• The Korean Journal of Food And Nutrition
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• v.23 no.1
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• pp.63-69
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• 2010

With the increase of the use of antibiotics and invasive procedures, infections caused by multidrug-resistant Acinetobacter baumannii(MRAB) are increasing. We screened the antibiotic producing strain B-51 for antibacterial activity against MRAB from the soils and studied the effects of culture medium on the antibiotic production of B-51. The medium conditions for maximum antibiotic productivity of B-51 was 2% glycerol, 0.5% soybean meal, 0.01% $CaCl_2$, 0.01% $MgSO_4{\cdot}7H_2O$ and 0.01% $KH_2PO_4$ at an initial pH of 6.0, at $30^{\circ}C$ for 76 h.

• The Korean Journal of Food And Nutrition
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• v.22 no.1
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• pp.103-109
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• 2009

In order to isolate antibiotic producing microorganisms, several actinomycetes were isolated from soil samples. The aerial hyphae of Y-88 strain were gray in color with tree types. Under the microscopic examination, the Y-88 isolate formed a spiral aerial spore mass with a smooth surface and a rectiflexibilis type of spore chain. Y-88 utilized glucose, fructose, arabinose, and sucrose, but not rhamnose, raffinose, mannitol, or inositol. In addition, Y-88 produced melanin on the tyrosine agar and the strain could utilize L-valine, L-phenylalanine, and L-hydroxyproline. Based on these results and the cultural and physiological characteristics described in the Bergey's Manual, the Actinomycetes, Y-88, was identificated as a Streptomyces species. The optimum medium conditions for this antibiotic producing Streptomyces sp. Y-88 was 1.6% soluble starch, 0.6% glucose, 0.6% beef extract, 0.01% $K_2HPO_4$, 0.01% $MgSO_4$ $7H_2O$, and 0.01% $ZnSO_4$ $7H_2O$ at an initial pH of 4.0, and 25$^{\circ}C$.

• Korean Journal of Food Science and Technology
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• v.25 no.6
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• pp.787-793
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• 1993

A mold producing large amounts of lipid were isolated from leaves, arable soils, rice straw, cow feces, compost heaps and fermented soybeans. Among various sources, the microbes from leaves produced the large amount lipids, which were identified as Aspergillus sydowi SW 4-1. This study was focused mainly on the optimization of cultural conditions for lipid production by A. sydowi SW 4-1 and the characterization of the produced lipids. A. sydowi SW 4-1 was cultivated in a medium containing 27% sucrose for 17 days at $27^{\circ}C$. Biomass was 4.99g/100ml of the cultural medium and lipid content was 41.4% on a dry biomass basis. The major fatty acids were palmitic acid, stearic acid, oleic acid, and linoleic acid.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.53-60
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• 1984

The followings are the results obtained from the investigation of isolation and cultural conditions of a fungus producing antibiotic from humus, spring soil, paddy, field and etc. 1. Among fungi which were capable of antimicrobial sub stance producing at the $45^{\circ}C$, Strain H-3 was selected as test st rain because of its efficiency in antimicrobial substance producing. 2. Strain H-3 was identified as a Aspergullus fumigatus by the mophological properties. 3. The compositions of optimun media for the production of antimicrobial substance were followed;glucose 20g, $NaNO_3$ 3g, $K_2HPO_4$ 1g, $CaCl_2$ 0.5g, $MgSo_4$ 0.5g, $FeSO_4$ 0.005g. 4. Optimum culture conditions were founded to be pH 5.0, $40^{\circ}C$ respectively. 5. The maximum antimicrobial substance producing obtained in this study was showed 14mm of clear zone after 72 hrs incubation under the optimum conditions. 6. AF-c substance was active against Gram-positive, Gram-negative bacteria, and yeast but not mold in its biological properties.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.460-465
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• 2003

A bacterium, named as strain KL34, producing extracellular pectinase was isolated from soil. The mophological cheracteristics of the isolated bacterium were gram-negative, rod-shaped and endospore unformed. Production of pectinase of strain KL34 was induced only by polygalacturonic acid added to the culture media as a sole carbon source. Pectinase activity of KL34 reached a maximum value in the culture conditions of pH 8.5 at $25^{\circ}C$. Optimal medium for pectinase production was determined to the composition of 2% polygalacturonic acid, 0.25% yeast extract, 0.02% $K_2HPO_4$, 0.02% $CaCl_2$, and 0.05% KCl per liter. The pectinase activity in the culture supernatant reached the highest amount of 54 U/ml after 3 days cultivation in the optimal media.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.169-172
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• 2005

본 연구의 목적은 ${\beta}-CD$를 가교화 시켜 육가공 제품의 주원료인 lard의 cholesterol을 효과적으로 제거하기 위한 최적조건 확립이다. 따라서 adipic acid로 가교화한 ${\beta}-CD$를 이용해 lard에서 cholesterol을 제거하고 더 높은 효율을 찾기 위하여 실시되었다. 가교화 ${\beta}-CD$를 이용해 lard에서 cholesterol 제거 실험결과의 최적 조건은 lard : distilled water=1:1, 가교화 ${\beta}-CD$ 5%를 첨가, 교반온도 $40^{\circ}C$, 교반시간 10분, 교반속도 150rpm으로 실험한 결과 cholesterol 제거율이 평균 81.6%이었다. 이 실험의 결과 가교화 ${\beta}-CD$로 lard의 cholesterol 제거 최적조건을 규명하고 이는 콜레스테롤을 저하시킨 육가공 제품의 생산 가능성을 시사하였다.

• Microbiology and Biotechnology Letters
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• v.11 no.1
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• pp.33-38
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• 1983

As flocculent strains are likely to have considerable potential for internal cell recycle, kinetic studies on glucose medium with flocculent Saccharomyces uvarum were carried out in batch and continuous culture. Using a mathematical model, the kinetic parameters at each temperature and pH were estimated in order to establish optimal conditions. It was found that an overall optimum temperature for growth and ethanol production in the range 33-35$^{\circ}C$ was desirable. With regard to the effect of pH, ethanol production by S. uvarum was found to be relatively insensitive to pH value between 4 and 6, with an optimum pH of around 5. At these optimal conditions a maximum ethanol productivity of 12 g/$\ell$/h was determined using semi-batch process together with 5. uvarum.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.05a
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• pp.322-322
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• 2004

최근 기계가공은 제품생산의 리드타임(lead time)의 단축 및 가공시간 단축을 통하여 비용을 절감하고 생산성을 극대화하는데 초점이 맞춰지고 있다. 고속가공(HSM： high-speed machining)은 별도의 연마 공정 없이 그 자체로 고품위, 고정도의 가공물을 생성하여 공정을 축소시키며, 가공시간을 단축시켜 생산성을 향상시킬 수 있다. 이러한 고속가공에 있어서 가공특성은 공작기계의 특성, 공구 및 가공조건뿐만 아니라 제품 형상 등의 다양한 요인에 의해 결정되어진다.(중략)

• Microbiology and Biotechnology Letters
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• v.21 no.1
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• pp.1-5
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• 1993

The strain which produced glutathione was isolated from soil samples. The isolated strain was identified as the genus Candida through its morphological. cultural and physiological characteristics. The highest production of glutathione by Candida sp. was obtained after cultivation with shaking for 36 hours in the culture medium containing fructose 1.0%, yeast extract 4.0%. NaCI 0.04%, thiamine-HCI $5{\mu}g$/ml, and L-cysteine 0.04%. The optimal pH and temperature for the glutathione production were pH 6.0 and $25^{\circ}C$, respectively. The glutathione production by Candida sp. under the optimal culture condition was $92{\mu}g/ml$.

• The Korean Journal of Mycology
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• v.32 no.2
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• pp.138-141
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• 2004

The effect of culture parameters on the production of Colletotrichum gloeosporioides growth inhibitory substance from Bacillus subtilis was investigated. The maximal growth inhibition zone was observed in the medium of pH 7.0. Among the tested carbon sources, glucose showed the largest growth inhibition zone above two fold than other carbon sources. Ammonium sulfate and organic nitrogen sources were effective on the production of growth inhibitory substance. Luria Bertani (LB) medium was the best on the production of antifungal substance from B. subtilis.