• Title/Summary/Keyword: 출현율

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QUANTITATIVE DETECTION OF PERIODONTOPATHIC BACTERIA USING REAL-TIME PCR (Real-time PCR을 이용한 치주질환 원인균의 정량적 분석)

  • Kim, Seon-Mi;Yang, Kyu-Ho;Choi, Nam-Ki;Kang, Mi-Sun;Oh, Jong-Suk
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.3
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    • pp.494-503
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    • 2008
  • Real-time PCR 기법을 이용하여 구강내 치태에서 특정 세균의 존재여부를 알 수 있을 뿐 아니라 정량적 분석이 가능하게 되었다. 이 연구는 real-time PCR 기법을 이용하여 8-18세의 청소년에서 치주질환 원인균의 조성을 알아보고자 전신질환이 없는 어린이 및 청소년 65명을 대상으로 치은연하의 치태를 채취하여 5종의 치주질환 원인균의 출현율과 그 양을 측정하고, 또한 함께 측정한 치태지수 및 치은지수와의 상관관계를 조사하여 다음과 같은 결론을 얻었다. 1. 치태지수의 평균은 1.33이었으며, 치은지수의 평균은 0.97이었다. 치태지수는 나이가 증가할수록 낮아졌다(p<0.05). 2. 치주질환 원인균의 출현율은 P. gingivalis 61.5%, T. forsythia 53.8%, T. denticola 29.2%, A. actinomycetemcomitans 15.4%, F. nucleatum은 100%였다. P. gingivalis, T. denticola는 10세 이후 출현율이 증가하였고 A. actinomycetemcomitans는 8-10세 군에서 출현율이 높았다(p<0.05). 3. 정량적 분석에서 치태지수와 치은지수는 F. nucleatum의 양과 유의한 상관성이 있었으며, 치태지수는 치은지수와 유의한 상관성이 있었다. T. forsythia는 A. actinomycetemcomitans, T. denticola양과 강한 상관성을 보였으며, T. denticola양과 A. actinomycetemcomitans도 강한 상관관계를 가지고 있었다.

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A Simple Emergence Model of Southern Type Garlic Based on Temperature (온도에 따른 난지형 마늘 출현 모형)

  • Moon, K.H.;Choi, K.S.;Son, I.C.;Song, E.Y.;Oh, S.
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.16 no.4
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    • pp.343-348
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    • 2014
  • We developed a simple model to predict emergence time and emergence rate of southern type garlic using the daily mean temperature. Emergence rate of garlic was decreased and emergence time was delayed on higher temperature than optimum temperature of $12.7^{\circ}C$. In the model, firstly daily emergence rate was calculated using a beta function to input daily mean temperature, then the percentage of garlic emergence was calculated using a nonlinear model with accumulated emergence rate. The model was good to describe the experimental data of growth cabinet. Also it can explain well the experimental data using temperature gradient tunnel, designed for verification of model performance. But there are 5 days of deviation between estimated and measured time of garlic emergence on the field experiment. More research is needed to develop an advanced model considering other factors, such as soil moisture.

Analysis of Carcass Quality Grades According to Gender, Backfat Thickness and Carcass Weight in Pigs (비육돈의 성별·등지방두께 및 도체중별 육질등급 분석)

  • Kim, Gye-Woong
    • Journal of Animal Science and Technology
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    • v.54 no.1
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    • pp.29-33
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    • 2012
  • This study was carried out to investigate carcass grade of pigs according to gender, feed intake, backfat thickness, carcass weight, etc. Data from a total of 26,983 heads in 5 farms were collected. The results obtained were as follows; The difference was significantly found in carcass quality grade between barrows and gilts (p<0.001). The meat grade $1^+$ was high in gilts, but meat grade 1 was high in barrows, respectively. The carcass weight of 85.8 kg was lightly shown significantly (p<0.05) in group I taken little quantity of feed consumption. However, that of 89.5 kg was heavily shown in group IV taken plenty of feed consumption. Meat grade 2 was significantly (p<0.001) high ratio in group of backfat thickness with less than 15 mm (67.5%). However, the ratio for the grade 1 was high in group of 15~19 mm. The ratio for the meat grade 2 was highly shown in group of carcass weight with more than 85 kg (54.3%). On the other hand, that of grade 1 was highly shown in group of 78~84 kg. In conclusion, pigs for marketing to enhance high quality have been appropriately raised with the optimum system in consideration of feed intake, carcass weight, backfat thickness, etc.

Observation of Items Fed by Noctiluca Scintillans around Dokdo in Spring (춘계 독도 주변해역 야광충의 먹이생물)

  • KANG, JUNG-HOON
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.25 no.4
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    • pp.160-172
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    • 2020
  • To understand on-site feeding traits of Noctiluca scintillans with unprecedented high abundances around Dokdo in spring, 2014, the composition and abundance of food items in the digestive vacuole of the species were analyzed. Abundances of N. scintillans ranged from 4,328~17,791 inds.m-3 around Dokdo during the study. The incidence of preyed items in the vacuole of N. scintillans ranged by averaged 32% (24~50%) in the surface waters around Dokdo. Diverse preyed items consisted of fecal pellet, protozoans, pollen, diatoms, dinoflagellates, copepods and ichtyoplankton, while fecal pellets were dominantly fed by N. scintillans (43%) and next by protozoans (19%), pollen (18%), diatoms (7%) and dinoflagellates (4%). Fecal pellet, protozoans and pollen were relatively preferred by N. scintillans compared to diatoms, dinoflagellates and ichtyoplankton based on the incidence in the food vacuoles. Present results indicated that high abundance of N. scintillans found around Dokdo was likely associated with non-phytoplankton items rather than previously well-known diatoms as prey during the study.

The Relationships Between Mycoplasmic Pneumonia and Production Traits in Pigs (돼지의 마이코플라즈마성 폐렴과 경제형질간의 상관관계 연구)

  • Yoo, Im-Jong;Oh, Hyung-Gil;Park, Byung-Suk;Lee, Ha-Bok;Lee, Jong-Gwan;Jeon, Byung-Kook;Kim, Na-Rae;Lee, Jun-Heon
    • Journal of Animal Science and Technology
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    • v.50 no.4
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    • pp.437-444
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    • 2008
  • Pneumonia is one of the important diseases in commercial pig. In order to delineate the relationships with pneumonia and productions traits, five different economic traits including carcass weight(CW), backfat thickness(BF), meat quality(MQ), carcass characteristics(CC) and pork belly thickness(PB) were measured in 6,362 commercial pigs. Significant differences were observed in all the traits with pneumonia(P<0.01). Carcass weight and backfat thickness decreased significantly as the symptom of pneumonia was severed (P<0.01). Also, when the pneumonia was severed, the carcass characteristics and meat quality became worse compared with normal unaffected animals(P<0.01). In case of pork belly thickness, the significant decrease was observed in pneumonia infected animals compared with normal animals(P<0.01). Based on this study, the pneumonia infection affects most of the important economic traits in pigs and it is ultimately needed to pay more attention to prevent this disease.

Effects of Scarification and Soaking Treatment on Germination of Sword Bean Seed (작두콩 종자의 종피파상과 침지처리가 발아에 미치는 영향)

  • Hong Soo Doo;Won Jae Ba다;Jeom-Ho Ryu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.3
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    • pp.165-169
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    • 2001
  • Seed coat of sword bean (Canavalia gladiata) is very thick and hard, it is difficult to absorb water during germination and it requires much time that cotyledon come out from seed coat since seminal root appearanced. Therefore this experiment was carried out to increase the germination rate by easing water absorption through mechanical scarification on seed coat. Non-scarification seed did not germinated at 7 days after treatment but scarification seed showed germination rate of 98%. Therefore mechanical scarification of seed coat was increased germination rate. Non-scarification seed absorbed less than 10% water of dry weight at 8 hours after treatment but scarification seed absorbed about 90% water at 7 hours after treatment and more than 150% water at 8 hours and swelled about 1.4-1.5 times in length and width of seed. Germination rate of scarification seed was high under high temperature and it was 96% and 93% at $25^{\circ}C$ and 3$0^{\circ}C$, respectively. The rate of cotyledon emergence on clay loam soil was the highest among bed soils. Soaked seed after mechanical scarification in distilled water was germinated faster than non-soaked seed on early in bed soil. The rate of cotyledon emergence was more than 92% at 9 days after sowing but non-scarification and soaked seed was germinated late and showed very low germination rate as 67%.

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PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN (어린이 치면세균막에서 치주질환원인균과 항생제 내성유전자의 출현율)

  • Kim, Seon-Mi;Choi, Nam-Ki;Cho, Seong-Hoon;Lee, Seok-Woo;Lim, Hoi-Jeong;Lim, Hoi-Soon;Kang, Mi-Sun;Oh, Jong-Suk
    • Journal of the korean academy of Pediatric Dentistry
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    • v.38 no.2
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    • pp.170-178
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    • 2011
  • The purpose of this study was to assess the prevalence of periodontopathic bacteria and resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M), ermF, aacA-aphD, cfxA, $bla_{SHV}$, $bla_{TEM}$, vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia. and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition. the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants. cfxA, $bla_{TEM}$ and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance. $bla_{SHV}$ associated with resistance to ${\beta}$-lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and $bla_{SHV}$. Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).

Effect of Seed-gathering Time and After-ripening on Seed Emergence of Paeonia lactiflora Pall (작약종자(芍藥種子)의 채종시기(採種時期)와 후숙(後熟)이 출아(出芽)에 미치는 영향(影響))

  • Chung, Sang-Hwan;Suh, Dong-Hwan;Kim, Ki-Jae;Lee, Kwang-Seok;Choi, Boo-Sul;Kim, Yong-Han
    • Korean Journal of Medicinal Crop Science
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    • v.1 no.1
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    • pp.10-15
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    • 1993
  • This study was carried out to identify the effect of seed gathering time and after-ripening on Paeonia lactiflora Pall (PL) seed emergence, and to establish optimal seed gathering time and sowing time. We used two local cultivar of PL ; Punggi and Uisung. Seeds were gathered 6 times from 5th July to 25th August in 1992. The seeds of Punggi local cultivar were planted by the planting density of $10\;{\times}\;15cm$ after treatment of 7 ways ; without after-ripening, with after-ripening for 10, 20, 30 days without or with capsule. We investigated the change of 100 grains weight of PL seed by the passage of time and emergence rate after treatment. The seed weight of Punggi was heavier than that of Uisung and the seed weight was maximzed at 25th July both of the two, and then decreased by the passage of time. The seed emergence rate of Punggi gathered at 5th July was showed 7.8% and the seed emergence of Uisung was later 10 days than that of Punggi. The maximum emergence rate of two local cultivars was the highest in gathering the seeds on the 15th of August. In gathering the seeds without and with capsule, the emergence rate of seeds sowed with after-ripening was higher than that of seeds sowed immediately after gathering the seeds without after-ripening when the seeds gathered between 5th July and 5th August. In after-ripening with out and with capsule, the emergence rate of 10 days after-ripening seeds was less than that of 20 and 30 days after-ripening seeds after the seeds gathered on the 5th and 15th of July. Difference of the emergence rate of seeds by after-ripening time did not show when the seeds gathered after 25th July.

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