• Title/Summary/Keyword: 집단유전학

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Phylogeny of Bombyx mandarina inhabiting Korea analysing the isozyme and hemolymph protein polymorphism (동위효소와 체액단백질 분석에 의한 한국산 멧누에나방의 지역적 특성)

  • 이재만;김경아;노시갑
    • Journal of Sericultural and Entomological Science
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    • v.45 no.1
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    • pp.18-24
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    • 2003
  • B. mandarina of Korean population apparently differs B. mori in isozyme analysis. Fourteen polymorphism occurred B. mandarina not in B. mori at 6 isozymes, Bph, Bes, Amy-hc, Ies, Ict-D, Ict-E. Korean population has shared with the Korean native strain of B. mori in B genotype of Bes, F of Amy-hc, n of Ict-E, M and S of Ict-H. These 5 genotype were known that detection only Korean native strains of B. mori. Nei's genetic distance based on the genotype of isozyme and hemolymph protein using 4 populations of B. mandarina varied from 0.0350 to 0.0624. The distances of 0.0350 is between Jinju and Chilgok population and between Jinju and Kosung population has the largest distances, 0.0624. In genus of Bombyx, B. mandarina and B. mori, genetic distance varied from 0.3822 to 0.5074. Phylogenetic tree obtained using the subprogram UPGMA of NTSYS represented that Bombyx devided two group, B. mandarina and B. mori. B. mandarina has genetic differences according to the population within the Korean peninsula, but that was not recognized genetic variation or divergence considering low values of genetic distance.

Assessment of Genetic Diversity of Horse Breeds Using Microsatellite Makers (Microsatellite makers를 이용한 마품종 간의 평가 및 유전적 다양성)

  • Jung, Ji-Hye;Lee, Mi-Rang;Ha, Tae-Yong;Kim, Seon-Ku;Shin, Teak-Soon;Kang, Han-Seok;Lee, Hong-Gu;Cho, Gil-Jae;Park, Kyung-Do;Cho, Byung-Wook
    • Journal of Life Science
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    • v.19 no.2
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    • pp.169-173
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    • 2009
  • To assist in selection schemes we estimate the genetic diversity of the horse breeds. Genetic diversity at 13 microsatellite loci was compared in six horse breeds : Jeju Native Horse, American Quarter, Jeju Racing Horse, Mongolian Horse, Japanese Horse and Thoroughbred. All of the equine microsatellite used in this study were amplified and were polymorphic. The expected total heterozygosity over all the populations varied between 0.669 and 0.869 and the expected heterozygosity within population range from 0.569 to 0.219 in this study. The low coefficient of gene differentiation value showed that only 0.118 of the diversity was between horses breeds. The constructed dendrogram from the genetic distance matrix showed little differentiation between horse breeds using DISPAN program. The genetic distance using 13 microsatellites ranged between 0.137 and 0.414 for the six horse breeds. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in horse populations. The genetic diversity of the six horse breeds to each other closed to their geographical distribution. Suggesting that the loci would be suitable for horse breeds parentage testing. Therefore, Microsatellite marker seems to be very useful for clarifying the evolutionary relationships of closely related populations.

Genetic diversity and geographic differentiation in the endangered Primula farinosa subsp. modesta, a subalpine endemic to Korea (한반도 아고산대 특산·희귀식물 설앵초의 유전적 다양성과 지리적 분화)

  • Chung, Jae-Min;Son, Sung-Won;Kim, Sang-Yong;Park, Gwang-Woo;Kim, Sung-Shik
    • Korean Journal of Plant Taxonomy
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    • v.43 no.3
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    • pp.236-243
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    • 2013
  • Many plant species in subalpine regions are under threat of extinction as a result of climate change. In this study, the genetic diversity and geographic differentiation of three regions and six populations of Primula farinosa subsp. modesta (Bisset & Moore) Pax in Korea were assessed using the ISSR (Inter Simple Sequence Repeat) marker. The average genetic diversity (P = 60.62, SI = 0.299, h = 0.190) was relatively lower than that of other long-lived perennials, even though it is a self-incompatible species. AMOVA analysis showed that 50% of the total genetic diversity was partitioned among regions and Bayesian cluster analysis showed some remarkable geographic trends that were structured into 2 or 3 regions, suggesting limited gene flow among regions. Considering the population fragmentation, low level genetic diversity, and high genetic differentiation, it is essential to establish in situ and ex situ conservation strategies for P. farinosa subsp. modesta.

Genetic Changes of Cornus controversa with Ozone Exposure (오존 노출에 의한 층층나무의 유전특성 변화)

  • 장석성;이석우;이재천;한심희;김홍은
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.5 no.4
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    • pp.226-232
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    • 2003
  • To examine the effects of ozone (O$_3$), one of the major air pollutants in the city area, on genetic changes in Cornus controversa Hemsl., we compared genetic structures between sensitive (S) and tolerant (T) tree groups of C. controversa fumigated with ozone using isozyme markers. The genetic structures were measured in terms of allele and genotype frequencies determined at ave polymorphic enzyme loci. Marked genetic differences between the two groups were detected at three loci (Lap-2, Mdh-1 and Skdh-1). Genetic parameters, genetic multiplicity, genetic diversity and heterozygosity showed that the tolerant group retained greater genetic variation than did the sensitive group. Results of the study were congruent with the general expectation that the more heterozygous individuals and/or populations exhibit higher resistance to various stress factors.

Genetic Differentiation of Overwintering Populations of Oriental Fruit Moth, Grapholita molesta, and Their Movement (월동세대 복숭아순나방(Grapholita molesta)의 지역적 분화 및 이동)

  • Park, Jung-A;Son, Ye-Rim;Bae, Sung-Woo;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.47 no.3
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    • pp.201-208
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    • 2008
  • Spring phonology of the oriental fruit moth, Grapholita molesta, was monitored using sex pheromone traps in apple cultivating areas. Their occurrence was earlier in southern areas and their population sizes were significantly different among orchards even in a local cultivating zone. The overwintering populations appeared to move between local orchards, based on the fact that monitoring data obtained at the sites between orchards were similar to those of nearby orchards. However, within orchards, these adult movements appeared to decrease and showed skewed occurrences at the side of upwind direction or close to neighboring orchards. At initial occurrence peak(April 20-25), the ovenvintering populations of the different localities were collected and analyzed in their genetic distances. PCR-RAPD analysis indicated that there were significant genetic differences among the overwintering populations of G. molesta. This genetic differentiation of overwinterin populations may be due to genetic bottleneck following differential selection pressures against the subpopulations of G. molesta during winter on the basis of the RAPD analysis that each early spring population was significantly different to its previous fall population in the same locality.

Lack of allozyme variation in the two carnivorous, terrestrial herbs Utricularia bifida and Utricularia caerulea (Lentibulariaceae) co-occurring on wetlands in South Korea: Inference of population history (한반도 남부 지방 습지에 같이 자생하는 식충 육상 초본 2종 땅귀개 및 이삭귀개 (통발과)의 알로자임 변이의 결여: 집단의 역사 추론)

  • Chung, Mi Yoon;Lopez-Pujol, Jordi;Chung, Myong Gi
    • Korean Journal of Plant Taxonomy
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    • v.47 no.4
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    • pp.297-303
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    • 2017
  • In central and southern Korea, the two small insectivorous, terrestrial herbs, Utricularia bifida and U. caerulea, often co-occur at wet locations (or in wetlands). The Korean Peninsula (with central China and northern Japan) constitutes the northern edge of their distribution, as their main range is subtropical and tropical Asia. The Korean populations of both species are very likely of post-glacial origin, given that warm-temperate vegetation was absent from the Korean Peninsula during the Last Glacial Maximum. Two hypotheses of the post-glacial colonization of the peninsula can be formulated; first, if current populations were founded by propagules coming from a single ancestral population (i.e., a single refugium), we would expect low levels of genetic diversity. Alternatively, if contemporary Korean populations originated from multiple sources (multiple refugia), we would expect high levels of genetic variation. To test which is more likely, we surveyed the degree of allozyme variation at 20 loci in ten populations for each of the two species from southern Korea. We found no allozyme variation within each species. However, their aquatic congener U. australis exhibited allozyme polymorphism across Japan (four polymorphic loci at three enzyme systems). We suggest that southern Korean populations of Utricularia bifida and U. caerulea were established by a single introduction event from a genetically depauperate ancestral population.

Speciation of the Two Species of the Genus Hyla (Anura) in Korea (한국산 청개구리속 2조의 종분화에 관한 연구)

  • 양서영;박병상
    • The Korean Journal of Zoology
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    • v.31 no.1
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    • pp.11-20
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    • 1988
  • A comparison between two species of Hyla japonica and H. suweonesis in Korea was carried out to clarify their morphological, genetic and ecological differences, to estimate presumed divergent time between them, and to elucidate their probable process and mechanism of speciation. The results are as follows; 1.The breeding season of H. japonica was earlier than that of H. suweonensis about one and half months. 2.H.japonica was distributed all over the country, while H. suweonensis was restricted in Kyonggi plains. 3.On the extemal morphometric characters, differences were hardiy seen between two species. But on the discriminant function analysis, there was apparent differences between them. 4.Genic variation based on 24 loci showed that H. japonica populations were about two fold more variable than that of H. suweonensis populations. 5.Genetic relatedness between two species was rather remote (S =0.520, D=0.634) 6.Their presumed divergent time was estimated to be about three and a quarter mfllon years. It is presumed that H. suweonensis was originated from H. japonica during the period of late pleocene and early pleistocene. 7.Premating isolating mechanisrns (breeding season, mating call, and microhabitat segregation) may be operating to prevent gene ifow between them.

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The Effects of a Semantic Network Program Instruction for the Learning Achievement and Learning Motivation in High School Biology Class: Centering the Unit of Heredity (동기전략을 적용한 의미망 프로그램 활용 수업이 고등학교 생물 학업성취도와 학습동기에 미치는 효과: 생물I '유전' 단원을 중심으로)

  • Kim, Dong-Ryeul;Moon, Doo-Ho;Son, Yeon-A
    • Journal of The Korean Association For Science Education
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    • v.26 no.3
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    • pp.393-405
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    • 2006
  • The purpose of this study was to analyze the effects of Semantic Network Program (SNP) instruction on learning achievement and motivation in high school biology classes. For this study, a SNP was designed by applying the recommendations in regard to student attention and satisfaction factors in Keller's ARCS theory. SNP instruction was conducted with an experimental group and a control group, each consisting of 62 high school biology class student. A pretest-posttest control group design was employed. The pre-test was used to analyze the learning achievement test, learning motivation test, and semantic forming test. For 4 weeks the experiment group was instructed using the developed SNP which centered on Keller's attention and satisfaction factors, and the control group was instructed via teacher-centered lectures based on the textbook. It was found that SNP instruction efficiently increased students' biology learning achievement (p<.001). It was also discovered that SNP instruction was effective in increasing Keller's motivation strategies on attention and satisfaction factors (p<.001). In addition, SNP instruction positively affected students' semantic formation (p<.001) and learning content retention (p>.05) in the heredity unit by aiding students in the area of active multimedia learning. An in depth interview with students in the class using SNP instruction showed that material learned via this method in biology had longer retention of problem-solving methods. Consequently, SNP instruction according to motivation strategies may high school biology teachers with meaningful teaching-learning methods strategies for the unit on heredity.

Recent Status of Chicken Genome Researches (닭 유전체 연구의 최근 동향)

  • Seo, S.W.;Paek, W.K.;Lee, J.H.
    • Korean Journal of Poultry Science
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    • v.36 no.2
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    • pp.111-115
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    • 2009
  • Chicken has been being used as the protein sources for humans for long times. Since the release of chicken genome sequencing, large efforts have been carried out for identifying valuable genomic information in chicken including the genes affecting quantitative traits, development and immune responses. In this review, the recent progress of genomic researches has been discussed including the available world wide genetic materials in chicken.

Analysis of genetic divergence according to each mitochondrial DNA region of Haliotis discus hannai (북방전복 (Haliotis discus hannai) 의 mitochondrial DNA 영역별 유전적 변이성 분석)

  • Park, Choul-Ji;Nam, Won Sick;Lee, Jeong-Ho;Noh, Jae Koo;Kim, Hyun Chul;Park, Jong Won;Hwang, In Jun;Kim, Sung Yeon
    • The Korean Journal of Malacology
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    • v.29 no.4
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    • pp.335-341
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    • 2013
  • The seven mitochondrial DNA regions (ND2, ND5, ND4, ND4L, ND6, ND1 and 12SrRNA) of Haliotis discus hannai were examined to estimate the availability as a genetic marker for the study of population genetic. The region with the highest genetic variation was ND4 (Haplotype diversity = 1.0000, Nucleotide diversity = 0.0108). On the other hand, ND2 and ND1 regions have significantly appeared genetic divergence between clusters (divergence of 90% and 87%). Also, pairwise $F_{ST}$ between clusters within ND2 and ND1 regions showed high values; 0.4061 (P = 0.0000), 0.4805 (P = 0.0000) respectively. Therefore we can infer that it is the most efficient and accurate way to analyze the region of ND4 with the highest variation in addition to the regions of ND2 and ND1, which formed clusters with high bootstrap value, for study of population genetic structure in this species.