• Journal of fish pathology
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• v.18 no.1
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• pp.29-37
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• 2005

This study investigated external symptoms, behavior characteristics and the distribution pattern of the scuticociliates in the skin, gill and blood vessel of the tiger puffer, Takifugu rubripes, infected with scuticociliates among tiger puffer cultured. The fish infected with scuticociliates did not show any external symptoms, such as change in body color or swimming behavior, in the early infection stage. However, they showed congestion, erosion, and ulcer on the skin and fin at advanced stages. They showed a turning movement, mainly stayed on the bottom, and swam with their mouths open at the surface of water. Some fish showed a sudden swimming movement of zigzag type. The scuticociliates were ovoid in shape and $20\times40{\mu}m$ in size. The scuticociliates had a contractile vesicle and a caudal cilium that ranged 10-12 ${\mu}m$, and reproduced by binary fission. Therefore the scuticociliates were identified as ciliated, belonging to class Hymenostomatia, order Scuticociliatida in morphogenetic character. The gills infiltrated with scuticociliates showed clubbed gill filament due to hypertrophy of gill lamella. Within the blood vessels, scuticociliates were observed one to ten individuals, depending on diameter of the blood vessels. Some of the scuticociliates were observed to have ingested erythrocytes.

• Korean Journal of Veterinary Research
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• v.41 no.2
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• pp.211-218
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• 2001

The $M_r$ 63,000 glycoprotein (GP63) and lipophosphoglycan (LPG) of Leishmania donovani were evaluated as vaccine candidates against visceral leishmaniasis. Mice were immunized with liposomeencapsulated GP63 and/or LPG that were purified from the soluble extract of L. donovani promastigotes, and were challenged with virulent amastigotes. Mice immunized with GP63/LPG in liposomes plus BCG resulted in a 27.4% reduction of amastigotes in the liver compared to the control group (liposomes plus BCG), and mice immunized with liposome-GP63 plus BCG failed to induce a protective immune response against the challenge infection. Immunization of mice with GP63 fused to the Schistosoma japonicum glutathione S-transferase (GP63-GST) plus BCG also failed to elicit protective immunity. To analyze the cause of failure to induce protection, cytokine release from the spleen cells of immunized mice and Leishmania-specific serum antibodies were analyzed. Spleen cells from mice immunized with GP63-GST plus BCG that were exposed to soluble extract of L. donovani in vitro produced 10-fold greater quantities of IFN-gamma and 3-fold greater quantities of IL-5 than cells from mice receiving BCG only or saline. Western blot analysis revealed that sera from these mice had Leishmania-specific antibodies recognizing 1 to 3 antigens of L. donovani with M. W. of 60-65 kDa. Although immunization of mice with GP63-GST induced a strong Th1 response, this study indicated that GP63-GST simultaneously elicited the Th2 response of the CD4+ T-cell, which was known to abrogate the protective immune response conferred by the Th1 effector function.

• Transactions of the Korean hydrogen and new energy society
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• v.14 no.3
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• pp.217-228
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• 2003

Li 이차 전지 음극용 활물질인 $Co_3O_4$의 초기 충방전 효율을 향상시키기 위해 chemical reduction method나 mechanical milling법을 이용하여 $Co_3O_4$에 Ni을 첨가하여 $Ni-Co_3O_4$, 복합물을 제조하였다. 그 결과 초기 충방전 효율이 약 69%에 불과한 $Co_3O_4$에 비해 mechanical milling법을 이용하여 제조된 $Ni-Co_3O_4$ 복합물은 약 79%이상의 대폭 향상된 초기 충방전 효율 특성을 나타내었다. 하지만 chemical reduction method를 이용하여 제조된 $Ni-Co_3O_4$ 복합물은 약 71%의 초기 충방전 효율 특성만을 나타내었다. SEM 분석을 통해 각 물질의 표면 형상을 관찰한 결과 mechanical milling법과 chemical reduction method를 통해 제조된 $Ni-Co_3O_4$ 복합물에서는 $Co_3O_4$ 표면에 분포된 Ni의 균일성의 차이가 존재하였다. $Co_3O_4$가 $Li_2O$의 분해, 형성에 의해 충방전되고 Ni이 $Li_2O$의 분해를 촉진시키는 효과를 가지고 있음을 고려할 때 이러한 균일성의 차이는 결국 Ni 과 $Co_3O_4$ 사이의 접촉면적의 차이로 이어져 $Ni-Co_3O_4$ 복합물의 초기 충방전 효율 특성이 그 제조 방법에 따라 달라지는 것으로 보인다.

• Journal of the Korean earth science society
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• v.30 no.4
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• pp.409-426
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• 2009

In the four sedimentary cores from Yeoja Bay, the analyses of grain size, benthic foraminiferal species compositions, assemblages and statistics were carried out to investigate the effects of late Holocene sea-level change on benthic foraminifera. The core sediments were mainly composed of fine-grained silt and clay. The benthic foraminifera were classified into 27 species of 16 genera, 30 species of 21 genera, 50 species of 29 genera and 52 species of 29 genera in Core YC-1 to 4, respectively. In the result of cluster analysis, it seemed that Group 1 (Core YC-1 and 2) of representative A. beccarii assemblages was deposited in upper bay environment and Group 2 (Core YC-3 and 4) of representative E. clavatum-A. beccarii assemblages was deposited in inner bay environment affected by offshore water. In the result of species composition analysis, the production frequency of A. beccarii was gradually decreased from low layer to upper layer, whereas production frequency of E. clavatum and P.F./T.F. was gradually increased to upward. These change patterns appeared in benthic and planktonic foraminifera seemed to reflect the late Holocene sea-level rise in Yeoja Bay.

• The Journal of the Korea Contents Association
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• v.21 no.11
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• pp.243-256
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• 2021

is a film that lifted Director Joon Ho Bong and Korea to the world-class level. A number of researchers have released findings of studies on based on their own interests and perspectives. The current study discussed the historical facts of black comedy in film and how black comedy from play has been used for epic characteristics and formal measures, although it was not a specific rule, through the perspectives of contemporary writers, such as Beckett, Ionesco, Durrenmatt, and Brecht. Some tools used for comedies and tragedies shared the same concept with black comedy in film and black comedy was a unique characteristic of film. The study analyzed how black comedy was signified in for paradox and nonalignment of purpose/intent and consequences as epic characteristics and parody, grotesque, and coincidence as formal measures. It also discussed the meaning of Director Bong's black comedy in and how the audience accepted it. Both the contemporary writers and Director Bong has a compassionate perspective, but the his was different from the work of other contemporary writers. Therefore, the study discussed his creativity behind .

• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.356-362
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• 2022

Research related to hydrogen technology is being actively conducted around the world. Korea is also making great efforts to develop technology to leap forward as a hydrogen economy powerhouse. In particular, the world's No. 1 hydrogen vehicle penetration rate is proof of this. However, the construction of hydrogen refueling stations is being delayed. The biggest delay factor is the public opposition. As such, policies without public support cannot be successfully implemented and are not sustainable. Therefore, this study intends to analyze the factors affecting the acceptability of hydrogen refueling stations in favor of and against them. As a research method, the basic factors affecting acceptability were identified by reviewing previous studies, and a questionnaire was designed and investigated based on the established factors. The validity and reliability of the questionnaire were verified, and the hypothesis was verified through correlation analysis. And, using structural equation modeling, a factor model was developed on the acceptability of hydrogen refueling stations. As a result of the study, acceptability defined private acceptability and public acceptability. In the case of private acceptability, it was confirmed that the higher the attitude toward the environment, the higher the level of knowledge about the hydrogen charging station, and the lower the degree of feeling the risk of the hydrogen charging station, the higher the acceptability. In the case of public acceptability, it was confirmed that the higher the benefit, the better the attitude toward the environment, and the lower the risk-taking characteristics of the individual, the higher the acceptability. Therefore, in this study, based on the potential factors verified in previous studies, the main factors affecting the acceptance on hydrogen refueling stations were identified. And the acceptance model was developed using structural equation modeling. This study is expected to provide basic data to seek ways to improve the acceptance of public when implementing national policies such as hydrogen refueling stations, and to be used analysis data for scientific communication.

• Korean journal of applied entomology
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• v.62 no.2
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• pp.69-78
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• 2023

The currently implemented Positive List System, sales manager training, and pesticide sales record systems focus on the safety of determined pesticide use, but the proposed agrochemical pharmacist (AP) system aims accurate diagnosis and reasonable prescription. In this study, 81% of famers claimed expert assistance for purchasing and applying pesticides, and 42~86% of sales managers insisted on the strengthening expertise in diagnosis and prescription. In the institutionalization of AP, a special implementation guideline is essential to embrace the present sales managers with long experience. Sales managers of commercial and agricultural cooperatives recognize the qualifications of agrochemical pharmacists as a strategy to differentiate themselves from competitors, and increase sales through market positioning of "pesticides prescribed by trustworthy agrochemical pharmacists", not sales managers anymore.

• Journal of the Korean Society of Radiology
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• v.81 no.1
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• pp.135-146
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• 2020

Purpose To describe magnetic resonance imaging (MRI) findings in 10 cases of Toxocara canis myelitis and to analyze these findings to aid in the diagnosis of this condition. Materials and Methods From June 2015 to March 2018, we retrospectively analyzed the electronic medical records and MR images of patients who were diagnosed with Toxocara canis myelitis. The analysis of the MR images was based on a discussion between an experienced spinal radiologist and a radiology resident. Results This study classified MRI findings into the following two types. Type 1 was defined as central and diffuse T2 high signal intensity on the axial plane, which was observed in 50% of all cases. All lesions showed avid enhancement, mostly in the posterolateral or posterior region (4 cases, 80%). Type 2 was defined as wedge-shaped or focal T2 high signal intensity in the posterolateral or posterior region and corresponded to the remaining 50% of the cases. In this case, the extent of the lesion was relatively small and contrast enhancement was observed in only one case. Conclusion This study revealed that various imaging findings could be observed in cases of Toxocara canis myelitis, and the findings were classified into two types.

• Parasites, Hosts and Diseases
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• v.24 no.1
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• pp.3-141
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• 1986

간흡충은 우리나라를 비롯하여 중국, 대만, 일본 및 월남등지에 널리 분포되어 있으며 1947년에 Stoll씨의 통계에 의하면 세계적으로 1,900만명이 넘는 감염자가 있다고 하였다. 현재 우리나라에서만 약 200만명의 간흡충 감염자가 있을 것으로 추산되고 있다. 간흡충은 McConnell이 1874년에 인도의 Calcutta에서 한 중국인의 간담도에서 본충을 처음 발견한 것이다. 이것을 Cobbold(1875)가 Distoma sinense라고 명명하였다가 후에 Looss (1907)가 Clonorchis sinensis라고 하였다. Kobayashi (1910)는 담수어가 간흡충감염의 중간숙주로 작용한다는 것을 처음으로 발견하였고 Muto (1918)에 의하여 왜우렁이가 제1중간숙주임을 발견함으로서 간흉충의 생활사가 확실해 졌다. Faust 및 Khaw (1927)에 의하여 중국에서 간흡충의 생물학 및 역학에 관한 광범한 연구업적이 발표된 이래 일본, 한국, 중국등 여러곳에서 많은 사람들에 의하여 연구되어 왔다. 그러나 적절한 치료방법이 없었고 또 민물고기를 생식하는 식습관과 위생시설의 미비등 여러가지 요인때문에 예방이 어려움에 직면하였다. 최근 다행히 새로이 개 발된 praziquantel이 간흡충중에 대하여 우수한 치료효과를 나타내고 집단치료에도 적합하다는 것이 증명됨으로서 간흡충증 예방과 관리에 큰 서광이 보이게 되었다. 따라서 간흉충증의 발생과 임상적 병증의 중요성을 좀 더 이해하기 위하여 간흡충체의 미세구조, 병인, 역학, 임상증상등 최근 알려진 새로운 지견과 우리나라에 있어서 간흡충증의 분포 및 유행지 형성 상황을 수학적으로 역학적 분석을 시도하였다. 그리고 과거 간흡충중의 화학치료에 관한 연구성적을 종합하여 화학요법제의 특성 빚 치료 적용여부에 대하여 논하였고 각약제의 시험관내 및 실험동물에 있어서의 살충효과와 임상시험에 있어서의 치료효과 및 부작용에 대하여 기술하였다. 그리고 새로 개발된 praziquantel의 간흡충에 대한 작용기전에 대하여도 논하였다. (중략)

• Parasites, Hosts and Diseases
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• v.21 no.1
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• pp.32-39
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• 1983

실험동물에서 짧은 기간 감염시킨 후 얻은 간흡충(Clonorchis sinensis)에서는 거의 볼 수 없지만, 간, 담도 수술 또는 부검을 실시할 때 사람에서 얻은 간흡충중에는 검은 색소를 갖는 것을 관찰할 때가 있다. 이 색소는 살아 있는 간흡충에서도 명백하며, 색소가 없는 간흡충은 색이 분홍빛이고 투명한데 비하여 색소가 있는 것은 검은색을 띠고 불투명하다. 사람에서 얻은 간홉충 모두가 검은 색소를 갖고 있는 것은 아니며, 이제까지의 경험으로는 30세 이상 어른에서 얻은 간흡충중 그 길이가 약 1cm이상인 것에서 나타난다. 이 색소의 성분에 대하여 과거에는 빌리루빈, 또는 숙주 혈구세포에서 유래한 철을 포함하는 색소일 것이라고 생각하고 있었다. 저자등은 이 색소가 간흡충의 노쇠현상과 관련된 것으로 생각하고, 그렇다면 lipofuscin일 것이라고 생각하였다. 위의 가정을 확인하기 위하여 육안적으로 분명히 검은 색소가 있은 충체의 조직절편을 만들고, H & E, prussian blue, bilirubin, Fontina-Masson, PAS, amylase-PAS, Ziehi-Neelsen 및 AFIP method for lipofuscin등 8가지 조직화학적 염색을 실시하였다. 색소는 H & E, prussian blue, bilirubin, montana-Masson염색으로 착색되지 않았으며, PAS, amylase-PAS, Ziehi-Neelen, 및 AFIP method for lipofuscin에 염색되어 이것이 조직화학적으로 리포푸신 색소임을 확인하였다. 색소를 다시 전자현미경으로 관찰한 바 색소과립안에는 모양이 등글거나 타원형이고 크기가 $0.05~0.240{\mu}m$인 세포막구조가 산재하였음을 발견할 수 있어 간흡충의 검은 색소는 리포푸신 색소임을 다시 확인할 수 있었다.