• Title/Summary/Keyword: 지방산 함량

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Fractionation and Quantitative Analysis of Lipid Components in Korean Yam (Dioscorea) Tubers (한국산 마의 지질 성분의 분리 및 분석)

  • Chung, Hae-Young
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.509-515
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    • 1994
  • Using the lipids extracted from Korean yam(Dioscorea) tubers, D. batatas, D. aimadoimo and D. japonica, fractionation and identification of lipid components and their fatty acid compositions were analysed. Lipid contents determined by Folch's method in D. batatas, D. aimadoimo and D. japonica were 11.0 mg/g, 11.4 mg/g and 6.6 mg/g, respectively. Lipids extracted were fractionated into neutral lipid, glycolipid and phospholipid by silicic acid column chromatography. The content of neutral lipid was over about 60% in lipid. Neutral lipid was composed of sterol ester, triglyceride, 1,3-diglyceride, 1,2-diglyceride and monoglyceride. Main constituents of glycolipid were acylsterylglycoside, monogalactosyldiglyceride, sterylglycoside, digalactosyldiglyceride and sulfolipid, and phospholipid contained phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. The fatty acids of the total lipid and its three lipid fractions were analyzed by GC. The major fatty acids were palmitic and linoleic acids. Content of the saturated fatty acids was less than that of the unsaturated fatty acids.

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Effects of Nitrogen Level and Planting Density on Chemical Properties in Korean Native Tabacco Varieties (재래종연초의 화학특성에 미치는 질소시비량 및 재식밀도의 영향)

  • 정형진;김길웅;박수준
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.3
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    • pp.288-295
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    • 1989
  • This study was conducted to establish the most appropriate nitrogen levels and planting densities which can attribute to increase of aromatic substances in tobacco variety, Hyangcho, maintaining its original aroma. An increase of nitrogen fertilization from 3 to 12 kg per 10a increased total alkaloid content 2.0% to 3.3%, while an increased nitrogen fertilization decreased the contents of total sugar, starch, fatty acid etc., Which seem to be the positive components relating to tobacco aroma the contents of alkaloids were decreased and total fatty acids were increased as the planting densities increased. The higher contents of 2. 3-methylbutanoic and 3-methylpentanoic acid was obtained at the density of 3 plant per pit (12,498 plant/10a). The components determined in the neutural fraction such as solanone, furfural, furfurylalcohol and benzyl alcohol were presented in higher amount at the higher densities. The volatile acids such as 2. 3-methylbutanoic acid 3-methylpentanoic acid decreased and also the contents of butanol, furfutal, furfuryl alcohol, hexanol, cinnamil, acetophenone, benzyl acetate and solanone etc., were decreased as the nitrogen level increased from 3 to 12 kg per l0a.

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Determination of Seed Fatty Acids Using Near-Infrared Reflectance Spectroscopy(NIR) in Mung Bean(Vigna radiata) Germplasm (녹두 유전자원 지방산 함량 대량평가를 위한 근적외선분광법의 적용)

  • Lee, Young-Yi;Kim, Jung-Bong;Lee, Sok-Young;Kim, Min-Hee;Lee, Jung-Won;Lee, Ho-Sun;Ko, Ho-Cheol;Hyun, Do-Yoon;Gwag, Jae-Gyun;Kim, Chung-Kon;Lee, Yong-Beom
    • The Korean Journal of Food And Nutrition
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    • v.23 no.4
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    • pp.582-587
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    • 2010
  • 본 연구에서는 녹두 유전자원의 지방산 함량을 신속 대량 검정하는 기술을 개발하여 유전자원 활용 및 육종 촉진에 기여하고자 하였다. 유전자원 평가에 적합한 신속하고 비파괴적인 지방산 함량 평가기술을 개발하기 위해 공시자원 1,125점의 녹두 종자를 종실상태와 분쇄한 분말상태로 근적외선분광분석기(NIR)를 이용하여 1,104~2,494 nm에서의 스펙트럼을 얻고 이들 중 스펙트럼이 중복되지 않는 원산지가 다양한 대표자원 106점을 선발하여 일반적인 방법으로 지방산 함량을 분석하고, 이 값과 NIR 스펙트럼 흡광도값 간의 상관분석을 위한 calibration set로 활용하였다. 그 결과 palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid 및 total fatty acid에 대한 NIR 흡광도와의 상관계수 $R^2$이 각각 0.74, 0.18, 0.12, 0.72, 0.48 및 0.78로 나타났고, 이들 중 $R^2$가 높은 검량식을 미지의 시료 10점으로 검증한 결과, palmitic, linoleic 및 total fatty acid에 대한 검증 상관계수 $R^2$이 0.96, 0.74, 0.81로 나타나, 다양한 녹두 유전자원의 지방산함량 신속 대량 예측에 유효하게 활용될 수 있는 것으로 나타났다. 한편, 공시된 녹두 유전자원 115점 중에서 자원번호 IT208075 자원은 저 지방산 자원($14.24\;mg\;g^{-1}$)으로 선발되었고, IT163279 자원은 고 지방산 자원($18.43\;mg\;g^{-1}$)으로 선발되어 향후 녹두작물의 성분육종에 유용할 것으로 생각된다.

Changes of Amino Acid, Fatty Acid and Lipid Composition by the Growth Period in Velvet Antler (녹용의 성장 기간에 따른 아미노산, 지방산, 지질성분의 변화)

  • Jeon, Byong-Tae;Moon, Sang-Ho;Lee, Sang-Rak;Kim, Myeong-Hwa
    • Food Science of Animal Resources
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    • v.30 no.6
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    • pp.989-996
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    • 2010
  • This study was aimed at investigating the differences in chemical composition of antler according to growing days (80 d and 90 d after casting) and sections (upper, middle, base) in elk. Antlers of six bulls (aged 3 years) cutted on day 80 and 90 after casting were used in this trial and compared chemical composition such as crude protein, crude fat, crude ash, calcium, magnesium, amino acids, lipids, and fatty acids. The contents of crude protein and ether extract were higher in antlers cutted on day 80 than in antlers cutted on day 90, but that of crude ash was higher in antlers cutted on day 90. Significantly difference was observed in crude protein content between growing days in middle part of antler (p<0.05). Calcium and magnesium contents were higher on 90 d than 80 d, but there was no significant difference between growing days of antler. Based on the growing period of antler, there were significant differences (p<0.05) in aspartic acid, threonine, valine, leucine, phenylalanine and lysine, and essential amino acids content was higher in antlers cutted on day 80. Total lipid, neutral lipid, and phospholipid contents were higher in antlers cutted on day 80 than in those cutted on day 90, sphingo-phospholipid content was higher than glycerophospholipid content and values was higher in antlers cutted on day 80 than in those cutted on day 90. For total fatty acids, unsaturated fatty acids composition, there was no significant difference between growing periods of antlers, but values was higher on day 80 than on day 90. Essential fatty acids contents were lower in antler cutted on day 90 than antlers cutted on day 80 and higher for upper part than lower part.

Changes in Contents of Ginsenosides, Free Sugars and Fatty Acids in Developing Ginseng Seed (인삼종자 생장과정에서 사포닌, 유리당 및 지방산의 변화)

  • 이종철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.2
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    • pp.134-137
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    • 1988
  • Sizes of ginseng seeds and contents of ginsenosides, free sugars and fatty acids in the seeds were investigated at different dates after flowering of 4 year old ginseng to get basic information used for determining harvest . time of ginseng seed. The sizes of seeds were maximum about 35 days after flowering(DAF), while those of endosperms reached maximum at 50 DAF. At 65 DAF seeds with intact pulp weighed most heavy. The amounts of total saponin and ginsenosides were decreased with time after flowering. Contents of free sugars such as glucose, maltose and fructose were decreased continously after flowering. Amount of palmic acid was decreased, .but those of oleic, linoleic and stearic acids were increased with time after flowering.

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Analysis of Trans Fatty Acid Content in Processed Foods and Meat Products (가공식품과 육가공품의 트랜스 지방산 함량 조사)

  • Park, Jung-Min;Ji, Won-Gu;Kim, Eun-Jung;Park, Da-Jung;Shin, Jin-Ho;Shim, Soon-Mi;Suh, Hyung-Joo;Chang, Un-Jae;Kang, Duk-Ho;Kim, Jin-Man
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.531-537
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    • 2007
  • Small amounts of trans fatty acids exist naturally in beef and dairy foods. Also, they can be produced in the process of partial hydrogenation to manufacture shortning or margarine. They can provide a better palatability and shelf life. According to the recently studies, trans fatty acids can raise health risk such as heart diseases and coronary artery diseases. They can also increase low-density lipoprotein (LDL) cholesterol and decrease high-density lipoprotein (HDL) cholesterol in the blood plasma, therefore increasing the risk of atherosclerosis and diabetes. The aim of this study was to determine total lipids and trans fatty acids (TFAs) content in processed foods and meat products. The analysis of trans fatty acids was performed in 28 samples of donuts, 18 samples of bakeries, 4 samples of frozen doughs, 2 samples of popcorns, and 4 samples of meat products (ham, sausage, nuget, and bacon). Total lipids in processed foods and meat products were extracted by chloroform-Methanol method and acid digestion, respectively. They were analyzed by gas chromatography using a SP-2560 column and flame ionization detector. The amounts of TFAs per 100 g of foods were 0-3.3% (0.74% on average) in donuts, 0.2-5.8% (1.18% on average) in bakeries, 0.2-6.3% (1.93% on average) in frozen doughs, and 0-5.8% in popcorns. Meat products such as ham, sausage, and nuget analyzed 0.1% of TFAs, respectively and trans fatty acids in bacon were not detected. As a result, the distribution of TFAs in processed foods was widely ranged from O% to 6.3% according to manufacturers and types of products, whereas the content of TFAs in meat products ranged from 0% to 0.1%.

Quality Characteristics of Commercial Slices of Skate Raja kenojei (국내산과 수입산 시판 홍어회의 이화학적 및 미생물학적 품질 특성에 관한 연구)

  • Cho, Hee-Sook;Kim, Kyung-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.2
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    • pp.214-220
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    • 2008
  • The purpose of this study was to investigate the quality characteristics of domestic and imported slices of skates Raja kenojei. Four types of slices of skates Raja kenojei were analyzed for proximate composition, extractive nitrogen, free amino acids, and fatty acids. A large amount of TMAO was detected in the domestic slices of skate. Both the domestic and imported skate contained aerobic bacteria, with approximate levels of 6.6 log CFU/g and 5.1${\sim}$6.5 log CFU/g, respectively. The total free amino acids contents of the domestic and imported skates were 579.7 mg and 387.6${\sim}$496.3 mg, respectively; all samples had high levels of taurine, anserine, lysine, alanine, glycine, proline, ${\beta}$-alanine, and histidine. Eleven different saturated fatty acids were found in the domestic skates whereas the impored skate had 7${\beta}$10 different types of saturated fatty acid. In addition, 16 kinds of domestic skate, and 10${\beta}$15 kinds of imported skate were analyzed for unsaturated fatty acid content. From the overall results, the domestic skate proved to be a better source of amino acid, and had higher levels of aerobic bacteria and fatty acids than the imported skate.

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Effect of Additions of Supplemental Activated Carbon on the Fatty Acid, Meat Color and Minerals of Chicken Meat (활성탄의 첨가가 계육의 지방산, 육색 및 무기물에 미치는 영향)

  • 박창일;김영직
    • Food Science of Animal Resources
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    • v.21 no.4
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    • pp.285-291
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    • 2001
  • The experiment was conducted to study the effect of activated charcoal on the fatty acid composition, meat color and mineral contents of chicken meat with 48 birds of broiler for 6 weeks by adding 0%, 0.6%, 0.9% and 1.2% of activated charcoal to broiler diet. The activated charcoal level of 0.6% and 0.9% increased significantly oleic acid and arachidonic acid contents compared to control(p<0.05). L*, a*, b* values were not influenced by the added level of activated charcoal, however and a* value of different parts of chicken meat was significantly different(p<0.05). The activated charcoal diet increased significantly(p<0.05) the calcium, magnesium and phosphorus content of chicken meat, and tended to increase total mineral contents. In conclusion the activated charcoal supplements to chicken diet increased oleic acid, arachidonic acid the total mineral contents.

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Identification of Greyish White Material Adhered to Shrouds from Tombs Covered with Lime of the Chosun Dynasty (조선시대 회곽 묘 출토 염습의에 부착된 회백색 물질의 동정)

  • OH, Joon-Suk;Yu, Hei-Sun;Yun, Eun-Young
    • Journal of Conservation Science
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    • v.16 s.16
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    • pp.21-26
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    • 2004
  • A lot of waterlogged shrouds and mummies have been found in tombs revered with lime of the Chosun Dynasty(1392-1910) of Korea. Shrouds were heavily polluted with greyish white material. Greyish white material, which adhered to shrouds excavated from tombs(16C and 17C), was submitted to FT-IR qualitative analysis and fatty acid analysis for cleaning. The results of the FT-IR qualitative analysis identified the main ingredient of the greyish white material as calcium salt of fatty acid, including a little of the fatty acids, lipids and proteins. Therefore the greyish white material fumed out adipocere which was formed from the degradation of the lipids in a dead body. From the fatty acid analysis, hydroxy fatty acids(10-Hydroxyhexadecanoic acid and 10-Hydroxyoctadecanoic acid), which were found in adipocere, were detected. The distribution of contents of fatty acids was also rimilar to that of adipocere which have a high proportion of palmitic acid of saturated fatty acid and a low proportion of oleic arid of unsaturated fatty acid. In view of the results of analyses, it was revealed that greyish white material adhered to shrouds was adipocere moved from a mummy.

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The Agronomic Growth Characteristics and Fatty Acid Composition in Genetics Resources of Rapeseed (유채(Brassica napus L.) 유전자원의 생육특성과 지방산 조성)

  • Kwang-Soo Kim;Ji Eun Lee;Young Lok Cha;Da Hee An;Dong Chil Chang
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.83-83
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    • 2022
  • 유채(Brassica napus L.)는 가을에 파종하여 이듬해에 초여름에 수확하는 겨울작물로 종실 수량이 많고 종자의 조지방 함량이 높아 주로 기름을 생산하기 위해서 재배되고 있다. 우리나라에서 유채는 경관을 목적으로 주로 재배되며 면적은 약 5,000ha 정도 재배되고 있다. 최근에는 유채유 생산을 목적으로 전남 등 남부지방에서 재배면적이 증가하고 있다. 유채유의 대량 생산을 위해서는 재배과정의 생력기계화에 유리한 논 재배가 주로 이루어지고 있다. 따라서 유채의 육종은 논 재배 적응성이 뛰어나며, 벼와의 작부가 가능하며 봄 파종 재배가 가능한 조숙품종의 육성이 필요하며, 식용유로 이용이 가능한 지방산 조성이 우수한 품종의 육성도 필요하다. 본 연구는 농촌진흥청 농업유전자원센터에서 보유하고 있는 유채 유전자원 350점을 대상으로 작물학적 생육특성을 평가하였고 종자를 수확한 후 지방산 조성을 분석하였다. 생육특성은 경장 등 12항목을 유채 유전자원 특성조사 및 관리요령(RDA, 2011)을 기준으로 조사하였다. 가을에 파종하여 재배할 때 개화소요일수는 파종 후 137일부터 210일까지 소요되었으며, 봄 파종 재배 시에는 파종 후 65일부터 150일까지 개화가 진행되었고 개화가 되지 않은 계통이 67계통이었다. 경장은 85 ~ 211cm, 수장은 28 ~ 79cm, 분지수는 5 ~ 21개, 수당 협수는 29 ~ 106개, 협당 종자수는 18 ~ 35개 및 협장은 2.7 ~ 8.8cm로 다양하였다. 유채 유전자원의 지방산 중 올레산과 에루스산 함량은 각각 9.7 ~ 70.4% 및 0 ~ 54.7% 범위였다. 공시계료 중 IT 279089 등 3자원은 개화기가 빨라 조생종 육성에, IT279125 등 3자원은 올레산 함량이 68%이상으로 양질 지방산 품종 육성재료로 활용할 예정이다.

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