• Title/Summary/Keyword: 지방산 F-O

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Characterization on Fatty Acids and Amino Acids of Quality Protein Maize Lines (Quality Protein Maize 육성계통의 지방산 및 아미노산 특성)

  • Kim Sun-Lim;Son Beom-Young;Jung Tae-Wook;Moon Hyun-Guey;Son Jong-Rok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.5
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    • pp.458-465
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    • 2006
  • This study was carried out to evaluate the nutritional properties of quality protein maize (QPM) and to select the elite lines for corn breeding. Two laboratory procedures for simultaneous identification of QPM and lysine content analysis were performed. The $BC_{1}F_{2}$ lines of KS5/QPM and KS135/QPM were analyzed with opaque-2(o2) specific SSR marker in order to differentiate the opaque-2 carrying QPM lines from the non-opaque genotypes. Although no significant difference in protein content, significant differences in lipid, ash, and crude fiber contents were observed. The composition of unsaturated fatty acid of QPM lines was slightly lower than non-QPM lines, but there was no significant difference. Sulphur-containing amino acids such as methionine and cystine showed no difference between QPM and non-QPM lines. However, lysine content of QPM lines was 38% higher than that of non-QPM lines, and the essential amino acid content of QPM lines (28.1%) was higher compared to non-QPM lines (27.1%).

Antioxidant Activity and Fatty Acid Aomposition of Beach Rose (Rosa rugosa) Fruit and Seed from Various Ripening Stages (해당화 열매 과육과 종실의 숙기별 항산화능 및 지방산 조성)

  • Chae, Sung Hun;Song, Hun Jong;Lee, Young Sang;Han, Tae-Ho;Ku, Kang-Mo
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.69-69
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    • 2019
  • 해당화 열매를 숙기 별로 과육의 항산화물질 함량과 씨앗의 지방산 함량을 측정하여 평가하였다. 과육의 1,1-diphenyl-2-picrylhydrazyl (DPPH) 항산화능을 갈산 환산농도로 나타내면 미성숙 과육은 $4.36{\pm}0.03mg/g$, 성숙 초기 과육은 $4.54{\pm}0.37mg/g$, 성숙 중기 과육에는 $4.78{\pm}0.21mg/g$, 성숙 후기에는 $4.30{\pm}0.22mg/g$ 범위로 항산화능을 보여주었다. 항산화능력은 성숙중기에서 높은 함량을 보여주었고 성숙함에 따라 감소함을 보여주었다. 미성숙, 성숙 초기는 유의한 걸로 나오지만 성숙 중기와 성숙 후기은 다른 성숙도와 유의하지 않았다. 총 페놀 함량의 미성숙 과육은 $1.60{\pm}0.03mg/g$, 성숙 초기 과육은 $1.80{\pm}0.08mg/g$, 성숙 중기 과육에는 $2.03{\pm}0.67mg/g$, 성숙 후기에는 $1.53{\pm}0.07mg/g$ 범위로 폴리페놀이 존재하였다. 성숙 초기에 제일 높은 항산화능력과 폴리페놀 함량을 보여주고 있으며 성숙할수록 항산화능력과 총 페놀 함량이 떨어지는 것을 알 수 있다. 씨앗의 지방산 함량은 리올레산, 리놀렌산, 올레산, 팔미트산 순으로 많았다. 리올레산은 미성숙 씨앗은 전체 함량 중 56.46%, 초기 과육 씨앗에는 54.26%, 중기 과육 씨앗에는 51.67%, 후기 씨앗에는 50.75%의 함량을 가지고 있다. 리올렌산은 미성숙 씨앗은 전체 함량 중 18.80, 초기 과육 씨앗에는 17.96%, 중기 과육 씨앗에는 18.90%, 후기 씨앗에는 21.42%의 함량을 가지고 있다. 올레산은 미성숙 씨앗은 전체 함량 중 13.54%, 초기 과육 씨앗에는 20.78%, 중기 과육 씨앗에는 22.44%, 후기 씨앗에는 20.81%의 함량을 가지고 있다. 팔미트산은 미성숙 씨앗은 전체 함량 중 6.80%, 초기 과육 씨앗에는 3.40%, 중기 과육 씨앗에는 3.37%, 후기 씨앗에는 3.32%의 함량을 가지고 있다. 리올레산은 미성숙 시기에 가장 많았고 리올렌산은 후기에 올레산은 중기에, 팔미트산은 초기에 많았다.

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Studies on the Fatty Acid Composition of Amorpha-fruticosa Seeds (Amorpha-fruticosa종자(種子)의 지방산조성(脂肪酸組成)에 관한 연구(硏究))

  • Whang, Byung-Ho;Lee, Sang-Young
    • Korean Journal of Food Science and Technology
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    • v.6 no.2
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    • pp.86-90
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    • 1974
  • Quantitative analysis of the fatty acids contained in Amorpha-fruticosa seeds was carried out by means of gas chromatography with F.I.D. The general components and chemical constants have been performed with A.O.A.C methods. The results are summarized as follow: 1. General components of Amorpha-fruticosa seeds come out to be 17.65% moisture, 21.02% crude protein, 12.04% crude lipid and 5.37% ash. 2. Extraction of crude lipids were performed by soxhlet extractor for 14 hour. Amounts of the crude lipids were extracted 80.25% in ether, 80.00% in methanol, 77.34% in benzene and 69.96% in hexane. 3. Chemical constants of Amorpha-fruticosa seed oil were saponification number 178.67, acid number 3.11 and iodine number 54.27. 4. The fatty acid components of Amorpha-fruticosa seeds were quantitatively determined by gas chromatography to give 78.73wt% linoleic, 5.8wt% oleic, 5.68wt% palmitic, 4.8wt% stearic and 3.40wt% linolenic acid in ether solvent and to give 77.86wt% linoleic, 7.77wt% palmitic, 5.84wt oleic and 4.97wt% stearic acid in methanol solvent. The peak of capric acid was not found. Myristic, arachidic and lauric acids were very small.

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Effect of Egg Yolks from Laying Hens Intubated Astaxanthin on the Oxidation of Liver Microsome of Mouse (Astaxanthin처리 산란계로부터 생산된 난황이 Mouse Liver Microsome의 산화에 미치는 영향)

  • 김홍출;박숙자;김정곤;박철우;조용운;조현종;하영래
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.1
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    • pp.155-159
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    • 2002
  • Effect of the egg yolky from laying hens intubated, p.o., astaxanthin (designated AEY) on the oxidation of mouse liver microtome was investigated using female ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 7 treatment groups (10 mice/cage/treatment). Mice were intubated p.o., AEY (50, 100 and 150 mg) or control egg yolk (CEY, 150 mg) every week for 3 weeks. BHT (5 mg) and e -tocopherol (50 mg) were fed to mice as positive control. At week 4, the liver microsome was prepared from sacrificed mice. Protein content of mouse liver microsome with AEY treatment was relatively higher than that with CEY treatment. AEY treatment remarkably lowered the content of unsaturated fatty acids including oleic acid and linoleic acid, but raised that of the saturated fatty acids including stearic acid. AEY group showed relatively higher antioxidative activity than CEY, when used Asc/F $e^{+2}$ or NADPH/F $e^{+2}$ as oxidant. Antioxidative activity of AEY was more effective than $\alpha$-tocopherol, but less effective than BHT.

Effect of Additive, Storage Temperature and Time on the Texture Properties of Baikseolgi (첨가물, 저장온도 및 저장시간에 따른 백설기의 텍스쳐 특성)

  • Kim, Myung-Hwan
    • Applied Biological Chemistry
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    • v.41 no.6
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    • pp.437-441
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    • 1998
  • The effects of sucrose fatty acid ester (SE, 1% w/w) and glycerin (GL, 1% v/w) additions, storage temperature$(0,\;20\;and\;70^{\circ}C)$, and time $(0{\sim}6\;day)$ on texture properties, hardness(H), cohesiveness(O), chewiness(C) and rheological property(R) of Baikseolgi were studied. The H of Baikseolgi increased sharply in the early stage of storage at 0 and $20^{\circ}C$, while increased gently at $70^{\circ}C$ with increasing storage time. After 6 days of storage, the H of Baikseolgi at $20^{\circ}C$ had a little lower than that at $0^{\circ}C$. However, the H of Baikseolgi at $70^{\circ}C$ was 10.7% of that at $0^{\circ}C$. The addition of GL had greater effect on the reduction of H than that of SE. The H of control, SE and GL additions were 336, 216 and $$174\;g_f, respectively, after 6 days at $70^{\circ}C$. The O of Baikseolgi at $70^{\circ}C$ were higher than those at $0^{\circ}C$. The O of GL added Baikseolgi had the highest value and the second and the third were SE added and control, respectively. The O of Baikseolgi decreased with increasing storage time. The C of Baikseolgi of increased with increasing storage time, which had similar curve patterns to the H of Baikseolgi. Instantaneous stress and equilibrium stress of Baikseolgi decreased with increasing storage temperature. The affection of viscous element increased and that of elastic element decreased with increasing storage temperature.

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Development of Flower Color Changed Landscape Plant through Interspecific and Intergeneric Crosses of Several Cruciferae Crops (십자화과 작물의 종속간 교배를 통한 화색변화 경관용 자원식물 개발)

  • Kim, Kwang-Soo;Park, Won;Lee, Yong-Hwa;Lee, Ji-Eun;Moon, Youn-Ho;Cha, Young-Lok;Song, Yeon-Sang
    • Korean Journal of Plant Resources
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    • v.31 no.1
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    • pp.77-85
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    • 2018
  • The cultivation area of rapeseed (Brassica napus L.) has been increased for oil production and landscaping purpose in Korea. However, as the color of rapeseed flower is very simple, diversified flower color is necessary to improve landscape effect. Interspecific and intergeneric crosses between rapeseed (Brassica napus) and three Cruciferae crops were performed in order to grow diverse flower color of rapeseed. The silique formation rate of interspecific cross rapeseed with cabbage (B. oleracea L) was relatively high (65.8%) and higher than intergeneric cross with rapeseed and radish (Raphanus sativus L.), rapeseed and Orychophragmus, respectively. During silique developing period after artificial pollination, there were many siliques without seeds due to the failure of fertilization. The average number of seed per silique obtained from cross rapeseed and cabbage, rapeseed and radish, rapeseed and O. violaceus were 0.12, 0.4 and 0.12, respectively. The phenotypes of $F_1$ hybrid plants from cross rapeseed and Cruciferae crops were mostly similar to maternal line, but leaf length and leaf width were increased. The interspecific cross of rapeseed and cabbage generated ivory color of flower which is the medium color of parents, and intergeneric cross of rapeseed and O. violaceus created entities with larger flowers which seems to enhance landscape effect. The fatty acid composition of most hybrid seeds intermediated of the two parents for oleic acid, linoleic acid and linolenic acid, content. Whereas hybrid of rapeseed and radish produced less erucic acid than radish parent.

Funtional Components of Holophyte - Antioxidant substances in Salicornia herbacea L. - (염생식물의 기능 - 퉁퉁마디(S.hrebacea)의 항산화능 -)

  • Kim, Jong-Bae;Choe, Sun-Nam;Choe, Kyu-Hong;Lim, Seong-Han;Chai, Suk-Jin
    • Journal of Fisheries and Marine Sciences Education
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    • v.19 no.2
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    • pp.197-205
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    • 2007
  • This study was to investigate the chemical content and antioxidant activity by the part of Salicornia herbacea as part of using Salicornia herbacea as materials of functional foods. On a basis of the materials, this study was to clarify the nutritional excellence, chemical composition, and antioxidant activity of Salicornia herbacea. From the result of this research above, it may be summed up as follows.The Salicornia herbacea used in the experiment contained the moisture and crude fiber in the stalks a lot, and the protein, fat, and ash in the branches more. As to the content of free amino acid, the content of arginine took in the branches and stems most. Then, it contained threonine, glycine, tryptophan, valine, isoleucine, and lysine, etc. in the branches a lot. Also, it contained threonine, glutamic acid, tyrosine tryptophan alanine, and isoleucine in the stalks a lot. It was shown to be contained of essential amino acids like isoleucine, leucine, threonine, valine, methionine, lysine, phenylalanine, and tryptophan in the branches and steams more. As to the content of fatty acid in Salicornia herbacea, it found out that it contained the unsaturated fatty acid more than the saturated fatty acid. It took the content of eicosenoic acid(20:1) in the branches and stalks most. And then, it contained linoleic acid(18:2), pehtadecenoic acid(15:1), palmitic acid(16:0), and oleic acid(18:1), etc. a lot. As to the antioxidant activity in Salicornia herbacea using the DPPH radical, it was shown to be existed in the largest antioxidant activity when the concentration of methanolextract from Salicornia herbacea was 1 mM. There was higher antioxidant activity than 100 ppm BHT used as control plot when the concentration of methanol extract from the stalks was $100{\mu}M$. From the result of experiment above, Salicornia herbacea contained the essential amino acid a lot. It will be possible to be used as natural antioxidants because it has excellent antioxidant effect. Therefore, this researcher concludes that it will be available in using it as materials of functional foods.

Analysis of Quantitative Trait Loci (QTLs) for Unsaturated Fatty Acid Contents in Soybean Seed Using Recombinant Inbred Lines (콩에서 microsatellite marker를 이용한 불포화지방산 함량의 양적형질 유전자좌의 분석)

  • Kim, Hyeun-Kyeung;Im, Moo-Hyeog;Choung, Myoung-Gun
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1665-1670
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    • 2008
  • Soybean oil is an important source of vegetable oil for human food and nonfood applications and accounts for approximately 22% of the world's total edible oil production. Improvement of the quality and quantity of soybean seed oil constituents is one of the most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control oleic, linoleic, and linolenic acid contents in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.85, 0.82 and 0.81, respectively. Eight independent QTLs for oleic acid content were identified from linkage group (LG) A2, C1, D2, F, G, L, and O. Seven QTLs for linoleic acid content were located on LG D1b, E, H, I and L. Oil content was related with five QTLs located on LG C1, H, J, K, and L. Oleic, linoleic, and linolenic acid have two common QTLs on LG C1 and L. Thus, we identified major loci improving soybean oil quality.

Petrochemical study on the Daejeon-sa basalt in the Mt. Juwang area, Cheongsong (청송 주왕산지역 대전사 현무암의 암석화학적 연구)

  • 윤성효;이문원;고정선;김영라;안지영
    • The Journal of the Petrological Society of Korea
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    • v.9 no.2
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    • pp.84-98
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    • 2000
  • Daejeon-sa basalt in the Mt. Juwang area composed of 12 basalt flows alternate with 9 peperites and each basalt and peperite has the variety of thickness. Peperites yielded in Daejeon-sa basalt are mixed of basalt with reddish shale, of which textural type is globular peperite. Basalts yielded in Daejeon-sa basalt are massive basalt without vesicule, although sometimes vesicules are founded in upper within a flow unit. The basalt has mainly pseudomorph of olivine as phenocryst, and also plagioclase and clinopyroxene phenocryst. Matrix is mainly subophitic texture. The plotting result on the TAS diagram shows these basalts belong to the sub-alkaline, and it can be subdivided into calc-alkaline series on the basis of the diagram of Si02 vs. K20 and of alkali index vs. A1203 diagram. According to plots of wt.% oxides vs. wt.% MgO, abundances of A1203 and CaO increase with decreasing MgO while F ~ dOecre~ase . With decreasing MgO compatible elements decrease while incompatible elements increase. In spider diagram of MORB-normalized trace element patterns, HFS elements are nearly similiar with MORB, but LIL elements are enriched. Especially, contents of Ce, F: and Sm are enriched but Nb is depleted. In the chondrite-normalized REE patterns light REEs are enriched than heavy REEs. Tectomagmatic discrimination diagrams shows basalts in the study area are formed in the tectonomagmatic environment of subduction zone under continental margin. This result accord with characters of chemical composition mentioned above. Cr vs. Y diagram and CeM, vs. Ce diagram show that the primary magma of the basalts may formed by the about 15% partial melting of garnet-peridotite in the mantle wedge. After then, Daejeon-sa basalts may formed from evolved magma undergone mainly olivine fractional crystallization and contarnination of crustal materials before eruption.

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Amino Acid Composition and Scanning Electron Micrographs of Modified Silkworm Larvae Protein Concentrates (변형번데기 농축단백질 아미노산 조성 및 주사전자현미경에 의한 관찰)

  • 박금순;박정륭
    • Korean journal of food and cookery science
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    • v.3 no.2
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    • pp.9-16
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    • 1987
  • This study was designed to determine the amino acid composition and to investigate microstructure by scanning electron micrographs of silkworm larvae protein and modified silkworm larvae protein concentrate. The results were as follows: 1, The protein contents of soybean and silkworm larvae protein concentrate were 70.3% and 84.1%, respectively. 2. In general, the essential amino acid content of silkworm larvae protein concentrate were higher than soybean protein concentrate as well as FAO provisional scoring pattern. Silkworm larvae protein concentrate was especially high in lysine and methionine indicating that it could be a good supplemental source for cereals and beans. Succinylation and acetylation resulted in no difference in most amino acid content. 3. The scanning electron microscopic observations revealed that silkworm larvae protein concentrate had smooth surface topography while defatted silkworm larvae flour showed different shapes and sizes with relatively rough surfaces. Acylated silkworm larvae protein concentrate exhibited less cellularity and denser than protein concentrate. However, succinylated silkworm larvae protein concentrate showed especially good texture indicating that it could increase the functional properties of silkworm larvae protein concentrate.

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