• Korean Journal of Organic Agriculture
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• v.21 no.2
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• pp.219-232
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• 2013

The relevant main constituents of rosemary are compound of a vast number of polyphenolics, including carnosic acid, carnosol, rosmarinic acid, and ursolic acid. Recently, phenolic compounds of plant origin have attracted considerable attention due to their beneficial functional and nutritional effects including antioxidant and antibacterial activity. This study was carried out to investigated effect of carnosic acid on pH, thiobarbituric acid reactive substance (TBARS), volatile basic nitrogen (VBN), 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity, and total bacteria counts in white sausage during the storage at room temperature. Addition of carnosic acid was decreased of pH, TBARS, VBN, and enhanced antioxidant and antibacterial activity in white sausage. These activities increased with increasing concentrations of carnosic acid. Furthermore, the potent antibacterial activities of carnosic acid against pathogenic bacteria (Escherichia coli KCCM 11234 and Salmonella enteritidis KCCM 12021) were measured. Carnosic acid showed noticeable inhibitory effects on E. coli and S. enteritidis. In conclusion, carnosic acid might be used as a natural preservative in white sausage.

• Korean journal of food and cookery science
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• v.15 no.2
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• pp.121-126
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• 1999

In order to develop a natural food preservative, freeze-dried dandelion (Taraxacum platycarpum D.) was extracted with 99.5% (v/v) methanol, and its antimicrobial activity was investigated. The methanol extract at the concentration of 2,000 $\mu\textrm{g}$/ml completely inhibited the growth of S. aureus. When the solutions of dandelion at 1%, 3% and 5% were added to noodles and rice cakes, less microbial growth was observed compared with the control roup. For the noodles, the 3%-added group was the most superior in terms of color and moistness, and the 1%-added group gained the highest grade in terms of chewiness and overall quality. For rice cakes, the 3%-added group was evaluated to be the favorite.

• KSBB Journal
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• v.9 no.5
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• pp.506-511
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• 1994

Antimicrobial activity of methanol extract and fraction from mugwort leaves(Artemisia princeps val. orientalis) was investigated for the screening of natural antiwucroblal components. By using agar diffusion method, ethyl acetate(EtOAc) layer fractionated from methanol extract of mugwort leaves showed the highest inhibitory effects against tested microorganisms. The ortho-coumaric acid(200∼600ppm) isolated from EtOAc layer showed strong antibacterial activities for Bacillus subtilis and Salmonella typhimurium. As derivatives of o-coumaric acid, antibacterial activity of para-coumaric acid was 1.2∼1.7 fold higher than that of o-coumaric acid. Three types of coumaric acids strong inhibited the growth of B. subtilis in the culture medium. Growth of S. tyhimurium, P. aeruginosa and S. aureus were effectively inhibited by o-, m- and p-coumaric acids, respectively. Minimum inhibitory dose of p-coumaric acid for B. subtilis was $\100∼200mu\textrm{g}$/paper disk.

• Journal of Convergence for Information Technology
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• v.10 no.4
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• pp.104-114
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• 2020

We investigated the best antibiotics using blending oils after screening 11 kinds of essential oil known as antibiotics from plants. The minimum inhibitory concentration (MIC) and the minimum killing concentration (MBC) were found to be essential for essential oils B and E to inhibit target bacteria. All gram-positive bacteria containing S. aureus used in this experiment were shown highly antibiotic activity. And only A. baumanii in gram-positive bacteria and C. albicans in fungi were shown highly antibiotic activity. The essential oils used in our experiments showed better antibiotic activity compared to major studies using natural antibiotics with excellent antibiotic activity and essential oils from natural medicine. It is not known what mechanism of antimicrobial activity the essential oil used in the test has, but it is interpreted as a synthetic inhibitory mechanism of cell wall compared with other previous studies. From these results, it is expected that some substances or functional products with antibiotic activity will be developed.

• Korean Journal of Agricultural Science
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• v.17 no.1
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• pp.52-64
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• 1990

As a search for natural antioxidants, antioxdative fractions in Pueraria roots were extracted, identified using column chromatography, thin layer chromatography or high performance liquid chromatography. The components which have most effective antioxidative activities were further identified by IR and GC-MS. Separated antioxidative components were then added to four different oils to examine their antioxidative activities. Yield of extract obtained from pueraria root powder by solvent extraction using four step solvent systems was 2.54%. Antioxidative activity of the extracts was as effective as that of 100 ppm ${\delta}$-tocopherol addition, when 0.1% of the extracts was added to linoleic acid. The strongest antioxidative component of methanol extract of pueraria root was identified as puerarin. Aunioxidative activity of puerarin on lard was more effective than ${\alpha}$-tocopherol, but less effective than ${\delta}$-tocopherol. When the puerarin was added to edible oil and heat treated at $145^{\circ}C$, the acid value was lowest in lard and was highest in soybean oil. Antioxidative activity in terms of carbonyl value, thiobarbituric acid value and anisidine value was most high in palm oil and least in soybean and cottonseed oil.

• Journal of the Korean Applied Science and Technology
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• v.31 no.1
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• pp.130-135
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• 2014

To develop a natural antioxidant and anti-hemolytic agents, we investigated the effects of ethanol extracts of Rubus coreanus Miquel (crude extracts). Crude extract was extracted with ethanol. Antioxidant activity of crude extracts was evaluated by employing two different assays, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity and super oxide dismutase(SOD)-like activity. Also, anti-hemolytic activity of crude extract was determined using $H_2O_2$-induced hemolysis in normal rat red blood cells (RBC) or plasma. The extracts obtained from crude extract dose-dependently increased the scavenging activity on DPPH-induced radicals and SOD-like activity. RBC oxidative hemolysis induced by $H_2O_2$ was significantly suppressed by the extracts of Rubus coreanus Miquel in a dose-dependent manner. These results suggest that crude extracts may have value as the potential antioxidant and anti-hemolytic medicinal plant.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.4
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• pp.822-829
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• 1999

Antimicrobial activity of dandelion(Taraxacum platycarpum D.) was investigated. Methanol extract of dried dandelion was fractionated to hexane, chloroform, ethylacetate, butanol and aqueous fraction. Ethylacetate fraction among these fractions showed the highest inhibitory effect on the microorganisms such as B. subtilis, L. monocytogenes, S. aureus, E. coli and V. parahaemolyticus at $500\mu\textrm{g}/disc$. Ethylacetate fraction was further fractionated into 13 fractions by silica gel column and thin layer chromatography(TLC). The results showed that ethylacetate fractions No. 4, 5 and 6 had high antimicrobial activity. These were mixed again, re separated and five fractions were obtained. Among them, No. 2 fraction had the highest inhibitory effect on the microorganisms, which was then separated into six fractions. In the 3rd fractionation, No. 3 fraction was identified as benzoic acid by HPLC, $^{1}H-NMR$ and GC MS.

• Journal of dental hygiene science
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• v.4 no.2
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• pp.81-84
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• 2004

Gingival hyperpigmentation may cause esthetic problems and embarrassment. Especially in patients with a gummy smile. Melanin pigmentation is related to estiologic factor such as hormon, systemic factor, drug, smoking and gingival inflamation. During our search for new inhibitory components on melanogenesis from natural resources, MeOH extracts of more than 100 higher plants were tested for the inhibitory effect on melanogenesis in cultured B-16 mouse melanoma cell lines, and methylene chloride soluble part extract of Cnidii Rhizoma MeoH extraction was found to have potent activity. Cnidii Rhizoma, the root of Cnidium officinale Makino (Umbelliferae), is used for the treatment of abdominal pain, arthralgia, headache, hypertension, intestinal colic and for menstrual disorders and uterine cramps for its anti-blood stagnation effect. Two compounds were isolated and their chemical structures were determined as linoleic acid methyl ester(1), 1,3-dilinoleoyl-2-stearoyl glycerol(2), on the basis of physical and spectral data.

• Journal of Convergence for Information Technology
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• v.10 no.1
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• pp.243-250
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• 2020

Due to the unique sourness and sweet taste of Aronia, it is necessary to develop it for processing rather than raw. The antioxidant activity and cytotoxin about the aronia powder fermentation extract using lactobacillus are verified. In the case of total polyphenol content, the content of non-fermented extract was 32.15 ㎍/mg fermentation extract, 43.08 ㎍/mg after fermentation, and the flavonoid content was 0.47 ㎍/mg in non-fermented extract and 0.44 ㎍/mg in fermented extract, which was similar to that of non-fermented extract. In the DPPH radical inhibition assay, 77.5% of the non-fermented extract and 89.1% of the fermented extract showed better activity than the non-fermented extract. Nitric oxide (NO) measurement showed a concentration-dependent inhibitory effect. From the above results, it was confirmed that the fermentation of Aronia powder could be utilized based on some antioxidant activities and the possibility of using it as a vegetable extract and fermented cosmetic material in the future.

• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.535-557
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• 1998

설악산국립공원은 1965년에 천연보호구역, 1970년에 국립공원 제 5호, 1982년 유네스코에 의해 생물권보존지역으로 지정된 지역이다. 우리 나라 국립공원에서는 물론 다른 자연보존지역에서도 찾아볼 수 없는 중요성이 강조된 지역이나 연간 400만명의 이용객이 집중되기도 하는 지역이다. 우리 나라 국립공원은 본래 관광개발을 위해 지정된 동기를 갖고 있어 자연생태계 및 자연경관도 중요하지만 현실적으로는 합리적인 이용도 중요하다. 그러나 지금까지 국립공원은 자연보존에 대한 관리는 도외시 한 채 이용객 집중에 의해 발생되는 도로, 시설물, 건축물, 각종 오염에 대한 대책마련도 제대로 하지 못하여 국립공원하면 국민은 '등산이나 하고 값싼 음식점과 여인숙 수준의 숙박시설이 있는 관광지'정도로 인식하고 있다. 지난 30년간 압축고도성장을 해 온 우리 나라는 국토의 자연환경이 수없이 훼손되고 또한 옥외 레크레이션활동의 증가로 환경오염이 계속 증가되었다. 설악산국립공원도 예외는 아니어서 저앗ㅇ부와 등산로 주변생태계, 계곡생태계 등이 오염되어 식물, 포유동물, 물고기, 곤충 등에 영향이 나타나고 있지만 대피소, 휴게소, 숙박시설을 중심으로 한 오염행위는 계속 늘어만 가고 있다. 이제 설악산국립공원은 거의 위기상황에 도달된 느낌이다. 이런 시기에 환경생태학회 국립공원분과위원회 회원들은 `96년 외설악 지역, `97년 내설악지역을 2년 동안 각 분야에 걸쳐 조사를 수행하였다. 지난 12년간 외부의 지원도 없이 연구비를 자체 조달하여 힘들게 국립공원 연구를 진행하여 온 일환으로 설악산국립공원 조사를 2년 동안 수행한 것이다. 이번 조사는 `96년 가을 북한공비침투로 많은 지역에 대한 출입이 통제되어 외설악연구의 일부가 누락되었음을 첨언한다. 본 글에서는 2년 동안 본 회원들이 조사한 내용에 의해 설악산 국립공원의 현황을 분석하고 관리개선 방향을 제시하였다.