Browse > Article

Dynamics of Total Phosphorus and Attached Bacteria in a Porous Medium Concentrating Nutrients from Low-Nutrient Water  

Kim, Ju-Young (Department of Environmental Science, Kangwon National University)
Nam, Jong-Hyun (Department of Environmental Science, Kangwon National University)
Jung, Da-Woon (Department of Environmental Science, Kangwon National University)
Cho, Ahn-Na (Department of Environmental Science, Kangwon National University)
Choi, Seung-Ik (Institute of Environmental Research, Kangwon National University)
Ahn, Tae-Seok (Department of Environmental Science, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.45, no.2, 2009 , pp. 133-139 More about this Journal
Abstract
A nutrient-concentrating system was operated to retrieve total phosphorus efficiently from a non-point pollution source. Attached bacteria were expected to play an important role in the system. Phosphorous was concentrated by formation of bacterial biofilms on rubberized coconut fiber media of the system. While concentration of total phosphorus (TP) ranged merely 0.12~0.35 mg/L in the stream water, TP levels in pore water and the media were 0.45~0.86 mg/L and 40.91~242.71 mg/kg, respectively. Total bacterial number (TBN) ranged $0.3\sim2.3\times10^6$ cells/ml in stream water, $0.4\sim4.4\times10^6$ cells/ml in pore water and $0.8\sim1.9\times10^9$ cells/g in media. There was a close correlation between TP and TBN. Based on band profiles in DGGE analyses, bacterial communities in the media were different from that in the stream water. Clostridium spp. were abundant in the stream water while Aquabacterium spp. were dominant species in early stages of biofilm formation in the media. The genera predominant in matured biofilms of the media were Clostridium and Enterococcus.
Keywords
biofilm; phosphorus; DGGE;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 남귀숙, 손형식, 차미선, 조순자, 이광식, 이상준. 2003. 이화학적 수질인자가 부영양화된 마산저수지의 세균분포에 미치는 영향. 한국미생물학회지 39, 95-101   과학기술학회마을
2 환경부. 2008. 생태공학적 방법을 이용한 수역의 부영양화 방지기술 보고서
3 Acinas, S.G., J. Anton, and F. Rodriguez-Valera. 1999. Diversity of free-living and attached bacteria in offshore Western Mediterranean waters as depicted by analysis of genes encoding 16S rRNA. Appl. Environ. Microbiol. 65, 514-522   PUBMED   ScienceOn
4 APHA. American Public Health Association. 2001. Standard method for the examination of water and wastewater
5 Bitton, G. 2002. Encyclopedia of environmental microbiology, pp. 861-871. Wiley. New York, USA
6 Muyzer, G., E.C. de Waal, and A.G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59, 695-700   PUBMED   ScienceOn
7 노상덕, 김장현, 이대근, 김선주, 손병용, 전야근. 2006. 횡성호 유역의 비강우시 및 강우시 오염물질 유출특성. 한국물환경학회지 22, 695-705
8 정부합동. 2000. '98.'00 4대 강 수계 물관리 종합대책
9 홍선희, 김옥선, 전선옥, 유재준, 안태석. 2002. 해빙기 바이칼호에서 부유세균과 aggregates에 부착한 세균의 군집구조. 한국미생물학회지 38, 192-197   과학기술학회마을
10 안태석. 2008. 탕정단지 폐수방류수를 이용한 생태공간 조성에 대한 컨설팅 용역 보고서. 삼성전자주식회사
11 Donlan, R.M. and J.W. Costerton. 2002. Biofilms: Survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 15, 167-193   DOI   ScienceOn
12 이현동, 안재환, 배철호, 김운지. 20001. 강우시 유출부하량을 이용한 팔당상수원 유역의 비점오염원 원단위와 발생량 추정. 한국물환경학회지 17, 313-326
13 안태석. 2005. 한강수계 생태계 복원방법 및 기술표준화 연구. 한강수계관리위원회
14 Karny, G., H. Horn, and T.R. Neu. 2008. Interaction between biofilm development, structure and detachment in rotating annular reactors. Bioprocess Biosyst. Eng. 31, 619-629   DOI   ScienceOn
15 Thormann, K.M., R.M. Saville, S. Shukla, and A.M. Spormann. 2005. Induction of rapid detachment in Shewanella oneidensis MR-1 biofilms. J. Bacteriol. 187, 1014-1021   DOI   ScienceOn
16 안태석, 최승익, 남종현. 2009. 영양염 농축 및 농축수 회수 장치. 특허등록 10-0878368
17 안태석. 2004. 여재를 이용한 자연순환방식의 수질정화 시스템 개발. 강원지역환경기술개발센터
18 Kjelleberg, S. and M. Hermansson. 1984. Starvation induced effects on bacteria surface characteristics. Appl. Environ. Microbiol. 48, 497-503   PUBMED   ScienceOn
19 Lehman, R.M. and S.P. O'Connell. 2002. Comparison of extracellular enzyme activities and community composition of attached and free-living bacteria in porous medium columns. Appl. Environ. Microbiol. 68, 1569-1575   DOI   ScienceOn
20 김용전, 허재규, 남종현, 김인선, 최경숙, 최승익, 안태석. 2007. 파로호에 설치된 인공식물섬 식생기반재의 공극수에서 세균 분포와 체외효소활성도. 한국미생물학회지 43, 40-46   과학기술학회마을
21 Liesack, W., H. Weyland, and E. Stackebrandt. 1991. Potential risks of gene amplification by PCR as determined by 16S rDNA analysis of a mixed-culture of strict barophilic bacteria. Microb. Ecol. 21, 191-198   DOI   ScienceOn
22 Beverideg, T.J., S.A. Makin, J.L. Kadurugamuwa, and Z. Li. 1997. Interactions between biofilms and the environment. FEMS Microbiol. Rev. 20, 291-303   DOI   ScienceOn
23 Delong, E.F., D.G. Franks, and A.L. Alldredge. 1993. Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol. Oceanogr. 38, 924-934   DOI   ScienceOn
24 Mueller, R.F. 1996. Bacterial transport and colonization in low nutrient environments. Water Res. 30, 2681-2690   DOI   ScienceOn
25 Wang, L., J. Lyons, P. Kanehl, R. Bannerman, and E. Emmons. 2000. Watershed urbanization and changes in fish communities in southeastern Wisconsin streams. J. Am. Water Res. 36, 1173-1175   DOI   ScienceOn
26 Schweitzer, B., I. Huber, R. Amann, and W. Ludwig. 2001. $\alpha$-and $\beta$-Proteobacteria control the consumption and release of amino acids on lake snow aggregate. Appl. Environ. Microbiol. 67, 632-645   DOI   ScienceOn
27 Kalmbach, S., W. Manz, J. Weche, and U. Szewzyk. 1999. Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system. Int. J. Syst. Bacteriol. 49, 769-777   DOI   ScienceOn
28 Davies, D.G. and A. McFeters. 1988. Growth and comparative physiology of Klebsiella oxytoca attached to granular carbon particles and in liquid media. Microb. Ecol. 15, 165-175   DOI   ScienceOn
29 Hobbie, J.E., R.J. Daley, and S. Japer. 1977. Use of nucleopore filters for counting bacter by flourescence microscopy. Appl. Environ. Microbiol. 33, 1225-1228   PUBMED   ScienceOn