• Journal of Convergence for Information Technology
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• v.12 no.1
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• pp.119-126
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• 2022

In this study, the antioxidant ability, anti-inflammatory ability and whitening effect of the extracted Grateloupia filicina under optimal conditions were confirmed. The useful substances of Grateloupia filicina were extracted with various ethanol aqueous solutions to measure DPPH, ABTS, and nitrite radical elimination capabilities, and the polyphenol and flavonoid contents were analyzed. When extracted with a 20% ethanol aqueous solution, the radical elimination ability was the best, and the content of polyphenol and flavonoids was also the highest. From this, it was confirmed that the 20% ethanol aqueous solution efficiently extracted the antioxidant and anti-inflammatory substances of Grateloupia filicina. Meanwhile, the cytotoxicity and anti-inflammatory properties of the Grateloupia filicina extract were confirmed using RAW 264.7 cell lines. Grateloupia filicina extract did not show toxicity at a concentration of 200 ㎍/mL, and showed NO production inhibitory capacity of 50% or more at this concentration. As a result, it was confirmed that the Grateloupia filicina extract can be used as a functional cosmetic raw material having antioxidant and anti-inflammatory properties.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.163-168
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• 1976

Three species of Rhodophyceae namely Chondrus ocellatus, Grateloupia filicina and Gigartina tenella were collected from the coast of Haeundae, Busan, on June 33, 1975. And analysed with respect to the content of carrageenan and such chemical characteristics as the content of sulphate and 3, 6-anhydrogalactose, the solubility in potassium chloride solution. In addition, the same chemical properties were tested on the fractions separated by the different concentration of potassium chloride. Carrageenan content in Chondrus ocellatus was relatively higher than two other samples. All the samples showed more than thirty five percent. The Gigartina-carrageenan showed the highest 3,6-anhydrogalactose content among three samples of carrageenan obtained from different species, and the lowest marked from the Grateloupia-carrageenan. In comparison of the solubility of carrageenan in potassium chloride solution, the precipitation yields at 0.125M potassium chloride were marked in order of Chondrus-carrageenan, Grateloupia-carrageenan, and Gigartina-carrageenan, and the yields at 0.125 M to 2.0 M potassium chloride were in Gigartina-carrageenan, Grateloupia-carrageenan, and Chondrus-carrageenan, and the yields from the soluble fraction at 2.0 M potassium chloride were in order of Grateloupia-carrageenan, Gigartina-carrageenan, and Chondrus-carrageenan. It is noteworthy from the result of characteristics of carrageenans that, 3,6-andydrogalactose content was closely related to the solubility of carrageenans in potassium chloride solution and to the sulphate content which might affect reversibly.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.269-270
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• 2000

홍조 지누아리류(Grateloupia spp.)는 독특한 향과 감촉을 가진 유용 해조류로서 강원도 지역에는 옛부터 식용으로 널리 이용되어 왔다. 그러나 산업적 생산량이 자연상태의 생물량에 한정되어 있어서 지역적 한계를 벗어나 그 우수한 풍미를 일반화시키지 못하고 있다. 본 연구에서는 강원도 연안에서 채집한 지누아리사촌(Grateloupia acuminata)의 사분포자체를 조직배양하여 재생체를 유도하고, 이로부터 사분포자를 얻어 배양조건에 따른 포자의 발아와 생장 경향을 파악하였다. 양식에 사용된 종묘는 사분포자로부터 얻은 배우체의 유엽으로서 이의 바다양성을 통하여 대량 양식의 가능성을 파악하고자 하였다. (중략)

• Journal of Aquaculture
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• v.19 no.3
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• pp.216-221
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• 2006

The Effect of different temperatures and photon irradiance on the growth of crust and the regeneration of tissue fragments of the commercially important red alga Grateloupia acuminat Okamura were examined in laboratory cultures. The tetraspore developed into basal crusts and produced upright thalli. Crust grew very fast at $25^{\circ}C$ and $80{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after one week in indoor culture. However, they stopped growing after three weeks. Maximum growth was $275{\mu}m$ in diameter. They required four weeks to get upright thalli at $5^{\circ}C$, while only three weeks were required at $10^{\circ}C$. When different light intensities were compared at $15^{\circ}C$, cells of the crusts were well differentiated $80{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and consistently divided so that upright thalli were produced. In aeration culture, the upright thalli grew up to 6.5 cm in length within 4 months. Thus, it is possible to produce mass cultures of Grateloupia in the field. In addition, female and male gametophytes developed from the tetraspores and they were fertilized to produce tetrasporohyte thalli. By this procedure, the normal life cycle of the red alga G. acuminata was completed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.155-162
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• 1976

Forty one samples from thirty three species of algae (19 from 15 species of Rhodophyceae, 18 from 14 species of Phaeophyceae, 3 from 3 species of Chlorophyceae, and 1 of marine Phanerogams) collected from several locations on the east, west and south coast of Korea, were analyzed for their contents of crude protein, fat, cellulose, ash, nitrogen free extract, amino nitrogen, and total amino acids. For the examination of extractability of algal protein with water, 4 species of algae, Sargassum thunberggi, Grateloupia filicina, Phyllospadix japonica, and Sargassum confusum, were analyzed. And the effect of some precipitation treatments for isolation of algal protein was also tested. As a matter of fact, Rhodophyceae showed high content in crude protein and low in crude fat while the case was opposite for Phaeophyceae and Chlorophyceae. Refering to the content of crude protein and total amino acids, the recommendable algae for protein sources were Sargassum thunbergii, Acrosorium flabellata, Phacelocarpus japonicus, Laurencia okamurai, Laurencia intermedia, Grateloupia filicina, Chondrus ocellatus, Gloiopeltis furcata, Gigartina tenella, Dictyota dichotoma, and Scytosiphon lomentaria. Methanol treatment appeared most effective in precipitation isolation of protein from water extracts whereas pH control method did not so beneficial. The precipitation rate of protein was particularity higher in the extract of Sargassum confusum ana the lowest was makted from the extract of Sargassum thunbergii.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.8
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• pp.1124-1129
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• 2005

Functional activities of low molecular weight substances purified from pepsin hydrolysates of four different seaweeds; Costaria costata, Enteromorpha prolifera, Grateloupia filicina and Porphyra tenera, were inves-tigated. Each pepsin hydrolysate of Costaria costata, Enteromorpha prolifera, and Grateloupia filicina resulted in three peptide peaks on Bio-Rad P2 gel chromatography pattern, while that of Porphyra tenera showed 2 peaks. Peak 1 of Porphyra tenera showed the highest antioxidative activity followed by peak 2 of Porphyra tenera and peak 2 of Costaria costata in order Peak 1 of Porphyra tenera showed the highest ACE inhibitory activity followed by peak 3 and peak 2 of Enteromorpha prolifera in order. Peak 1 and peak 2 of Porphyra tenera, and peak 2 of Enteromorpha prolifera showed the highest antityrosinase activity followed by peak 3 of Enteromorpha prolifera. Peak 1 of Enteromorpha prolifera showed the highest antitumor activity followed by peak 2 of Costaria costata, peak 3 of Enteromorpha prolifera, and peak 3 of Grateloupia filicina in order. Porphyra tenera showed the highest functional activities, which is thought to be due to its high protein content. Structure and amino acid sequence of low molecular weight peptide of Porphyra tenera should be analyzed in the further study.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.8
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• pp.1097-1101
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• 2006

In this study, seven species of seaweeds (Sargassum horneri, Grateloupia filicina, Kjellmaniella crassifolia, Porphyra tenera, Ecklonia stolonifera, Scytosiphon lomentaria, Agarum cribrosum) from Gangwon coast were collected for antioxidative assays. The methanol soluble and insoluble (water soluble) fraction which were prepared from 75% methanol extraction were assayed by DPPH method and deoxyribose assay. The antioxidative activity of methanol soluble fraction of Agarum, Scytosiphon and Sargassum reached to 26.60%, 24.28% and 23.40%, respectively. The antioxidative activities of methanol insoluble fractions of the Kjellmaniella and Porphyra were assayed to 29.70% and 21.01% which were higher than methanol soluble fractions with the values of 9.26% and 8.66%, respectively. The results from DPPH assay and deoxyribose assay showed the methanol soluble fractions of Agarum and Sargassum have strong antioxidative activity. However the methanol insoluble (water soluble) fractions of seaweeds did not show any meaningful antioxidant activity excepted Kjellmaniella and Porphyra.

• Food Engineering Progress
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• v.21 no.1
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• pp.88-92
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• 2017

We attempted to investigate antibacterial and proteolytic activities of bacteria isolated from three ethnic fermented seafoods in the east coast of South Korea, gajami sikhae, squid jeotgal, and fermented jinuari (Grateloupia filicina). Bacillus cereus ATCC 14579, Listeria monocytogenes ATCC 15313, Staphylococcus aureus KCTC 1916, Escherichia coli O157:H7 ATCC 43895, and Salmonella enterica serovar Typhimurium ATCC 4931 were selected to determine the antibacterial activity of the bacterial isolates. Among 233 isolates from the three foods, 36 isolates (15.5%) showed antibacterial activity against B. cereus ATCC 14579, the highest incidence of inhibition, followed by S. aureus KCTC 1916 (7.7%) and L. monocytogenes ATCC 15313 (6.0%). However, only five and three strains among the isolates exhibited inhibitory activity against Gram-negative indicators, E. coli ATCC 43895 and Sal. enterica ATCC 4931, respectively. The proteolytic activity of the isolates was determined via hydrolysis of skim milk after 24, 48, and 72 h incubation. After 72 h incubation, 72 out of 233 isolates (30.9%) showed proteolytic activity, and the isolates of fermented jinuari exhibited the highest incidence of proteolytic activity (60%, 36 isolates). These results suggest that ethnic fermented seafoods in the east coast of South Korea might be a promising source of bacterial strains producing antibacterial and proteolytic compounds.

• Journal of Environmental Science International
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• v.29 no.11
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• pp.1089-1098
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• 2020

To assess the trophic function of an artificial macroalgal habitat, we compared the stable carbon and nitrogen isotope ratios of two amphipods (Caprella sp. and Ampithoe sp.) and their potential food sources in a transplanted macroalgal habitat (Grateloupia asiatica) with those in a natural habitat. There were no significant differences in the isotopic values of both consumers and their potential food sources between the transplanted and natural habitats. Such isotopic similarities between the two sites indicate a comparable resource consumer relationship. Additionally, our results showed similar isotopic niche areas and high dietary overlap (>65%) of the two amphipods between the transplanted and natural sites, suggesting that the transplanted habitat plays ecological roles similar to the natural habitat. Overall, isotopic assessment can provide information on the trophic function of diverse transplanted macroalgal habitats and improve post-monitoring efforts in the management of artificial ecosystems.

• Korean Journal of Food Science and Technology
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• v.23 no.3
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• pp.256-261
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• 1991

To isolate new antioxidants from twelve edible seaweeds, mixed methanol and chloroform extract of marine algae was fractionated into several subfractions and their antioxidant activities were measured by using AOM and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Especially the aqueous-methanol soluble fractions of brown and red algae showed a considerable antioxidant effect. Their antioxidant activities were stronger than synthetic antioxidants such as BHA, BHT, under the same concentration. Further fractionation of the aqueous-methanol soluble fractions using silica gel column chromatography yielded five subfractions. Among them methanol fraction exhibited high DPPH quenching activities. Also, it was confirmed to be benzene-derivative substances of two compounds by UV, HPLC, GC/MS analysis. Its each molecular weight was about 181, 238. These results suggested the existence of two effective natural antioxidant compounds in three edible marine algae.