2017

Purpose: We aimed to investigate the gene expression of human gingival fibroblasts on microgroove surface using DNA microarray. Materials and methods: Microgrooves were applied on grade II titanium discs to have 0/$0{\mu}m$ (NE0, control group), 60/$10{\mu}m$ (E60/10, experimental group) of respective width/depth by photolithography. The entire surface of the microgrooved Ti substrata was further acid etched and used as the two experimental groups in this study. Human gingival fibroblasts were cultured in the experimental group and the control group, and total RNA was extracted. The oligonucleotide microarray was performed to confirm the changes of various gene expression levels between experimental group and control group. Changes of gene expression level were determined at the pathway level by mapping the expression results of DNA chips, using the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. Results: Gene expression levels on E60/10 and NE0 were analyzed, there were 123 genes showing significant differences in expression more than 1.5 times on E60/10 microgrooved surface compared to NE0 surface, and 19 genes showing significant differences in expression more than 2 times. The KEGG pathway analysis confirmed the changes in gene expression levels under experimental conditions. Cell signaling, proliferation, and activity among the various gene expression results were identified. Conclusion: Microgrooved surfaces induce gene expression changes and related cell signaling. According to the results of this study, microgrooves can be used as the surface of various biomaterials which need to improve cell activity through gene expression changes and activation of cell signaling.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.43 no.4
• /
• pp.452-460
• /
• 2016

Burkitt lymphoma (BL) is an aggressive form of non-Hodgkin's B-cell lymphoma found primarily in the pediatric population. In the oral cavity, this tumor can grow rapidly and often brings about facial swelling or development of an exophytic mass involving the jaws. A 5-year-old boy was referred for swelling and pain in the left mandibular area. The patient showed diffuse swelling on the left side of the mandible and firm-moderate tenderness upon palpation. An intraoral examination showed moderate mobility and sensitivity to percussion on the left primary first and second molars, without severe caries. A radiographic examination revealed complete loss of the lamina dura on the left primary second molar and permanent first molar. There was a radiolucent osteolytic lesion and destruction of the cortical bone of the left mandibular body. Based on the clinical, radiographic, and immunohistochemical findings, the patient was diagnosed with BL, and was referred to a pediatrician for systemic evaluation and intensive chemotherapy. Even before the completion of chemotherapy, the swelling resolved and the displaced teeth were relocated to a normal position. This patient showed a good prognosis due to prompt diagnosis and intensive chemotherapy. Early diagnosis and referral for treatment can prevent the development of BL.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.38 no.2
• /
• pp.90-96
• /
• 2022

Purpose: The purpose of this study was to investigate antimicrobial activity of extracts from shiitake mushroom against periodontopathogens and its cytotoxicity for human gingival fibroblast. Materials and Methods: Shiitake mushroom was soaked in water and acetone, and the supernatant was dried to collect its extract. The susceptibility of periodontopathogens for the extracts was investigated. Human gingival fibroblast was treated with the extracts, and the cell viability was measured CCK-8 solution. Results: The water extract from shiitake mushroom significantly reduced the growth of periodontopathogens at 2.5 mg/ml (P < 0.05). The acetone extract significantly inhibited the growth of Porphyromonas gingivalis and Tannerella forsythia at 0.32 mg/ml and Treponema denticola growth at 0.64 mg/ml (P < 0.05). The cytotoxicity of the extract was shown at a concentration of 2.5 mg/ml. The extracts with a concentration of 1.25 mg/ml appeared to be reduce cell viability after 4 h. Conclusion: The extracts of shiitake mushroom have antimicrobial activity against periodontitis-causing bacteria and relieving inflammation. Therefore, the extracts may be a candidate for preventing and treating periodontal disease.

• The Journal of Korean Academy of Prosthodontics
• /
• v.62 no.3
• /
• pp.224-233
• /
• 2024

The patient was a 69-year-old male who had decreased VDO and overbite of the anterior teeth due to severe teeth wear. Complete mouth rehabilitation was planned to restore esthetics and function. Due to the presence of a subgingival finish line, poor fit of the margin of the temporary crown, and insufficient oral hygiene management, gingival overgrowth and bleeding occurred in many teeth, and inaccurate impressions were taken. After dividing each arch and taking partial impressions, a transfer coping was made using pattern resin on each working model die, and after being placed on the abutment teeth, a master cast was made through pick-up impression taking to produce prosthesis. In this case, the final prosthesis produced on the second master cast were fitted in terms of proximal contact, marginal fit, vertical occlusal dimension, facial appearance, esthetics, and occlusion. After complete mouth rehabilitation, the patient was satisfied with function and esthetic appearance.

• Journal of Veterinary Clinics
• /
• v.30 no.6
• /
• pp.478-481
• /
• 2013

A mass was detected in the oral cavity from a 18-year-old female miniature poodle dog. Grossly, the mass was soft to hard, red to purple, and $1.5{\times}1.5{\times}1cm$ in size. Histopathologically, the mass was composed of hyperplastic gingival epithelium, well-vascularized stroma, closely packed pleomorphic cells, and numerous giant cells with multiple nuclei and abundant eosinophilic cytoplasm. Immunohistochemically, tumor cells were positive for alkaline phosphatase and cytokeratin 7, but not positive for CD68 unlike in human. The mass was diagnosed to peripheral giant cell granuloma in oral cavity through typical clinical and histopathological features.

• Journal of Periodontal and Implant Science
• /
• v.25 no.1
• /
• pp.14-23
• /
• 1995

Cyclosporin A is a powerful immunosuppressive agent commonly used for patients receiving organ transplants. Like phenytoin and the calcium channel blockers, the drug is associated with gingival overgrowth. The purpose of this study was to compare the correlation with gingival overgrowth score and clinical indices(i.e, : plaque index, papillary bleeding index, probing depth) and correlation with gingival overgrowth score and microorganism distribution in use of phase contrast microscope. After renal tranplant, taking cyclosporin A 40 patients participating in this investigation. Post - transplatation cyclosporin medication period was average $17.53{\pm}15.75$ months. In previous study reported that gingival overgrowth is an adverse side - effects seen in about 25-81% of patient taking cyclosporin A. The results were as follows : 1. Gingival overgrowth prevalence in taking cyclosporin A patients was 77.5%. Prevalence rate of region was anterior region(26 teeth, 55.3%), molar region(14 teeth, 29.8%), premolar region(7 teeth, 14.8%) in turns. Gingival overgrowth score by Angelopoulos & Goaz method was molar region($1.56{\pm}0.81$), anterior region($1.52{\pm}0.75$), premolar region($1.14{\pm}0.90$) in turns. 2. Medication period was not correlation with gingival overgrowth score. 3. Clinical indices and gingival overgrowth score were as follows. 1) Plaque index and gingival overgrowth score was significantly correlated(p

• Journal of Periodontal and Implant Science
• /
• v.30 no.3
• /
• pp.609-619
• /
• 2000

Cyclosporin A(CsA) is now widely used to treat organ transplant recipients. But CsA has various short-and long-term side effects. Especially, gingival hyperplasia is not easy to resolve since its nature is still unknown. This study discusses the pathogenesis of CsA-induced gingival hyperplasia on the basis of data obtained from light and electron microscopic studies of biopsis from patients on CsA treatment after kidney transplantation. Light microscopically, the multilayered squamous epithelium showed an irregular surface of parakeratosis and deep invaginations in the subepithelial tissue. At lamina propria, we observed bundles of irregularly arranged collagen fiber, some fibroblasts, numerous capillary vessels and a large diffuse infiltration of plasma cells. Ultrastructurally, many fibroblasts, collagen fibers, collagen fibrils were present in lamina propria. On the basis of the data collected, we propose that the morphological features of the dimensional increase in gingival tissue associated with CsA treatment in kidney transplant patients may be considered proliferative fibroblasts, collagen fibers, collagen fibrils in lamina propria.

• Journal of Periodontal and Implant Science
• /
• v.26 no.3
• /
• pp.715-724
• /
• 1996

This study was performed to identify the proliferation and to measure the alteration of alkaline phosphatase activity in human gingival fibroblasts cultured. For the present study, the authors cultured the human gingival fibroblasts oriented from the sound interdental gingiva, and used third passage. It was used methyl $[^3H]$ Thymidine to identify the proliferation in human gingival fibroblasts and used 410nm of the spectrophotometer to measure the alteration of the alkaline phosphatase activity in human gingival fibroblasts. The results were as follows: 1. There was a statistically significant increase in the proliferation of gingival fibroblasts following low level laser irradiation at 24 hour(p<0.05). 2. There was a statistically significant increase in activity of alkaline phosphatase compared to control group at 5-day laser irradiation after in laser irradiation groups(p<0.05). And there was a statistically significant increase in activity of alkaline phosphatase compared to control group at 7-day laser irradiation after in the I-minute laser irradiation group(p<0.05), but there was a statistically significant decrease in activity of alkaline phosphatase compared to 1minute laser irradiation group at 7-day laser irradiation in the 2-minute laser irradiation group after(p<0.05). The results, within the limits of the present experiments, suggest that, the low level laser irradiation accelerates the proliferation of gingival fibroblasts and alters the alkaline phosphatase activity until the restricted period.

• Journal of Periodontal and Implant Science
• /
• v.24 no.2
• /
• pp.357-375
• /
• 1994

The purpose of this study was to investigate the differences of histochemical characteristics in inflammatory fibrous gingival hyperplasia (FGH), phenytoin-induced gingival hyperplasia(PIGH), idiopathic gingival hyperplasia(IDGH) and control groups (healthy and inflammatory gingiva) by immunohistochemical method with various antibodies and histomorphological analysis. In immunohistochemical finding, antibodies to inflammatory cells (T/B lymphocytes, macrophages, other monocytes), proliferating cell nuclear antigen(PCNA), epidermal growth factor(EGF), factor VIII, and type I collagen were used. 1. The inflammatory infiltrates in FGH were less than those in inflammatory gingiva. The composition of inflammatory cells of PIGH was similar with that of FGH. IDGH showed a similar histologic findings with healthy gingival tissue. 2. In FGH, the number of fibroblasts and newly-formed collagen fibers was increased. No significant increase of fibroblasts and the dense accumulation of thick collagen fibers were seen in PIGH. The increase of fibroblasts and the dense accumulation of thick collagen were seen in IDGH. 3. PCNA-positive cells were localized mainly in the area accumulated with inflammatory cells and blood vessels, significantly increased in all hyperplastic tissue groups, and distributed evenly in IDGH. 4. The distribution of EGF were not observed in healthy gingiva but detected locally in area with confluent blood vessels,without significant difference between the other tissue groups. This results suggest that inflammation plays a significant role in inducing hyperplastic change of gingival tissue. While in DIGH, drug itself as well as inflammation seems to attribute to hyperplastic change.