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Direct detection of cariogenic streptococci in metal brackets in vivo using polymerase chain reaction  

Ahn, Sug-Joon (Department of Orthodontics, School of Dentistry and Dental Research Institute, Seoul National University)
Lee, Shin-Jae (Department of Orthodontics, School of Dentistry and Dental Research Institute, Seoul National University)
Baek, Seung-Hak (Department of Orthodontics, School of Dentistry and Dental Research Institute, Seoul National University)
Kim, Tae-Woo (Department of Orthodontics, School of Dentistry and Dental Research Institute, Seoul National University)
Chang, Young-Il (Department of Orthodontics, School of Dentistry and Dental Research Institute, Seoul National University)
Nam, Dong-Seok (Department of Orthodontics, School of Dentistry and Dental Research Institute, Seoul National University)
Lim, Bum-Soon (Department of Dental Biomaterials Science, School of Dentistry and Dental Research Instittue, Seoul National University)
Publication Information
The korean journal of orthodontics / v.35, no.4, 2005 , pp. 312-319 More about this Journal
Abstract
Streptococcus mutans and Streptococcus sobrinus are major etiological agents in enamel demineralization around orthodontic appliances. This study was designed to examine the prevalence of these streptococci on orthodontic brackets in vivo using polymerase chain reaction. Four incisor brackets in the upper and lower arches were removed and collected from 80 patients at the time of debonding. The genomic DMA of adhered bacteria was extracted and each dextranase gene of S. mutans and S. sobrinus was amplified using the specific oligonucleotide primers. The results showed that the maxillary incisor brackets were colonized by both cariogenic streptococci to a somewhat higher degree than that taken from the mandible. The prevalence of S. mutans was $50.0\%$ on the maxillary incisor brackets and $33.8\%$ on the mandibular incisor brackets, and that of S. sobrinus was $17.5\%$ and $15.0\%$, respectively. Both species were detected on the maxillary incisor brackets of 7 patients $(8.8\%)$ and the mandibular incisor brackets of 5 patients $(6.3\%)$. These results suggest that cariogenic streptococci can adhere to the incisor brackets and may be resident species on the incisor brackets.
Keywords
Cariogenic streptococci; Detection; Metal bracket; Polymerase chain reaction;
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