• Title/Summary/Keyword: 총 세균수

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Vertical Variation of Total Bacterial number in Daechung Reservoir (대청호에서 세균의 수직적 분포에 관한 연구)

  • Bing, Sun-Hye;Oh, In-Hye
    • The Journal of Natural Sciences
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    • v.16 no.1
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    • pp.143-154
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    • 2005
  • To define the ecological roles of bacterial community in Daechung Reservoir. Vertical variation of total bacterial numbers were estimated at MAN site for one year with DAPI staining. The bacterial numbers were counted, environmental factors were monitored at the surface, -5m, -10m, -15m and -25 m of MAN sited and tried to explain the vertical bacterial numbers with environmental factors. The bacterial number was $1.6-1.7.0 x10^6$ cells/ml at the surface, $2.3-11.0x10^6$ cells/ml at 5 m depth, $1.2-1.4.0x10^6$ cells/ml at 10 m depth, $1.4-15.0x10^6$ cells/ml at 15 m depth and $1.4-1.3.0x10^6$ cells/ml at 25 m depth. The Mean bacterial number at the surface was more that those at any other depth. The explanation of the vertical total bacterial numbers with environmental factors were suggested.

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The Seasonal Variation of Active Bacterial Abundance in Lake Soyang (소양호에서 활성세균수의 계절적.수직적 변화)

  • 석정현;홍선희;김범철;안태석
    • Korean Journal of Microbiology
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    • v.37 no.1
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    • pp.80-84
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    • 2001
  • Vertical and temporal variations of active and total bacterial abundance were monthly estimated in Lake Soyang from April 1999 to January 2000. The number of total and respiring bacteria was determined directly under microscope by AODC and CTC methods, respectively. The number of total and active bacteria varied from $2.1{\times}10^5 to 3.1{\times}10^6 $,$cells{\cdot}ml^{-1}$ and $1.8{\times}10^4 to 8.0{\times}10^5 $,$cells{\cdot}ml^{-1}$, respectively. The proportions of respiring bacteria to total cell ranged from 3.7 to 44.2% : The proportions was the highest in November 1999 and the lowest in December 2000. The specific activity of${\beta}$-glucosidase divided by total bacteria was$1.6{\times}10^5\;amol{\cdot}cell^{-1}{\cdot}hr^{-1}$in August and$1.4{\times}10^5\;amol{\times}cell^{-1}{\times}hr^{-1}$in September while the specific activity divided by CTC active bacteria was about$3.6{\times}10^5\;amol{\cdot}cell^{-1}{\cdot}hr^{-1},\;24.0{\times}10^5\;amol{cdot}cell^{-1}{cdot}hr^{-1}$. The specific activity of active bacteria in September was 6.7 times higher than that of August. By these data of active bacteria, the new information of aquatic ecosystem was unveiled.

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Dynamics of Total Phosphorus and Attached Bacteria in a Porous Medium Concentrating Nutrients from Low-Nutrient Water (저농도 영양염류를 농축하는 여재에서 총인과 부착세균의 변화)

  • Kim, Ju-Young;Nam, Jong-Hyun;Jung, Da-Woon;Cho, Ahn-Na;Choi, Seung-Ik;Ahn, Tae-Seok
    • Korean Journal of Microbiology
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    • v.45 no.2
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    • pp.133-139
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    • 2009
  • A nutrient-concentrating system was operated to retrieve total phosphorus efficiently from a non-point pollution source. Attached bacteria were expected to play an important role in the system. Phosphorous was concentrated by formation of bacterial biofilms on rubberized coconut fiber media of the system. While concentration of total phosphorus (TP) ranged merely 0.12~0.35 mg/L in the stream water, TP levels in pore water and the media were 0.45~0.86 mg/L and 40.91~242.71 mg/kg, respectively. Total bacterial number (TBN) ranged $0.3\sim2.3\times10^6$ cells/ml in stream water, $0.4\sim4.4\times10^6$ cells/ml in pore water and $0.8\sim1.9\times10^9$ cells/g in media. There was a close correlation between TP and TBN. Based on band profiles in DGGE analyses, bacterial communities in the media were different from that in the stream water. Clostridium spp. were abundant in the stream water while Aquabacterium spp. were dominant species in early stages of biofilm formation in the media. The genera predominant in matured biofilms of the media were Clostridium and Enterococcus.

Heterotrophic Bacterial Community and Alkaline Phosphatase Releasing Bacteria in Lake Soyang (소양호에서의 종속영양세균의 종구성 및 Alkaline phosphatase 분비 세균에 관한 연구)

  • 이동훈;안태석;조규송
    • Korean Journal of Microbiology
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    • v.28 no.3
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    • pp.204-209
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    • 1990
  • The total and heterotrophic bacterial distributions, compositions and alkaline phosphatese actibities were analyzed in Lake Soyang from Sep. 1987 to Aug. 1988. The heterotrophic bacteria was small portion, 0.07-2.63% of total bacterial number which ranged from $3.2{\times}10^{5}$ to $3.2{\times}10^{6}$ cells/${\mu}\ell$. The composition of bacterial community was less diverse in summer and at the fish farm site and Peridinium blooming site. Pseudomonas and Flavo bacterium were the dominant genera in all sites. The highest proportion and activity of alkaline phsophatase was appeared in Flavobacterium, while Pseudomonas was the most predominant group.

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Community Analysis of the Bacteria in Sponges of Lake Baikal by FISH Method (바이칼 호 Sponge에 서식하는 세균 군집 구조의 분석)

  • Seo, Eun-Young;Kim, Mi-Ree;Ahn, Tae-Seok
    • Korean Journal of Microbiology
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    • v.43 no.1
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    • pp.14-18
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    • 2007
  • The bacterial community structures at 2 sponge species belonging to the genus Baikalospongia and Lubomirskia in Lake Baikal were analyzed with fluorescent in situ hybridization (FISH) method. The total bacterial numbers in the genus Baikalospongia ranged from $7.2{\times}10^{7}\;to\;4.2{\times}10^{8}\;cells/ml$, and those in Lubomirskia from $1.2{\times}10^{8}\;to\;1.6{\times}10^{8}\;cells/ml$, while those of lake water were from $2.3{\times}7.7{\times}10^{5}\;cells/ml$. Total bacterial numbers in the sponges were $10^{3}-10^{4}$ times higher than those of lake water. In the genera Baikalospongia and Lubomirskia, the proportions of other unidentified bacterial groups to the Bacteria were 42.0-60.3% and 40.7-51.9%, respectively. These proportions were similar to those in lake water (22.6-46.3%). These results suggest that bacterial compositions in Lake Baikal water and sponges are highly unique.

Storage Characteristics of Irradiated Pacific Oysters, Crassostrea gigas (감마선에 쬔 참굴의 저장성)

  • CHUNG Jong Rak
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.2 no.1
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    • pp.41-46
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    • 1969
  • 산란기 직전의 참굴(Crussostrea gigas)을 통상 방법으로 개각하여 0.3 및 2.0 Mrad의 감마선에 각기 쬔 후 $0^{\circ}C$의 온도하에 저장기간중 번식하는 총 세균수와 pH 변화를 측정하고 부패상을 관찰함으로씨 방사선을 쬔 참굴의 저장성을 조사하였다. 자외선을 쬔 무균해수에 개각하지 않고 정화시킨 굴과 보통 해수에 수용하였던 굴을 비교하여 정화 처리에 의한 세균 제거의 효율을 조사하였으나 4-5일의 장기간 처리에도 불구하고 별다른 세균제거 결과를 볼 수 없었다. 이것은 굴이 보유하고 있는 본래의 총 세균수가 적기 때문이라고 추측된다. 감마선을 쬐지 않은 굴은 저장 15일째에 부패했음이 완연했으나 0.3 Mrad의 감마선을 쬔 굴은 35일후 까지도 시큼할뿐 별로 변질되지 않았다. 2.0 Mrad의 감마선을 쪼인 굴은 관능상의 선도 유지에 있어서 0.3 Mrad로 쬔 굴보다 휠씬 나빴고 off-odor가 심했다. 굴 저장중의 pH 변화로 선도를 판정함은 그 신빙성이 극히 희박하였고 선도의 저하와 함께 pH가 내려간다는 일반적인 인식과는 달리 어류부패의 경우와 같이 저장 초기에는 약간 하강하나 부패의 진행과 함께 상승함이 확인되었다.

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Effect of Salinity on the Bacterial Community in the Sewage Treatment System (하수 처리 과정에서 염분이 세균 군집에 미치는 영향)

  • 서미애;홍선희;김동주;박경미;안태석
    • Korean Journal of Microbiology
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    • v.37 no.2
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    • pp.124-129
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    • 2001
  • For elucidating the effect of salinity to the effect of wastewater treatment, the heterotrophic bacterial numbers, total bacterial numbers, and the bacterial community structure by FISH method were analyzed. The total bacterial numbers were not significantly changed by the salinity. But the heterotrophic bacterial numbers and bacterial community structures were drastically changed by the increase of salinity. In case of 1% salinity, the heterotrophic bacterial numbers and structure were slightly changed comparing to those of contol. In case of 2% and higher salinities, the numbers of heterotrophic bacteria and the proportions of Eubacteria, Proteobacteria $\alpha$-group, $\rho$-group and Cytophaga-Flavobacterium groups were deceasing. By these results, the salinity stress to bacterial community in waste water treatment was unveiled, and for sustaining the waste water treatment system, the salinity should be lower than 1%.

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Distribution and Dynamics of the Total Bacterial Number in the Kyongan Stream and Paltang Reservoir (경안천과 팔당호에서 총세균수의 분포 및 동태)

  • Park, Kyung-Mi;Hwang, Soon-Jin;Cho, Kyung-Je;Shin, Jae-Ki
    • Korean Journal of Ecology and Environment
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    • v.34 no.2 s.94
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    • pp.119-125
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    • 2001
  • Total bacterial density was investigated in the main stream and tributaries of the Kyongan Stream and inlet parts of Paltang Reservoir from September 2000 to February 2001 by acridine orange direct count (AODC) method. Total bacterial number in the Kyongan Stream was mainly under influence of the effluent discharge of sewage wastewater treatment plant (SWTP) located in the upstream or downstream. Decreasing rate with water flowing distance (km) in the main stream is $0.13\;{\time}\;10^6$ cells/ml, and it was estimated to much accumulating quantity on the stream bed during transport to downstream. Average values of total bacterial number in September${\sim}$October, November and December${\sim}$February were range $1.74{\sim}3.10{\time}10^6$, $1.86{\sim}7.30{\time}10^6$ and $4.56{\sim}8.75{\time}10^6$cells/ml, respectively, and were high at low temperature than that of high temperature period. Total bacterial number was more abundant at below $10^{\circ}C$ with $2.1{\sim}3.0$ folds than at above $10^{\circ}C$. Water quality by total bacterial number was classify to eutrophic and the potential of wastewater treated effluent for the microbial contamination assessed to very high. The results of this study indicate that the management of point source, SWTP effluent, is urgent to mitigate bacterial impact of Paltang Reservoir as well as the Kyongan Stream.

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