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Cell Biological Studies of the Effect of Aromatic Amino Acids on Early Development of Chick Embryo (방향족 아미노산이 초기계배에 미치는 영향에 관한 세포생물학적 연구)

  • 최임순;주충노;최춘근;김재원
    • The Korean Journal of Zoology
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    • v.28 no.4
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    • pp.257-278
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    • 1985
  • The effect of aromatic amino acids such as phenylalaine, tryptophan and tyrosine on somitogenesis at the early stage of chick embryo has been investigated morphologically using light and electron microscopy. Micrographs of aromatic amino acid injected chick embryo showed that an incomplete somite segmentation occurred and some decremental effect on the nervous system were observed. Somites were poorly developed and their size were variable. Electron micrograph of somatic cells from aromatic amino acid injected chick embryo showed that chromatins were coagulated, some of mitochondria were damaged, and nucleus were transformed considerably in some cases. The protein and nucleic acid levels and some enzyme activities of 15-day chick embryo which received the injection of 1mg of aromatic amino acid in 0.05 ml of saline 24 hours after the incubation were analyzed. Protein, DNA and RNA levels of the test group were not lowered significantly but the activities of enzymes for basic metabolism, such as lactate dehydrogenase, succinate dehydrogenase, malate dehydrogenase and glucose 6-phosphate dehydrogenase were considerably lowered as compared with those of control. From the present expeerimental results, it was tentatively suggested that the administration of amino acid might slow down the yolk granule degradation probably by feed back mechanism resulting in the disturbance of amino acid balance in the cell, which might give rise to impair normal metabolic pattern leading to abnormal somitogenesis to chick embryo at very early stage of development.

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The Early-Stage Changes of Water Qualities after the Saemangeum Sea-dike Construction (새만금 방조제 체절 이후 초기의 수질변화에 관한 연구)

  • Yang, Jae-Sam;Jeong, Yong-Hoon;Ji, Kwang-Hee;Kim, Hyun-Soo;Choi, Joeng-Hoon;Kim, Won-Jang
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.11 no.4
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    • pp.199-213
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    • 2008
  • Saemangeum salt-water Lake has been created by the completion of the sea-dike in April 2006. To monitor the water qualities of the lake during the sea-dike construction, salinity, SS, nutrients(DIN, DIP, DISi), and chlorophyll-$\alpha$ was analyzed for the surface water from 1999 to 2007. Due to the dike construction, weaker tidal current and lesser resuspension of bottom sediment resulted in the marked decrease of the concentrations of SS in the lake water. Consequently the clearer lake water has provided better condition for primary production with deeper penetration of sunlight into the water column and sufficient nutrient content in the water. Finally the chlorophyll-$\alpha$ content became approximately double in the concentration after the dike construction. Highly stimulated algal production with the marked decrease of the concentrations of SS was decreased the concentration of DIP in the surface water. On the other hand the concentration of DIN and DISi in surface water was increased after dike construction due to the expansion of the freshwater and the supply from bottom layer. As a result, the lake revealed an extremely high NIP ratio and a DIP-limited ecosystem. The lake has been transformed from a typical coastal ecosystem to a brackish one. Since the dike completion, the lake has shown a similar change pattern to the Geum River estuary. Due to the salt-wedge intrusion of seawater, it is highly probable to expect the formation of low-oxygen zone at the bottom layer near the river-mouth area of the lake during the summer. Therefore we need a continuous sentinel monitoring of bottom water qualities in the near future.

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Simple and Multigrid Models for Hydraulic Study on Coffering a Tide Embankment (방조제 체절 수리 검토를 위한 단순 모형과 다중격자 모형)

  • 이정렬;고진석;이태환
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.13 no.4
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    • pp.309-318
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    • 2001
  • A zero-dimensional simple model is presented to predict the currents in the opening channel of tide embankment and the sea-level response of the interior basin to ocean sea level. In general, a tidal embayment has wide tidal flats so that the effect of tidal flats has been taken into account by formulating the volute variation vs. depth. The model has been verified through the comparison with the 2-dimensional depth-integrated model which can resolve the small area by using the multigrid method. As the results applied to the storage of dredged material of Incheon North Harbour, the results indicate that both models adequately describe the sea-level and current fluctuations in the storage.

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Analysis of Non-Darcy Flour in Tide Embankment (호안제체에서 Non-Darcy 흐름해석)

  • Choi, Hung-Sik
    • Journal of Korea Water Resources Association
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    • v.37 no.2
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    • pp.87-96
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    • 2004
  • The simulation results using i- V relationship of non-Darcy flow through tide embankment by Li et al.(1998) agree well to the observed data. The use of i- V relationship is applicable to the engineering practice and the correct input of porosity is necessary. The non-Darcy flow based on the pipe flow and Taylor's definition for mean hydraulics radius in rockfill material is applicable to the block and caisson materials. The correct calculation of flow through tide embankment enables the accurate calculation of velocity at final closing gap and the prediction of inner water level after tide embankment construction as well.

Immunocytological Studies for the Degeneration of Cranial Myotomes in Xenopus laevis (무미 양서류 Xenopus laevis의 Cranial Myotomes Degeneration에 대한 면역 세포학적 연구)

  • 이상훈;이진표;정해문
    • The Korean Journal of Zoology
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    • v.33 no.1
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    • pp.94-102
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    • 1990
  • In Xenopus laevis the cranial myotomes, W, X, Y and Z represent transient embry-specific structures since they undergo reduction" in the later stage of development. An extensive set of expertmental studies was undertaken in order to discriminate whether the cranial myotomes perform a programmed autonomous death or reduction" by the influence from surrounding tissue such as otic vesicle. Removal of the neighboring otic vesicle did not affect WXYZ degeneration. Grafting the otic vesicle to novel site along the somite file did not induce local myotome degenration. When anterior-most somitic primordia were relocated to the trunk somite region, they underwent reduction eventually. Likewise, with the transplantation of posterior somite forming region into the anterior, autonomous differentiation pafferns were observed. And the cullture of presumptive somitic tissue revealed that WXYZ might degnerate like in vivo. It is therefore concluded that the cranial myotomes of Xenopus laevis exhibit an autonomous cell death during later embryogenesis.yogenesis.

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An Inverse Dynamic Model of Upper Limbs during Manual Wheelchair Propulsion (수동 휠체어 추진 중 상지 역동역학 모델)

  • Song, S.J.
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.7 no.1
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    • pp.21-27
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    • 2013
  • Manual wheelchair propulsion can lead to pain and injuries of users due to mechanical inefficiency of wheelchair propulsion motion. The kinetic analysis of the upper limbs during manual wheelchair propulsion needs to be studied. A two dimensional inverse dynamic model of upper limbs was developed to compute the joint torque during manual wheelchair propulsion. The model was composed of three segments corresponding to upper arm, lower arm and hand. These segments connected in series by revolute joints constitute open chain mechanism in sagittal plane. The inverse dynamic method is based on Newton-Euler formalism. The model was applied to data collected in experiments. Kinematic data of upper limbs during wheelchair propulsion were obtained from three dimensional trajectories of markers collected by a motion capture system. Kinetic data as external forces applied on the hand were obtained from a dynamometer. The joint rotation angles and joint torques were computed using the inverse dynamic model. The developed model is for upper limbs biomechanics and can easily be extended to three dimensional dynamic model.

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Tidal Propagation in the Keum River (금강 感趙구간의 조석전파)

  • 최병호;안원식
    • Water for future
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    • v.18 no.1
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    • pp.67-73
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    • 1985
  • Tidal propagation in the Keum River has been routinely handled by numerical integration of the long fravity wave equation by Dronkers. The dynamic equations include non-linear terms thereby reproducing the shallow water tides. The model was used to compute tidal distribution of the Kum River for aveage spring, mean, neap tidal conditions and further utilised to investigate the waterlevel response within tidal reaches by combined tide and flood discharge effects. The objective of this initial study is to investigate the tidal dynamics of the lower reaches of the Keum River under the condition of before-cross-channel barrage construction.

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Naupia Development of Nitocra spinipes Boeck, 1865 (Copepoda, Harpacticoida, Ameiridae) Reared in the Laboratory (요각류, Nitocra spinipes Boeck, 1865의 노우플리우스 유생 발생)

  • 최경화;김창현
    • Journal of Life Science
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    • v.11 no.5
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    • pp.470-475
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    • 2001
  • The larvae of Nitocra spinipes Boeck, 1865 were reared at 2$0^{\circ}C$ and 33.3$\textperthousand$ in the laboratory. These species passed through six nauplii stages and the first copepodid appeared in 8-10 days. Nauplii of N. spinipes is distinguished by characteristics of the appendage setation and caudal setae. The number of segments of antennal and mandibular exopodites in harpacticoida is discussed within familial level.

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Development of Flood Routing Model in the Ara Waterway Applying HEC-RAS (HEC-RAS를 적용한 아라천 홍수위산정모형 구축)

  • Noh, Joon-Woo;Lee, Sang-Jin;Park, Myung-Ki;Shim, Myung-Geunl
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.409-413
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    • 2012
  • 아라뱃길은 다양한 수리구조물의 조작을 통하여 선박운항 및 홍수처리가 이루어지며 유입홍수량, 한강수위, 그리고 서해조위 변동과 연계하여 귤현보, 서해배수문, 체절수문, 그리고 배수펌프장 등의 대표적인 구조물 운영을 지원할 수 있는 홍수위 계산모형이 필요하다. HEC-RAS모형은 이러한 수리구조물 운영을 효과적으로 경계조건으로 반영할 수 있는 장점이 있다. 특히 서해배수문의 경우 조위변동과 내수위를 동시에 고려한 수문운영이 필요하여 Rule-script기능을 적용하여 이러한 특성을 반영하였다. 모형의 검보정을 위하여 2010년 9월 홍수사상을 적용하였으며 모형으로부터 계산된 수위값과 관측된 수위값이 서로 잘 일치함을 확인하였다.

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