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http://dx.doi.org/10.7732/kjpr.2011.24.5.514

Mass Production of Gain-of-Function Mutants of Hairy Roots in Catharanthus roseus  

Ko, Suk-Min (Subtropical Horticulture Research Institute, Jeju National University)
Chung, Hwa-Jee (Gendocs Inc.)
Lee, Hyo-Yeon (Subtropical Horticulture Research Institute, Jeju National University)
Publication Information
Korean Journal of Plant Resources / v.24, no.5, 2011 , pp. 514-520 More about this Journal
Abstract
This study describes conditions for the mass production of mutant hairy root lines by co-cultivation with A. rhizogenes harboring the activation tagging vector pHC7. Various sources of explants were subjected to genetic transformation with A. rhizogenes to determine optimum conditions and cultivar for the highest frequency of hairy root formation on explants. Hairy root formation also were investigated in transformed hairy roots grown in various culture media. Finally, a total of approximately 2,500 lines of hairy root mutants were produced in this study. A managing system for metabolomics in hairy root lines also were established. These hairy root lines will be useful to determine functions of genes relating biosynthesis pathway of secondary metabolites.
Keywords
Catharanthus roseus; Hairy root; Secondary metabolite; Agrobacterium rhizogenes;
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