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Mass Production of Gain-of-Function Mutants of Hairy Roots in Catharanthus roseus (일일초에서의 기능획득 돌연변이 모상근의 대량생산)

  • Ko, Suk-Min;Chung, Hwa-Jee;Lee, Hyo-Yeon
    • Korean Journal of Plant Resources
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    • v.24 no.5
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    • pp.514-520
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    • 2011
  • This study describes conditions for the mass production of mutant hairy root lines by co-cultivation with A. rhizogenes harboring the activation tagging vector pHC7. Various sources of explants were subjected to genetic transformation with A. rhizogenes to determine optimum conditions and cultivar for the highest frequency of hairy root formation on explants. Hairy root formation also were investigated in transformed hairy roots grown in various culture media. Finally, a total of approximately 2,500 lines of hairy root mutants were produced in this study. A managing system for metabolomics in hairy root lines also were established. These hairy root lines will be useful to determine functions of genes relating biosynthesis pathway of secondary metabolites.

Non-Local Means-based Gradual Super-Resolution via Linear Mappings (비국소적 평균법 기반 점진적 선형 매핑 초해상화 기법)

  • Choi, Jae-Seok;Kim, Munchurl
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2015.07a
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    • pp.75-77
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    • 2015
  • 디스플레이 해상도가 지속적으로 고해상화가 되면서, 기존 저해상도 영상을 고해상도 디스플레이 크기에 맞춰 해상도를 키우는 기법인 초해상화(super-resolution, SR) 기법에 많은 관심이 쏟아지고 있으며 이에 대한 많은 초해상화 논문들이 게재되었다. 이 중 현재 최상 품질의 고해상도 영상을 복원하는 한 초해상화 기법은, 입력 받은 저해상도 영상을 자가 예제(self-examples)로 사용하여 선형 매핑(linear mapping)을 통해 점진적으로 여러 레벨(level)를 거쳐 조금씩 키우는 방법이다. 이때 각 레벨마다 기존 저해상도 영상 크기로 반복적으로 줄여 오차를 줄이는 역투영법(back-projection)을 사용하는데, 이 방법은 처리된 영상에 시각적 품질을 낮추는 링 아티팩트(ringing artifacts)를 생산하며, 이는 매 레벨마다 계속 누적이 되어 고해상도 결과 이미지 품질에 악영향을 미치는 단점이 있다. 이를 보완하기 위해, 본 논문에서는 저해상도 정지 영상을 고해상도 정지 영상으로 점진적으로 키울 때 일반적인 역투영법 대신 비국소적 평균법(non-local means, NLM) 기반 역투영법을 사용하는 초해상화 기법을 제안한다. 제안하는 기법은 매 레벨마다 생기는 링 아티팩트를 효과적으로 제거하여 높은 시각적 품질의 고해상도 영상을 복원할 수 있게 한다. 실험을 통해 제안된 초해상화 기법을 사용 시 기존 초해상화 기법보다 향상된 고품질 고해상도 영상 복원이 가능한 것을 확인하였다.

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Super-Resolution Using NLSA Mechanism (비지역 희소 어텐션 메커니즘을 활용한 초해상화)

  • Kim, Sowon;Park, Hanhoon
    • Journal of the Institute of Convergence Signal Processing
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    • v.23 no.1
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    • pp.8-14
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    • 2022
  • With the development of deep learning, super-resolution (SR) methods have tried to use deep learning mechanism, instead of using simple interpolation. SR methods using deep learning is generally based on convolutional neural networks (CNN), but recently, SR researches using attention mechanism have been actively conducted. In this paper, we propose an approach of improving SR performance using one of the attention mechanisms, non-local sparse attention (NLSA). Through experiments, we confirmed that the performance of the existing SR models, IMDN, CARN, and OISR-LF-s can be improved by using NLSA.

A Study on the Introduction of Bus Priority Signal using Deep Learning in BRT Section (BRT 구간 딥 러닝을 활용한 버스우선 신호도입 방안에 관한 연구)

  • Lim, Chang-Sik;Choi, Yang-Won
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.40 no.1
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    • pp.59-67
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    • 2020
  • In this study, a suitable algorithm for each BRT stop type is presented through the network construction and algorithm design effect analysis through the LISA, a traffic signal program, for the BRT stop type in the BRT Design Guidelines, Ministry of Land, Transport and Maritime Affairs, 2010.6. It was. The phase insert technique is the most effective method for the stop before passing the intersection, the early green technique for the stop after the intersection, and the extend green technique for the mid-block type stop. The extension green technique is used only because it consists of BRT vehicles, general vehicles and pedestrians. Analyzed. After passing through the intersection, the stop was analyzed as 56.4 seconds for the total crossing time and 29.8 seconds for the delay time. In the mid-block type stop, the total travel time of the intersection was 40.5 seconds, the delay time was 9.6 seconds, the average travel time of up and down BRT was 70.2 seconds, the delay time was 14.0 seconds, and the number of passages was 29.

Effect of Angelica keiskei Koidzumi Extract on Alcohol-Induced Hepatotoxicity In Vitro and In Vivo (In Vitro 및 In Vivo 알코올 유도 간 손상에 대한 신선초 추출물의 효과)

  • Lee, Jeong Yoon;An, Yeon Ju;Kim, Ji Won;Choi, Hyo-Kyoung;Lee, Yoo-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.10
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    • pp.1391-1397
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    • 2016
  • We investigated the hepatoprotective effects of Angelica keiskei Koidzumi extract (AK) in HepG2-overexpressing cytochrome P4502E1 (CYP2E1) and C57BL/6J mice. In HepG2 cells expressing CYP2E1, cell viability and catalase activity in the ethanol-AK co-treated group significantly increased compared to those in the ethanol-treated group. In the in vivo study with C57BL/6J mice, the AK-supplemented group with ethanol liquid diet showed significantly reduced hepatic markers, including serum aspartate aminotransferase, alanine aminotransferase, and ${\gamma}$-glutamyl transferase, compared to the ethanol group without AK supplementation. AK supplementation (20 mg/kg BW/d) also significantly attenuated reactive oxygen species generation and malondialdehyde level. Notably, a low dose of AK supplementation (20 mg/kg BW/d) suppressed expression of hepatic CYP2E1 and inhibited CYP2E1 enzyme activity. These data indicate that a low dose of AK supplementation could restrain alcohol-induced hepatic damage mediated by CYP2E1.

A Study on the Growth Models of Sedum takevimense as Affected by Difference of Soil Mixture Ratio in the Green Roof System (토양조성에 따른 옥상녹화용 섬기린초 생장모형 연구)

  • Kang, Tai-Ho;Li, Hong;Zhao, Hong-Xia
    • Journal of the Korean Institute of Landscape Architecture
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    • v.39 no.6
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    • pp.110-117
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    • 2011
  • In order to study the growth models between the growth of Sedum takevimense and growth rate in soil with three types of mix ratios, this experiment was carried out on April 3rd, 2011. A nonlinearity regression analysis was performed using the Logistic and Gompertz models by SPSS. According to the study of growth models of Sedum takevimense, the process of growth and management methods after over-wintering were explicitly determined. According to the measured values, the growth in the soil of $P_1P_2V_1$ and $P_2P_1V_1$ was better than that of $P_1$. Particularly, the average length of Sedum takevimense in the soil of $P_1P_2V_1$ was about twice as great as that in the $P_1$. The fitness test of the two growth models was: The predicted value and measured value were separately compared and analysed, the average fitting precision $R^2$ of the Logistic models was 0.995, but the average $R^2$ of the Gompertz models was below 0.978, which showed that the Logistic models were better than the Gompertz models. The growth models also showed that the growth time of Sedum takevimense was divided into three: rapid, most rapid and slow. When managed in the rapid and the most rapid time, it will grow better.

Effect of Modified Ultrafiltration on Pediatric Open Heart Surgery (소아 개심술시 변형 초여과법(Modified Ultrafiltration)의 효과)

  • 윤경찬;이광숙;유영선;박창권;최세영;최대융
    • Journal of Chest Surgery
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    • v.31 no.1
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    • pp.1-6
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    • 1998
  • Total body water is increased after cardiopulmonary bypass resulting in tissue edema and organ dysfunction. Ultrafiltration has been used to reduce this accumulation of water. We have carried out a prospective randomized study in 17 children undergoing open heart surgery, comparing modified ultrafiltration(MUF) with nonfiltered controls. MUF was carried out for about 10 minutes after completion of cardiopulmonary bypass to a hematocrit 36∼42%. Blood loss, blood transfused, hemodynamics, and laboratory data were recorded for 24 hours postoperatively. The results were analyzed using Mann-Whitney U test, comparing controls(n=7) to ultrafiltered(n=10). There was no death in each group. The mean filtrate volume(ml/kg) was 42(30∼68). Blood loss(ml/kg/24hr) was 14.5 mean(4.0∼26.6) in controls versus 12.1 mean(6.0∼21.5) in MUF(P>0.05) ; blood transfused(ml/kg/24hr) was 9.4 mean (6.0∼36.3) in controls versus 3.4 mean(0∼11.4) in MUF(P<0.05). There was rise in arterial blood pressure during MUF. Percent rise of systolic blood pressure was 4.2(0∼11.7) in controls versus 19.8(7.0∼36.9) in MUF(P=0.001). Percent rise of diastolic blood pressure was 10.0(1.6∼20.8) in controls versus 30.6(5.8∼73.3) in MUF(P<0.05). Platelet count, fibrinogen, and oncotic pressure rose after MUF. No complications directly attributable to the ultrafiltration were observed. Conclusively, MUF is safe, effective means of removing body water and beneficial to hemodynamics.

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The Effectiveness of Metacognitive Instruction Model on the Changes of Molecular Concepts (초인지 수업모형이 초등학생들의 분자개념 변화에 미치는 효과)

  • 신미경;고영신;최영재
    • Journal of Korean Elementary Science Education
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    • v.18 no.2
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    • pp.65-77
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    • 1999
  • The purpose of this study was to find out the effectiveness of metacognitive instruction model on the changes of science concepts, when it was applied to 6th grade students. To do this, students were tested with the achievement of molecules and molecular motion concepts and metacognitive self-regulation test as a pretest Based upon metacognitive instruction model and student's conception, instruction program were developed. This metacognitive strategy Program was applied to the experimental group and expository teaching was applied to the comparison group (followed the order and method in authorized science textbook and teachers handbook). When planned lessons were finished, students were given a post-test to find conceptual change. After six months students were given a test again to find retention effect. There was a significant difference in conceptual change and retention between comparison group and experimental group by treatment at p< .05 level, The difference between comparison group and experimental group was especially significant, when the situation of test item wasn't similar to that of the textbook Metacognitive instruction model was more effective to high group than low group in metacognitive self-regulation level on conceptual change and retention. So the metacognitive strategy Played an important role in conceptual change and retention. And we can recognize that the students who take part in the metacognitive lesson can apply the corrected concept to the other concrete situation because they can understand new concept accurately by metacognitive strategies. And we can guess that high group in metacognitive self-regulation level can team metacognitive strategy easily but relatively low group student have some trouble in learning new strategy.

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Mountain-cultivated Ginseng Ripened into Persimmon Vinegar Ingestion on Fat Storage and Metabolic Protein Expression in Diet-controlled Rats (산양삼 혼입 숙성 감식초 섭취에 의한 식이 제한 흰쥐의 지방 저장 및 에너지 대사 단백질 발현)

  • Lee, In-Ho;Kim, Pan-Ki;Ryu, Sungpil
    • Journal of Korean Society of Forest Science
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    • v.104 no.1
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    • pp.67-75
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    • 2015
  • This research is to investigate the four years growth mountain-cultivated ginseng ripened twenty-two weeks into four years fermented persimmon vinegar (tentatively: Sansamcho) ingestion on obese-related factors during dietary control. The Sansamcho was ingested orally, two times a day, after every meal for six weeks to the male rats. Groups were divided into the control (CON), the restricted diet (RD), and the weight cycling (WC). And, each groups has its own sub-groups as the -control (-CON), 2.5 times diluted Sansamcho ingestion (-MPV2.5), and 5.0 times diluted Sansamcho ingestion (-MPV5.0) groups, respectively. The number of rat was consisted of seven in each group. After six weeks rearing periods was done, abdominal fats (retroperitoneal fat, mesentery fat, and epididymal fat) and energy metabolic-related protein (AMPK: AMP-activated protein kinase; PPAR-${\alpha}$: peroxisome proliferator-activated receptor-${\alpha}$; and CPT-1: carnitine palmitoyltransferase-1) were weighed and analyzed. Amount of stored fat was significantly or tended to decrease by Sansamcho ingestion. In addition, sum of fats increasing were suppressed by the material. On the contrary, energy metabolism-related protein expression was significantly increased or tended to increase by Sansamcho ingestion. This results suggested that increased energy metabolism using Sansamcho was restrained effectively visceral fat store by high-fat diet and/or dietary control. In other words, it has a good function to suppress weight cycling which is the most insoluble problem. Therefore, the fusion material, Sansamcho, may expect to utilize as the obese-suppression-food.

The Protective Effect of Zizania latifolia Extract against t-BHP-induced Oxidative Stress in HepG2 Cells (고장초 추출물의 t-BHP로 산화적 손상이 유도된 HepG2 세포 보호 효과)

  • Park, Se-Ho;Lee, Jae-Yeul;Yang, Seun-Ah;Bang, Daesuk;Jhee, Kwang-Hwan
    • Journal of Life Science
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    • v.31 no.3
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    • pp.338-345
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    • 2021
  • Zizania latifolia has long been used as a tea for both edible and medicinal purposes. However, research into the use of Z. latifolia as a high value-added edible material is lacking. In a previous study, we confirmed that tricin is the major component in Z. latifolia. In this study, we investigated the protective effect of a Z. latifolia extract (ZLE). Toxicity tests of ZLE or tricin on HepG2 cells revealed no toxicity due to ZLE or tricin at all concentrations used. The reduction in cell viability by tert-butyl hydroperoxide (t-BHP) was suppressed by treatment with ZLE or tricin. In addition, ZLE or tricin effectively inhibited the production of reactive oxygen species (generation of hydrogen peroxide, alkoxy free radicals, and peroxyl free radicals by t-BHP) and oxidative damage. ZLE or tricin treatments also increased the protein expression of superoxide dismutase 1 (SOD1), catalase, heme oxygenase-1 (HO-1), and nuclear factor erythroid-related factor 2 (Nrf2), which are known as antioxidant enzymes, suggesting that the protective effect of ZLE is related to activation of tricin. Taken together, the results indicate that Z. latifolia can be developed as a functional food material for improving liver function.