• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.4
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• pp.1770-1774
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• 2011

This paper was to characterize the transfer of diacylglycerol(DAG) from lipophorin to Manduca sexta larval fat bodies. $[^3H]$-DAG-labeled Lp($[^3H]$-DAG-Lp) was incubated with the larval fat bodies under different times and the time of DAG transfer was determined. Incubation of fat bodies with $[^3H]$-DAG-Lp resulted in accumulation of DAG and TAG in the tissue. The transfer of $[^3H]$-DAG was inhibited in the presence of suramin and unlabeled lipophorin, which would be consistent with a lipophorin receptor. The effects of suramin may be complex because it can change membrane properties when bound to the lipophorin receptor and affect the rate of DAG transfer. To investigate the lipid uptake via receptor-mediated endocytosis, we treated with endocytosis inhibitors, ammonium chloride and chloroquine. The results show that the transfer process of lipid by lipophorin and fat bodies is receptor-mediated endocytosis.

• The Korean Journal of Zoology
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• v.38 no.1
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• pp.106-114
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• 1995

배추흰나비의 조직과 발생시기에 따른 발생 특이 큐티클단백질의 질적 분포를 조사하기 위해 전기영동법, western blotting 및 autoradiography 법을 사용하였다. SDS-PAGE에서 유충기에는 4개, 용기에는 3개의 단백질이 발생 특이 큐티클단백질로 확인되었다. 전기영동적 이동도로 보아 유충기의 62 Kd 큐티클단백질은 지방체 표피, 혈림프에 모두 분포하며. 22 Kd, 16 Kd 단백질은 지방체와 표피, 그리고 19 Kd 단백질은 지방체에서 확인되고 있으며, 용기의 37 Kd 단백질은 표피에서. 28 Kd, 27 Kd 단백질은 표피와 지방체에 분포하는 양상을 보이고 있다 면역학적 방법으로 발생 특이 큐티클단백질의 동질성을 조사한 결과 용기의 27 Kd 단백질은 표피와 지방체에서 반응을 나타내며, 유충기의 22 Kd 단백질은 단지 표피에서만 미약하게 이의 동질성을 보여주고 있다 즉, 27 Kd 단백질은 지방체 22 Kd 단백질은 표피에서 기원하고 있어 발생 특이 큐티클단백질의 합성부위는 한 조직에서 기원하는 것이 아니라 각기 상이한 조직으로 부터 생성되는 것으로 사료된다.

• Korean Journal of Environment and Ecology
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• v.28 no.5
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• pp.495-499
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• 2014

To search for myrmecochorous plant species, related ant species and diaspore selecting behaviour, collecting fruits and diaspores of four Corydalis species, cafeteria experiments, and the related ant species were studied from May 2013 to July 2014. Four Corydalis species recording in Korea were proved myrmecochorous (seed attached elaiosome) plants newly. Elaiosome shape of these species is spatulate-shaped, but their size is varied considerably in length and width. Significant differences is proved between four plant species in weight of diaspores, seeds and elaiosomes, and elaiosome ratios. Mean values of diaspores weight showed 4.08 mg, 2.56 mg, 2.49 mg and 2.06 mg in Corydalis grandicalyx, C. remota, C. turtschaninovii for. pectinata and C. turtschaninovii var. linearis, respectively. Mean values of seed weights showed 3.68 mg, 2.03 mg, 1.90 mg and 1.67 mg in Corydalis grandicalyx, C. remota, C. turtschaninovii for. pectinata and C. turtschaninovii var. linearis, respectively. Elaiosome ratio of C. turtschaninovii var. linearis was the highest, followed by C. remota, C. turtschaninovii for. pectinata and C. grandicalyx. Ant's preference for the diaspores is ordered as same as elaiosome ratio's order. Major ant species transporting the diaspores of Corydalis species are Formica japonica, Lasius alienus, Lasius japonicus and Tetramorium tsushimae. Formica japonica transports the diaspores most frequently. Ant's preference for the diaspores among plant species or individuals might be influenced on the survival rates of concerned species. Further studies on the myrmecochorous plant and related ant species, cafeteria experiments should be needed.

• Korean Journal of Environment and Ecology
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• v.31 no.6
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• pp.485-491
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• 2017

This study examined the germination characteristics by collecting fruits and pretreatment of the diaspore from April 2014 to June 2015 to investigate the diaspore characteristics, seed dispersion, and seed germination characteristics of two myrmecochorous spring ephemerals (MCSE): -Jeffersonia dubia (Jb) and Corydalis remota (Cr)-. The diaspore of Jb was about 5mm-long yellow-dark brown, oblong seeds with the attached amorphous white elaiosome. The mean weights of diaspore, seed, and elaiosome were 15.86mg, 13.46mg, and 2.40mg, respectively, and the elaiosome ratio was 15.13%. The diaspore of Cr was about 1.2mm diameter and glossy black ovoid seeds with the attached white spatula-shaped elaiosome. The mean weights of diaspore, seed, and elaisome were 2.58mg, 2.05mg, and 0.53mg, respectively, and the elaiosome ratio was 20.54%. Camponotus niponensis and Formica japonica transported the diaspore of Jb while Formica japonica and Lasius japonicus transported the diaspore of Cr. The germination percentage of Jb seeds was statistically significant and had the significance level of 1% with the pretreatment and date of sowing. However, it was independent of attachment of elaiosome. The mean germination percentages of Jb seeds was 65.0% during sowing on June 20, 17.5% during sowing on August 19, and 0% during sowing on October 20. The germination percentage of Cr was statistically significant and had the significance level of 5% and 1% with the attachment of elaisome and date of sowing, respectively. The mean germination rates were 54.17% and 35.0% in the non-treatment section and the treatment section with elaisome detached, respectively. The mean germination percentages of Cr seeds was 75.0% during sowing on June 20, 53.75% during sowing on August 19, and 5.0% during sowing on October 20. Considering the fact that the ants transported the diaspores to the ant house when the fruits of MCSE were ripened and dropped the seeds, the direct seeding right after collecting may be most suitable to the characteristics of the evolution of these plant species and may be the best method to obtain the highest germination percentages. Since the ants distribute their seeds, the MCSE produces and attaches the elaiosome to the seed to maintain the symbiotic relationship with ants. The ants then transport the seed to the ant house where the environment is controlled for suitable temperature and humidity, and then the MCSE succeeds in germination after the embryo grows sufficiently in the next spring.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.8
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• pp.4106-4110
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• 2013

Apolipophorin-III (apoLp-III) was isolated and purified from the last larval hemolymph of Galleria mellonella by the KBr gradient ultracentrifugation and gel chromatography (Sephadex G-100). In this paper, we examined that apoLp-III is taken up into the last larval fat bodies in Galleria mellonella. The last larval fat body tissues were incubated at room temperature for 30 min with fluorescein isothiocyanate (FITC)-labeled apoLp-III (FITC-apoLp-III). Fluorescein microscopy and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) revealed that the last larval fat body tissues internalize FITC-apoLp-III. The results show that the apoLp-III is taken up by the last larval fat body.

• Proceedings of the KAIS Fall Conference
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• 2010.11b
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• pp.950-953
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• 2010

지방자치단체는 재정 확충 및 건전도 유지에 많은 노력을 기울이고 있다. 그러한 노력 중의 하나는 기업체 유치와 주민유치이다. 이는 기업체 유치가 법인세 증가로 지방재정에 기여하게 되며, 주민의 수 역시 주민세와 같은 직접적 효과와 함께 간접적 효과가 발생할 것으로 보기 때문이다. 본 연구는 충북지방을 대상으로 각 기초자치단체 경영에 있어서 이러한 기업체 유치 노력과 주민 확보 노력이 지자체 재정에 어떠한 영향을 미치는지를 분석하기 위한 기반 연구로서 먼저 재정에 미칠 가능성이 있는 각 변수이 상관관계를 분석하고 이에 근거하여 구조방정식 전개를 위한 개념적 모형을 제안하였다.

• The Korean Journal of Zoology
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• v.36 no.3
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• pp.416-424
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• 1993

Ovarian Yolk protein (YP2) synthesis has been investigated in mosquito, Culex pipiens pallens. Yolk protein amount which was syntheized in fat body, accumulated into ovary were analyzed by Rocket immunoelectrophoresis and in vitro organ culture. The result was that yolk protein synthesis began to occur at 6hrs after blood meal, reached at maximum level by 24hrs, and was completed within 48hrs. Yolk protein accmulation into the ovary began to start at 6hrs and coutinued for up to 60hrs after blood meal. Extract from 0, 24, 48, 72hrs ovaries after blood meal were analyzed by electrophoresis and Western blotting. The result was that 24hrs ovary contain one yolk protein(YP1), and 48, 72hrs ovaries contain two kinds of yolk proteins(YPl and YP2). When 48hr ovaries and fat bodies were incubated in $^3$H-leucine contained medium, protein synthesis was not occurred in fat body, but ovary synthesized much protein contained yolk protein (YP2). The result of crossed immunoelectrophoresis represented the same immunity between YPl and YP2. The present data suggest that ovary synthesize yolk protein(YP2) in mosquito, Culex pipiens pallens.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.182.1-182
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• 1994

• Journal of Life Science
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• v.27 no.2
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• pp.233-240
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• 2017

Previously, we have shown that Schisandra chinensis (Turcz.) Baill. (S. chinensis) has a protective effect against endoplasmic reticulum (ER) stress-induced hepatic steatosis. Gomisin A is a bioactive phytoestrogen derived from S. chinensis. In the present study, the in vitro and in vivo effects of gomisin A on ER stress and hepatic steatosis were investigated. We quantified the expression of markers of ER stress, including glucose regulated protein 78 (GRP78), C/EBP homolog protein (CHOP), and X-box-binding protein-1 (XBP-1), in HepG2 cells treated with tunicamycin or palmitate. Tunicamycin treatment in HepG2 cells induced the expression of markers of ER stress, including GRP78, CHOP, and XBP-1c. However, treatment with gomisin A reduced the expression of markers of ER stress. These inhibitory effects were also observed in palmitate-incubated HepG2 cells. The in vivo inhibitory effects of gomisin A were assessed in mice injected with tunicamycin or fed with a high fat diet (HFD). Gomisin A reduced the expression of markers of ER stress and decreased triglyceride levels in the livers of mice after tunicamycin injection or HFD feeding. Furthermore, gomisin A decreased the expression of inflammatory genes in palmitate-incubated HepG2 cells and the liver of HFD-fed obese mice. These results suggest that gomisin A inhibits ER stress and ameliorates hepatic steatosis induced by ER stress.

• Review of KIISC
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• v.22 no.7
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• pp.27-36
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• 2012

일본은 보이는 행정 서비스, 열린 정부, 행정 쇄신을 위하여 전자행정 클라우드를 추진하고 있다. 이러한 일본의 전자행정 클라우드는 크게 정부의 전자행정 클라우드인 카스미가세키 클라우드와 지방자치체의 전자행정 클라우드인 자치체 클라우드로 나누어서 추진되고 있다. 본 논문에서는 일본 자치체 클라우드를 중심으로 자치체 클라우드 도입 추진 현황, 자치체 클라우드 추진 전략, 자치체 클라우드 도입 효과 등에 대하여 살펴보고, 우리나라 지방 자치 단체 클라우드 도입을 위한 사전 방안 검토를 제시하였다.