• Journal of the korean academy of Pediatric Dentistry
• /
• v.33 no.4
• /
• pp.615-623
• /
• 2006

The purpose of this study was to evaluate the specificity, sensitivity, and diagnostic power of caries activity test using LED fluorescence. The subjects of this study were 55 children of $6{\sim}7$ years old. LED light were irradiated to labial or buccal surface of all teeth. Fluorescence from initial carious lesion of teeth illuminated by an LED light was observed through barrier filter and the number of teeth showing lesion, size and position of lesion were counted. Streptococcus mutans colony counting and dDfFtT rate test were also done and their correlation was compared. And then specificity, sensitivity, diagnostic power of optical caries activity test using LED light were evaluated. 1. There was positive $correlation({\gamma}=0.43)$ between LED fluorescence test and Streptococcus mutans count(P<0.05). 2. When visual examination was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 76.1%, and 100%. 3. When dDfFtT rate was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 88.9%, 47.8%, and 95.7%. 4. When S. mutans colony counting was defined to standard testing method, the specificity, sensitivity, diagnostic power of LED fluorescence test were 100%, 58.7%, and 100%. Considering the above results, optical caries activity test using LED light could be regarded as a practical method because of its close relationship with microbiological caries activity test.

• Clinical and Experimental Pediatrics
• /
• v.53 no.3
• /
• pp.397-407
• /
• 2010

Purpose : The F9 gene is known to be the causative gene for hemophilia B, but unfortunately the detection rate for restriction fragment length polymorphism-based linkage analysis is only 55.6%. Direct DNA sequencing can detect 98% of mutations, but this alternative procedure is very costly. Here, we conducted multiplex polymerase chain reactions (PCRs) and conformation sensitive gel electrophoresis (CSGE) to perform a screened DNA sequencing for the F9 gene, and we compared the results with direct sequencing in terms of accuracy, cost, simplicity, and time consumption. Methods : A total of 27 unrelated hemophilia B patients were enrolled. Direct DNA sequencing was performed for 27 patients by a separate institute, and multiplex PCR-CSGE screened sequencing was done in our laboratory. Results of the direct DNA sequencing were used as a reference, to which the results of the multiplex PCR-CSGE screened sequencing were compared. For the patients whose mutation was not detected by the 2 methods, multiplex ligation-dependent probe amplification (MLPA) was conducted. Results : With direct sequencing, the mutations could be identified from 26 patients (96.3%), whereas for multiplex PCRCSGE screened sequencing, the mutations could be detected in 23 (85.2%). One patient's mutation was identified by MLPA. A total of 21 different mutations were found among the 27 patients. Conclusion : Multiplex PCR-CSGE screened DNA sequencing detected 88.9% of mutations and reduced costs by 55.7% compared with direct DNA sequencing. However, it was more labor-intensive and time-consuming.

• Tunnel and Underground Space
• /
• v.19 no.1
• /
• pp.19-30
• /
• 2009

For the purpose of determining the thickness of concrete lining and detect of the cavity where may be located behind tunnel lining, IE (Impact-Echo) method it effectively useful in the tunnel safety diagnosis and the quality control during the construction. As a part of case study, we applied IE method to various tunnel structure types such as road tunnel and subway tunnel constructed by NATM (New Austrian Tunnelling Method) and ASSM (American Steel Support Method). As tunnel specifications estimated from this method were compared with coring data, design drawing and other survey results, it was very good agreement with each other. In conclusion, we verified that IE method shows an accurate and reliable result. The conventional interpretation of IE method in frequency domain gives only vertical information at a certain point. However, the interpretation using time-frequency analysis and depth section imaging technique from two dimensional profiling surveys can show more reliable information about structure inside.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
• /
• v.12 no.1 s.100
• /
• pp.81-88
• /
• 1991

토양전염성 병을 효과적으로 방제하기 위해서는 병의 진단에서 부터 전염원 동태 파악, 발병환경구명등 기본적인 연구자료의 축적위에서 현행 방제법들을 적절히 조화시킨 종합방제 체제를 확립하여야 한다.

• 전기의세계
• /
• v.45 no.4
• /
• pp.40-44
• /
• 1996

본고에서는 전력케이블의 고체절연재료의 열화기구, 사고 케이블절연재료의 분석 결과와 열화진단법에 대하여 소개하고자 한다.

• Magazine of the Korea Institute for Structural Maintenance and Inspection
• /
• v.16 no.1
• /
• pp.4-11
• /
• 2012

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2000.07a
• /
• pp.269-274
• /
• 2000

• Proceedings of the Korean Vacuum Society Conference
• /
• 2008.08a
• /
• pp.307-307
• /
• 2008

• Parasites, Hosts and Diseases
• /
• v.22 no.2
• /
• pp.223-228
• /
• 1984

Seven cases of surgically proven sparganosis were serologically tested by means of microELISA for their specific IgG antibody levels. For that purpose, crude saline extract of spargana from snake, Natrix tigrina lateralis was prepared and used as antigen. The sparganosis sera were also tested with Paragonimus and Cysticercus antigens to observe the cross reactivity. A total of 71 sera from normal control, ectopic and pulmonary paragonimiasis, clonorchiasis, cysticerCOSIS and Taenia saginata cases were also included. Except for one case of old calcified infection, all of 6 human sparganosis showed higher serum levels of specific IgG antibody when the differential point of positive reaction was set at the absorbance value of 0.25 (the sensitivity being 85.7%). In control and other helminthic infections, all except 3 cases of T. saginata infection showed negative reaction to sparganum antigen (the specificity being 90.7%). None of sparganosis cases showed cress reactivity to Paragonimus and Cysticercus antigens. Undiluted cerebrospinal l1uid also showed high levels of antibody when central nervous system was invaded. The serologic diagnosis by means of micro ELISA could be a useful tool in epidemiological study of human sparganosis in susceptible population, as well as in individual diagnosis.

• Applied Chemistry for Engineering
• /
• v.31 no.6
• /
• pp.585-590
• /
• 2020

Successful early diagnosis of cancer diseases such as lung, prostate, liver and adrenocortical carcinoma is a key step in determining the cost of treatment, survival rate, and cure rate. Most of current cancer diagnosis systems including biopsy, computed tomography (CT), positron emission tomography (PET)-CT, magnetic resonance imaging (MRI), ultrasonography, etc., require expensive and complicated equipment with highly trained human resources. Global medical and scientific communities have thus made numerous efforts on developing effective but rapid disease management system via introducing a wide spectrum of point-of-care medical diagnosis devices. Among them, a lateral flow immunoassay strip technique has gained a great attention due to many advantages such as cost-effectiveness, short inspection time, and user friendly accessibility. In this mini-review, we will highlight recent research trend on the development of colorimetry based LFIA strips for cancer diagnosis and discuss the future research direction and potential applications.