Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Phylogeny of Bombyx mandarina inhabiting Korea analysing the isozyme and hemolymph protein polymorphism

동위효소와 체액단백질 분석에 의한 한국산 멧누에나방의 지역적 특성

  • 이재만 (경북대학교 농업생명과학대학) ;
  • 김경아 (경북대학교 농업생명과학대학) ;
  • 노시갑 (경북대학교 농업생명과학대학)
  • Published : 2003.06.01
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Abstract

B. mandarina of Korean population apparently differs B. mori in isozyme analysis. Fourteen polymorphism occurred B. mandarina not in B. mori at 6 isozymes, Bph, Bes, Amy-hc, Ies, Ict-D, Ict-E. Korean population has shared with the Korean native strain of B. mori in B genotype of Bes, F of Amy-hc, n of Ict-E, M and S of Ict-H. These 5 genotype were known that detection only Korean native strains of B. mori. Nei's genetic distance based on the genotype of isozyme and hemolymph protein using 4 populations of B. mandarina varied from 0.0350 to 0.0624. The distances of 0.0350 is between Jinju and Chilgok population and between Jinju and Kosung population has the largest distances, 0.0624. In genus of Bombyx, B. mandarina and B. mori, genetic distance varied from 0.3822 to 0.5074. Phylogenetic tree obtained using the subprogram UPGMA of NTSYS represented that Bombyx devided two group, B. mandarina and B. mori. B. mandarina has genetic differences according to the population within the Korean peninsula, but that was not recognized genetic variation or divergence considering low values of genetic distance.

한반도에 서식하는 멧누에나방의 서식지역에 따른 유전적 변이와 집누에나방과의 종간 유연관계를 조사하였다. 멧누에나방의 isozyme 및 체액단백질을 대상으로 유전자 빈도를 조사한 결과, 조사지역 중 경북 칠곡과 경남 진주집단 사이의 유전적 유사도가 가장 높았으며, 경남 진주와 강원 고성집단 사이에서 가장 낮게 나타났다. 그러나 서식집단 상호간의 유사도는 매우 높았다. 한국산 멧누에나방과 집누에나방의 isozyme 유전자형은 대부분이 동일하게 발현되었으나, 집누에나방의 한국종에서만 발현된다고 알려진 Bes의 B형, Amy-hc의 F형, Ict-E의 n형 및 Ict-h의 M형과 S형 등의 유전자형이 한반도에 서식하는 멧누에나방에서 발현되었다. 따라서, 한반도 내에서 멧누에나방의 서식지역간 유전적 변이는 인정되지 않으며, 지금까지는 고려되지 않았던 집누에 한국종과 한국산 멧누에나방의 종간 유연관계에 대해 새로운 의문이 야기된다.

Keywords

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