• Title/Summary/Keyword: 치아탈회

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DEVELOPING OF QLF-D FOR EARLY DETECTION OF DENTAL CARIES (치아 우식증의 조기 진단을 위한 QLF-D 개발)

  • Park, Hyung-Ju;Kim, Jong-Soo;Yoo, Seung-Hoon;Shin, Ju-Sun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.38 no.4
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    • pp.317-326
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    • 2011
  • QLF-D system composed with DSLR(digital single-lens reflex) camera, and the images of natural enamel caries and artificial caries was developed from 2 days to 14 days captured by QLF-D system. The correlation between lesion depth of the polarized microscope and luminosity ratio of QLF-D image was analyzed and the results were summarized as follows: 1. The Pearson correlation value between the lesion depth of polarized microscope images and luminosity ratio of QLF-D images was 0.969(p<0.01). 2. From Regression analysis of lesion depth from polarized image by demineralized period, the equation was y = 8.67x - 1.16(p<0.05). 3. From Regression analysis of luminosity ratio from QLF-D image by demineralized period, the equation was y = 3.53x + 6.42(p<0.05). From the results, QLF-D system can detect the enamel caries at the very early stage and can monitor the progression of demineralization and remineralization. For the convenient use of QLF-D system in the laboratory, the image analysing software was needed to analyze of interest site of enamel caries lesion.

THE REMINERALIZATION ASPECT OF ENAMEL ACCORDING TO CHANGE OF THE DEGREE OF SATURATION OF THE ORGANIC ACID BUFFERING SOLUTION IN pH 5.5 (pH 5.5에서 유기산 완충용액의 포화도 변화에 따른 법랑질의 재광화 양상)

  • Park, Jin-Sung;Park, Sung-Ho;Park, Jeong-Won;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.35 no.2
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    • pp.96-105
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    • 2010
  • The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (${\times}100$). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were est mated. While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.

Effects of 3.5% Hydrogen Peroxide Containing Dicalcium Phosphate Dihydrate on the Tooth Enamel Surface (Dicalcium Phosphate Dihydrate를 함유한 3.5% 과산화수소가 치아표면에 미치는 영향)

  • Shim, Youn-Soo
    • Journal of dental hygiene science
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    • v.12 no.4
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    • pp.320-328
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    • 2012
  • The purpose of this study was to evaluate the tooth whitening and properties of an enamel surface after treatments with tooth bleaching agents that contained dicalcium phosphate dihydrate (DCPD) and hydrogen peroxide (HP). Thirty specimens were obtained from fifteen premolar and were randomly divided into three groups (n=10): 1, 3.5% HP + 0 g DCPD; 2, 3.5% HP + 0.1 g DCPD; 3. 3.5% HP + 1 g DCPD. All groups were bleached 8 hours per day for 14 days. With increasing DCPD concentration, the pH values in the agents increased, making it less acidic. However, there was no statistically significant difference (p>.05). As the concentration of DCPD was increased, the concentration of Ca and P was also increased. In all groups, after the tooth whitening, the tooth color was found to have a value of $L^*$ (p<.05). All groups showed significantly decreased enamel microhardness compared to their baseline (p<.05). The percentage microhardness loss (PML) of the group A1 and A2 were significantly lower than that of group A3. The obvious variation of morphology was observed on enamel surfaces in group A1. Following an analysis of the constituents of enamel surface after bleaching, as DCPD content was increased, the amount of Ca and P was increased. In this study, the experimental results suggest that DCPD/HP agent less demineralization changes such as the erosion morphology and hardness loss without compromising whitening efficiency.

The effects of fluoride releasing orthodontic sealants on the prevention and the progressive inhibition of enamel demiheralization in vitro (광중합형 및 자가중합형 교정용 전색제의 치아우식예방 및 진행억제효과에 관한 실험적 연구)

  • Chae, Seung-Won;Cho, Jae-O;Yoon, Young-Jooh;Kim, Kwang-Won
    • The korean journal of orthodontics
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    • v.27 no.6 s.65
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    • pp.979-995
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    • 1997
  • The purpose of this study was to identify the preventive and the progressive inhibitory effects of enamel demineralization with fluoride releasing light-and self-cured orthodontic sealants(FluoroBond), in vitro, under the polarizing light microscope and the scanning electon microscope. The polarizing light microscopic group was subdivided into seven groups(Group A-Group G). The scanning electron microscopic group was also subdivided into seven groups(Group A'-Goup G'). For polarizing light microscopic evaluation, longitudinal sections were made longitudinally by Maruto cutter(Maruto Co., Japan) and Maruto grinding machine(Maruto Co., Japan). Sections were examined and photographed by the polarizing light microscope(Olympus Optical Co., Japan) using crossed polars and with the enamel rod longitudinal axis oriented at $45^{\circ}$ to the extinction position. For scanning electron microscopic evaluation, the specimens were coated with a highly conducting layer of gold palladium in a model Hus-4 high-vacuum evaporator and examined in an ISI-100B scanning electron microcope operated at 20kV. The results of this study were as follows : 1. The mean depths of artificial carious lesions under a polarized light microscope were $Group\;A(5.08{\mu}m),\;Group\;B(47.82{\mu}m,\;Group\;C(8.42{\mu}m),\;Group\;D(7.20{\mu}m),\;Group\;E(85.41{\mu}m),\;Group\;F(60.38{\mu}m),\;Group\;G(60.13{\mu}m)$. 2. There were statistically significant differences in Group B compared with Group A, C, and D(p<0.05), and also, in Group I compared with Group F and Group G(p<0.05). 3. Light-and self-cured orthodontic sealants had the preventive effects of enamel demineralization. 4. Light-and self-cured orthodontic sealants had the progressive inhibitory effects of enamel demineralization. 5. The time progress of demineralizing agent had no influence on the samples of light-and self-cured orthodontic sealants under the scanning electron microscope. 6. There was no difference between the specimens of light-and self-cured orthodontic sealants both in the polarized light microscopic group and in the scanning electron microscopic group.

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Initial changes of dental plaque, gingivitis and decalcification in Korean orthodontic patients with fixed appliance (한국인 고정식 교정 환자의 치태, 치은염 및 탈회의 초기 변화에 관한 연구)

  • Kang, Kook-Jin;Shon, Byung-Hwa
    • The korean journal of orthodontics
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    • v.29 no.3 s.74
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    • pp.361-374
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    • 1999
  • Intraoral filled type of orthodontic appliance can cause reversible or irreversible damages such as gingivitis, periodontitis, enamel decalcification, dental caries, root resorption, and pulpal changes. Such adverse effects are brought by increase in dental plaque as well as oral flora. Such an increase causes gingival inflammation and enamel decalcification. The purpose of this study is to get klowledge on initial changes in dental plaque, gingivitis, and enamel decalcification after bonding fixed orthodontic appliances according to time flow, gender, and sides(right/left) of premolar region. For control group, 48 students of dental college, Yonsei university(26 males, 22 females) were chosen; for experimental group, 73 orthodontic patients(36 males, 37 females) who will be treated with fixed appliances were chosen. All the subjects had no systemic disease, juvenile periodontitis and all the females had passed their ,menarche. Tooth brushing instruction was given to all the subjects prior to the experiment. For control group, plaque index, gingival index, and decalcification index were measured twice at 3 weeks interval ; for experimental group, the same was done prior to, 3, 6, 9 weeks after bonding fixed appliances. The following results were obtained: 1. In plaque index 3 weeks after placement of appliances, and it showed gradual increase afterwards. 2. In gingival index3 weeks after placement of appliances, and afterwards it showed increase at a faster rate than plaque index. 3. Enamel decalcification began to show between 3 and 6 weeks after bonding fixed appliances. Decalcification index began to increase 6 weeks after appliance placement, but there was no statistical significance. 4. When the comparison was made between two sides of premolar region, the right side showed greater index in plaque and gingival index of experimental group.

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THE EFFECT OF THE pH OF REMINERALIZED BUFFER SOLUTIONS ON DENTIN REMINERALIZXATION (재광화 완충용액의 pH 변화가 상아질의 재광화에 미치는 영향)

  • Kim, Sung-Chul;Roh, Bung-Duk;Jung, Il-Young;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.32 no.2
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    • pp.151-161
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    • 2007
  • Dental caries is the most common disease in the oral cavity However, the mechanism and treatment of dental caries is not completely understood since many complex factors are involved. Especially the effect of pH on remineralization of early stage of dental caries is still controversial In this study, dental caries in dentin was induced by using lactic acidulated buffering solutions and the loss or inorganic substance was measured. Also decalcified specimens were remineralized by three groups of solution with different pH (group of pH 4.3, 5.0, and 5.5). Then, the amount and the area of inorganic substance precipitation was quantitatively analyzed with microradiograph. Also a qualitative comparison of the normal phase the demineralized phase, and the remineralized phase of hydroxyapatite crystal was made under SEM. The results were as follows, 1. In microradiograghic analysis, as the pH increased, the amount of remineralization in decalcified dentin tended to increase significantly As the pH decreaced, deeper decalcification, however, occurred along with remineralization. The group of pH 5.5 had a tendency to be remineralized without demineralization (p<0.05). 2. In SEM view, the remineralization in dentine caries occurred from the hydroxyapatite crystal surface surrounding the mesh of organic matrix, and eventually filled up the demineralized area. 3. 5 days after remineralization, hydroxyapatite crystal grew bigger with deposition of inorganic substance in pH 4.3 and 5.0 group, and the crystal in the remineralized area appeared to return to normal. After 10 days, the crystals in group of pH 4.3 and 5.0, which grew bigger after 5 days of remineralization, turned back to their normal size, but in group of pH 5.5, some crystals were found to double their size. In according to the results of this experiment, the decalcifying and remineralizing process of dentine is neither simple nor independent, but a dynamic process in which decalcification and remineralization occur simultaneously. The remineralization process occurred from the hydroxyapatite crystal surface.

Direct detection of cariogenic streptococci in metal brackets in vivo using polymerase chain reaction (교정용 메탈 브라켓에서 자가중합효소연쇄반응을 통한 치아우식증 원인균의 탈출)

  • Ahn, Sug-Joon;Lee, Shin-Jae;Baek, Seung-Hak;Kim, Tae-Woo;Chang, Young-Il;Nam, Dong-Seok;Lim, Bum-Soon
    • The korean journal of orthodontics
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    • v.35 no.4 s.111
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    • pp.312-319
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    • 2005
  • Streptococcus mutans and Streptococcus sobrinus are major etiological agents in enamel demineralization around orthodontic appliances. This study was designed to examine the prevalence of these streptococci on orthodontic brackets in vivo using polymerase chain reaction. Four incisor brackets in the upper and lower arches were removed and collected from 80 patients at the time of debonding. The genomic DMA of adhered bacteria was extracted and each dextranase gene of S. mutans and S. sobrinus was amplified using the specific oligonucleotide primers. The results showed that the maxillary incisor brackets were colonized by both cariogenic streptococci to a somewhat higher degree than that taken from the mandible. The prevalence of S. mutans was $50.0\%$ on the maxillary incisor brackets and $33.8\%$ on the mandibular incisor brackets, and that of S. sobrinus was $17.5\%$ and $15.0\%$, respectively. Both species were detected on the maxillary incisor brackets of 7 patients $(8.8\%)$ and the mandibular incisor brackets of 5 patients $(6.3\%)$. These results suggest that cariogenic streptococci can adhere to the incisor brackets and may be resident species on the incisor brackets.

Quantitative analysis of mutans streptococci adhesion to various orthodontic bracket materials in vivo (다양한 교정용 브라켓 원재료에 부착하는 mutans streptococci 양의 비교분석)

  • Yu, Jin-Kyoung;Ahn, Sug-Joon;Lee, Shin-Jae;Chang, Young-Il
    • The korean journal of orthodontics
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    • v.39 no.2
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    • pp.105-111
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    • 2009
  • Objective: To estimate the effects of bracket material type on enamel decalcification during orthodontic treatment, this study analyzed the adhesion level of mutans streptococci (MS) to orthodontic bracket materials in vivo. Methods: Three different types of orthodontic bracket materials were used: stainless steel, monocrystalline sapphire, and polycrystalline alumina. A balanced complete block design was used to exclude the effect of positional variation of bracket materials in the oral cavity. Three types of plastic individual trays were made and one subject placed the tray in the mouth for 12 hours. Then, the attached bacteria were isolated and incubated on a mitis salivarius media containing bacitracin for 48 hours. Finally, the number of colony forming units of MS was counted. The experiments were independently performed 5 times with each of the 3 trays, resulting in a total of 15 times. Mixed model ANOVA was used to compare the adhesion amount of MS. Results: There was no difference in colony forming units among the bracket materials irrespective of jaw and tooth position. Conclusions: This study suggested that the result of quantitative analysis of MS adhesion to various orthodontic bracket materials in vivo may differ from that of the condition in vitro.

MORPHOLOGICAL STUDY ON PERIODONTAL REGENERATION AND ROOT RESORPTION FOLLOWING TOOTH REIMPLANTATION IN RATS (쥐 치아 재식후 나타나는 치주조직의 치유와 치근흡수에 관한 형태학적 연구)

  • Lee, Seung-Jong;Matsuura, Masahiro;Lin, Wen-Lang;Cho, Moon-Il
    • Restorative Dentistry and Endodontics
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    • v.24 no.1
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    • pp.116-128
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    • 1999
  • 치근흡수 연구에서 치아재식을 위한 적당한 동물모델을 찾는일은 대단히 중요하다. 본 실험에서는 쥐 대구치의 발치 및 재식을 통해서 치근흡수가 발현되는 모양을 관찰했다. 모두 20마리의 쥐(30일생)에게 0.4% ${\beta}$-APN(aminoproprionitrile)가 포함된 분말용쥐사료를 5일간 먹인후 케타민 마취후 상악좌우측 제일대구치를 손상을 최소화하여 발치 하였다. 발거된 쥐치아는 0.02M Tris-HCl로 표면처리하여 부착치근막을 제거한후 파라핀팍스로 치근단을 밀폐 하여 재식하였다. 재식후 1, 2, 4, 6, 8 및 12일이 경과된 후 심장관혈을 통하여 쥐를 희생시킨후 3% glutaraldehyde가 포함된 EDTA에서 탈회한후 Epon 포매 하였다. 모든 시편은 유리칼을 사용하여 1미크론 두께로 절편한후 veronal acetate buffer에 녹인 1% toluidine blue로 염색하였다. 파치세포양 세포에 의한 치근흡수는 재식 5-7일후 처음 관찰되었다. 재식 12일후에는 치근 전체에 걸친 광범위한 치근흡수가 관찰되었다. 이상의 결과 ${\beta}$-APN을 포함한 쥐사료 식이요법은 쥐 상악제일대구치의 발치와 재식을 용이하게 하여 일정한 치근흡수를 발현시키는데 많은 도움이 된 것으로 판단되었다.

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THE REMINERALIZING FEATURES OF PH 5.5 SOLUTIONS OF DIFFERENT DECREE OF SATURATIONS ON ARTIFICIALLY DEMINERALIZED ENAMEL (pH 5.5에서 재광화 용액의 포화도 변화가 인공 탈회된 법랑질의 재광화에 미치는 영향)

  • Kwak, Young-Jun;Kim, Eui-Seoug;Park, Sung-Ho;Gong, Hyung-Kyu;Lee, Yoon;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.33 no.5
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    • pp.481-492
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    • 2008
  • The purpose of this study is to observe and compare the remineralization tendencies of artificially demineralized enamel by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 36 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 3 days in order to produce dental caries artificially that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffered remineralization solution of three different degrees of saturation (0.25, 0.30, 0.35) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope $({\times}100)$. The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were determined. In conclusion, in the group with the lowest degree of saturation, remineralization occurred thoroughly from the surface to the subsurface lesion, whereas in the groups with greater degree of saturation showed no significant change in the subsurface lesion, although there was corresponding increase in the remineralization width on the surface zones.