Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidant and Neuronal cell protective effects of Stachys sieboldii Miq. according to drying methods

건조방법에 따른 초석잠(Stachys sieboldii Miq.)의 항산화 활성 및 신경세포 보호효과

  • Oh, Hee-Kyung (Department of Bio-animal care, Jangan University)
  • 오희경 (장안대학교 건강과학부 바이오동물보호과)
  • Received : 2018.12.07
  • Accepted : 2018.12.25
  • Published : 2018.12.31
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Abstract

This study investigated the antioxidant and neuronal cell protective effects of activity of water and 70% ethanol extracts from Stachys sieboldii Miq. according to different drying method(hot air drying and freeze-drying). The total flavonoid and total polyphenol content in water extracts was significantly higher after freeze-drying compared to hot air drying(p<0.05). DPPH and ABTS radical scavenging were increased in a dose-dependent manner. The DPPH and ABTS radical scavenging activity of water extract were significantly higher after free-drying compared to hot air drying(p<0.05). In a cell viability using MTT, the water extract according to hot air drying and freeze-drying of Stachys sieboldii Miq. showed protective effect against H2O2-induced nurotoxicity. The results suggest that the water extracts of Stachys sieboldii Miq. after freeze-drying has antioxidant activities and may be useful for neurodegenerative disorders.

건조방법을 달리한 초석잠(Stachys sieboldii Miq.)을 열수 및 70% 에탄올 용매로 추출하여 제조한 후 추출물 농도에 따른 총 flavonoid 및 총 polyphenol 함량, DPPH radical 소거활성, ABTS radical 소거활성, 산화적 스트레스로부터의 신경세포 보호효과에 미치는 효과를 조사하기 위하여 실시하였다. 초석잠 추출물들의 총 flavonoid와 총 polyphenol 함량은 동결건조 열수 추출물에서 각각 가장 높았다(p<0.05). DPPH 및 ABTS radical 소거활성은 농도의존적으로 증가하였으며, $1,000{\mu}g/mL$ 농도에서는 동결건조 열수추출물에서 유의적으로 가장 높게 나타났으나, 대조군인 비타민 C에 비해 유의적으로 낮았다(p<0.05). 신경세포 보호효과는 열품 및 동결건조 열수추출물에서 농도의존적으로 높게 나타났으며, 대조군인 비타민 C에 비해 낮았다(p<0.05). 본 연구를 통하여 열풍건조에 비해 동결건조 초석잠의 열수추출물이 총 flavonoid와 총 polyphenol 함량이 더 높게 함유되어 있어 항산화 활성 및 산화적 스트레스로부터의 신경세포 보호효과가 우수하게 나타내는 것이 관찰되었다.

Keywords

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Fig. 1. Effects of water extracts from Stachys sieboldii Miq. according to hot air drying methods against H2O2-induced cell death in SH-SY5Y cell system.

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Fig. 2. Effects of water extracts from Stachys sieboldii Miq. according to freezedrying methods against H2O2-induced cell death in SH-SY5Y cell system.

Table 1. Total flavonoid and polyphenol of different solvent extracts from Stachys sieboldii Miq. according to drying methods

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Table 2. DPPH radical scavenging of different solvent extracts from Stachys sieboldii Miq. according to drying methods

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Table 3. ABTS radical scavenging of different solvent extracts from Stachys sieboldii Miq. according to drying methods.

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