• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.493-494
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• 2008

• 한국약용작물학회:학술대회논문집
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• 2008.11a
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• pp.350-351
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• 2008

• Korean Journal Plant Pathology
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• v.10 no.2
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• pp.144-147
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• 1994

About 450 phytopathogenic fungal cultures were stored in sterile distilled water ar room temperature by the sterile water storage method, which has been known as a simple, convenient, and long-term storage method of microorganisms. After 12 months, viability and pathogenicity of the stored isolates were tested. Among 205 tested, 175 isolates (84.5%) survived. Of these, Rhizoctonia solani, Botrytis cinerea, Pyricularia oryzae, Phytophthora infestans, and Sclerotinia sclerotiorum showed relatively lower survival rate; 92%, 74.1%, 62.5%, 45.8%, and 30%, respectively. Twenty seven isolates belonging to seven important phytopathogenic fungi were tested for pathogenicity, and all isolates tested maintained pathogenicity until at least 12 months after storage.

• Applied Biological Chemistry
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• v.46 no.2
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• pp.150-153
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• 2003

Methanol extract obtained from Lindera erythocarpa leaves was successively fractionated with n-hexane, ethylacetate, n-butanol, and $H_2O$. From ethylacetate fraction, an active fraction was isolated through repeated silica gel column chromatography and recrystallization, and was identified as a stereoisomer complex of methyllucidone by MS and MMR analyses. The complex showed 85% antifungal activity at 50 {\mu}g/ml$ against the disease wheat leaf rust.

• Microbiology and Biotechnology Letters
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• v.28 no.4
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• pp.202-208
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• 2000

Four bacteria capable of using aniline as a sole source of carbon and energy we4e isolated from river waters. Among them, two strains were identified as Stenotrophomonas maltophilia based on their physiological and biochemical characteristics and 16SrRNA gene sequence and the others as delftia acidovorans. The four strains were able to grow on the mineral salt media containing aniline at concentrations up to 6,000 $\mu\textrm{g}$/ml. Since aniline degradation by S. maltophilia has not been reported so far, the two strains A-s and 51-4 were selected for further studies. They completely utilized aniline in a mineral salt medium containing 300 $\mu\textrm{g}$/ml of aniline as a sole carbon and energy source within 24 hours. Optimum pH and temperature for aniline degradation and cell growth of both strains were 7.0 and $35^{\circ}C$, respectively. In addition, they effectively degraded aniline is waste, underground and river waters containing 300 $\mu\textrm{g}$/ml of aniline. This is the first report of aniline degradation by S. maltophilia strains.

• The Korean Journal of Pesticide Science
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• v.9 no.2
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• pp.132-139
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• 2005

New ${\beta}$-methoxyacrylate derivatives 1-4 having thio-enol side chain were prepared and subjected to in vivo screening for fungicidal activity against phytopathogenic fungi and many of them showed good fungicidal activities against especially rice blast and wheat leaf rust at 100 ppm.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.491-495
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• 1998

A fungal disease of the cultivated mushroom, Ganoderma lucidum, caused by Xylogone sphaerospora was epidemic throughout all cultivation areas in Korea which caused a lot of yield losses in the mushroom production. For controlling the disease, the screening of effective fungicides against the pathogenic fungus were conducted. Thirty seven commercially available fungicides were tested for their inhibitory activities on potato dextrose agar media supplemented with these fungicides at various concentrations. Twenty one fungicides significantly inhibited mycelial growth of the pathogen, Xylogone sphaerospora, but 16 fungicides had no inhibitory effect. Among these 21 fungicides, 17 fungicides also inhibited mycelial growth of Ganoderma lucidum as well, but imazalil, procymidone, triforine, and vinclozolin had no inhibitory effects. However, vinclozolin showed no inhibitory effect on mycelial growth of the mushroom even at the concentration of 50 $\mu\textrm{g}$/ml vinclozolin solution for 2 hours, and then the pathogen was inoculated. After two month-cultivation of the mushroom, over 90% of logs treated with vinclozolin without pathogen inoculation produced fruiting bodies. However, fruiting bodies were not produced form the logs inoculated with the pathogen, but not treated with vinclozolin. Fifty seven percent of logs. which were pre-treated with vinclozolin and then inoculated with the pathogen produced fruiting bodies. Based on the results, vinclozolin is effective for the control of yellow disease of the Ganoderma lucidum caused by Xylogone sphaerospora.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.127-127
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• 2003

벼 도열병 저항성 조절과정을 연구하는 효율적인 방법은 돌연변이를 분리하여 해당유전자를 분석하는 것이다. 이를 위하여 벼에서 제조된 4가지 돌연변이 집단을 이용하여 도열병 저항성 돌연변이를 분리하였다. 실험재료로는 1) fast neturon을 처리하여 제조된 Moroberekan 2,000 라인, 2)T-DNA의 형질전환에 의해서 제조된 화영 1,000 라인, 3)DEB처리에 의해서 제조된 RIL260 3,000라인, 4) gamma ray 처리에 의해서 제조된 상해향혈나 10,000 라인 등이 사용되었다. 병 저항성이 감소된 돌연변이의 분리를 위하여 재료로 사용된 벼 품종과 비친화적 상호작용을 보이는 균주의 포자를 2-3주된 벼 잎에 직접 살포하는 방법을 이용하였다. 균주 접종 7 일 후에 blast lesion을 형성하거나 lesion mimic 표현형을 보이는 돌연변이 등 병저항성이 감소된 라인을 선별하였다. 현재까지 1) Moroberakan 5 라인, 2) 화영 4 라인, 3) RIL260 1 라인 등이 선별되었다. 이와 함께 병저항성이 현저히 증가된 돌연 변이를 선별하기 위하여 친화적인 균주를 사용한 실험에서는 상해향렬나 2 라인이 선별되었다. 선별된 돌연변이는 벼 도열병 저항성 유전자의 분리 및 저항성 조절기작을 연구하는데 효과적으로 사용될 것이다.

• Research in Community and Public Health Nursing
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• v.17 no.3
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• pp.364-375
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• 2006

Purpose: The purpose of the study was to investigate burden and the qualify of life in caregivers who are taking care of home-based rare and incurable disease patients. Methods: The subjects of this study were 300 caregivers of rare and incurable disease patients registered at five health centers in Seoul. A survey was conducted by mail and visit in person during the period from the 25th of March to the 12th of May 2005. Collected data were analyzed through t-test, ANOVA, Pearson's correlation coefficient. Result: The mean burden of caregivers was 3.42, and the mean qualify of life of caregivers was 2.71. Burden and QoL showed significant differences according to caregivers' characteristics such as sender, age, relation to the patient, academic qualification, religion, occupation, monthly household income and perceived health condition. Caregivers' burden was in an inverse correlation with their quality of life. Conclusions: According to the results of this study, rare and incurable disease caregivers' burden and their quality of life were in a significant correlation with each other. In order to improve caregivers' quality of life by reducing their burden, we need to reestablish comprehensive policies for rare and incurable disease management including nursing intervention strategies for caregivers.

• Applied Biological Chemistry
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• v.41 no.6
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• pp.471-476
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• 1998

A synthesis and the screening of new 2-iminothiazolines (IV) of which structures are modified based on a lead compound, thiazoline for development of new agrochemical fungicide were described. Bromination of acetoacetanilides (I) which were prepared by the reaction of diketene with anilines gave the corresponding ${\gamma}-bromoacetoactanilide\;(II)$. Treatment of II with N-phenyl-N'-methyl thiourea (III) afforded IV, structure of which was confirmed by various spectroscopic methods. Antifungal activity of the new IV was tested against six kinds of typical plant diseases (in vivo). The IV with aromatic substituents showed remarkable activity against the Pyricuraria oryzae at 250 ppm in primary screening. The candidates with control value over 90% in primary screening were selected and further tested for second screening at lower concentrations. The IV which has an electron-withdrawing substituent such as halogen, especially fluorine in aryl group showed a higher activity as compared to those with electron-donating group and meta substituent was for optimal position.