• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.155-164
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• 2004

This involves identifying and cloning trapped genes from cultured cells carrying the gene-trap constructs and generating cloned zebrafish using these cells for functional study. Gene-trapping studies in gene-trapped cells were carried out in initial and cloned zebrafish carrying gene-trap events were successfully produced based on the nuclear transplantation technique. Two kind of retroviral gene-trap constructs were adopted. The first one(SA/GFP-TP), constructed in my laboratory, carries a GFP reporter gene containing a splicing acceptor and an internal neo gene. The second one(Neo-TP), obtained from Dr. Hicks (Hicks et al., 1997), contains a promoter-less neo gene located in the LTR sequence of a retroviral vector. The infected cells were subjected to drug selection(neomycin treatment) because the two constructs carry the neomycin resistant gene. All those cells survived the neomycin treatment should carry the proviral insertions. For Neo-TP, Isolated DNA from the neomycin-resistant fibroblast cells infected by Neo-TP, was digested with EcoR1 restriction enzyme and transformed into bacteria after ligation. This procedure led to the isolation of seven clones carrying flanking cellular DNA with a typical retroviral integration signature sequence. These clones contained genomic DNA ranging from 1kb to 7kb and sequences of 300-600 bp were obtained from each of the rescued plasmids. Database searching showed that all of them share high homology to zebrafish sequences. For fish cloning using tagged cells, initially, nucleus donors directly selected from a mixture of cells(Neo-TP cells) were used. A total of 44 embryos(3.7%) out of 1179 transplants were reached blastula stage; 8 of these embryos(0.8%) hatched and 3(0.3%) of them survived to adulthood. One out of three lived cloned zebrafish has an amplified fragment and was labeled with 32P.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2010.11a
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• pp.239-241
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• 2010

분산 비디오 기술의 대표적인 기술 중 하나는 와이너 지브 부호화 기술이다. 와이너 지브 부호화 구조에서 보조정보는 인트라 복호화된 키 픽처들을 이용하여 생성한다. 키 픽처의 객관적 화질은 보조정보의 성능에 많은 영향을 끼치고, 잡음이 많은 보조정보를 복호화에 이용할 경우 부호화로부터 많은 패리티 비트를 요구하게 되어 율 왜곡 성능을 저하된다. 기존의 부호화 기술은 키 픽처 부호화 시 Quantization Matrix에 따라 미리 정의된 양자화 계수를 이용한다. 본 논문에서는 미리 정의된 양자화 계수 보다 낮은 계수 값을 사용하여 부호화 하는 방법을 제안한다. 제안방법은 키 픽처의 객관적 화질이 높아짐에 따라 보조정보의 화질을 향상시킨다. 잡음이 적은 보조정보는 와이너 지브 복호화 시 율 왜곡 성능을 향상시킨다. 실험결과는 기존 방법에 비해 최대 0.7dB에 이르는 성능향상을 보인다.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2011.07a
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• pp.595-597
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• 2011

분산 비디오 기술의 대표적인 기술 중 하나는 와이너 지브 부호화 기술이다. 종래 와이너 지브 부호화 기술에서는 낮은 복잡도의 부호화기를 얻기 위하여 일반적으로 인트라 부호화된 키 픽처를 사용한다. 하지만 인트라 부호화 방법은 공간적 중복성분만을 제거하기 때문에, 정적인 영상에서 부호화 효율이 매우 떨어지게 되어, 전체적인 율 왜곡 성능을 저하시킨다. 본 논문에서는 움직임 벡터를 (0, 0)으로 간주한 제로 모션 키 픽처를 이용하여 공간적 중복성분 이외에 시간적 중복성분 또한 제거하여 부호화 효율 성향을 위한 방법에 대한 종래 인트라 키 픽처 대비 율 왜곡 성능을 비교하여 보이고, 제로 모션 키 픽처 부호화의 복잡도가 기존 인트라 부호화 방법과 유사한 것을 보였다. 실험결과 기존 방법에 비해 유사한 복잡도를 갖으면서, 정적인 영상에서 최대 56%의 비트율 이득을 얻었다.

• Proceedings of the Korean Institute of Industrial Safety Conference
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• 2000.11a
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• pp.411-416
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• 2000

타워크레인은 빌딩, 아파트, 교하공간이 높은 교량, 플랜트 공사 등 고소작업이 이루어지는 공사에서 유익하게 사용되는·건설장비이다 타워크레인은 단면에 비하여 그 설치높이가 높아서 매우 세장한 구조물이며, 마스트 상부에 설치되어 있는 상부구조물(타워 해드, 지브, 카운터 지브, 카운터 웨이트, 기계장치 등)의 자중이 매우 크기 때문에 하중균형, 풍하중, 인양하중 등에 아주 민감한 강구조물이다. 따라서 이러한 타워크레인의 특성에 대한 이해의 부족과 안전한 작업방법의 불이행 등으로 인한 사고가 가끔 발생되고 있으며, 타워크레인의 사고는 곧바로 중대재해로 이어지고 있다.(중략)

• Proceeding of EDISON Challenge
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• 2014.03a
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• pp.643-648
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• 2014

세일링 요트 (sailing yacht)는 세일 (sail)의 양력을 주로 이용하여 추진력을 얻는다. 요트 경주에서와 같이 항주 속도가 중요한 문제에서는 세일의 성능이 좋을수록 빠른 속도를 낼 수 있기 때문에 세일 주변의 유동해석을 통한 성능 추정과 효율의 최적화는 매우 중요하다. 본 논문에서는 일반적인 경주요트의 형태로써 지브 세일 (jib sail)과 메인 세일 (main sail) 그리고 한 개의 마스트(mast)로 구성된 슬루프(sloop)형 요트에 대한 유동해석을 하였다. 풍상범주 상태에서의 30ft급 세일링 요트인 KORDY-30의 jib sail과 main sail의 높이에 따른 단면을 모델링하였다. 슬루프형 요트의 경우, 세일링 요트에서 jib sail과 main sail이 각각 단독으로 있는 형태의 요트보다 세일의 개수가 증가함에 따라 양력이 증가하지만 항력도 따라서 증가 할 것이라고 가정했다. 따라서 jib sail의 각도를 다양하게 변화시켜 양력계수와, 항력계수의 변화를 살펴보고 그에 따른 양항비의 분석을 통해 최적의 효율을 갖는 jib sail의 각도를 알아보았다.

• The Journal of the Korea Contents Association
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• v.16 no.6
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• pp.9-18
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• 2016

This paper proposes a multi-view Wyner-Ziv Video coding scheme based on spatio-temporal adaptive estimation. The proposed algorithm is designed to search for a better estimated block with joint bi-directional motion estimation by introducing weights between temporal and spatial directions, and by classifying effectively the region of interest blocks, which is based on the edge detection and the synthesis, and by selecting the reference estimation block from the effective motion vector analysis. The proposed algorithm exploits the information of a single frame viewpoint and adjacent frame viewpoints, simultaneously and then generates adaptively side information in a variety of closure, and reflection regions to have a better performance. Through several simulations with multi-view video sequences, it is shown that the proposed algorithm performs visual quality improvement as well as bit-rate reduction, compared to the conventional methods.

• Cartoon and Animation Studies
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• s.49
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• pp.221-246
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• 2017

Visual narrative or storytelling involve two important aspects of the cognitive perception: the conscious and subconscious. Color is one of the cognitive storytelling elements, its interpretation is captured by the subconscious and it is considered an emotional resource due to its psychological background. On the other hand, the character is also part of cognitive perception and storytelling tool, but this is interpreted consciously, character is considered as a logical resource. Ghibli and Disney have organized their cinematographic techniques in different ways, so it would be unfair to say that one narrative style is better than the other, it depends of perception of beauty which varies according to society. However the present study seeks to determine the differences between Ghibli and Disney narrative style, taking as reference color. Ghibli and Disney are perfect example about color effects as a storytelling agent, It can be classified in two aspects: dynamics of color and color as a narrative sequence. The dynamics of color analyzes how color acts and is perceived into a stage, its meaning and symbolism regardless of the character's actions. The narrative sequence studies how color thermologic evolves in scene's development.

• Cartoon and Animation Studies
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• s.43
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• pp.185-209
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• 2016

Pattern refers to singular or repetitive decorative form in a blank surface, Also pattern is not just something to simply fill in the blanks, and has more meanings. Pattern reflects the specific culture or regional feature. So Depending on which pattern to use, creator can give particular of identity. which is generally utilized in visual arts such as painting, architecture, craft, as well as animation. Pattern in animation plays a role of decorating background or surfaces of characters' outfits or props. And Parttern is effective way to describe the story of the times and space environment of the background. Tomm Moore, an animation director in in Ireland mainly produces animations based on traditional folk stories or myths. He usually utilizes cultural and artistic factors related to the themes in his work production. One example is the insertion of pattern closely associated with the narratives and backgrounds of animations to create profound scenes. Tomm Moore used the Irish Celt pattern in Secret of Kells (2009) and Song of Sea (2014) and Islam geometric pattern and plant pattern in a short nimation named On Love from Kahlil Gibran's The Prophe (2014). This study attempts to examine the historical and cultural foundation and the narratives of these two animations, Song of Sea(2014) and Kahlil Gibran's The Prophet: On Love (2014) in which Tomm Moore participated as the director and producer, exploring their relevant traditional patterns. Moreover, it also attempts to analyze how these traditional patterns are utilized in the animations.

• Journal of Digital Convergence
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• v.19 no.9
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• pp.289-297
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• 2021

Animation is a media reflecting social ideas, not only conveying information but also creating a new discourse. The purpose of this paper is to examine the social issues and gender discourse reflected in the media by analyzing the gender discourse inherent in the protagonist and surrounding characters, narrative characteristics, and background of the Ghibli animation "The Tale of the Princess Kaguya". As a research method for this purpose, figure out social problems related to gender performance, and analyze prior researches to derive a connection with the gender discourse in the animation. Grandfather is symbolic of a father who forces his daughter to follow his chosen happiness, and grandmother represents a mother who can not stop her husband. Stemaru is the character not in the original, posing a question of the problem of gender performance while failing to get married due to lack of sufficient wealth and honor. Princess Kaguya had been self-reliant and active like a modern woman at the beginning of the story. However, after being suppressed by society and people around her, the stereotyped gender performance forced Kaguya, who longed for the earth, to wish to return to the moon. Such social pressure happens in modern society. Gender performance is a systemic problem to be solved with systemic change. For this reason, constant consideration such as this paper is needed.

• Korean Journal of Construction Engineering and Management
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• v.15 no.4
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• pp.95-106
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• 2014

The outer surfaces of free form buildings contain panels with two-directional curvatures. To construct these panels, complex geometric surfaces should be divided into forms and sizes that can be manufactured and constructed efficiently. Because the bigger the curvatures of these panel, the more expensive the construction costs, these complex curvatures should go through optimal process of reinterpretation to minimize the curved surfaces with complex two-directional curvatures, which is called panel optimization. Small construction and design companies have trouble in calculating even rough estimate and cannot adjust expected construction cost of the panels based on comparison of design alternatives in conjunction with panel optimization process due to lack of knowledge and experience. This study conducts the research that can support designers' cost decision-making in the design stage of the free form buildings with respect to the panel optimization process. A 3D commercial application specialized to modeling free form shapes is used for the purpose.