• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.257-261
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• 1998

The repeated subcultures of in vitro plant materials in wasabi became highly vitrified and the capacity for multiple shoot formation from the vitrified plant materials was very low. In order to improve the quality of in vitro propagated planting materials, the experiments were carried out using culture vessels capped with membrane filter(MF). When vitrified shoots were cultured on MS medium with 0.2mg/L BA in the vessels with MF or without MF for 60 days, the shoots in the vessels with MF did not vitrified. In contrast, the shoots grown in the vessels without MF vitrified at 65%. The stomates of vitrified leaves were circular and inflated, whereas those of normal leaves acclimatizated in the vessels with MF were ovate in shape. The hardened shoots were also cultured on MS media without sucrose containing 0.01mg/L IBA in vessels with(photoautotrophic culture) or without(control) MF. Sucrose was necessary for survival of the in vitro plantlets in the vessels without MF. After 20 days of culture, the shoots in the vessels without MF on the sucrose-free media turned yellow and died. But the shoots in the vessels with MF in the sucrose-free media produced a lot of roots. When shoots were cultured on MS medium with 2% sucrose containing 0.01mg/L IBA in the vessels with(photomixotrophic culture) or without(heterotrophic culture) MF, best growth occured in photomixotrophic culture.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.65-71
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• 2013

This research was conducted to evaluate the influence of $NO_3{^-}:NH_4{^+}$ ratios in fertilizer solution on the vegetative growth and fruit yield of hot pepper (Capsicum annuum L.) through pot cultivation. The Hoaglad's solution was modified to contain various $NO_3{^-}:NH_4{^+}$ ratios such as 100:0 (A), 73:37 (B), 50:50 (C), 27:73 (D), 0:100 (E), and no nitrogen (F). Plants were transplanted into root substrates and the modified solutions were applied as plant needed in plastic house. There were no statistical significances among the treatments from A through D in the fresh and dry weights, and number of leaves 31 days after transplanting, but elevation of $NH_4{^+}$ ratios in the solution decreased the fresh fruit weight 62 days after transplanting with statistical differences. In the results of inorganic element analysis based on the dry weight of fully expanded mature leaves, N and P contents as well as micro cations such as Fe, Mn, Zn, and Cu increased as $NH_4{^+}$ ratios were elevated 62 days after transplanting. However, those of macro cations such as K, Ca, and Mg resulted in decreasing tendency. The elevation of $NH_4{^+}$ ratios in fertilizer solution resulted in the increase of EC and total N concentrations ($NO_3{^-}+NH_4{^+}$), but this decreased the pH as well as Ca and Mg concentrations in soil solution 62 days after transplanting. The K concentration in soil solution was the highest in the treatments of C and followed by D, B, E, and A. The above results indicate that the proper $NO_3{^-}:NH_4{^+}$ ratio in the nutrient solution is 73:27 (B) or 100:0 (A) and the B solution is proper for the vegetative growth and that of A is proper for reproductive growth stage.

• Journal of Bio-Environment Control
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• v.13 no.1
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• pp.26-32
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• 2004

Growth and yield of cucumber (Cucumis sativus L.) with arch training method were evaluated on several training heights, planting densities, and topping node orders. Cucumber cultivar 'Eunsung-baekdadagi' was planted on 9 June with three training heights of 1.5, 1.8, and 2.1 m, there planting densities of 90${\times}$40, 90${\times}$50, and 90${\times}$60 cm, and five topping node orders of 20th, 30th, 35th, and nontopping. The plot of 2.1 m training height resulted in the higher sun-scald fruit rates due to the higher temperature above 37$^{\circ}C$ in the upper space of plastic house. The plot of 1.8 m arch training height showed higher fruit setting and marketable yield rate compared to the other training heights. The maketable yield rate with 1.8 m height arch training was 102,691 kg ${\cdot}$ $ha^{-1}$, 21% higher value than that of 1.5 m. Powdery mildew incidence increased with the increase of planting density. Lower LAI were shown depending on the higher topping node order. Lower light transmission ratio was shown in the higher planting density plots, might be due to the crowded stems and leaves inside those plots. Fruit setting rate was also higher in main stems rather than in lateral ones. Marketable yie이 in 90${\times}$50 cm planting distance with 35th node topping treatment was 98,311 kg ${\cdot}$ $ha^{-1}$, 5% higher than that 90${\times}$40 cm planting distance with 30th node topping treatment. Thus the 1.8 m of training height, 90${\times}$50 cm of planting distance, and 35th node topping was evaluated for the effective cultivation condition in arch training of cucumber in highland.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.438-446
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• 2019

The interest in mini watermelon (Citrullus lanatus) with small fruits weighing 2-3 kg has increased by the increasing trend in one-person households and consequent tendency to consume small meals. Watermelon grafting onto cucurbit rootstocks is a very effective way to control soil-borne diseases, such as Fusarium wilt; however, this practice negatively impacts the fruit quality. This study was conducted to investigate the growth, fruit set, and fruit quality of mini watermelon grafted onto wild watermelon accessions (Citrullus spp.) resistant to Fusarium wilt. Five watermelon accessions (Galactica, IT 208441, PI 482322, PI 500303, and PI 593358) were evaluated as rootstocks for the mini watermelon "Ministar". Non-grafted "Ministar" and "Ministar" grafted onto "Shintozwa" (Cucurbita maxima D. C. moschata D.) or "Bullojangsaeng" (Lagenaria leucantha) were used as controls. The roots of the transplants grafted onto "PI 593358" and "Shintozwa" weighed more than those on other rootstocks. Additionally, the transplants on "PI 593358" showed better growth and fruit set in the field than the other transplants. However, the total soluble solid contents and fruit quality indices of the transplants on "PI 593358" and "Shintozwa" were lower, whereas the total fruit quality index of those on "PI 482322" was higher. Thus, the wild watermelon accessions tested can potentially be used as basic germplasm for developing watermelon rootstocks instead of cucurbit rootstocks. The most promising accession for this purpose was found to be "PI 482322".

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.312-319
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• 2021

This study was conducted to investigate the effect of elevated carbon dioxide on the growth and physiological responses of peach 'Mihong' (Prunus persica). We simulated three different carbon dioxide conditions based on climate change scenarios RCP 8.5 in the sunlight phytotron rooms from April 22 to July 6, 2020; 400 µmol·mol^-1(present condition), 700 µmol·mol^-1 treatment(expecting carbon dioxide concentrations in mid-21st century), 940 µmol·mol^-1 treatment (expecting carbon dioxide concentrations in late 21st century). The average of maximum photosynthesis rate at 700 µmol·mol^-1(16.06 µmol·CO₂·m^-2·s^-1) was higher than those at 400 µmol·mol^-1(14.45 µmol·CO₂·m^-2·s^-1) and 940 µmol·mol^-1(15.96 µmol·CO₂·m^-2·s^-1) from May 22 to July 2. However, stomatal conductances at 700 µmol·mol^-1 and 940 µmol·mol^-1 were lower than those at the control. Also, the carbon dioxide saturation point in all treatments was reduced from 1,200 µmol·mol^-1 in the early stage of growth to 600-800 µmol·mol^-1 in the late stage of growth. The stomatal densities were decreased as carbon dioxide increased. The shoot lengths were decreased while the carbon dioxide was increased, but the increase of trunk diameter and leaf areas, shoot numbers were not statistically different. The fruit weight at 700 µmol·mol^-1(152.5 g) was higher than those at the control(141.8 g) and 940 µmol·mol^-1(147.4 g). The soluble solids were higher at 700 µmol·mol^-1, 940 µmol·mol^-1 compared to the control. These results suggest that a carbon dioxide elevated to 700 µmol·mol^-1 in the future may give a positive effect on the yield and fruit quality of peach 'Mihong' while a carbon dioxide elevated above 940 µmol·mol^-1 may affect negatively such as early senescence and loss of fruit set.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.263-270
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• 2021

This experiment was carried out to determine the effect of CaCl₂ foliar spraying on the inhibition of blossom-end rot (BER) in hot summer paprika (Capsicum annum L. 'Special') cultivation. The effect of CaCl₂ application was examined by the foliar application based on different fruit size, frequency, and spraying time. Also, it was investigated the occurrence and alleviation effect of BER symptom. Foliar application of CaCl₂ (Ca 0.4%) was conducted by treating a fruit and leaf at 4 to 9 nodes above the crown flowers of each plant with 350 mL per week from June 3 to July 1. When the CaCl₂ was sprayed at 7-day intervals for 4 weeks, the Ca content was the lowest in the fruit harvested with BER symptom in 11 to 20 mm of fruit width (FW). Four different regions in both BER symptom and normal fruits showed significant differences of Ca content, the highest was in pedicel, followed by stem-end, middle, and blossom-end. The Ca content increased sharply in normal paprika with 31-40 mm FW, in which Ca content was 78% higher than that of BER. Ca content in the middle and blossom-end of paprika over 21 mm in FW ranged 19.8% to 28.8% in normal fruits and 15.7% to 18.5% in BER, respectively. The incidence of BER increased rapidly by more than 60% in fruits with 31-40 mm FW. While there was no difference in fruit weight among the FW treatment, marketable yield rate was highest in the 21-30 mm FW, and the sugar content was high in the 11-30mm FW. When CaCl₂ was applied three times for 7 days to a paprika having a 21- 30 mm FW, the cell wall-bound (CWB) Ca content was the highest and the rate of BER was lowest with 6.3%. After 10 days of CaCl₂ foliar spray treatment, the CWB Ca content of paprika increased by 2.9 to 3.5 times compared to the control in all treatments. At 7 days after the CaCl₂ foliar spraying once a day at varying spraying time, the leaf burn observed from 9:30 a.m. to 17:00 p.m. and the proline content increased as the spraying time was delayed. Therefore, the CaCl₂ foliar spraying method for reducing of BER occurrence during paprika summer cultivation seems to be appropriate to spray 2-3 times at intervals of 3 days and before 8 a.m. at the time when the FW is 21-30 mm.

• Korean Journal of Heritage: History & Science
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• v.54 no.4
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• pp.90-111
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• 2021

Seojangdaeyajodo is a drawing of Jangyongyoung's military night training on February 12 (lunar leap month), 1795. Focusing on the Seojangdaeyajodo, the positions and roles of the low-ranking soldier belonging to Jangyongyoung, and the composition and characteristics of military uniforms for each role were examined. The results ascertained by the historical research on the military uniforms are as follows. Deungronggun, noeja, sunryeongsu and daegisu who were placed in front of the king's Seojangdae were the low-ranking soldiers belonging to Jangyongyoung. The soldiers who escorted the king around Seojangdae were lowranking soldiers belonging to Jangyongyoung. The military uniform of the deungronggun was consisted of a jeolrip, a black heopsu, red gweja, indigo jeondae, white haengjeon and black shoes. The low-ranking soldier's heopsu suggested that it could also be a sochangui. He carried a sword and a red lantern. Noeja were divided into a sinjeonsu and a jujangsu. The military uniform of the noeja was consisted of a Jujeolrip, a black heopsu, red gweja, indigo jeondae, white haengjeon, and black shoes. Sunryeongsu were divided into a sinsigisu and a younggisu. The military uniform of the sunryeongsu was consisted of a jeongeon, a black heopsu, red gweja, indigo jeondae white haengjeon and black shoes. He carried a sword and a red lantern. The military uniform of the daegisu was consisted of a jeongeon, a black heopsu, blue gweja, indigo jeondae, white haengjeon and black shoes. He carried a sword and a flag. The soldiers surrounding Seojangdae and the seongjeonggun defending the fortress were the Chogun. The military uniform of the chogun was consisted of a jeolrip, a black heopsu, houi, indigo jeondae, white haengjeon and straw shoes. Houi was applying the five directional colors: the east is blue, the west is white, the south is red, and the north is black. He carried a sword and a gun. It was presented as an illustration of costumes that could produce contents by reflecting on these historical results. The basic principle of the illustration was to present the standards for 3D content production or actual production. Samples of form, color, and material according to the times and status were presented. The front, the side, and the back of each costume and the feature were presented, and the colors were presented in RGB and CMYK.

• Journal of International Area Studies (JIAS)
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• v.22 no.2
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• pp.217-244
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• 2018

As Chinese insurance market grows rapidly due to market reforms, China got recognition as second-largest insurance market in the world in 2016. However Korean insurance companies have had difficulties to grow in Chinese insurance market despite Chinese participation in the World Trade Organization (WTO) and the growth of chinese insurance market. The reason for the decline in Korean insurance companies is economic retaliation due to the deployment of THAAD in Korea. However, this is not a fundamental reason. The underlying cause of the effects of economic retaliation is the result of insufficient negotiations in Korea-China FTA services sector. In Service sector of Korea-China FTA, the level of concession between Korea and China differs greatly, when comparing China and South Korea's FTA. In addition, it has only been a few years since China collected the position of the WTO Doha Agenda, which currently deals with concessions on intellectual property rights and services. More important is that the Korea-China FTA service sector, as it stopped short of being protected by the most-favored-nation state treatment level when Korean insurance companies entered China. Further negotiations to supplement the weak points in the Korea-China FTA service sector were promised in December 2017, but international tensions over THAAD deployment made it impossible to hold such negotiations. However, due to changes in international affairs in 2018, the first additional negotiation was decided and held. This paper implicates strategies of further negotiation between Korea and China for service and investment chapter. Consequently, the aim of this paper suggests directions how to re-enter Chinese insurance market to Korean insurance companies.

• Korean Journal of Environment and Ecology
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• v.33 no.4
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• pp.422-439
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• 2019

The rear edge population is considered to have low genetic diversity and high risk of extinction according to a highly isolated distribution. However, the rear edge population is observed to have persisted for an extended period despite the low genetic diversity. As such, it is necessary to understand the ecological process involved in the persistence of the population. Viola mirabilis L. in Korea is considered the rear edge population from the perspective of the worldwide distribution. We surveyed the distribution range of V. mirabilis, which shows the isolated distribution in the central area of Korea, to find out the factors of its persistence. Next, we investigated and accessed the vegetational pattern of habitats, soil environment, phenology, self-compatibility, population structure, and extinction risk factors observed in the distribution area. V. mirabilis was distributed in the understory of the deciduous forest, planted forest of the deciduous conifer and deciduous broad-leaved trees, shrubland, and grassland in the limestone area. We also observed the re-establishment of seedlings in the population, and most of them showed a stable population structure. For chasmogamous flowers, the visit by pollinators has a significantly positive relationship with the production of fruits. However, we found that the production of the cleistogamous flowers was more numerous in all studied populations and that only the cleistogamous flowers were produced despite a more substantial plant size in some populations. The plant size was more related to the production of the cleistogamous flowers than that of the chasmogamous flowers. Accordingly, the cleistogamous flowers significantly contributed to seedling recruitment in the population. We found that the production of the chasmogamous flowers and the cleistogamous flowers did not have a correlation with the factors of the soil analysis except for phosphoric acid. V. mirabilis showed the self-incompatibility characteristics most likely due to the production capability of the cleistogamous flowers. Potential extinction risk factors observed in the distribution area was included the development of limestone mine, the expansion of agricultural fields, and the construction of houses. Although V. mirabilis showed an isolated distribution in the limestone area in the Korean peninsula, it showed a diverse distribution in a wide habitat environment ranging from the grassland to the understory of the trees with relatively low canopy closure rate. Moreover, we concluded that the persistence of the population was possible if we can maintain the current state of multiple populations and stable population structure.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.6
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• pp.111-119
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• 2018

This study was carried out to investigate the growth characteristics and propagation methods of the Korean native Amelanchier asiatica, A. arborea, and A. alnifolia as fruit trees for gardens. Due to the lack of recent research on Amelanchier spp., their superficial classification is still unclear and the names are being used interchangeably. The results are obtained as follows : A. arborea and A. alnifolia were globular type multi-stemmed shrubs. A 20-year-old tree of A. asiatica was 7.8m in height, with a 5.2m crown width, with one trunk. As for the morphological characteristics, leaves of A. asiatica were oblong, with an acuminate of, 6.1cm and 3.6cm width, but A. arborea and A. alnifolia had acute obovate leaves. The leaf size of A. alnifolia was the largest among the three species. The flower size of A. asiatica was bigger than that of A. arborea and A. alnifolia. In addition, its petals and flower clusters were also the largest among the three species. The flowering of A. asiatica initiated on April 21 and then bloomed for a duration of 24 days in Osan, while that of A. arborea and A. alnifolia initiated flowering on April 12 and then bloomed for a duration of 22 days in the same location. The fruit of A. arborea and A. alnifolia were green on May 10~12, it changed into purplish red on May 24~26, and its matured on June 1~3. The duration of fruit persistence of A. arborea and A. alnifolia were 48~50 days. On the other hand, A. asiatica showed greenish fruit on May 20, it became red on September 4, and had fallen by October 3. The fruit size was the largest at 1.03cm of height and 1.12cm of diameter in the A. arborea, followed by the big berry of A. alnifolia and the smallest fruit in the native, A. asiatica. It was difficult to root due to the hardwood cutting of A. arborea at a 40% rate of rooting. In the softwood cutting, the rooting rate of A. arborea was increased by the treatment with concentrated IBA, especially at 5,000 and 7,000ppm. The optimum date for cutting was on June 27, when the rooting rate was more than 80%. The most effective method for rooting of A. arborea was rootone or 7,000 ppm IBA treatment on June 27 softwood cuttings, which showed a rooting rate of over 80%.