The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effect of Culture Parameters on the Production of Growth Inhibitory Substance of Colletotrichum gloeosporioides from Bacillus subtilis

Bacillus subtilis에서 분비되는 Colletotrichum gloeosporioides 생장 저해물질 생산에 미치는 배양조건의 영향

  • Cho, Soo-Jin (Department of Microbiology, Daejeon University) ;
  • Cha, Byeong-Jin (School of Environmental Biology and Horticulture, Chungbuk National University) ;
  • Shin, Kwang-Soo (Department of Microbiology, Daejeon University)
  • 조수진 (대전대학교 이과대학 미생물학과) ;
  • 차병진 (충북대학교 농과대학 환경원예학부) ;
  • 신광수 (대전대학교 이과대학 미생물학과)
  • Published : 2004.12.30
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Abstract

The effect of culture parameters on the production of Colletotrichum gloeosporioides growth inhibitory substance from Bacillus subtilis was investigated. The maximal growth inhibition zone was observed in the medium of pH 7.0. Among the tested carbon sources, glucose showed the largest growth inhibition zone above two fold than other carbon sources. Ammonium sulfate and organic nitrogen sources were effective on the production of growth inhibitory substance. Luria Bertani (LB) medium was the best on the production of antifungal substance from B. subtilis.

고추 탄저병균 Colletotrichum gloeosporioides의 생장을 저해하는 물질을 생산하는 세균인 Bacillus subtilis의 각종 배양조건을 달리하여 최적 생산 조건을 조사하였다. 병원균 생장 저해물질 생산을 위한 배지의 초기 산도는 pH 7.0이 최적이였으며, 탄소원으로는 glucose를 첨가했을 경우 다른 탄소원에 비해 2배 이상 높은 활성을 보였다. 질소원으로는 유기 질소원일 경우 비교적 높은 병원균 생장저해활성을 보였으나, 무기 질소원인 ammonium sulfate에서 가장 높은 활성을 보였다. 여러 가지 배지를 사용하여 조사한 결과, LB(Luria Bertani) 배지의 배양액에서 가장 높은 활성이 나타났다.

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References

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