• Title/Summary/Keyword: 총활성

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Antioxidant Activity of Korean Traditional Soy Sauce (한국 전통 간장의 항산화 활성)

  • Lee, Sangki;Jeong, Yoonhwa;Yim, Seoung Been;Yu, Sungryul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.9
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    • pp.1399-1406
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    • 2015
  • The purpose of this study was to investigate the antioxidant activity of Korean traditional soy sauce and its relationship with physicochemical properties. Seventeen samples of soy sauce prepared by using a Korean traditional method were obtained from six provinces of Korea. The most powerful antioxidant activity of soy sauce was ranging from 8.96 to 63.39% for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, from 0.12 to 2.41 AE mg/mL for ferric reducing antioxidant power (FRAP) value, and from 8.42 to 115.69 TE mg/mL for oxygen radical absorbance capacity (ORAC). Protease activity and total polyphenol contents were highly correlated with antioxidant activities such as DPPH radical scavenging activity and FRAP value (R=0.97). The contents of amino nitrogen were also correlated with DPPH radical scavenging activity (R=0.92), FRAP (R=0.93), and ORAC values (R=0.80). Based on these results, antioxidant activities were highly influenced by protease activity, contents of total polyphenols, and amino nitrogen.

Comparison of Total Polyphenols, Total Flavonoids, and Biological Activities of Black Chokeberry and Blueberry Cultivated in Korea (국내산 Black Chokeberry와 Blueberry의 총 폴리페놀, 총 플라보노이드 함량 및 생리활성 비교)

  • Chung, Hai-Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.9
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    • pp.1349-1356
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    • 2014
  • This study investigated the biological activities of 70% methanol extracts from black chokeberry and blueberry by measuring DPPH, superoxide anion and ABTS radical scavenging activities, metal chelating effect, reducing power, and nitrite scavenging activity. Extraction yields of black chokeberry and blueberry were 62.90% and 67.09%, respectively. Total polyphenol and total flavonoid contents were 117.20 mg/g and 32.50 mg/g for black chokeberry and 42.26 mg/g and 26.39 mg/g for blueberry, respectively. Black chokeberry had higher antioxidant activity than blueberry. DPPH and ABTS radical scavenging activities, nitrite scavenging activity, and metal chelating effect of black chokeberry were 98.29%, 96.68%, 97.64%, and 91.33%, respectively, at a concentration of 5 mg/mL, which were equal to those of ascorbic acid (positive control). These results suggest that black chokeberry has potent biological activities, and in the future, the availability of black chokeberry will increase in the field of value added food products.

Determination of Antioxidant Activity of Edible Calendula Flowers by Hot-air Drying Time (열풍건조 처리시간에 따른 식용꽃 금잔화의 항산화능 검정)

  • Oh, Sang Im;Kim, Seo Young;Lee, Ja Hee;Lee, Ae Kyung
    • FLOWER RESEARCH JOURNAL
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    • v.26 no.4
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    • pp.202-208
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    • 2018
  • This experiment was conducted to determine optimal hot-air drying time for producing antioxidative calendula tea. The edible calendula was dried in hot air at $60^{\circ}C$ for 5, 10, 15 hours and Hunter's color value was measured and extracted with 95% ethanol as a solvent. The contents of total polyphenol, total flavonoid, total carotenoid and DPPH (2,2 Dipheny-1-picrylhydrazyl) radical scavenging activity were investigated. The correlation between the amount of antioxidant and antioxidant activity was analyzed. As the treatment time increased, the Hunter values of L, a, and b tended to decrease, and the L value and b value decreased significantly at 15 hours. The color change was occurred in all treatments compared with the control, and the total color difference (${\Delta}E$) was the largest at 15 hours treatment. The contents of total flavonoids, total carotenoids and DPPH scavenging activity increased in the 5 hours, but decreased in the 15 hours compared to the 10 hours. The content of total polyphenol was not changed with different treatment time. A positive correlation ($p{\leq}0.01$, r = 0.610) between carotenoids and DPPH radical scavenging activity was demonstrated. The DPPH radical scavenging activity was increased at 5 and 10 hours compared to the control and decreased at 15 hours. Therefore, 5 hours or 10 hours of treatment is appropriate and further studies are needed to determine the specific treatment time.

Antioxidant Activity of Ethanol Extract from Different Parts of 5 Types of Late Maturing Citrus (Citrus hybrid 'Shiranuhi', Citrus hybrid 'Setoka', Citrus hybrid 'Blanco', Citrus hybrid 'Kanpei', Citrus 'Kara') (만감류 5종(한라봉, 천혜향, 황금향, 레드향, 카라향) 부위별 에탄올 추출물의 항산화 활성)

  • Ji-Won Seo;Mi-Kyeong Jang;Won-Seob Song
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.66-66
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    • 2023
  • 만감류 5종(한라봉, 천혜향, 황금향, 레드향, 카라향) 부위별 에탄올 추출물의 항산화 활성에 관하여 총 폴리페놀 함량 측정, 총 플라보노이드 함량 측정, DPPH radical 소거 활성 실험을 실시하였다. 만감류 5종(한라봉, 천혜향, 황금향, 레드향, 카라향)의 총 폴리페놀 함량의 경우, 레드향 과피 처리구(93.97mg/g)에서 가장 높았으며, 천혜향을 제외한 모든 만감류 품종 부위들 가운데, 과피 처리구에서 높은 폴리페놀 함량을 나타내었다. 만감류 5종(한라봉, 천혜향, 황금향, 레드향, 카라향)의 총 플라보노이드 함량은 한라봉 전체 처리구(23.65mg/g)에서 가장 높았으며, 모든 처리구에서 30mg/g 함량보다 낮은 결과를 나타내어 총 폴리페놀 함량 대비 낮은 함량을 나타내었다. 이러한 현상은 만감류 5종(한라봉, 천혜향, 황금향, 레드향, 카라향) 가운데 과피 두께의 비율이 영향을 미친 것으로 판단되어진다. 만감류 5종(한라봉, 천혜향, 황금향, 레드향, 카라향)의 DPPH radical 소거 활성의 경우, 한라봉 전체(81.01%)를 시작으로 한라봉 중피(73.13%), 한라봉 외피(63.94%) 처리구에서만 DPPH radical 함량이 EDA 50% 이상 값을 나타내었다. 특히 총 폴리페놀 함량(mg/g)과 총 플라보노이드 함량(mg/g) 모두에서 높은 수치를 기록했던 처리구들 가운데 한라봉 중피(79.75, 23.16), 한라봉 전체(75.51, 23.65) 처리구에서 높은 항산화 활성을 보인 반면, 레드향 과피(93.97, 20.59)의 활성은 49.26%로 DPPH radical EDA 50%에 미치지 못하였다. 이러한 결과는 과피와 과육의 에센셜 오일과 성분의 차이에서 기인된 것으로 추측된다.

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Radical Scavenging Activities and Antioxidant Constituents of Oriental Melon Extract (참외 추출물의 라디칼소거활성과 항산화 성분)

  • Kim, Hye-Suk;Hong, Mi-Jeong;Kang, In-Yeong;Jung, Ji-Youn;Kim, Hye-Kyung;Shin, Yong-Seub;Jun, Ha-Joon;Suh, Jun-Kyu;Kang, Young-Hwa
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.442-447
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    • 2009
  • Extracts from various parts of oriental melon were obtained and antioxidant property and antioxidant constituents including total phenol, total flavonoid, total vitamin C were examined. Free radical scavenging activity was measured by DPPH and ABTS method. Peel part of oriental melon showed the most potent scavenging activities against DPPH and ABTS radicals. The contents of total phenol, total flavonoid in peel were higher than other parts except vitamin C. The amount of vitamin C was the highest in placenta. The relationship between antioxidant activities and antioxidant constituents was determined and showed higher correlation coefficients between antioxidant activities and content of total phenol than other constituents. The above results suggest that phenolic compounds affect antioxidant activity of oriental melon and oriental melon has a good promise as functional food for enhancing health.

Alterations of Glutathione Peroxidase Patterns by Stressor Treatment in Rice Seedling Roots (스트레스 물질에 의한 벼 glutathione peroxidase 활성패턴 변화)

  • Kim, Yoon-Kyoung;Lee, Mi-Young
    • Applied Biological Chemistry
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    • v.48 no.1
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    • pp.53-59
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    • 2005
  • The effect of various stressors such as reductant ascorbic acid, signalling molecules (salicylic acid and methyl jasmonic acid), heavy metals $(NiCl_2,\;and\;MnSO_4)$ and NaCl on the glutathione peroxidase (GPX) activities and isoenzyme expression patterns were investigated in rice seedling roots. Total GPX activity increased according to the increase of ascorbic acid concentration. Prominent enhancement of GPX1 isozyme due to ascorbic acid contributed to the increase of total GPX activity. GPX showed different reactivity toward salicylic acid and methyl jasmonic acid. GPX activity increased at 0.1 mM salicylic acid, and then decreased thereafter. However, GPX increased gradually in a methyl jasmonic acid concentration-dependent manner, and 3 fold increase of GPX activity was found at 1 mM methyl jasmonic acid. Moreover, GPX1 isozyme increased according to the increase of salicylic acid, while GPX1 isozyme decreased according to the increase of methyl jasmonic acid. When metal ions were treated, GPX activity increased considerably according to the increase of $NiCl_2$ concentration, however, GPX activity increased about 2 fold at 0.5 mM $CuSO_4$ and then decreased. Enhancement of GPX1 isozyme contributed to the increase of total GPX activities in $NiCl_2-treated$ and $MnSO_4-treated$ rice seedlings. Total GPX activity increased 1.7 fold in response to 300 mM NaCl. Especially GPX2 isozyme showed gradual increase according to the increase of NaCl concentration.

Studies on Antioxidant Activity, Total Flavonoids and Polyphenols, and Reducing Power in Yakju with Different Ratios of Dandelion Root (민들레 뿌리를 첨가한 약주의 총 플라보노이드, 총 폴리페놀 함량과 전자공여능, 환원력 활성 조사)

  • Lee, Jong-Bok;Park, Hye-Kyong;Lee, Jong-Suk;Kim, Myung-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.21 no.6
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    • pp.882-887
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    • 2011
  • We investigated the physicochemical and antioxidant activity characteristics of Yakju with different ratios (0, 5, 10, and 15%) of dandelion (Taraxacum officinale) root powder in order to find out potential as a functional fermented rice alcohol. Yakju samples were measured to obtain total flavonoid content, total polyphenol content, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, and reducing power. Total flavonoid and polyphenol contents increased with increasing amounts of dandelion root. The DPPH radical scavenging activity increased with the addition of dandelion root. Also, the reducing power was higher in Yakju with dandelion root compared with controls such as gallic acid, butylated hydroxytoluene, and ${\alpha}$-tocopherol. It is concluded that the Yakju fermented with additional dandelion root is functionally superior to the rice alcohol without dandelion root.

Comparative Analysis of Bioactive Compounds and the Sensory Evaluation of Korean Chili (Capsicum annuum L.) Ketchup and American Chili Ketchup (미국 고추케찹 및 국내산 고추케찹의 생리활성 성분 및 관능특성 분석)

  • Yoo, Kyung-Mi
    • The Korean Journal of Food And Nutrition
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    • v.27 no.1
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    • pp.105-111
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    • 2014
  • The purpose of this study is to analyze the Korean chili (Capsicum annuum L.) ketchup and American chili ketchup for their comparative bioactive components and then conduct a sensory evaluation. American chili ketchups are collected from Americans' local favorites, and then the American chili ketchups and Korean chili ketchups are analyzed based on their physiological compositions. Bioactive analysis of the Korean chili ketchups show : total capsaicinoids of $2.59{\pm}1.00mg/100g$, total carotenoids of $33.28{\pm}1.52mg/100g$, total phenol of $61.17{\pm}8.08mg/GAE/100g$, and vitamin C equivalent antioxidant capacity (VCEAC) of $187.04{\pm}3.22mg/100g$. The Korean chili ketchups show higher overall acceptability when being compared to Americans' local favorite chili ketchups. In the sensory evaluation, there are significant differences (p<0.05) in sour, sweet, hot, salty taste, and the overall acceptability in two groups.

The Seasonal Variation of Active Bacterial Abundance in Lake Soyang (소양호에서 활성세균수의 계절적.수직적 변화)

  • 석정현;홍선희;김범철;안태석
    • Korean Journal of Microbiology
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    • v.37 no.1
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    • pp.80-84
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    • 2001
  • Vertical and temporal variations of active and total bacterial abundance were monthly estimated in Lake Soyang from April 1999 to January 2000. The number of total and respiring bacteria was determined directly under microscope by AODC and CTC methods, respectively. The number of total and active bacteria varied from $2.1{\times}10^5 to 3.1{\times}10^6 $,$cells{\cdot}ml^{-1}$ and $1.8{\times}10^4 to 8.0{\times}10^5 $,$cells{\cdot}ml^{-1}$, respectively. The proportions of respiring bacteria to total cell ranged from 3.7 to 44.2% : The proportions was the highest in November 1999 and the lowest in December 2000. The specific activity of${\beta}$-glucosidase divided by total bacteria was$1.6{\times}10^5\;amol{\cdot}cell^{-1}{\cdot}hr^{-1}$in August and$1.4{\times}10^5\;amol{\times}cell^{-1}{\times}hr^{-1}$in September while the specific activity divided by CTC active bacteria was about$3.6{\times}10^5\;amol{\cdot}cell^{-1}{\cdot}hr^{-1},\;24.0{\times}10^5\;amol{cdot}cell^{-1}{cdot}hr^{-1}$. The specific activity of active bacteria in September was 6.7 times higher than that of August. By these data of active bacteria, the new information of aquatic ecosystem was unveiled.

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Antioxidant and Neuronal cell protective effects of Stachys sieboldii Miq. according to drying methods (건조방법에 따른 초석잠(Stachys sieboldii Miq.)의 항산화 활성 및 신경세포 보호효과)

  • Oh, Hee-Kyung
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1320-1326
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    • 2018
  • This study investigated the antioxidant and neuronal cell protective effects of activity of water and 70% ethanol extracts from Stachys sieboldii Miq. according to different drying method(hot air drying and freeze-drying). The total flavonoid and total polyphenol content in water extracts was significantly higher after freeze-drying compared to hot air drying(p<0.05). DPPH and ABTS radical scavenging were increased in a dose-dependent manner. The DPPH and ABTS radical scavenging activity of water extract were significantly higher after free-drying compared to hot air drying(p<0.05). In a cell viability using MTT, the water extract according to hot air drying and freeze-drying of Stachys sieboldii Miq. showed protective effect against H2O2-induced nurotoxicity. The results suggest that the water extracts of Stachys sieboldii Miq. after freeze-drying has antioxidant activities and may be useful for neurodegenerative disorders.