Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Intestine Bacterial Microbiota of Asian Hornet (Vespa Velutina Nigrithorax) and Honey Bee

등검은말벌과 꿀벌의 장내 세균 군집 비교

  • Kim, Euyeon (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Seo, Jeongwon (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Yang, So Hee (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, In-Seon (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Koo, Yeonjong (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
  • 김의연 (전남대학교 농업생명과학대학 농화학과) ;
  • 서정원 (전남대학교 농업생명과학대학 농화학과) ;
  • 양소희 (전남대학교 농업생명과학대학 농화학과) ;
  • 김인선 (전남대학교 농업생명과학대학 농화학과) ;
  • 구연종 (전남대학교 농업생명과학대학 농화학과)
  • Received : 2018.06.15
  • Accepted : 2018.06.25
  • Published : 2018.06.30
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Abstract

BACKGROUND: The Asian hornet (Vespa velutina nigrithorax), a wasp species, has attacked honey bee populations and affected the beekeeping industry in Korea over the past 15 years. However, little research has been done with this invasive species. In this study, we investigated the intestine bacterial microbiota of Asian hornets and honey bees to design an attractive trap for Asian hornets. METHODS AND RESULTS: Genomic DNAs isolated from the intestine microorganisms of Asian hornets and honey bees were utilized to amplify bacterial 16S rDNA for the comparative sequence analysis. The next generation sequencing analysis identified that the orders Flavobacteriales as the most abundant intestinal microorganisms in Asian hornets, showing a clear difference compared to honey bees in which Aeromonadales are dominant. We also report five newly identified 16S rDNA sequences of Asian hornet intestinal bacteria. According to the sequence blast search, these five bacteria belong to the genera Thalassomonas, Caedobacter, Vampirovibrio, Alkaliphilus and Calothrix. CONCLUSION: While Asian hornets and honey bees show similar intestine bacterial diversity, the relative ratio of bacterial populations is different. providing useful information to design pest control agents specifically targeting Asian hornets.

한국에서 말벌은 양봉 산업에 큰 영향을 미치는 종이다. 특히 비교적 최근에 전국적으로 번식하는 말벌 종인 등검은말벌 (Vespa velutina nigrithorax)은 작은 체구에도 불구하고 높은 사냥 능력으로 꿀벌을 공격하여 양봉 업계에 큰 피해를 주고 있다. 그러나 이 새로운 침입 종에 대한 연구는 아직 시작단계에 있으며, 본 연구에서는 등검은말벌의 장내 박테리아 군집을 조사하고 이 결과를 꿀벌의 장내 박테리아 군집과 비교하여 등검은말벌의 방제를 위한 자료로 이용하고자 했다. 아시아 말벌과 꿀벌 장의 박테리아 게놈 DNA를 수집하여 16S rDNA를 증폭시켜 가변 부위인 V3, V4 염기서열을 판독 하였다. 계통별 분석결과 목 (order) 수준에서 Flavobacteriales는 등검은말벌에서 가장 우점인 박테리아였고, Aeromonadales와 Pseudomonadales가 그 뒤를 이었다. Flavobacteriaes는 꿀벌의 박테리아 군집과 비교했을 때 등검은말벌에서 증가하였으며, Aeromonadales와 Pseudomonadales는 우점종이지만 꿀벌에 비해 낮은 점유율을 보이는 등 두 개체 간의 차이를 확인할 수 있었다. 한편 이번 연구를 통해 이전에 동정되지 않은 등검은말벌 장 박테리아의 16S rDNA 염기 서열을 분석하였고, 이들 박테리아는 Thalassomonas, Caedobacter, Vampirovibrio, Alkaliphilus 및 Calothrix 속으로 분류되었다.

Keywords

References

  1. Barbet-Massin, M., Rome, Q., Villemant, C., & Courchamp, F. (2018). Can species distribution models really predict the expansion of invasive species?. PLoS One., 13(3), e0193085. https://doi.org/10.1371/journal.pone.0193085
  2. Budge, G. E., Hodgetts, J., Jones, E. P., Ostoja-Starzewski, J. C., Hall, J., Tomkies, V., Semmence, N., Brown, M., Wakefield, M., & Stainton, K. (2017). The invasion, provenance and diversity of Vespa velutina Lepeletier (Hymenoptera: Vespidae) in Great Britain. PLoS One, 12(9), e0185172. https://doi.org/10.1371/journal.pone.0185172
  3. Choi, Y. S., Lee, M. L., Lee, M. Y., Kim, H. K., Yoon, M. Y., & Kang, A. R. (2015). Trapping of vespa velutina nigrithorax Buysson (Hymenoptera: Vespidae) queen near apiaries honeybee comb extract in south region of korea. Journal of Apicultural Research, 30(4), 281-285. https://doi.org/10.17519/apiculture.2015.11.30.4.281
  4. Darrouzet, E., Gevar, J., Guignard, Q., & Aron, S. (2015). Production of early diploid males by european colonies of the invasive hornet Vespa velutina nigrithorax. PLoS One, 10(9), e0136680. https://doi.org/10.1371/journal.pone.0136680
  5. D'Argenio, V., & Salvatore, F. (2015). The role of the gut microbiome in the healthy adult status. Clinica Chimica Acta, 451(part A), 97-102. https://doi.org/10.1016/j.cca.2015.01.003
  6. Kang, E. J., Lee, M. L., Lee, M. Y., Kim, H. K., & Choi, Y. S. (2016). Field evaluation of Honeybee Pheromone traps for the Hornet Vespa velutina nigrithorax Buysson (Hymenoptera: Vespidae). Proceeding of the Apicultural Society of Korea, 116.
  7. Kouduka, M., Suko, T., Morono, Y., Inagaki, F., Ito, K., & Suzuki, Y. (2012). A new DNA extraction method by controlled alkaline treatments from consolidated subsurface sediments. FEMS Microbiology Letters, 326(1), 47-54. https://doi.org/10.1111/j.1574-6968.2011.02437.x
  8. Monceau, K., Bonnard, O., & Thiery, D. (2014). Vespa velutina: a new invasive predator of honeybees in Europe. Journal of Pest Science, 87(1), 1-16. https://doi.org/10.1007/s10340-013-0537-3
  9. Petrosino, J. F., Highlander, S., Luna, R. A., Gibbs, R. A., & Versalovic, J. (2009). Metagenomic pyrosequencing and microbial identification. Clinical chemistry, 55(5), 856-866. https://doi.org/10.1373/clinchem.2008.107565
  10. Poidatz, J., Bressac, C., Bonnard, O., & Thiery, D. (2017). Delayed sexual maturity in males of Vespa velutina. Insect Science, 1-11.
  11. Sauvard, D., Imbault, V., & Darrouzet, E. (2018). Flight capacities of yellow-legged hornet (Vespa velutina nigrithorax, Hymenoptera: Vespidae) workers from an invasive population in Europe. PloS one, 13(6), e0198597. https://doi.org/10.1371/journal.pone.0198597
  12. Sauvard, D., Imbault, V., & Darrouzet, E. (2018). Flight capacities of yellow-legged hornet (Vespa velutina nigrithorax, Hymenoptera: Vespidae) workers from an invasive population in Europe. PloS one, 13(6), e0198597. https://doi.org/10.1371/journal.pone.0198597