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http://dx.doi.org/10.5762/KAIS.2011.12.4.1770

The transfer of diacylglycerol from lipophor in to fat body in larval Manduca sexta  

Yun, Hwa-Kyung (Department of Biological Sciences, Hanseo University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.12, no.4, 2011 , pp. 1770-1774 More about this Journal
Abstract
This paper was to characterize the transfer of diacylglycerol(DAG) from lipophorin to Manduca sexta larval fat bodies. $[^3H]$-DAG-labeled Lp($[^3H]$-DAG-Lp) was incubated with the larval fat bodies under different times and the time of DAG transfer was determined. Incubation of fat bodies with $[^3H]$-DAG-Lp resulted in accumulation of DAG and TAG in the tissue. The transfer of $[^3H]$-DAG was inhibited in the presence of suramin and unlabeled lipophorin, which would be consistent with a lipophorin receptor. The effects of suramin may be complex because it can change membrane properties when bound to the lipophorin receptor and affect the rate of DAG transfer. To investigate the lipid uptake via receptor-mediated endocytosis, we treated with endocytosis inhibitors, ammonium chloride and chloroquine. The results show that the transfer process of lipid by lipophorin and fat bodies is receptor-mediated endocytosis.
Keywords
Lipophorin; Fat bodies; Endocytosis; Diacylglycerol;
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Times Cited By KSCI : 2  (Citation Analysis)
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