Browse > Article
http://dx.doi.org/10.3746/jkfn.2010.39.1.054

Effects of Baicalin on Gene Expression Profiles during Adipogenesis of 3T3-L1 Cells  

Lee, Hae-Yong (Dept. of Microbiology, Chung-Ang University College of Medicine)
Kang, Ryun-Hwa (Dept. of Microbiology, Chung-Ang University College of Medicine)
Chung, Sang-In (Dept. of Microbiology, Chung-Ang University College of Medicine)
Cho, Soo-Hyun (Dept. of Family Medicine, Yongsan Hospital, Chung-Ang University)
Yoon, Yoo-Sik (Dept. of Microbiology, Chung-Ang University College of Medicine)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.1, 2010 , pp. 54-63 More about this Journal
Abstract
Baicalin, a flavonoid, was shown to have diverse effects such as anti-inflammatory, anti-cancer, anti-viral, anti-bacterial and others. Recently, we found that the baicalin inhibits adipogenesis through the modulations of anti-adipogenic and pro-adipogenic factors of the adipogenesis pathway. In the present study, we further characterized the molecular mechanism of the anti-adipogenic effect of baicalin using microarray technology. Microarray analyses were conducted to analyze the gene expression profiles during the differentiation time course (0 day, 2 day, 4 day and 7 day) in 3T3-L1 cells with or without baicalin treatment. We identified a total of 3972 genes of which expressions were changed more than 2 fold. These 3972 genes were further analyzed using hierarchical clustering analysis, resulting in 20 clusters. Four clusters among 20 showed clearly up-regulated expression patterns (cluster 8 and cluster 10) or clearly down-regulated expression patterns (cluster 12 and cluster 14) by baicalin treatment for over-all differentiation period. The cluster 8 and cluster 10 included many genes which enhance cell proliferation or inhibit adipogenesis. On the other hand, the cluster 12 and cluster 14 included many genes which are related with proliferation inhibition, cell cycle arrest, cell growth suppression or adipogenesis induction. In conclusion, these data provide detailed information on the molecular mechanism of baicalin-induced inhibition of adipogenesis.
Keywords
baicalin; adipogenesis; microarray; gene expression profile;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Willard J, Vicanek C, Battaile KP, Van Veldhoven PP, Fauq AH, Rozen R, Vockley J. 1996. Cloning of a cDNA for short/branched chain acyl-coenzyme A dehydrogenase from rat and characterization of its tissue expression and substrate specificity. Arch Biochem Biophys 331: 127-133.   DOI
2 Nadal A, Marrero PF, Haro D. 2002. Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1. Biochem J 366: 289-297.   DOI
3 de Boer VC, van Schothorst EM, Dihal AA, van der Woude H, Arts IC, Rietjens IM, Hollman PC, Keijer J. 2006. Chronic quercetin exposure affects fatty acid catabolism in rat lung. Cell Mol Life Sci 63: 2847-2858.   DOI
4 Kato MV, Sato H, Tsukada T, Ikawa Y, Aizawa S, Nagayoshi M. 1996. A follistatin-like gene, mac25, may act as a growth suppressor of osteosarcoma cells. Oncogene 12: 1361-1364.
5 Kanemitsu N, Kato MV, Miki T, Komatsu S, Okazaki Y, Hayashizaki Y, Sakai T. 2000. Characterization of the promoter of the murine mac25 gene. Biochem Biophys Res Commun 279: 251-257.   DOI
6 Guttridge KL, Luft JC, Dawson TL, Kozlowska E, Mahajan NP, Varnum B, Earp HS. 2002. Mer receptor tyrosine kinase signaling: prevention of apoptosis and alteration of cytoskelosil architecture without stimulation or proliferation. J Biol Chem 277: 24057-24066.   DOI
7 Shimomura I, Hammer RE, Richardson JA, Ikemoto S, Bashmakov Y, Goldstein JL, Brown MS. 1998. Insulin resistance and diabetes mellitus in transgenic mice expressing nuclear SREBP-1c in adipose tissue: model for congenital generalized lipodystrophy. Genes Dev 12: 3182-3194.   DOI
8 Zhang M, Ikeda K, Xu JW, Yamori Y, Gao XM, Zhang BL. 2009. Genistein suppresses adipogenesis of 3T3-L1 cells via multiple signal pathways. Phytother Res 23: 713-718.   DOI
9 Darlington GJ, Ross SE, MacDougald OA. 1998. The role of C/EBP genes in adipocyte differentiation. J Biol Chem 273: 30057-30060.   DOI
10 Schoonjans K, Peinado-Onsurbe J, Lefebvre AM, Heyman RA, Briggs M, Deeb S, Staels B, Auwerx J. 1996. PPARalpha and PPARgamma activators direct a distinct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene. Embo J 15: 5336-5348.
11 Cornelius P, MacDougald OA, Lane MD. 1994. Regulation of adipocyte development. Annu Rev Nutr 14: 99-129.   DOI
12 Gregoire FM, Smas CM, Sul HS. 1998. Understanding adipocyte differentiation. Physiol Rev 78: 783-809.   DOI
13 Lockhart DJ, Dong H, Byrne MC, Follettie MT, Gallo MV, Chee MS, Mittmann M, Wang C, Kobayashi M, Horton H, Brown EL. 1996. Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat Biotechnol 14: 1675-1680.   DOI
14 Chan FL, Choi HL, Chen ZY, Chan PS, Huang Y. 2000. Induction of apoptosis in prostate cancer cell lines by a flavonoid, baicalin. Cancer Lett 160: 219-228.   DOI
15 Tamamori-Adachi M, Goto I, Yamada K, Kitajima S. 2008. Differential regulation of cyclin D1 and D2 in protecting against cardiomyocyte proliferation. Cell Cycle 7: 3768-3774.   DOI   ScienceOn
16 Hao J, Varshney RR, Wang DA. 2008. TGF-beta3: A promising growth factor in engineered organogenesis. Expert Opin Biol Ther 8: 1485-1493.   DOI
17 Hanington PC, Barreda DR, Belosevic M. 2006. A novel hematopoietic granulin induces proliferation of goldfish (Carassius auratus L.) macrophages. J Biol Chem 281: 9963-9970.   DOI
18 Seelinger G, Merfort I, Schempp CM. 2008. Anti-oxidant, anti-inflammatory and anti-allergic activities of luteolin. Planta Med 74: 1667-1677.   DOI
19 Middleton E Jr, Kandaswami C, Theoharides TC. 2000. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev 52: 673-751.
20 Li BQ, Fu T, Dongyan Y, Mikovits JA, Ruscetti FW, Wang JM. 2000. Flavonoid baicalin inhibits HIV-1 infection at the level of viral entry. Biochem Biophys Res Commun 276: 534-538.   DOI
21 Liu IX, Durham DG, Richards RM. 2000. Baicalin synergy with beta-lactam antibiotics against methicillin-resistant Staphylococcus aureus and other beta-lactam-resistant strains of S. aureus. J Pharm Pharmacol 52: 361-366.   DOI
22 Lee H, Kang R, Hahn Y, Yang Y, Kim SS, Cho SH, Chung SI, Yoon Y. 2009. Antiobesity effect of baicalin involves the modulation of proadipogenic and antiadipogenic regulators of the adipogenesis pathway. Phytother Res DOI:10.1002/ptr.2937.   DOI
23 Zeeberg BR, Feng W, Wang G, Wang MD, Fojo AT, Sunshine M, Narasimhan S, Kane DW, Reinhold WC, Lababidi S, Bussey KJ, Riss J, Barrett JC, Weinstein JN. 2003. GoMiner: a resource for biological interpretation of genomic and proteomic data. Genome Biol 4: R28.   DOI
24 Rosen ED, MacDougald OA. 2006. Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 7: 885-896.   DOI
25 Saito T, Abe D, Sekiya K. 2008. Sakuranetin induces adipogenesis of 3T3-L1 cells through enhanced expression of PPARgamma2. Biochem Biophys Res Commun 372: 835-839.   DOI   ScienceOn
26 Lee J, Jung E, Lee J, Kim S, Huh S, Kim Y, Kim Y, Byun SY, Kim YS, Park D. 2009. Isorhamnetin represses adipogenesis in 3T3-L1 cells. Obesity (Silver Spring) 17: 226-232.   DOI
27 Fajas L. 2003. Adipogenesis: a cross-talk between cell proliferation and cell differentiation. Ann Med 35: 79-85.   DOI
28 Adams CM, Reitz J, De Brabander JK, Feramisco JD, Li L, Brown MS, Goldstein JL. 2004. Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs. J Biol Chem 279: 52772-52780.   DOI
29 Saxena N, Banerjee S, Sengupta K, Zoubine MN, Banerjee SK. 2001. Differential expression of WISP-1 and WISP-2 genes in normal and transformed human breast cell lines. Mol Cell Biochem 228: 99-104.   DOI
30 Glickstein SB, Monaghan JA, Koeller HB, Jones TK, Ross ME. 2009. Cyclin D2 is critical for intermediate progenitor cell proliferation in the embryonic cortex. J Neurosci 29: 9614-9624.   DOI
31 Guo X, Liao K. 2000. Analysis of gene expression profile during 3T3-L1 preadipocyte differentiation. Gene 251: 45-53.   DOI
32 Havsteen B. 1983. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol 32: 1141-1148.   DOI
33 Ouafik L, Berenguer-Daize C, Berthois Y. 2009. Adrenomedullin promotes cell cycle transit and up-regulates cyclin D1 protein level in human glioblastoma cells through the activation of c-Jun/JNK/AP-1 signal transduction pathway. Cell Signal 21: 597-608.   DOI