• Resources Recycling
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• v.28 no.3
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• pp.45-52
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• 2019

$TiO_2$ as a raw material for producing titanium can be produced by carbon reduction of natural ilmenite ores over 1823 K and acid leaching of the obtained titanium-rich slag. However, the conventional process can cause very high energy consumption and a large amount of leaching residues. In the present study, we proposed the sulfurization of $FeTiO_3$ with $Na_2SO_4$ at temperatures below 1573 K, which can separate Fe in $FeTiO_3$ as the FeS based sulfide phase and Ti as the $TiO_2-Na_2O$ based oxide phase. This study is a fundamental study for sulfurization of $FeTiO_3$ to investigate the influence of reducing atmosphere, reaction temperature and the sulfur/Fe ratio on the separability and distribution behaviors of of Fe, Ti, and Na between the oxide phase and the sulfurized phase. At 1573 K and carbon saturation condition, the Fe can be separated from $FeTiO_3$ as Fe-C-S metal and a part of FeS, and the concentration of Fe in oxide decreased to 4 mass% after sulfurization.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.2
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• pp.205-213
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• 2019

The protective effect of Agrimonia pilosa var. (AP) extract on potassium dichromate ($K_2Cr_2O_7$)-induced cytotoxicity in cultured NIH3T3 fibroblasts, was examined by performing an XTT assay for the cell viability and antioxidative effects, such as lactate dehydrogenase (LDH) activity and superoxide anion-radical (SAR) scavenging activity. In this study, $K_2Cr_2O_7$ decreased the cell viability significantly in a dose-dependent manner, and the $XTT_{50}$ value was determined to be $37.5{\mu}M$, which was highly-toxic according to the Borenfreund and Puerner' toxic criteria. The antioxidant, butylated hydroxytoluene (BHT), increased remarkably the cell viability damaged by $K_2Cr_2O_7$-induced cytotoxicity in these cultures. With regard to the protective effect of the AP extract on $K_2Cr_2O_7$-induced cytotoxicity, AP extract produced a significant increase in cell viability and antioxidative effects as the inhibitory ability LDH and SAR scavenging ability. These findings suggest that oxidative stress is involved in the cytotoxicity of $K_2Cr_2O_7$, and the AP extract effectively protected the cells from $K_2Cr_2O_7$-induced cytotoxicity by antioxidative effects. These results suggest that natural resources, such as AP extract, may be a putative therapeutic agent for the diminution or treatment of cytotoxicity induced by heavy metallic bases, such as $K_2Cr_2O_7$ correlated with oxidative stress.

• Journal of Life Science
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• v.29 no.11
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• pp.1192-1199
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• 2019

The intention of this study was to confirm the possible use of an ethanol extracts of Nelumbinis Rhizomatis Nodus (NRN) as a cosmetic material. To this end, we extracted NRN with 70% ethanol and performed biological activity evaluation of whitening efficacy and wrinkle reduction. We performed cellular tyrosinase inhibition and melanin contents assay to check the whitening activity of NRN and carried out a toxicity evaluation of NRN via an MTT assay and the amounts of associated proteins that affect melanin production in a melanoma cell line (B16F10). And collagenase inhibitory assay was performed for the evaluation of anti-wrinkle of samples. In addition, a toxicity evaluation using an MTT assay and matrix metalloprotease (MMP-1) and procollagen synthesis inhibition by NRN were evaluated in a fibroblast cell line (CCD-986sk). Western blot results for the whitening activity evaluation revealed that the levels of two proteins related to melanin production, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2), were decreased in a dose-dependent manner. Moreover, collagenase inhibition activity at a concentration of $500{\mu}g/ml$ NRN by measuring epigallocatechin-3-gallate (EGCG) was increased by more than 80% compared to the control group. Meanwhile, procollagen synthesis was reduced by 68.8% in the UVB-induced CCD- 986sk cells group whereas collagen synthesis recovered by 80.2% with $25{\mu}g/ml$ NRN. The MMP-1 expression rate showed 20.2% reduction at $25{\mu}g/ml$. The results of the experiments verified the whitening and wrinkle suppression effects of NRN and confirmed that it could be used as a safe natural cosmetic material in the future.

• Food Science and Industry
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• v.53 no.1
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• pp.69-83
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• 2020

For the development of the chitosan industry in Korea, the catch of red snow crabs caught on the east coast is rapidly decreasing. Therefore, it is urgent to develop raw materials that can replace the red snow crab as the top priority to solve the supply and demand problems, as well as wastewater treatment costs account for a large proportion of the cost of chitosan. In order to solve the problems, continuous research on biological extraction methods such as enzymatic extraction and microbial fermentation will increase production efficiency and lower unit cost as well as chemical extraction methods. Further efficient manufacturing method can be established. Establishing of novel techniques is indispensable for production of high-purity chitosan and the ability to regulate and separate the molecular weight, as well as joint research with industry, academia and research institute for the research and development of high-functional chitosan derivatives.

• Korean Journal of Plant Resources
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• v.35 no.2
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• pp.362-371
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• 2022

This study was conducted to analyze the fragrance characteristics of Cymbidium 'Saelbit' and 'Midan' according to floral organs. As test materials, full bloom flowers were divided into four organs: sepal, petal, labellum, and column. Using the gas chromatography (GC) based electronic nose, fragrance patterns, intensity, and volatile components were analyzed. Principle component analysis (PCA) and discriminant factorial analysis (DFA) plots by electronic nose data showed that volatiles of both cultivars have a distinct difference in fragrance patterns according to the floral organs, and the value of fragrance distance and pattern discrimination index (PDI) between samples was significantly high between control and sepals in both cultivars. Among the main fragrance components, several components including nootkatone were detected in both cultivars and all floral organs. However, few components such as decane were found in specific cultivar or floral organs. These results will provide useful information to select suitable materials with desired fragrance and to enhance the utilization of domestic Cymbidium cultivars. In addition, considering the recent negative perception of artificial ingredients and the growing demand for natural materials, continuous researches on scent properties of promising cultivars are required.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.1
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• pp.5-12
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• 2021

In order to extract the essential oil contained in the Elsholtzia splendense Nakai, a fully-bloomed individual was collected and the living body was used for the experiment. Plants were divided into 0.7kg of flowers and 1.5kg of leaves + stems, and extracted by parts and used for scent pattern analysis, and extracted after adding 5kg of outpost for the analysis of essential oil components. Essential oil extraction was performed using the SDE method improved by Schultz et al. (1977), and the extraction time was limited to 1 hour after the material started boiling. The extracted essential oil component was analyzed for fragrance pattern analysis using an e-nose, and the results of analyzing the substance of the essential oil component by GC-MS are as follows. 1. As for the fragrance pattern analysis, in the case of essential oils extracted from flowers, the scent quality was the best at 10-20 minutes, and the scents extracted from stems and leaves were somewhat of poor scent quality, but the fragrance was good at 10-40 minutes. The intensity of scent was the strongest in 10-20 minutes, and the intensity of incense was high even in 30-40 minutes. The scent extracted from the stems and leaves was generally not strong, but appeared high in 10 to 20 minutes. 2. There were 40 kinds of essential oils contained in Elsholtzia splendense Nakai oil. Among them, Mequinol, Benzene, 1,2,3,4-tetramethyl, Elsholtziaketone, and Dehydroelsholtziaketone were identified.

• Journal of Convergence for Information Technology
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• v.11 no.5
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• pp.199-205
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• 2021

This study was done to evaluate the dermatoxicity of zinc sulfate (ZnSO4) and the protective effect of Rubus parvifolius L. (RP) extract on cytotoxicity of ZnSO4, mordant in cultured SK-MEL-3 cells. For this study, it was done an antioxidative effect as DPPH-radical scavenging ability as well as the diminutive ability of total melanin with cell viability. ZnSO4 significantly decreased cell viability in dose-dependently, and it was mid-toxic. The ascorbic acid significantly increased cell viability damaged by ZnSO4-induced cytotoxicity. In the protective effect of RP extract on ZnSO4-induced cytotoxicity, RP extract significantly increased cell viability compared with ZnSO4-treated group, and also it showed both the DPPH-radical scavenging ability and the decrease of total amount of melanin. From these findings, the cytotoxicity of ZnSO4 is correlated with oxidative stress, and also RP extract effectively protected ZnSO4-induced cytotoxicity via antioxidative effect such as DPPH-radical scavenging ability with the whitening effect by the decrement of total amount of melanin. Conclusively, the natural ingredients like RP extract may be a useful agent for the improvement of antioxidative and whitening effects

• Journal of the Korean Institute of Gas
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• v.26 no.4
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• pp.58-63
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• 2022

With the emergence of environmental problems caused by fine dust worldwide, LNG, which is cheaper and less pollution than diesel, is attracting attention as the next generation energy of automobiles and is expanding its supply. However, it is difficult to operate smoothly due to the lack of infrastructure for LNG charging stations in Korea and the limited size of containers that can be installed according to regulations. In Korea, research and development on the contents of containers for the smooth operation of natural gas vehicles are underway, but there is a problem that the container directly receives the impact of the vehicle collision and explodes, causing a major disaster. Therefore, in this study, the safety of the container was verified by deriving the strain and stress values through ANSYS Explicit Dynamics analysis. As a result, a maximum stress of 565.37MPa occurred in the container, and it is expected that plastic deformation will occur as it exceeds the yield stress of STS304 used as a material for the container, which is beyond 505MPa. When an impact caused by a collision between a vehicle and a container is applied, it is considered necessary to design a support or reinforcement because the container may be damaged or defective.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.4
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• pp.298-306
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• 2022

This study sought to evaluate the mechanism of cellular injury caused by ferrous sulfate (FeSO₄) and the protective effects of Parnassia palustris L. (PP) extract against FeSO₄-induced cytotoxicity of cultured C6 glioma cells. FeSO₄ is known to cause neurotoxicity and induce Parkinson's disease. The antioxidative effects of PP, such as superoxide dismutase (SOD)-like and superoxide anion-radical (SAR)-scavenging activities, as well as effects on cell viability, were studied. FeSO₄ significantly decreased cell viability in a dose-dependent manner and the XTT₅₀ value, the concentration of FeSO₄ which reduced the cell viability by half, was measured at 63.3 μM in these cultures. FeSO₄ was estimated to be highly cytotoxic by the Borenfreund and Puerner toxicity criteria. Quercetin, an antioxidant, significantly improved cell viability, damaged by FeSO₄-induced cytotoxicity. While evaluating the protective effects of the PP extract on FeSO₄-induced cytotoxicity, it was observed that the extract significantly increased cell viability compared to the FeSO₄-treated group. Also, the PP extract showed superoxide dismutase (SOD)-like and superoxide anion radical (SAR)-scavenging activities. Based on these findings, it can be concluded that FeSO₄ induced oxidative stress-related cytotoxicity, and the PP extract effectively protected against this cytotoxicity via its antioxidative effects. In conclusion, natural antioxidant sources such as PP may be agents useful for preventing oxidative stress-related cytotoxicity induced by heavy metal compounds such as the FeSO₄, a known Parkinsonism inducer.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.28-29
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• 2021

애기똥풀(Chelidonium majus L. subsp. asiaticum H.Hara)의 지상부는 백굴채라고 불리며 항암, 항균 등의 효능을 가지고 있어 예로부터 약용으로 이용되었다. 그러나 천연 약용소재로 개발가치가 높음에도 불구하고 실생묘의 대량 확보를 위한 종자번식 관련 연구가 전무한 실정이다. 본 연구에서는 애기똥풀의 발아 및 휴면 특성을 분석함으로써 추후 종자번식을 위한 기초자료를 제공하기 위해 수행되었다. 실험재료로 사용된 애기똥풀 종자는 2019년 6월 경상북도 봉화군에서 채종하였으며 정선 후 건조상태로 실온(25℃)에 보관하였다. 종자의 1000립중은 0.57±0.01g이며, 배종비는 0.21±0.12로 조사되어 형태적으로 미성숙한 배를 가지고 있다. 종자의 활력은 테트라졸륨 검정을 통해 79%로 확인되었다. 종자의 기본 함수율은 7.64±0.25%였으며, 침지 2일 차에 최대함수율(32.6±0.34%)에 도달하였다. 초기 발아검정은 25/15℃ (light/dark, 16/8h)챔버에서 명조건과 암조건으로 구분하여 30일간 진행하였으며, 암조건은 알루미늄 호일로 광을 완전히 차단하고 최종 발아율만 조사하였다. 발아율을 조사한 결과, 명조건은 0.75±0.80%, 암조건은 5.50±1.55%로 조사되어 형태적인 휴면과 생리적인 휴면을 모두 갖는 것으로 판단하였다. 애기똥풀의 생태적인 환경을 고려하여 12주의 Summer temperature(S12, 25/15℃, 16/8h)로 층적하고 연속하여 Autumn temperature(A, 15/10℃, 16/8h)를 4주(A4)와 8주(A8)로 달리하여 종자의 휴면타파를 유도하였다. 층적 후 발아율은 모두 증가되었으며, S12-A4(54%)는 S12-A8(24.5%)에 비해서 휴면타파에 효과적이었다. 휴면타파 효과의 증진을 위해 층적처리한 종자를 500ppm의 GA₃와 GA₄₊₇에 각 24시간동안 4℃에서 침지시킨 뒤 동일한 조건에 파종하였다. GA₃가 처리된 S12-A4는 발아율이 82.0%, S12-A8는 30.8%로 유의적으로 증가하였으나 GA₄₊₇ 처리는 S12-A4(48.5%)와 S12-A8(21.3%)에서 발아율 향상 효과가 없었다. 결론적으로 애기똥풀의 휴면타파를 위해서 12주의 Summer temperature와 4주의 Autumn temperature 변온층적과 추가 GA₃ 처리가 휴면타파를 위한 효과적인 처리방법으로 판단된다.