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http://dx.doi.org/10.7847/jfp.2011.24.3.255

Genetic analysis of norovirueses in Busan  

Kim, Kwang-Il (Department of Aquatic Life Medicine, Pukyong National University)
Jin, Ji-Woong (Department of Aquatic Life Medicine, Pukyong National University)
Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.24, no.3, 2011 , pp. 255-268 More about this Journal
Abstract
For detection of noroviruses (NVs), we compared various PCR primer sets based on reverse transcription nested PCR (RT-nested PCR) in the water samples from Dong brook in Busan, South Korea. We designed various new primer sets based on the most conserved sequences of the capsid protein gene that react with diverse NVs found in Korea. Designed primer sets (KG1F/KG1R and KG2F/KG2R, named as PNK) for the respective genogroups of NVs, genogroup I and II (GI and GII), were applied to detect NVs in the water samples from Dong brook concentrated with ultracentrifugation. In the application to the water samples, proportion of GI (76.47%) and GII (70.59%) in water samples of Dong brook in RT-nested PCR with the primer sets of this study. However, no significant differences of the proportion of the positive samples were not found between RT-nested PCRs with reported and newly designed primer sets. From the nucleotide sequencing, GI and GII of NVs present in Dong brook were appeared to be the members of 1/2/4/5/9/10 genotypes, and 3/4/5/11/13 genotypes respectively. Appeared genotype 4 of GII known as an one of main genotype found in patients of many Asian countries warned us to consider the risks of norovirus in aquatic environments in southern part of Korea.
Keywords
Norovirus; Water; RT-nested PCR; Genogroup; Genotype;
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1 Abbaszadegan, M., Huber, M.S., Gerba, C.P. and Pepper, I.L.: Detection of enteroviruses in groundwater with the PCR. Applied and Environmental Microbiology, 59:1318-1324, 1993.
2 Jaykus, L.A., De Leon, R. and Sobsey, M.D.: A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization. Applied and Environmental Microbiology, 62: 2074-2080, 1996.
3 Jiang, X., Graham, D.Y., Wang, K. and Estes, M.K.: Norwalk virus genome cloning and characterization. Science 250:1580-1583, 1990.   DOI   ScienceOn
4 Jiang, X., Wang, M., Wang, K. and Estes, M.K.: Sequence and genomic organization of Norwalk virus. Virology 195:51-61, 1993.   DOI   ScienceOn
5 Kageyama, T., Kojima, S., Shinohara, M., Uchida, K., Fukushi, S., Hoshino, F.B., Takeda, N. and Katayama, K.: Broadly reactive and highly sensitive assay for Norwalk-like viruses Based on Real-time quantitative Reverse Transcription-PCR. Journal of Clinical Microbiology, 41:1548-1557, 2003.   DOI   ScienceOn
6 Kageyama, T., Shinohara, M., Uchida, K., Fukushi, S., Hoshino, F.B., Kojima, S., Takai R., Oka, T., Takeda, N. and Katayama, K.: Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to Norovirus in Japan. Journal of Clinical Microbiology, 42:2988-2995, 2004.   DOI   ScienceOn
7 Kapikian, A.Z., Wyatt, R.G., Dolin, R., Thornhill, T.S., Kalica, A.R. and Chanock, R.M.: Visualization by immune electron microscopy of a 27 nm particle associated with acute infectious nonbacterial gastroenteritis. Journal of Virology, 10:1075-1081, 1972.
8 Katayama, H., Simassaki, A., and Ohagaki, S.: Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater. Applied and Environmental Microbiology, 68:1033-1039, 2002.   DOI   ScienceOn
9 Katayama, K., Shirato-Horikoshi, H., Kojima, S., Kageyama, T., Oka, T., Hoshino, F.B., Fukushi, S., Shinohara, M., Uchida, K., Suzuki, Y., Gojobori, T. and Takeda, N.: Phylogenetic analysis of the complete genome of 18 Norwalk-like viruses. Virology 299:225-239, 2002.   DOI   ScienceOn
10 Kim, S.H., Cheon, D.S., Kim, J.H., Lee, D.H., Jheong, W.H., Heo, Y.J., Chung, H.M., Jee, Y. and Lee, J.S.: Outbreaks of gastroenteritis that occurred during school excursions in Korea were associated with several waterborne strains of norovirus. Journal of Clinical Microbiology, 43(9):4836-4839, 2005.   DOI   ScienceOn
11 Kojima, S., Kageyama, T., Fukushi, S., Hoshino, F,B., Shinohara, M., Uchida, K., Natori, K., Takeda, N. and Katayama, K.: Genogroup-specific PCR primers for detection of Norwalk-like viruses. Journal of virological methods, 100:107-114, 2002.   DOI   ScienceOn
12 Belliot, G., Sosnovtsev, S.V., Mitra, T., Hammer, C., Garfield, M. and Green, K.Y.: In vitro proteolytic processing of the MD145 norovirus ORF1 nonstructural polyprotein yields stable precursors and products similar to those detected in Calicivirus-infected cells. Journal of Virology, 77:10957-10974, 2003.   DOI   ScienceOn
13 Anderson, A.D., Garrett, V.D., Sobel, J., Monroe, S.S., Fankhauser, R.L., Schwab K.J., Bresee, J.S., Mead, P.S., Higgins, C., Campana, J. and Glass, R.I.: Outbreak Investigation Team. Multistate Outbreak of Norwalk-like Virus Gastroenteritis Associated with a Common Caterer. American journal of epidemiology, 154:1013-1019, 2001.   DOI   ScienceOn
14 Ando, T., Noel, J.S. and Fankhauser, R.L.: Genetic classification of "Norwalk-like viruses". Journal of Infectious Diseases, 181 (Suppl. 2): S336-S348. 2000.   DOI   ScienceOn
15 Atmar, R.L., Neill, F.H., Romalde, J.L., Le Guyader, F., Woodley, C.M., Metcalf, T.G. and Estes, M.K.: Detection of Norwalk virus and hepatitis A viruses in shellfish tissues with the PCR. Applied and Environmental Microbiology, 61: 3014-30189, 2005
16 Bertolotti-Ciarlet, A., Crawford, S.E., Hutson, A.M. and Estes, M.K.: The 3' end of Norwalk virus mRNA contains determinants that regulate the expression and stability of the capsid protein VP1: a novel function for the VP2 protein. Journal of Virology, 77:11603-11615, 2003.   DOI   ScienceOn
17 Chakravarty, S., Hutson, A.M., Estes, M.K. and Prasad B.V.V.: Evolutionary trace residues in Noroviruses: importance in receptor binding, antigenicity, virion assembly, and strain diversity. Journal of Virology, 79:554-568, 2005   DOI   ScienceOn
18 Dolin, R., Blacklow, N.R., DuPont, H., Buscho, R.F., Wyatt, R.G., Kasel, J.A., Hornick, R. and Chanock, R.M.: Biological properties of Norwalk agent of acute infectious nonbacterial gastroenteritis. Proceedings of the Society for Experimental Biology and Medicine, 140(2):578-583, 1972.
19 Gong, Y.W., Oh, B.Y., Kim, H.Y., Lee, M.Y., Kim, Y.H., Go, J.M. Lee, J.M., Jeong, H.S. and Cheon, D.S.: Molecular Epidemiology Investigation of Norovirus infections in Incheon City, Korea, from 2005 to 2007. Journal of Bacteriology and Virology, 38:249-257, 2008.   DOI   ScienceOn
20 Ho, E.C., Cheng, P.K., Lau, A.W. and Lim, W.W.: Atypical norovius epidemic in Hong Knog during summer of 2006 caused by a new genogroup II/4 variant. Journal of Clinical Microbiology, 45:2205-2211, 2007.   DOI   ScienceOn
21 Nishida, T., Nishio, O., Kato, M., Chuma, T., Kato, H., Iwata, H. and Kimura, H.: Genotyping and quantitation of noroviruses in oysters from two distinct sea areas in Japan. Microbiology and Immunology, 51(2):177-184, 2007.   DOI
22 Lee, C. and Kim, S.J.: The genetic diversity of human noroviruses detected in river water in Korea. Water research, 42(17):4477-4484, 2008   DOI   ScienceOn
23 Mullendore, J.L., Sobey, M.D. and Shieh, Y.C.: Improved method for the recovery of hepatitis A virus from oyster. Journal of Virology Methods 94:25-35, 2001.   DOI   ScienceOn
24 Lewis, G.D. and Meticalf, T.G.: Polyethylene Glycol Precipitation for Recovery of Pathogenic Viruses, Including Hepatitis A Virus and Human Rotavirus, From Oyster, water and Sediment Samples. Applied and Environmental Microbiology, 54:1983-1988, 1988.
25 Richards, G.P., Watson, M.A., Fankhauser, R.L. and Monroe, S.S.: Genogroup I and II Norovirues Detected in Stool Samples by Real-Time Reverse Transcription-PCR Using Highly Degenerate Universal Primer. Applied and Environmental Microbiology, 70:7179-7184, 2004.   DOI   ScienceOn
26 Okada, M., Ogawa, T., Kaiho, I. and Shinozaki, K.: Genetic analysis of norovirueses in Chiba Prefecture, Japan, between 1999 and 2004. Journal of Clinical Microbiology, 43:4391-4401, 2005.   DOI   ScienceOn
27 Park, Y.B. and Ko, G.P.: Development of immunomagnetic separation and receptor-mediated methods for noroviruses. ASM meeting, 2007.
28 Phan, T.G., Khamrin, P., Akiyama, M., Yagyu, F., Okitsu, S., Maneekarn, N., Nishio, O. and Ushijima, H.: Detection and genetic characterization of norovirus in oysters from China and Japan. Clinical Laboratory, 53:405-412, 2007.
29 Center for Disease Control and Prevention. Norovirus activity-United States. Morbidiy and Mortality Weekly Report, 52:41-45, 2002.