• Journal of Nutrition and Health
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• v.47 no.1
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• pp.1-11
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• 2014

Purpose: Several studies have proven that EGCG, the primary green tea catechin, and glucosamine-6-phosphate (PGlc) reduce triglyceride contents in 3T3-L1 adipocytes. The objective of this study is to evaluate the combination effect of EGCG and PGlc on decline of accumulated fat in differentiated 3T3-L1 adipocytes. Methods: EGCG and PGlc were administered for 6 day for differentiation of 3T3-L1 adipocytes. Cell viability was measured using the CCK assay kit. In addition, TG accumulation in culture 3T3-L1 adipocytes was investigated by Oil Red O staining. We examined the expres-sion level of several genes and proteins associated with adipogenesis and lipolysis using real-time RT-PCR and Western blot analysis. A flow cytometer Calibar was used to assess the effect of EGCG and PGluco on cell-cycle progression of differentiating 3T3-L1 cells. Results: Intracelluar lipid accumulation was significantly decreased by combination treatment with EGCG $60{\mu}M$ and PGlc $200{\mu}g/m$ compared with control and EGCG treatment alone. In addition, use of combination treatment resulted in directly decreased expression of $PPAR{\gamma}$, $C/EBP{\alpha}$, and SREBP1. In addition, it inhibited adipocyte differentiation and adipogenesis through downstream regulation of adipogenic target genes such as FAS, ACSL1, and LPL, and the inhibitory action of EGCG and PGlc was found to inhibit the mitotic clonal expansion (MCE) process as evidenced by impaired cell cycle entry into S phase and the S to G2/M phase transition of confluent cells and levels of cell cycle regulating proteins such as cyclin A and CDK2. Conclusion: Combination treatment of EGCG and PGlc inhibited adipocyte differentiation through decreased expression of genes related to adipogenesis and adipogenic and cell cycle arrest in early stage of adipocyte differentiation.

• Proceedings of the Korean System Dynamics Society
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• 2006.04a
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• pp.123-149
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• 2006

본 연구는 방사성폐기물 처분시설(radioactive waste repository)의 입지를 가정하여, 처분시설이 경상북도 경주시에 발생시키는 경제적, 사회적 효과를 분석하는데 목적이 있다. 정부는 처분장 유치의 유인책(incentives)으로서 경주 지역주민들을 위하여 다양한 정책적 수단을 마련하였다. 처분시설 입지에 따른 특별지원금 3,000 억원 지원, 수거물 반입 수수료 지원(년당 50-100억원), 한국수력원자력(주) 본사이전, 양성자가속기 사업 추진 등이 그것이다. 이들 지원사업들이 가져오는 변화를 시스템 다이내믹스(System Dynamics) 기법을 적용하여 지역사회의 인구, 산업, 토지, SOC, 지방재정 등이 어떻게 변화하는지 추적해 봄으로써 도시체제의 동태성(urban system dynamics)을 이해하고 처분장 시설이 지역에 입지했을때, 미래에 발생 가능한 문제점이 없는지 밝혀내고자한다. 이를 위하여 시뮬레이션 모델링에 입지 지역의 특성과 현황을 반영하여 처분장입지에 따른 지역의 동태적인 변화과정과 경향을 추정해 보고, 현재 예정되어 있는 지원사업이 충분한지, 이외에 다른 정책적 지원이 필요한지를 알아본다. 본 연구의 의미는 이처럼 경주지역 주민들이 처분장의 지역입지를 만족스럽게 행각하고 소외감 없이 생활을 영위할 수 있도록 정책적 지원 프로그램을 작성하는데 기초가 되는 연구라는 점에 있다고 하겠다.

• Social Workers
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• no.4 s.36
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• pp.4-13
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• 2005

2004년 12월 17일, 정부혁신위원회는 가족정책을 기존 보건복지부에서 여성부로 이관 할 계획이라고 밝혔는데, 지난 3월 2일 국회 본회의에서 이와 관련된 '정부 조직법'이 통과됨으로써 오는 5월부터 본격적으로 가동되게 된다. 이는 여성부가 여성가족부로 확대, 개편됨에 따라 앞으로 가족정책을 모두 총괄하는 기능을 갖는 다는 의미인데, 작년 사회복지제도가 격렬하게 반대의견을 내건 '건강가정기본법'과 '모.부자복지법이 이관된다. 이 같은 변화는 지난 2001년 여성의 권익증진, 차별개선 등 여성정책 시행을 위해 여성부가 출범하여 지난해 '영유아보육 업무'를 이관 받은 뒤 5년도 체 안되어 가족업무까지 맡겨져 여성부의 어깨를 한층 더 무겁게 하는 것이다. 정부혁신지방분권위는 작년 말 이 같은 계획을 발표하여 전통적 가족구조가 무너져 가족해체 현상이 크게 나타나며, 이에 대한 국가차원의 종합적인 예방정책의 1단계로 이같이 조정했다고 밝혔다. 그리고 이전부터 여성청소년가족부를 만들어 통합관리하자는 주장을 제기해 온 여성부도 이번 변화에 대해 굳은 의지를 보였다.

• Environmental and Resource Economics Review
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• v.10 no.4
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• pp.483-494
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• 2001

본 연구는 항공기 소음이 주택가격에 미치는 영향을 헤도닉가격기법을 적용하여 분석하고자 하였다. 국제공항이 인천으로 이전하기 전 서울지방항공청장이 소음피해지역(제1종, 제2종 및 제3종)으로 고시한 김포공항 주변의 아파트 가격을 주요 분석 대상으로 하였다. 소음측정단위로는 세계민간항공기구가 권장하는 연속적 동등소음감지단위(Weight Equivalent Continuous Perceived Noise Level: WECPNL)를 사용하였다. 2000년도 여름에 매매된 총 220가구를 대상으로 아파트의 실질거래가격과 소음측정단위, 가구속성 및 지역관련 변수를 사용하여 헤도닉 방정식을 추정한 결과 소음변수를 비롯 아파트 평수 및 방의 개수를 나타내는 변수에 대한 계수의 추정치는 통계적으로 매우 유의적으로 나타났다. 분석대상지역에 있어 항공기 소음의 한계내포가격은 평균 마이너스 340여 만 원으로 추정되었다. 이 추정치는 다시 말해 소음감지단위인 WECPNL이 한 단위 증가하게 되면 아파트의 평균매매 가격의 3.19%가 떨어지는 것을 의미하며 이 지역이 소음피해지역으로 고시되어 있는 상황을 매우 잘 나타내고 있었다.

• The Korean Journal of Zoology
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• v.37 no.2
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• pp.267-273
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• 1994

빨간집모기가 흡혈한 후 체내에서 일어나는 total RNA 변화를 조사한 결과 흠혈 후 6시 간 이후에 RNA양이 증가하기 시작하여 18시간에서 peak를 보인 후 감소하다가 30시간째 부터 증가하기 시작하여 48시간 후에 최대의 RNA 양을 보인 후 급격히 감소하였다 난소에서의 RNA 합성을 정량한 결과 흡혈 후 24시간 이전에는 흡혈 전에 비하여 전혀 증가하지 않았고 30시간 이후부터 증가하기 시작하여 48시간에 가장 많은 양의 RNA가 난소내에 존재하였고, 그 후 급격히 감소하였다. 흡혈 후 6시간 간격으로 난소로 부터 total RNA를 추출하여 in vitro translation을 실시하고 TCA 침전법과 면역침전법으로 합성된 3H-protein과 3H-vitellogenin을 정량하였다. 그 결과 흡혈 후 36시간 이후의 난소로부터 3H-protein과 지-vitellogenin의 합성이 일어나기 시작하여 48시간된 난소에서 가장 많은 양의 3H-protein과 3H-vitellogenin이 합성되었으며, 이때 합성된 단백질의 약 45% 정도가 난황단백질인 3H-vitellogenin으로 나타났다 이상의 결과로 빨간집모기에서는 지방체에서 뿐만 아니라 난소에서도 난황단백질의 합성이 일어남을 알수 있다.

• SUVANNABHUMI
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• v.1 no.1
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• pp.91-109
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• 2009

오늘날 태국 남부 국경 부근의 3개주와 말레이 북부의 일부가 포함된 지역은 불교 사회인 태국과 무슬림 사회인 말레이라는 문화적인 차이가 있음에도 불구하고 그 문화적 근원을 찾아 보면 분명히 이전의 타이불교와 말레이 무슬림의 근원을 만나게 된다. 이는 역사적으로나 고고학적으로나 혹은 지방문학에서도 뿌리를 찾을 수 있는데 특별히 이 논문에서는 땀난나컨씨탐마랏(나컨씨탐마랏 신화)과 히카얏 메롱 마하왕싸(메롱 마하왕싸 신화)를 중심으로 불교권 타이와 이슬람권 말레이의 문화적인 면을 고찰해보고자 한다. 본 논문에서는 땀난나컨씨탐마랏(나컨씨탐마랏 신화)와 히카얏 메롱 마하왕싸(메롱 마하왕싸 신화) 속에 나타난 두문화, 즉, 불교권 타이와 이슬람권 말레이의 문화는 동일한 구조를 보이고 있음을 밝히고, 그 구조속에 나타난 지도자의 역할과 그 지역의 발전에 종교가 중요한 요소로 작용하였음을 주장하고 있다. 이러한 주장의 바탕은 Levi-Strauss의 이론으로써 태국 남부의 불교권 타이문화와 말레이 북부의 이슬람권 문화가 얼마나 친밀한 문화적 뿌리를 가지고 있는 지를 알게 한다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.9
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• pp.3936-3951
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• 2011

This paper aims to analyze factors affecting technology transfer performance according to different types of Korean universities and to conduct the research for the channel and extent of impact between these factors, utilizing 5 - year data for the technology transfers of 110 universities based on the survey by National Research Foundation of Korea. According to the analysis, incentive for researchers is the most crucial factor in local universities and small & medium-sized private universities located in the capital area. And numerical value of intellectual property rights owned by university is the key factor in universities specializing in science & engineering / industry. Also, Big-sized universities are heavily affected by the number of full-time faculty. In case of private universities, government subsidy relating to patents is critical factor for technology transfer performance. The mean value of all variables is a lot higher in participant universities than non-participant ones in CK(Connect Korea) project. In summary, it is suggested that steady financial support provided by the government is required and that mutual cooperation for industry-university-government is also needed for the commercialization of the technologies held by universities.

• Korean Journal of Poultry Science
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• v.29 no.4
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• pp.237-241
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• 2002

To observe the effect of Induced molting on the recovery patterns of abdominal fat, visceral organs, and muscle tissues in spent laying hens after induced molting, three hundred sixty 77-wk-old, Babcock White hens were divided into 36 experimental units of 10 hens each and subjected to molt induction for seven wk. The post-molt production phase was spread over 84 to 126 wks of age. Thirty-six birds were randomly slaughtered and dressed at the pre-molt, 5% egg production, Peak, and end Phases of e99 Production. The body weight, abdominal fat, relative weight and length of visceral organs were measured. Proximate compositions of breast and thigh muscles were analyzed at each stage. The body weight was found to be minimal at the 5% egg Production stage, but increased as the egg Production increased for the rest of production. The pattern of abdominal fat change was very similar to that of body weight. The relative weight of the liver decreased to the lowest at the start of Post molting stage, but Peaked at the end Phase of egg Production (P<0.05). However, he heart and gizzard were observed to reach their maximum weight at the 5% egg Production (P<0.05), whereas they were, similar to those of the pre-molt phase for the rest of the production stages. Both intestine and reproductive tracts were found o be significantly smaller at 5% egg Production than at the other stages; however, their sizes increased gradually, reaching leak at the end Phase of e99 Production (P<0.05). Fat contents in breast and thigh muscles decreased significantly to the lowest at the start of the Post-molt stage, but increased to the highest at the end Phase of e99 Production (P<0.05). Thus, he Present study indicated that the molting process reduced body weight by decreasing the weights of abdominal fat and other visceral organs. Molting also influenced the breast and thigh muscle composition by decreasing fat content.

• Food Science and Preservation
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• v.15 no.3
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• pp.450-455
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• 2008

Previously, we have shown that green tea extract lowers the intestinal absorption of cholesterol, fat, and other fat-soluble compounds. We conducted this study to determine whether green tea extract affects the rate of $^{14}C$-oleic acid esterification into various lipids in the intestinal mucosa of rats. Male Sprague-Dawley ruts were had free access to a nutritionally adequate AIN-93G diet and deionized water. Initially, the rat's mucosal content of total lipids was measured following 1 mL olive oil administration with (green tea group) or without (control group) 100 mg green tea extract powder. At 1 h and 5 h, intestinal segments were extracted for total lipid analysis. Secondly, to measure mucosal esterification rates of lipids, an abdominal incision was made along the midline, and a 10-cm long jejunal segment of the small intestine was ligated in situ. Then, micellar solutions with or without green tea extract were injected into the ligated jejunal segments and incubated for 10 mill. The micellar solution contained $200.0\;{\mu}$ Ci $^{14}C$-oleic acid, $200.1\;{\mu}mol$ unlabelled oleic acid, $66.7\;{\mu}mol$ 2-monooleoylglycerol, $66.7\;{\mu}mol$ palmitoyl-sn-glycero-3-phosphocholine, 2.2 mmol glucose, $50.0\;{\mu}mol$ albumin, and 16.5 mmol Na-taurocholate per L of phosphate buffered saline (pH, 6.3) with or without 8.87 g green tea extract powder. At 10 min, each rat was sacrificed by cervical dislocation under anesthesia and the segment was removed for lipid analysis. Significant differences were observed in mucosal triglyceride content at 1 h and 5 h in ruts given green tea extract. Significant differences in the rate of $^{14}C$-oleic acid esterification into triglycerides and phospholipids fractions were observed between control and green tea groups. However, There were no significant differences in other lipid fractions. These results indicate that the lowered esterification rates of $^{14}C$-oleic acid into triglycerides and phospholipids fractions is attributable to presence of green tea extract. This may be associated with an inhibitory effect of green tea catechin on the mucosal processes of lipids, leading to the inhibition of intestinal absorption of lipids.

• Journal of Animal Environmental Science
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• v.15 no.1
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• pp.37-50
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• 2009

The purpose of this study was to investigate the effects of on-farm management systems(including the farm size, stocking density of growing-finishing phase, proportion of finisher diet and type of growing-finishing building) and pre-slaughter handling(including the transportation time and loading time) on carcass grade, the incidence of PSE pork, the meat quality score for intra-muscle fat, inter-muscle fat, subcutaneous fat and score for elasticity of market pigs. For this study, 248,787 pigs of 53 different farms were used to establish the pork quality assurance program and to meet the comsumer's need. The results are summarized as follows. 1. The farm size had significant influence on carcass grade, in which showing the higher grade by increasing the farm size. However, the incidence of PSE pork were not significantly differences among the farm size. The meat quality score for intra-muscle fat, inter-muscle fat, subcutaneous fat and score for elasticity of market pigs were not significant influenced by farm size. 2. The stocking density of growing-finishing phase was statistically significant for carcass grade, in which showing the higher grade in mid density group. However, the incidence of PSE pork was higher in high density group. The meat quality score for intra-muscle fat, inter-muscle fat and elasticity of market pigs were greater in mid density groups, but not influenced by stocking density for subcutaneous fat score. 3. The carcass grade and the incidence of PSE pork were not significantly influenced by transportation time. However, transportation time significantly affected the meat quality score, the meat quality score for intra-muscle fat, inter-muscle fat, subcutaneous fat and elasticity of the carcasses were superior in more than 1 hour transportation groups. 4. At any time loading, the carcass grade and PSE incidence were not significantly differences. However, the meat quality score for intra-muscle fat, inter-muscle fat and elasticity of market pigs were superior in before 10 AM groups, but not influenced by loading time for subcutaneous fat score. 5. The proportion of finisher diet had not significant influence on carcass grade, but PSE incidence affected by proportion of finisher diet, 21 percent or more group was higher PSE incidence. The meat quality score for intra-muscle fat and inter-muscle fat of the carcasses were superior in 21 percent or more fed finisher diet groups, but elasticity score of carcass had not influenced by the proportion of finisher diet. 6. The type of growing-finishing building was affected the carcass grade and PSE incidence of the market pigs, carcass grade and PSE incidence were superior in enclosed mechanical ventilation building groups. The meat quality score for intra-muscle fat and inter-muscle fat of the carcasses were not significantly differences by the type of finishing building, but the score of subcutaneous fat and elasticity of carcass were superior in opened natural ventilation building groups. In conclusion, the results of this study suggest that we have more precise on-farm management practice and the knowledge related to pre-slaughter handling skills to reduce the stress and improve the status of welfare of market pigs.