• Journal of Satellite, Information and Communications
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• v.12 no.3
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• pp.41-49
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• 2017

This paper will describe lunar lava tubes' five analyzed fields, such as geology, geomorphology, internal configuration, stability, communication and habitats requirements. This research gets through qualitative and qualitative data analysis as following results. A huge size and configuration differences between lunar lava tubes and earth one on geology and landform environments. Exo-genetics activities, such as meteorites, radiation, and sudden temperature bigger affect than Endo-genetic activities, such as effusion and earthquake of the lunar lava tubes. Landform and internal configuration of the lunar lava tubes due to the huge cave perilous landform that gravity difference have a technical limitation from internal approach and data obtain of the huge skylights and sinuous rilles. Stability of the lunar lava tubes deals with geology and landform. It was obvious geo-structural stability elements results generated on low rate of collapsed halls(skylights), low gravity, and relatively thick covers. In terms of the communication capability on the external and internal lunar lava tubes cordless communication techniques will overcome limitations of the sun-power generates supporting communication systems. Through this research it realized obvious differs between potential habitats possibility by accumulative theories by scholars and techniques of the lunar lava tubes. Especially, it is a favorable expectation throughout overcoming attempt on zero gravity, cosmo radiation, lunar dust of the exo-genetic limitations to the steep escarpment of skylights to approach and achieve techniques by the civil engineering, networking and GIS techniques as the endo-genetic environment treatment.

• Korean Journal of Environmental Agriculture
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• v.37 no.2
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• pp.135-140
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• 2018

BACKGROUND: The Asian hornet (Vespa velutina nigrithorax), a wasp species, has attacked honey bee populations and affected the beekeeping industry in Korea over the past 15 years. However, little research has been done with this invasive species. In this study, we investigated the intestine bacterial microbiota of Asian hornets and honey bees to design an attractive trap for Asian hornets. METHODS AND RESULTS: Genomic DNAs isolated from the intestine microorganisms of Asian hornets and honey bees were utilized to amplify bacterial 16S rDNA for the comparative sequence analysis. The next generation sequencing analysis identified that the orders Flavobacteriales as the most abundant intestinal microorganisms in Asian hornets, showing a clear difference compared to honey bees in which Aeromonadales are dominant. We also report five newly identified 16S rDNA sequences of Asian hornet intestinal bacteria. According to the sequence blast search, these five bacteria belong to the genera Thalassomonas, Caedobacter, Vampirovibrio, Alkaliphilus and Calothrix. CONCLUSION: While Asian hornets and honey bees show similar intestine bacterial diversity, the relative ratio of bacterial populations is different. providing useful information to design pest control agents specifically targeting Asian hornets.

• Journal of Life Science
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• v.20 no.12
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• pp.1743-1749
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• 2010

A research group demonstrated that the 37 kDA protein of Edwardsiella tarda, a causing causative agent of edwardsiellosis in fish, exhibited high antigenicity in Japanese flounder. The research group also showed that the N-terminus amino acid sequences of the 37 kDa protein were mapped to the N-terminus of GAPDH (glyceraldehyde-3-phosphate dehydrogenase). Using degenerated primer sets based on the known N-terminus sequence, the corresponding E. tarda DNA was amplified and cloned. The nucleotide sequences of the cloned gene revealed high homology with a bacterial gene for GAPDH, as we was expected. The amino acid sequence of E. tarda GAPDH (etGAPDH) revealed a <70% similarity with GAPDH proteins in other Enterobacteriaceae. With the application of artificial protein overexpression system in Escherichia coli, the recombinant etGAPDH (rGAPDH) was produced and purified. In this study, Using the purified rGAPDH, the etGAPDH specific polyclonal antibody has been was generated using the purified rGAPDHin this study. The immunoblotting analyses demonstrated that the location of the GAPDH protein is located with the association of is associated with the envelops of E. tarda. The rGAPDH was administrated into Japanese flounder via IP route for evaluation of the protective ability. Although the specific antibody titer against etGAPDH was increased about 3-fold after 4 weeks post-vaccination, the survival rates of vaccinated Japanese flounder and the control group with wild type E. tarda was were 12.5% and 0%, respectively. Our results indicated that rGAPDH is immunoreactive antigen but that it will not generate protective immunity in Japanese flounder.

• Journal of Digital Convergence
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• v.18 no.3
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• pp.163-169
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• 2020

A smart key has been used in a variety of embedded environments and there also have been attacks from a remote place by amplifying signals at a location of a user. Existing studies on defence techniques suggest multiple sensors and hash functions to improve authentication speed; these, however, increase the electricity usage and the probability of type 1 error. For these reasons, I suggest an embedded security controller based on client authentication and user movement information improving the authentication method between a controller and a host device. I applied encryption algorithm to the suggested model for communication using an Arduino board, GPS, and Bluetooth and performed authentication through path analysis utilizing user movement information for the authentication. I found that the change in usability was nonsignificant when performing actions using the suggested model by evaluating the time to encode and decode. The embedded security controller in the model can be applied to the system of a remote controller for a two-wheeled vehicle or a mobile and stationary host device; in the process of studying, I found that encryption and decryption could take less then 100ms. The later study may deal with protocols to speed up the data communication including encryption and decryption and the path data management.