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http://dx.doi.org/10.5762/KAIS.2013.14.8.4106

Apolipophorin-III uptake by the last larval fat body in the wax moth Galleria mellonella  

Yun, Hwa-Kyung (Department of Biological Sciences, Hanseo University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.14, no.8, 2013 , pp. 4106-4110 More about this Journal
Abstract
Apolipophorin-III (apoLp-III) was isolated and purified from the last larval hemolymph of Galleria mellonella by the KBr gradient ultracentrifugation and gel chromatography (Sephadex G-100). In this paper, we examined that apoLp-III is taken up into the last larval fat bodies in Galleria mellonella. The last larval fat body tissues were incubated at room temperature for 30 min with fluorescein isothiocyanate (FITC)-labeled apoLp-III (FITC-apoLp-III). Fluorescein microscopy and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) revealed that the last larval fat body tissues internalize FITC-apoLp-III. The results show that the apoLp-III is taken up by the last larval fat body.
Keywords
Apolipophorin-III; Fat bodies; FITC; SDS-PAGE;
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Times Cited By KSCI : 2  (Citation Analysis)
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