• Proceedings of the Korean Society of Precision Engineering Conference
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• 1993.10a
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• pp.607-612
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• 1993

원심분리기는 용액 속에 있는 물질을 크기와 밀도 및 모양의 다름을 이용하여 용액으로부터 분리, 정제 및 순화하는 과학기계이며 처리량의 규모에 따라 공업용과 실험실용으로 분류된다. 공업용은 처리량이 많은 반면 실험실용은 처리량이 적으나 분리효과가 좋은 특징을 갖으며 회전속도에 의해 저속, 고속 및 초고속의 원심분리기로 나뉘어진다. 초고속 원심분리기는 최초(30년대)에 침전계수의 측정을 목적으로 연구개발을 시작하였다. 이의 용도에서 사용되는 초고속원심기를 분석용 초고속원심기라 부르나 이는 50년대 이후에 점차적으로 기술적 발전을 가져와 미소물질 분리를 위한 분리용 초고속원심기로 변천이 되어 생물학분야 이외에 항공기,선박,고속철도,석유탐지,원자력,섬유공업과 반도체등 여러 분야에 이용이 되고 있다.

• The Science & Technology
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• v.28 no.10 s.317
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• pp.18-19
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• 1995

매분 10만을 넘게 회전하는 초고속 모터가 상품화 되고 있어 모터의 마하시대가 열리고 있다. 초고속 모터에 의해 탄생된 초고속 원심분리기는 유전자 산업과 의료분야에서 폭넓게 활용되고 있는데 분당30만회전하는 초고속 모터의 등장도 멀지 않아 실현되어 산업계 전반에 획기적인 변혁이 기대되고 있다.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.6
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• pp.145-152
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• 1998

This paper presents an optimum design of a rotor-bearing spindle system for a ultra centrifuge (80,000 RPM) supported by ball bearings with nonlinear stiffness characteristics. To obtain the nonlinear bearing stiffnesses, a ball bearing is modeled in five degrees of freedom and is analyzed quasi-statically. The dynamic behaviors of the nonlinear rotor-bearing system are analyzed by using a transfer-matrix method iteratively. For optimization. we use the cost function that simultaneously minimizes the weight of a rotor and maximizes the separation margins to yield the critical speeds as far from the operating speed as possible. Augmented Lagrange Multiplier (ALM) method is employed for the nonlinear optimization problem. The result shows that the rotor-bearing spindle system is optimized to obtain 9.5％ weight reduction and 21％ separation margin.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1996.10a
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• pp.319-323
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• 1996

\ulcorner\ulcorner\ulcorner\ulcorner 80,000 rpm \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner(ultra-centrifuge)\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner. \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner(critical speed)\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner(separation margin)\ulcorner \ulcorner\ulcorner, \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner-\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner. \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner \ulcorner\ulcorner\ulcorner, \ulcorner\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner(extra slender shaft)\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner. \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner, \ulcorner\ulcorner 1\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner(bumper ring) \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner(guide bearing)\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner. \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner(finite element method)\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner, \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner\ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner \ulcorner\ulcorner\ulcorner \ulcorner\ulcorner(damping)\ulcorner \ulcorner\ulcorner\ulcorner\ulcorner.

• Journal of the Korean Society for Precision Engineering
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• v.15 no.1
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• pp.130-136
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• 1998

A procedure of stress and strength analysis has been proposed for the centrifuge rotor of composite materials of quasi-isotropic laminates. The goal in this study is to maximize the allowable rotating speed, that is, to minimize maximum strength ratio with the given path length by changing the geometric parameter-outer radius and ply angles in quasi-isotropic laminates. Optimum values of the geometric parameter-outer radius and ply angles are obtained by multilevel optimization. All the geometric dimensions and stresses are normalized such that the result can be extended to a general case. Two dimensional analysis at each cross section with an elliptic tube hole subjected to internal hydrostatic pressures by samples as well as the centrifugal body forces has been performed along the height to calculate the stress distribution with the plane stress assumption, and Tsai-Wu failure criterion is used to calculate the strength ratio. The maximum allowable rotating speed can be increased by changing the radii of the outer surface along the height with the maximum strength ratio under the unit value : The optimal number of ply angles maximizing the allowable rotating speed in quasi-isotropic laminates is found to be the half number of tube hole, and the optimal laminate rotation angle is the half of $[{\pi}/m]$. A $[{\pi}/3]$ laminate, for instance, is stronger than a $[{\pi}/4]$ laminate for the centrifuge rotor of 6 tube hole number even though they have the same stiffness.

• Journal of fish pathology
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• v.17 no.2
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• pp.131-137
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• 2004

In the present study, phosphatidylcholine (PC) hydrolyzing enzyme had been isolated from membrane of flounder liver. PC hydrolyzing enzyme solubilized in 1% Triton X -100 from membrane was partially purified by sequential chromatography on Heparin Sepharose CL-6B and Heparin-5PW columns. The products by membrane-bound hydrolyzing enzyme were identified as phosphatidic acid and choline, but in the presence of primary alcohol, phosphatidylethanol was produced at the expense of phosphatidic acid. These data suggest that membrane-bound enzyme may be a PC-phosphoipase D (PLD) type. The enzyme had pH optimum at below 6.0 and temperature optimum at $37^\circ{C}$. The activity of PC-PLD was dose-dependently increased by $Ca^{2+}$ but not $Mg^{2+}$. The activity of PC-PLD was stimulate by PC, PIP2 and PE.

• Journal of fish pathology
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• v.24 no.3
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• pp.255-268
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• 2011

For detection of noroviruses (NVs), we compared various PCR primer sets based on reverse transcription nested PCR (RT-nested PCR) in the water samples from Dong brook in Busan, South Korea. We designed various new primer sets based on the most conserved sequences of the capsid protein gene that react with diverse NVs found in Korea. Designed primer sets (KG1F/KG1R and KG2F/KG2R, named as PNK) for the respective genogroups of NVs, genogroup I and II (GI and GII), were applied to detect NVs in the water samples from Dong brook concentrated with ultracentrifugation. In the application to the water samples, proportion of GI (76.47%) and GII (70.59%) in water samples of Dong brook in RT-nested PCR with the primer sets of this study. However, no significant differences of the proportion of the positive samples were not found between RT-nested PCRs with reported and newly designed primer sets. From the nucleotide sequencing, GI and GII of NVs present in Dong brook were appeared to be the members of 1/2/4/5/9/10 genotypes, and 3/4/5/11/13 genotypes respectively. Appeared genotype 4 of GII known as an one of main genotype found in patients of many Asian countries warned us to consider the risks of norovirus in aquatic environments in southern part of Korea.