Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Antibacterial and Proteolytic Activities of Bacterial Isolates from Ethnic Fermented Seafoods in the East Coast of Korea

동해안 특산 수산발효식품에서 분리된 균주의 항균 및 단백질 가수분해 활성

  • Park, Woo Jung (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Lee, Seung Hwan (School of Food Science and Industry, Kyungil University) ;
  • Lee, Hyungjae (Department of Food Engineering, Dankook University)
  • 박우정 (강릉원주대학교 해양식품공학과) ;
  • 이승환 (경일대학교 식품산업융합학부) ;
  • 이형재 (단국대학교 식품공학과)
  • Received : 2016.01.16
  • Accepted : 2017.01.27
  • Published : 2017.02.28
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Abstract

We attempted to investigate antibacterial and proteolytic activities of bacteria isolated from three ethnic fermented seafoods in the east coast of South Korea, gajami sikhae, squid jeotgal, and fermented jinuari (Grateloupia filicina). Bacillus cereus ATCC 14579, Listeria monocytogenes ATCC 15313, Staphylococcus aureus KCTC 1916, Escherichia coli O157:H7 ATCC 43895, and Salmonella enterica serovar Typhimurium ATCC 4931 were selected to determine the antibacterial activity of the bacterial isolates. Among 233 isolates from the three foods, 36 isolates (15.5%) showed antibacterial activity against B. cereus ATCC 14579, the highest incidence of inhibition, followed by S. aureus KCTC 1916 (7.7%) and L. monocytogenes ATCC 15313 (6.0%). However, only five and three strains among the isolates exhibited inhibitory activity against Gram-negative indicators, E. coli ATCC 43895 and Sal. enterica ATCC 4931, respectively. The proteolytic activity of the isolates was determined via hydrolysis of skim milk after 24, 48, and 72 h incubation. After 72 h incubation, 72 out of 233 isolates (30.9%) showed proteolytic activity, and the isolates of fermented jinuari exhibited the highest incidence of proteolytic activity (60%, 36 isolates). These results suggest that ethnic fermented seafoods in the east coast of South Korea might be a promising source of bacterial strains producing antibacterial and proteolytic compounds.

동해안의 특산 수산발효식품 중 가자미밥 식해, 오징어 젓갈, 지누아리 무침 등 총 3종의 수산발효식품에서 233개의 균을 분리하였다. 5종의 주요 식중독 균에 대한 항균활성을 측정한 결과 총 분리균 중 36개(15.5%)가 B. cereus ATCC 14579에 대해 항균활성을 나타내 가장 많은 분리균이 이 지시균을 저해하였다. 그 다음으로 S. aureus KCTC 1916, L. monocytogenes ATCC 15313 순으로 다수의 분리균이 항균활성을 나타냈다. 특히 오징어 젓갈에서 분리한 균주 중 각각 5개(6.0%), 3개(3.6%)가 그람음성 지시균인 E. coli O157:H7 ATCC 43895, Sal. enterica ATCC 4931를 저해하였다. 단백질 가수분해 활성의 경우, 72시간 배양 후, 총 분리균주 중 72개(30.9%)가 활성을 나타냈고, 가장 많은 활성을 나타낸 것은 지누아리 무침 분리균으로 72시간 배양하였을 때 36개(60.0%)가 단백질 가수분해활성을 나타냈다. 본 연구를 통해 수산발효식품에서 분리한 균주가 식중독균을 저해하는 항균물질을 생산한다는 것과 단백질 가수분해 능력을 가진 균주가 존재한다는 것을 알 수 있었다. 활성이 우수한 균주의 확보를 통해 우수 활성균주로부터 천연 항균제를 분리, 적용할 수 있고, 단백질 가수분해활성을 다양한 발효식품 및 더 나아가 저염 수산 발효식품 생산에 활용할 수 있을 것으로 기대된다.

Keywords

Acknowledgement

Grant : 동해안 해양생물자원 유래의 기능성 생물소재 개발

Supported by : 한국해양과학기술진흥원

References

  1. Cho WI, Kim SM. 2012. The biofunctional activities and shelf-life of low-salt squid sikhae. Korean J. Food Sci. Technol. 44: 61-68. https://doi.org/10.9721/KJFST.2012.44.1.061
  2. Choi C, Lee HD, Choi HJ, Son JH, Kim S, Son GM, Cha WS. 2001. Functional and volatile flavor compounds in traditional Kyungsando squid sikhe. Korean J. Food Sci. Technol. 33: 345-352.
  3. Choi YH, Lee JS, Bae SY, Yang KJ, Yeom KW, Jo DH, Kang OH, Baik HS. 2013. Isolation of bacteria with protease activity from Cheonggukjang and purification of fibrinolytic enzyme. J. Life Sci. 23: 259-266. https://doi.org/10.5352/JLS.2013.23.2.259
  4. Guan L, Cho KH, Lee JH. 2011. Analysis of the cultivable bacterial community in jeotgal, a Korean salted and fermented seafood, and identification of its dominant bacteria. Food Microbiol. 28: 101-113.
  5. Han HJ, Kim DG, Han DW, Cho SY. 2014. Effect of natural antimicrobial extracts on shelf-life extension and quality improvement of the flounder sikhe. Korean J. Food Sci. Technol. 46: 689-693. https://doi.org/10.9721/KJFST.2014.46.6.689
  6. Harding CD, Shaw E. 1990. Antimicrobial activity of Leuconostoc gelidum against closely related species and Listeria monocytogenes. J. Appl. Bacteriol. 69: 648-654. https://doi.org/10.1111/j.1365-2672.1990.tb01558.x
  7. Hong WJ, Kim SM. 2013. Quality characteristics, shelf-life, and bioactivities of the low salt squid jeot-gal with natural plant extracts. J. Korean Soc. Food Sci. Nutr. 42: 721-729. https://doi.org/10.3746/jkfn.2013.42.5.721
  8. Hur SH. 1996. Critical review on the microbiological standardization of salt-fermented fish product. J. Korean Soc. Food Sci. Nutr. 25: 885-891.
  9. Kang SY, Oh MJ, Shin JA. 2005. Antimicrobial activities of Korean marine algae against fish pathogenic bacteria. J. Fish Pathol. 18: 147-156.
  10. Kim HJ, Lee NK, Cho SM, Kim KT, Paik HD. 1999. Inhibition of spoilage and pathogenic bacteria by lacticin NK24, a bacteriocin produced by Lactococcus lactis NK24 from fermented fish food. Korean J. Food Sci. Technol. 31: 1035-1043.
  11. Kim MS, Kwon KJ, Lee MJ, Ahn SM, Sohn HY. 2012. Evaluation of the antimicrobial activities of 35 seaweed extracts against pathogenic bacteria and Candida sp. Korean J. Microbiol. Biotechnol. 40: 144-151. https://doi.org/10.4014/kjmb.1203.03005
  12. Kim SJ, Ma SJ, Kim HL. 2005. Probiotic properties of lactic acid bacteria and yeasts isolated from Korean traditional food, Jeotgal. Korean J. Food Preserv. 12: 184-189.
  13. Kim SM. 1996. The effects of food additives on the shelf-life of low-salted myungran-jeot. J. Korean Soc. Food Sci. Nutr. 25: 937-943.
  14. Lee H, Churey JJ, Worobo RW. 2008. Antimicrobial activity of bacterial isolates from different floral sources of honey. Int. J. Food. Microbiol. 126: 240-244. https://doi.org/10.1016/j.ijfoodmicro.2008.04.030
  15. Lee KG, Kim SM. 2012. Quality changes in low-salted squid jeotgal during fermentation and determination of shelf-life. J. Korean Soc. Food Sci. Nutr. 41: 687-694. https://doi.org/10.3746/jkfn.2012.41.5.687
  16. Lee SS, Kim SM, Park UY, Kim HY, Shin IS. 2002. Studies on proteolytic and fibrinolytic activity of Bacillus subtilis JM-3 isolated from anchovy sauce. Korean J. Food Sci. Technol. 34: 283-289.
  17. Lee SY, Song EJ, Kim KBWR, Yoon SY, Kim SJ, Lee SJ, Hong YK, Lim SM, Ahn DH. 2009. Antimicrobial activity of ethanol extract from Sargassum thunbergii. J. Korean Soc. Food Sci. Nutr. 38: 502-508. https://doi.org/10.3746/jkfn.2009.38.4.502
  18. Lee SK, Lee H. 2016. Antimicrobial activity of solvent fractions and bacterial isolates of Korean domestic honey from different floral sources. Food Sci. Biotechnol. 25: 1507-1512. https://doi.org/10.1007/s10068-016-0234-0
  19. Mah JH, Chang YH, Hwang HJ. 2008. Paenibacillus tyraminigenes sp. nov. isolated from myeolchi-jeotgal, a traditional Korean salted and fermented anchovy. Int. J. Food. Microbiol. 127: 209-214. https://doi.org/10.1016/j.ijfoodmicro.2008.07.002
  20. Park BH, Park YH. 1993. Fatty acid composition of salt-fermented seafoods in Chonnam area. J. Korean Soc. Food Sci. Nutr. 22: 465-469.
  21. Roh SW, Nam YD, Chang HW, Sung Y, Kim KH, Oh HM, Bae JW. 2007. Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 57: 2296-2298. https://doi.org/10.1099/ijs.0.65121-0
  22. Suh HK, Yoon SS. 1987. A study on the regional characteristics of Korean chotkal-The kinds and materials of chotkal. Korean J. Diet. Cult. 2: 45-54.
  23. Yoon JH, Jung SY, Kim W, Nam SW, Oh TK. 2006. Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 56: 2587-2592. https://doi.org/10.1099/ijs.0.64266-0
  24. Yoon JH, Yeo SH, Oh TK, Park YH. 2005. Psychrobacter alimentarius sp. nov., isolated from squid jeotgal, a traditional Korean fermented seafood. Int. J. Syst. Evol. Microbiol. 55: 171-176. https://doi.org/10.1099/ijs.0.63140-0

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