• Title/Summary/Keyword: 집락성

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Preservation Methods of Xanthomonas campestris pv. oryzae in Relation to Virulence and Colony-Type Variation (벼 흰빛잎마름병균의 균주보관에 따른 병원성 및 집락변이현상)

  • Hwang In Gyu;Cho Yong Sup
    • Korean Journal Plant Pathology
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    • v.2 no.3
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    • pp.150-157
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    • 1986
  • Effects of presservation methods on viability and virulence of Xanthosmonas compestris pv. oryzae were investigated. The incidence of colony-type variants from freeze-dried and deep-frozen cultures was also determined. The suspending medium for freeze-dried cultures containing $10\%$ sucrose and $1\%$ gelatin showed the highest viability, and the virulence was well maintained in the suspending medium containing $2\%$ dextrin, $0.5\%$ ascorbic acid, 0.5% ammonium chloride, $0.5\%$ thiourea, and $0.85\%$ NaCl. Serially transferred cultures became attenuated. Rough colonies which had wrinkled surface occurred at a frequency of $1.9\%$ to $15.8\%$ during freeze-drying and freezing. The rough colonies consisted of nonseptated filamentous cells and rod-shaped cells. The virulence of rough colonies was weak as compared with the normal colony type.

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A Study on the Randomized Response Technique by PPS Sampling (확률비례추출법에 의한 확률화응답기법에 관한 연구)

  • Lee Gi-Sung
    • The Korean Journal of Applied Statistics
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    • v.19 no.1
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    • pp.69-80
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    • 2006
  • In this study, we make an effort to find a method to acquire sensitive information when sensitive populations are consisted of several clusters that vary in size. We suggest and systemize the theoretical validity for applying RRT(Randomized Response Technique) to PPS(Probability Proportional to Size) sampling method and derive the estimate and it's variance of the proportion of sensitive characteristic of population by using the suggested method. We compare the efficiency of the suggested technique by two-stage equal probability sampling. We examine practical aspects of the suggested method of RRT by PPS sampling through field survey.

A study on design effect models for complex sample survey (설계효과모형 적용에 관한 연구)

  • Park, Inho
    • Journal of the Korean Data and Information Science Society
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    • v.25 no.3
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    • pp.523-531
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    • 2014
  • Design effect is often used in designing and planning sample surveys and/or in evaluating the efficiency of complex design features of the surveys. In this study, we applied Gabler et al. (2006)'s design effect model to 2013 Consumer behavior survey for food that was carried out by stratified two-stage sampling. Usability and adequacy of the design model to a real survey data are discussed and evaluated.

Development of Random fracture network for discontinuity plane (불연속면의 확률절리망 알고리즘의 개발)

  • Ko, Wang-Kyung
    • Journal of the Korean Data and Information Science Society
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    • v.11 no.2
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    • pp.189-199
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    • 2000
  • A major deficiency of laboratory testing of rock structure is that the structures are limited in size and therefore present a very small and highly selective sample of the rock mass from which were removed. In a typical engineering project, the samples tested in the laboratory represent only a very small fraction of one percent of the volume of the rock mass. In this paper, we calculate the representative orientation of the resultant vector, the measure of the degree of clustering, the volume of rock mass, the trace length of discontinuity spacing under underlying distributions. And we generate the random fracture networks using real data. We propose the calculating the trace length.

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Colony Size Distributions according to in vitro Aging in Human Skin Fibroblasts (피부 섬유모세포 노화에 따른 세포집락 크기의 분포)

  • Kim, Jun-Sang;Kim, Jae-Sung;Cho, Moon-June;Park, Jeong-Kyu;Park, Tae-Hyun
    • Radiation Oncology Journal
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    • v.17 no.2
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    • pp.158-165
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    • 1999
  • Purpose : To investigate the percentage of colonies wi1h16or more cells distribution of human skin fibroblast according to in vitro aging, and to evaluate the relationship between percentage of colonies with 10 or more cells and in vivo donor age in human skin fibroblast culture. Material and Method : C1, C2, C3a, and C3b human skin fibroblast samples from three breast cancer patients were used as subjects. The C1, C2, and C3a donor were 44, 54, and 55 years old, respectively. C3a and C3b cells were isolated from the same person. Single cell suspension of skin fibroblasts was prepared with primary explant technique. One hundred cells are plated into 100m1 tissue culture flask and cultured for two weeks. The colony size was defined as colonies with 16 or more cells. The cultured cell was stained with crystal violet, and number of cells in each colony was determined with stereo microscope at $\times$10 magnification. Passage number of C1, C2, C3a and C3b skin fibroblast were 12th, 17th, and 14th, respectively. Results : Percentage of colonies with 16 or more cells of skin fibroblast samples decreased with increasing in vitro passage number. In contrast, cumulative population doublings of skin fibroblast sample increased with increasing in vitro passage number. Percentage of colonies with 16 or more cells also decreased with increasing population doublings in human skin fibroblast culture. There was strong correlation with percentage of colonies with 16 or more cells and population doublings En C3a skin fibroblast sampie. At the same point of population doublings, the percentage of colonies with 16 or more cells of the young C1 donor was higher level than the old C3a donor. Conclusion : The population doublings increased with increasing in vitro passage number but percentage of colonies with 16 or more cells decreased. The results of this study imply that percentage of colonies with 16 or more cell is useful as a indicator of in vitro human skin fibroblast aging and may estimate the in vivo donor age.

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A Study on the Optimal Aggregation Interval for Travel Time Estimation on the Rural Arterial Interrupted Traffic flow (지방부 간선도로 단속류 통행시간 추정을 위한 적정 집락간격 결정에 관한 연구)

  • Lim Houng-Seak;Lee Seung-Hwan;Lee Hyun-Jae
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.3 no.2 s.5
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    • pp.129-140
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    • 2004
  • In this paper, we conduct the research about optimal aggregation interval of travel time data on interrupted traffic flow and verify the reliability of AVI collected data by using car plate matching method in RTMS for systematic collection and analysis of link travel time data on interrupted traffic flow rural arterial. We perform Kolmosorov-Smirnov test on AVT collected sample data and on entire population data, and conclude that the sample data does not represent pure random sampling and hence includes sample collection error. We suggest that additional review is necessary to investigate the effectiveness of AVI collected sample data as link representative data. We also develop statistical model by applying two estimation techniques namely point estimation and interval estimation for calculating optimal aggregation interval. We have implemented our model and determine that point estimate is preferable over interval estimate for exactly selecting and deciding optimal aggregation interval. Our final conclusion is that 5-minute aggregation interval is optimal to estimate travel time in RTMS, as is currently being used our investigation is based on AVI data collected from Yang-ji to Yong-in $42^{nd}$ National road.

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A Comparative Study on the Statistical Methodology to Determine the Optimal Aggregation Interval for Travel Time Estimation of the Interrupted Traffic Flow (단속류 통행시간 추정을 위한 적정 집락간격 결정에 관한 통계적 방법론 비교 연구)

  • Lim, Houng-Seok;Lee, Seung-Hwan;Lee, Hyun-Jae
    • Journal of Korean Society of Transportation
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    • v.23 no.3 s.81
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    • pp.109-123
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    • 2005
  • The goals of this paper are two folds: i) to evaluate whether the data collected by a license plate matching AVI equipment being operated on some segment of a national highway are suitable or not for use in travel time estimation of interrupted traffic flows; ii) to study the statistical methodologies to be used for the determination of the optimal aggregation interval for travel time estimation. In this study it was found that the AVI data are not representative because the data are collected on some selected lanes of a roadway where main traffic is thru-traffic and, thus the AVI data are different from those collected from all lanes in traffic characteristics. For the determination of the optimal aggregation interval for travel time estimation. two statistical methods. namely point estimation and interval estimation. were tested. The test shows that the point estimation method is more sensitive and gives more desirable results in determing the optimal aggregation interval than the interval estimation method. And it turned out that the optimal aggregation interval on interrupted traffic flows has been calculated as 5 minute and thus the existing aggregation interval. 5 minute is proper.

Influence Factors for Duration of Vancomycin Resistant Enterococci's Spontaneous Decolonization (반코마이신 저항 장구균의 자발적 집락 소실 기간에 대한 영향 인자)

  • Lee, Jae Sun;Kim, Dong Soo;Kim, Ki Hwan
    • Pediatric Infection and Vaccine
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    • v.22 no.1
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    • pp.16-22
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    • 2015
  • Purpose: The aim of this study was to identify the factors influencing the spontaneous decolonization period of vancomycin resistant enterococcus (VRE) species in pediatric patients. Methods: The medical records of patients presenting positive VRE cultures between January 2005 and November 2010 at a tertiary hospital in Seoul, Korea, were reviewed retrospectively. The subjects were divided into two groups according to the average number of days for decolonization (325 days). Clinical characteristics were compared between shorter VRE colonization patients (<325 days, n=41) and prolonged VRE colonization patients (>325 days, n=110). Results: There were 151 patients who had more than 1 year of follow up period or confirmed of VRE decolonization among patients who were identified with VRE. The average age at the time of initial VRE colonization was significantly younger in shorter decolonization group than in prolonged decolonization group (44.9 months vs 40.9 months, P =0.040). The prolonged decolonization group received more vancomycin treatments after VRE colonization in comparison with patients in shorter decolonization group (7.0% vs 27.2%, P =0.008). Conclusion: For the duration of VRE colonization, it was found that the initial age of acquiring VRE and use of antibiotics were important factors. Antibiotics should be used properly and precisely in order to treat infectious diseases and to control the colonization of antibiotic resistant bacteria.

Integrated calibration weighting using complex auxiliary information (통합 칼리브레이션 가중치 산출 비교연구)

  • Park, Inho;Kim, Sujin
    • The Korean Journal of Applied Statistics
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    • v.34 no.3
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    • pp.427-438
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    • 2021
  • Two-stage sampling allows us to estimate population characteristics by both unit and cluster level together. Given a complex auxiliary information, integrated calibration weighting would better reflect the level-wise characteristics as well as multivariate characteristics between levels. This paper explored the integrated calibration weighting methods by Estevao and Särndal (2006) and Kim (2019) through a simulation study, where the efficiency of those weighting methods was compared using an artificial population data. Two weighting methods among others are shown efficient: single step calibration at the unit level with stacked individualized auxiliary information and iterative integrated calibration at each level. Under both methods, cluster calibrated weights are defined as the average of the calibrated weights of the unit(s) within cluster. Both were very good in terms of the goodness-of-fit of estimating the population totals of mutual auxiliary information between clusters and units, and showed small relative bias and relative mean square root errors for estimating the population totals of survey variables that are not included in calibration adjustments.

Goodness of Fit and Independence Tests for Clustered Sample Data (집락표본자료에 대한 적합도검정과 독립성검정)

  • 南宮 坪;崔秉洙;李柱祿
    • The Korean Journal of Applied Statistics
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    • v.2 no.2
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    • pp.79-89
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    • 1989
  • Modified Pearson $X^2$ statistic is concerned. Moreover the four statistics(Pearson, Wald, modified sample design effects and reduction factor) are compared in one-stage sampling situation. In case of categorical of fit and independence tests for sample data above, it is shown that there is a significant behavior between Pearson $X^2$ and Wald statistic, but minor difference in modified statistics by simulation methods.

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