Isolation of Antifungal Active Compounds from the Leaves of Lindera erythrocarpa

비목나무(Lindera erythrocarpa) 잎으로부터 항진균성 활성물질의 분리

  • Kwon, Sun-Youl (Department of Plant Resources, Sangju National University) ;
  • Kim, Jin-Ho (Department of Plant Resources, Sangju National University) ;
  • Baek, Nam-Ln (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyunghee University) ;
  • Choi, Gyung-Ja (Bio-Organic Science Division, Korea Research Institute of Chemical Technology) ;
  • Cho, Kwang-Yun (Bio-Organic Science Division, Korea Research Institute of Chemical Technology) ;
  • Lee, Byung-Moo (National Institute of Agricultural Science and Technology, RDA) ;
  • Choi, Yong-Hwa (Department of Plant Resources, Sangju National University)
  • 권순열 (상주대학교 식물자원학과) ;
  • 김진호 (상주대학교 식물자원학과) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 최경자 (한국화학연구원 생물화학연구부) ;
  • 조광연 (한국화학연구원 생물화학연구부) ;
  • 이병무 (농업과학기술원 농약안전성과) ;
  • 최용화 (상주대학교 식물자원학과)
  • 발행 : 2003.05.31

초록

Methanol extract obtained from Lindera erythocarpa leaves was successively fractionated with n-hexane, ethylacetate, n-butanol, and $H_2O$. From ethylacetate fraction, an active fraction was isolated through repeated silica gel column chromatography and recrystallization, and was identified as a stereoisomer complex of methyllucidone by MS and MMR analyses. The complex showed 85% antifungal activity at 50 {\mu}g/ml$ against the disease wheat leaf rust.

비목나무 잎의 메탄올 추출물을 n-hexane, ethylacetate, n-butanol, $H_2O$으로 순차적으로 용매분획하였다. Ethylacetate 분획으로부터 silica gel chromatography를 반복하고 재결정하여 활성물질을 분리 정제하였다. 화합물은 NMR과 MS의 기기분석 결과 methyllucidone의 입체이성질체 혼합물로 구조결정 되었다. 이 혼합물은 밀 붉은녹병에 대하여 $50\;{\mu}g/ml$에서 85%의 방제효과를 나타내었다.

키워드

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